Gene Sequence

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Mo Yang - One of the best experts on this subject based on the ideXlab platform.

  • a fluorescence resonance energy transfer fret biosensor based on graphene quantum dots gqds and gold nanoparticles aunps for the detection of meca Gene Sequence of staphylococcus aureus
    Biosensors and Bioelectronics, 2015
    Co-Authors: Jingyu Shi, Chunyu Chan, Yukting Pang, Feng Tian, Jing Lyu, Yu Zhang, Mo Yang
    Abstract:

    In this work, a novel fluorescence resonance energy transfer (FRET) biosensor based on graphene quantum dots (GQDs) and gold nanoparticles (AuNPs) pairs was developed for Staphylococcus aureus specific Gene Sequence detection. This FRET biosensor platform was realized by immobilization of capture probes on GQDs and conjugation of reporter probes on AuNPs. Target oligos then co-hybridized with capture probes and reporter probes to form a sandwich structure which brought GQDs and AuNPs to close proximity to trigger FRET effect. The fluorescence signals before and after addition of targets were measured and the fluorescence quenching efficiency could reach around 87% with 100 nM target oligo. The limit of detection (LOD) of this FRET biosensor was around 1 nM for S.aureus Gene detection. Experiments with both single-base mismatched oligos and double-base mismatched oligos demonstrated the good Sequence selectivity of this FRET biosensor.

  • a fluorescence resonance energy transfer fret biosensor based on graphene quantum dots gqds and gold nanoparticles aunps for the detection of meca Gene Sequence of staphylococcus aureus
    Biosensors and Bioelectronics, 2015
    Co-Authors: Chunyu Chan, Yukting Pang, Feng Tian, Yu Zhang, Weiwei Ye, Mo Yang
    Abstract:

    Abstract In this work, a novel fluorescence resonance energy transfer (FRET) biosensor based on graphene quantum dots (GQDs) and gold nanoparticles (AuNPs) pairs was developed for Staphylococcus aureus specific Gene Sequence detection. This FRET biosensor platform was realized by immobilization of capture probes on GQDs and conjugation of reporter probes on AuNPs. Target oligos then co-hybridized with capture probes and reporter probes to form a sandwich structure which brought GQDs and AuNPs to close proximity to trigger FRET effect. The fluorescence signals before and after addition of targets were measured and the fluorescence quenching efficiency could reach around 87% with 100 nM target oligo. The limit of detection (LOD) of this FRET biosensor was around 1 nM for S.aureus Gene detection. Experiments with both single-base mismatched oligos and double-base mismatched oligos demonstrated the good Sequence selectivity of this FRET biosensor.

Chunyu Chan - One of the best experts on this subject based on the ideXlab platform.

  • a fluorescence resonance energy transfer fret biosensor based on graphene quantum dots gqds and gold nanoparticles aunps for the detection of meca Gene Sequence of staphylococcus aureus
    Biosensors and Bioelectronics, 2015
    Co-Authors: Jingyu Shi, Chunyu Chan, Yukting Pang, Feng Tian, Jing Lyu, Yu Zhang, Mo Yang
    Abstract:

    In this work, a novel fluorescence resonance energy transfer (FRET) biosensor based on graphene quantum dots (GQDs) and gold nanoparticles (AuNPs) pairs was developed for Staphylococcus aureus specific Gene Sequence detection. This FRET biosensor platform was realized by immobilization of capture probes on GQDs and conjugation of reporter probes on AuNPs. Target oligos then co-hybridized with capture probes and reporter probes to form a sandwich structure which brought GQDs and AuNPs to close proximity to trigger FRET effect. The fluorescence signals before and after addition of targets were measured and the fluorescence quenching efficiency could reach around 87% with 100 nM target oligo. The limit of detection (LOD) of this FRET biosensor was around 1 nM for S.aureus Gene detection. Experiments with both single-base mismatched oligos and double-base mismatched oligos demonstrated the good Sequence selectivity of this FRET biosensor.

