The Experts below are selected from a list of 29457 Experts worldwide ranked by ideXlab platform

Xiu-xin Sun - One of the best experts on this subject based on the ideXlab platform.

  • Study on the molecular mechanism of the dissemination of a novel CTX-M-like extended spectrum beta-lactamase-producing Escherichia coil
    2008
    Co-Authors: Jie-hua Jiang, Wei-jiao Liao, Tao Chen, Xiu-xin Sun
    Abstract:

    Objective To investigate the distribution of the CTX-M- extended spectrum beta-lactamase (ESBLs) producing Esche- richia coli(ECO) and the molecular mechanism of dissemination. Methods To analyze the drug resistance of the 43 isolates, Kirby-Bauer susceptibility method was used. Multiple polymeraso chain reaction (PCR) was used to amplify the Gene of ESBLs, AmpC, full length of blaCTX-M-like Gene, Insertion Sequence (IS) ISEcp1B, IS903 , IS26 and integron I. NEST-PCR was used to detect if the beta-lactamase Gene lo- cated in the integron I. The product of full length of bla-CTX-M like gone amplified by PCR was Sequenced. Results Susceptibility test showed the resistance from high to low in turn was Ampicillin (97.68%), Coftriaxone (67.44 % ), piperacillin(65.12 % ), Cefotaxime (62.79 % ) ,Coftasidime(58.14% ), Cofasolin(55.81% ), Cofepime (53.49%), Cefexitin(51.16%), ciprofloxacin (44. 19% ), Aztreo- nam(41.86% ), Cefoperasone/Sulbactam ( 20.93% ), Amikacin (0% ), Imipenem (0% ), respectively. ECO was susceptive to Imipenem. CTX-M-G1 was found in 25 strains of ECO , TEM, SHV, CTX-M-G1, ISEcp1B, and integron I were found in the nine isolates. IS903 were found in ECO 3 and 5, and IS26 was found in ECO 3. In ECO 3 and 5, blaCTX-M-like was flanked upstream by ISEcp1B element that provided -35 and -10 promoter Sequences and a right inverted repeat (IRR) recognized by transposase, downstream by IS903 provided an inverted re- peat, ISEcp1 B and IS903 composed the complex transpeson. Conclusion ISEcplB may drive the expression and dissemination of blaCTX-M-like Gene at a high level. Key words: Beta-lactamases;  Escherichia enli ;  Drug tolerance

Jie-hua Jiang - One of the best experts on this subject based on the ideXlab platform.

  • Study on the molecular mechanism of the dissemination of a novel CTX-M-like extended spectrum beta-lactamase-producing Escherichia coil
    2008
    Co-Authors: Jie-hua Jiang, Wei-jiao Liao, Tao Chen, Xiu-xin Sun
    Abstract:

    Objective To investigate the distribution of the CTX-M- extended spectrum beta-lactamase (ESBLs) producing Esche- richia coli(ECO) and the molecular mechanism of dissemination. Methods To analyze the drug resistance of the 43 isolates, Kirby-Bauer susceptibility method was used. Multiple polymeraso chain reaction (PCR) was used to amplify the Gene of ESBLs, AmpC, full length of blaCTX-M-like Gene, Insertion Sequence (IS) ISEcp1B, IS903 , IS26 and integron I. NEST-PCR was used to detect if the beta-lactamase Gene lo- cated in the integron I. The product of full length of bla-CTX-M like gone amplified by PCR was Sequenced. Results Susceptibility test showed the resistance from high to low in turn was Ampicillin (97.68%), Coftriaxone (67.44 % ), piperacillin(65.12 % ), Cefotaxime (62.79 % ) ,Coftasidime(58.14% ), Cofasolin(55.81% ), Cofepime (53.49%), Cefexitin(51.16%), ciprofloxacin (44. 19% ), Aztreo- nam(41.86% ), Cefoperasone/Sulbactam ( 20.93% ), Amikacin (0% ), Imipenem (0% ), respectively. ECO was susceptive to Imipenem. CTX-M-G1 was found in 25 strains of ECO , TEM, SHV, CTX-M-G1, ISEcp1B, and integron I were found in the nine isolates. IS903 were found in ECO 3 and 5, and IS26 was found in ECO 3. In ECO 3 and 5, blaCTX-M-like was flanked upstream by ISEcp1B element that provided -35 and -10 promoter Sequences and a right inverted repeat (IRR) recognized by transposase, downstream by IS903 provided an inverted re- peat, ISEcp1 B and IS903 composed the complex transpeson. Conclusion ISEcplB may drive the expression and dissemination of blaCTX-M-like Gene at a high level. Key words: Beta-lactamases;  Escherichia enli ;  Drug tolerance

Li Yi-quan - One of the best experts on this subject based on the ideXlab platform.

  • Molecular Mechanism of Novel CTX-M-like Extended Spectrum Beta-lactamase-producing Klebsiella pneumoniae
    Chinese Journal of Nosocomiology, 2009
    Co-Authors: Li Yi-quan
    Abstract:

    OBJECTIVE To investigate molecular machanism of the CTX-M-producing Klebsiella pneumoniae(KPN).METHODS Using K-B susceptibility method to analyze the drug resistance of the 53 isolates;to amplify the Gene of ESBLs,AmpC,full length of blaCTX-M-like Gene,Insertion Sequence(IS)ISEcp1B,IS903,IS26 and integron Ⅰ by multiple polymerase chain reaction(PCR);to detect if the beta-lactamase Gene located in the integron Ⅰ by the nested-PCR;to amplify the full length of blaCTX-M-like Gene in the downstream of ISEcp1B by long-fragment PCR;the PCR product was Sequenced subsequently.RESULTS CTX-M-G1 was found in 17 strains of KPN,ISEcp1B,TEM,SHV,CTX-M-G1 and integronⅠwere found in the six strains;DHA and IS903 were found in KPN 49,9,PSE was found in KPN 9;there was no beta-lactamase Gene located in the integron Ⅰ,dihydrofolate reductase(dhfr) and aminoglycoside-3′-adenylyltransferase(aadA2) Gene located in the integron Ⅰ of Kp49;The 876 bp novel blaCTX-M-Like Gene was found in KPN 49,which differed from blaCTX-M-14 by 1 nucleotide that led to the single amino acid substitution Pro275Gln(GenBank Accession Number:F446126).blaCTX-M-Like Was flanked upstream by an ISEcp1B element provided 35 and 10 promoter Sequences and a right inverted repeat(IRR) recognized by transposase,downstream by an IS903 provided an inverted repeat,ISEcp1B and IS903 composed the complex transposon in KPN 49,9.CONCLUSIONS ISEcp1B may drive the expression and dissemination of blaCTX-M-like Gene at a high level.

Wei-jiao Liao - One of the best experts on this subject based on the ideXlab platform.

  • Study on the molecular mechanism of the dissemination of a novel CTX-M-like extended spectrum beta-lactamase-producing Escherichia coil
    2008
    Co-Authors: Jie-hua Jiang, Wei-jiao Liao, Tao Chen, Xiu-xin Sun
    Abstract:

    Objective To investigate the distribution of the CTX-M- extended spectrum beta-lactamase (ESBLs) producing Esche- richia coli(ECO) and the molecular mechanism of dissemination. Methods To analyze the drug resistance of the 43 isolates, Kirby-Bauer susceptibility method was used. Multiple polymeraso chain reaction (PCR) was used to amplify the Gene of ESBLs, AmpC, full length of blaCTX-M-like Gene, Insertion Sequence (IS) ISEcp1B, IS903 , IS26 and integron I. NEST-PCR was used to detect if the beta-lactamase Gene lo- cated in the integron I. The product of full length of bla-CTX-M like gone amplified by PCR was Sequenced. Results Susceptibility test showed the resistance from high to low in turn was Ampicillin (97.68%), Coftriaxone (67.44 % ), piperacillin(65.12 % ), Cefotaxime (62.79 % ) ,Coftasidime(58.14% ), Cofasolin(55.81% ), Cofepime (53.49%), Cefexitin(51.16%), ciprofloxacin (44. 19% ), Aztreo- nam(41.86% ), Cefoperasone/Sulbactam ( 20.93% ), Amikacin (0% ), Imipenem (0% ), respectively. ECO was susceptive to Imipenem. CTX-M-G1 was found in 25 strains of ECO , TEM, SHV, CTX-M-G1, ISEcp1B, and integron I were found in the nine isolates. IS903 were found in ECO 3 and 5, and IS26 was found in ECO 3. In ECO 3 and 5, blaCTX-M-like was flanked upstream by ISEcp1B element that provided -35 and -10 promoter Sequences and a right inverted repeat (IRR) recognized by transposase, downstream by IS903 provided an inverted re- peat, ISEcp1 B and IS903 composed the complex transpeson. Conclusion ISEcplB may drive the expression and dissemination of blaCTX-M-like Gene at a high level. Key words: Beta-lactamases;  Escherichia enli ;  Drug tolerance

Tao Chen - One of the best experts on this subject based on the ideXlab platform.

  • Study on the molecular mechanism of the dissemination of a novel CTX-M-like extended spectrum beta-lactamase-producing Escherichia coil
    2008
    Co-Authors: Jie-hua Jiang, Wei-jiao Liao, Tao Chen, Xiu-xin Sun
    Abstract:

    Objective To investigate the distribution of the CTX-M- extended spectrum beta-lactamase (ESBLs) producing Esche- richia coli(ECO) and the molecular mechanism of dissemination. Methods To analyze the drug resistance of the 43 isolates, Kirby-Bauer susceptibility method was used. Multiple polymeraso chain reaction (PCR) was used to amplify the Gene of ESBLs, AmpC, full length of blaCTX-M-like Gene, Insertion Sequence (IS) ISEcp1B, IS903 , IS26 and integron I. NEST-PCR was used to detect if the beta-lactamase Gene lo- cated in the integron I. The product of full length of bla-CTX-M like gone amplified by PCR was Sequenced. Results Susceptibility test showed the resistance from high to low in turn was Ampicillin (97.68%), Coftriaxone (67.44 % ), piperacillin(65.12 % ), Cefotaxime (62.79 % ) ,Coftasidime(58.14% ), Cofasolin(55.81% ), Cofepime (53.49%), Cefexitin(51.16%), ciprofloxacin (44. 19% ), Aztreo- nam(41.86% ), Cefoperasone/Sulbactam ( 20.93% ), Amikacin (0% ), Imipenem (0% ), respectively. ECO was susceptive to Imipenem. CTX-M-G1 was found in 25 strains of ECO , TEM, SHV, CTX-M-G1, ISEcp1B, and integron I were found in the nine isolates. IS903 were found in ECO 3 and 5, and IS26 was found in ECO 3. In ECO 3 and 5, blaCTX-M-like was flanked upstream by ISEcp1B element that provided -35 and -10 promoter Sequences and a right inverted repeat (IRR) recognized by transposase, downstream by IS903 provided an inverted re- peat, ISEcp1 B and IS903 composed the complex transpeson. Conclusion ISEcplB may drive the expression and dissemination of blaCTX-M-like Gene at a high level. Key words: Beta-lactamases;  Escherichia enli ;  Drug tolerance