  • a fluorescence resonance energy transfer fret biosensor based on graphene quantum dots gqds and gold nanoparticles aunps for the detection of meca Gene Sequence of staphylococcus aureus
    Biosensors and Bioelectronics, 2015
    Co-Authors: Chunyu Chan, Yukting Pang, Feng Tian, Yu Zhang, Weiwei Ye, Mo Yang
    Abstract:

    Abstract In this work, a novel fluorescence resonance energy transfer (FRET) biosensor based on graphene quantum dots (GQDs) and gold nanoparticles (AuNPs) pairs was developed for Staphylococcus aureus specific Gene Sequence detection. This FRET biosensor platform was realized by immobilization of capture probes on GQDs and conjugation of reporter probes on AuNPs. Target oligos then co-hybridized with capture probes and reporter probes to form a sandwich structure which brought GQDs and AuNPs to close proximity to trigger FRET effect. The fluorescence signals before and after addition of targets were measured and the fluorescence quenching efficiency could reach around 87% with 100 nM target oligo. The limit of detection (LOD) of this FRET biosensor was around 1 nM for S.aureus Gene detection. Experiments with both single-base mismatched oligos and double-base mismatched oligos demonstrated the good Sequence selectivity of this FRET biosensor.

Yu Zhang - One of the best experts on this subject based on the ideXlab platform.

  • a fluorescence resonance energy transfer fret biosensor based on graphene quantum dots gqds and gold nanoparticles aunps for the detection of meca Gene Sequence of staphylococcus aureus
    Biosensors and Bioelectronics, 2015
    Co-Authors: Jingyu Shi, Chunyu Chan, Yukting Pang, Feng Tian, Jing Lyu, Yu Zhang, Mo Yang
    Abstract:

    In this work, a novel fluorescence resonance energy transfer (FRET) biosensor based on graphene quantum dots (GQDs) and gold nanoparticles (AuNPs) pairs was developed for Staphylococcus aureus specific Gene Sequence detection. This FRET biosensor platform was realized by immobilization of capture probes on GQDs and conjugation of reporter probes on AuNPs. Target oligos then co-hybridized with capture probes and reporter probes to form a sandwich structure which brought GQDs and AuNPs to close proximity to trigger FRET effect. The fluorescence signals before and after addition of targets were measured and the fluorescence quenching efficiency could reach around 87% with 100 nM target oligo. The limit of detection (LOD) of this FRET biosensor was around 1 nM for S.aureus Gene detection. Experiments with both single-base mismatched oligos and double-base mismatched oligos demonstrated the good Sequence selectivity of this FRET biosensor.

  • a fluorescence resonance energy transfer fret biosensor based on graphene quantum dots gqds and gold nanoparticles aunps for the detection of meca Gene Sequence of staphylococcus aureus
    Biosensors and Bioelectronics, 2015
    Co-Authors: Chunyu Chan, Yukting Pang, Feng Tian, Yu Zhang, Weiwei Ye, Mo Yang
    Abstract:

    Abstract In this work, a novel fluorescence resonance energy transfer (FRET) biosensor based on graphene quantum dots (GQDs) and gold nanoparticles (AuNPs) pairs was developed for Staphylococcus aureus specific Gene Sequence detection. This FRET biosensor platform was realized by immobilization of capture probes on GQDs and conjugation of reporter probes on AuNPs. Target oligos then co-hybridized with capture probes and reporter probes to form a sandwich structure which brought GQDs and AuNPs to close proximity to trigger FRET effect. The fluorescence signals before and after addition of targets were measured and the fluorescence quenching efficiency could reach around 87% with 100 nM target oligo. The limit of detection (LOD) of this FRET biosensor was around 1 nM for S.aureus Gene detection. Experiments with both single-base mismatched oligos and double-base mismatched oligos demonstrated the good Sequence selectivity of this FRET biosensor.

Yukting Pang - One of the best experts on this subject based on the ideXlab platform.

  • a fluorescence resonance energy transfer fret biosensor based on graphene quantum dots gqds and gold nanoparticles aunps for the detection of meca Gene Sequence of staphylococcus aureus
    Biosensors and Bioelectronics, 2015
    Co-Authors: Jingyu Shi, Chunyu Chan, Yukting Pang, Feng Tian, Jing Lyu, Yu Zhang, Mo Yang
    Abstract:

    In this work, a novel fluorescence resonance energy transfer (FRET) biosensor based on graphene quantum dots (GQDs) and gold nanoparticles (AuNPs) pairs was developed for Staphylococcus aureus specific Gene Sequence detection. This FRET biosensor platform was realized by immobilization of capture probes on GQDs and conjugation of reporter probes on AuNPs. Target oligos then co-hybridized with capture probes and reporter probes to form a sandwich structure which brought GQDs and AuNPs to close proximity to trigger FRET effect. The fluorescence signals before and after addition of targets were measured and the fluorescence quenching efficiency could reach around 87% with 100 nM target oligo. The limit of detection (LOD) of this FRET biosensor was around 1 nM for S.aureus Gene detection. Experiments with both single-base mismatched oligos and double-base mismatched oligos demonstrated the good Sequence selectivity of this FRET biosensor.

  • a fluorescence resonance energy transfer fret biosensor based on graphene quantum dots gqds and gold nanoparticles aunps for the detection of meca Gene Sequence of staphylococcus aureus
    Biosensors and Bioelectronics, 2015
    Co-Authors: Chunyu Chan, Yukting Pang, Feng Tian, Yu Zhang, Weiwei Ye, Mo Yang
    Abstract:

    Abstract In this work, a novel fluorescence resonance energy transfer (FRET) biosensor based on graphene quantum dots (GQDs) and gold nanoparticles (AuNPs) pairs was developed for Staphylococcus aureus specific Gene Sequence detection. This FRET biosensor platform was realized by immobilization of capture probes on GQDs and conjugation of reporter probes on AuNPs. Target oligos then co-hybridized with capture probes and reporter probes to form a sandwich structure which brought GQDs and AuNPs to close proximity to trigger FRET effect. The fluorescence signals before and after addition of targets were measured and the fluorescence quenching efficiency could reach around 87% with 100 nM target oligo. The limit of detection (LOD) of this FRET biosensor was around 1 nM for S.aureus Gene detection. Experiments with both single-base mismatched oligos and double-base mismatched oligos demonstrated the good Sequence selectivity of this FRET biosensor.

Feng Tian - One of the best experts on this subject based on the ideXlab platform.

  • a fluorescence resonance energy transfer fret biosensor based on graphene quantum dots gqds and gold nanoparticles aunps for the detection of meca Gene Sequence of staphylococcus aureus
    Biosensors and Bioelectronics, 2015
    Co-Authors: Jingyu Shi, Chunyu Chan, Yukting Pang, Feng Tian, Jing Lyu, Yu Zhang, Mo Yang
    Abstract:

    In this work, a novel fluorescence resonance energy transfer (FRET) biosensor based on graphene quantum dots (GQDs) and gold nanoparticles (AuNPs) pairs was developed for Staphylococcus aureus specific Gene Sequence detection. This FRET biosensor platform was realized by immobilization of capture probes on GQDs and conjugation of reporter probes on AuNPs. Target oligos then co-hybridized with capture probes and reporter probes to form a sandwich structure which brought GQDs and AuNPs to close proximity to trigger FRET effect. The fluorescence signals before and after addition of targets were measured and the fluorescence quenching efficiency could reach around 87% with 100 nM target oligo. The limit of detection (LOD) of this FRET biosensor was around 1 nM for S.aureus Gene detection. Experiments with both single-base mismatched oligos and double-base mismatched oligos demonstrated the good Sequence selectivity of this FRET biosensor.

  • a fluorescence resonance energy transfer fret biosensor based on graphene quantum dots gqds and gold nanoparticles aunps for the detection of meca Gene Sequence of staphylococcus aureus
    Biosensors and Bioelectronics, 2015
    Co-Authors: Chunyu Chan, Yukting Pang, Feng Tian, Yu Zhang, Weiwei Ye, Mo Yang
    Abstract:

    Abstract In this work, a novel fluorescence resonance energy transfer (FRET) biosensor based on graphene quantum dots (GQDs) and gold nanoparticles (AuNPs) pairs was developed for Staphylococcus aureus specific Gene Sequence detection. This FRET biosensor platform was realized by immobilization of capture probes on GQDs and conjugation of reporter probes on AuNPs. Target oligos then co-hybridized with capture probes and reporter probes to form a sandwich structure which brought GQDs and AuNPs to close proximity to trigger FRET effect. The fluorescence signals before and after addition of targets were measured and the fluorescence quenching efficiency could reach around 87% with 100 nM target oligo. The limit of detection (LOD) of this FRET biosensor was around 1 nM for S.aureus Gene detection. Experiments with both single-base mismatched oligos and double-base mismatched oligos demonstrated the good Sequence selectivity of this FRET biosensor.