The Experts below are selected from a list of 267 Experts worldwide ranked by ideXlab platform

Kinji Asaka - One of the best experts on this subject based on the ideXlab platform.

  • Electrochemical and Electromechanical Properties of Activated Multi-walled Carbon Nanotube Polymer Actuator that Surpass the Performance of a Single-walled Carbon Nanotube Polymer Actuator
    Materials Today: Proceedings, 2016
    Co-Authors: Naohiro Terasawa, Kinji Asaka
    Abstract:

    The electrochemical and electromechanical properties of actuators fabricated from activated and non-activated multi-walled carbon nanotube (MWCNT)-ionic liquid (IL) gel electrodes are compared with single-walled carbon nanotube (SWCNT)-based actuators. The double-layer capacitance of the MWCNT-COOH electrode was larger than that of the MWCNT electrode but smaller than that of the SWCNT electrode. The activated MWCNT-COOH polymer actuator surpassed the SWCNT actuator in terms of the strain and maximum Generated Stress.

  • Improved performance of an activated multi-walled carbon nanotube polymer actuator, compared with a single-walled carbon nanotube polymer actuator
    Sensors and Actuators B-chemical, 2012
    Co-Authors: Naohiro Terasawa, Ken Mukai, Kinji Asaka
    Abstract:

    Abstract Actuators were developed using activated multi-walled carbon nanotube (MWCNT)–ionic liquid (IL) gel electrodes, and were compared with non-activated MWCNT- and single-walled carbon nanotube (SWCNT)-based actuators, in terms of their electrochemical and electromechanical properties. Furthermore, the effects of variations in the IL on the electrochemical and electromechanical properties of the activated MWCNT–NH2/IL gel electrode actuators were investigated. The performance of the activated MWCNT–NH2 polymer actuator surpassed that of the SWCNT actuator, in terms of the strain and maximum Generated Stress. For the MWCNT–NH2 actuators, the strain and maximum Generated Stress were dependent on the IL species.

  • High performance polymer actuators based on multi-walled carbon nanotubes that surpass the performance of those containing single-walled carbon nanotubes: Effects of ionic liquid and composition
    Sensors and Actuators B: Chemical, 2012
    Co-Authors: Naohiro Terasawa, Norihiro Ono, Ken Mukai, Tomoyuki Koga, Nobuyuki Higashi, Kinji Asaka
    Abstract:

    The effects of ionic liquid (IL) and composition (ratio of carbon nanotube (CNT):polymer:IL) on the electrochemical and electromechanical properties of actuators containing activated and non-activated multi-walled carbon nanotube (MWCNT)-IL gel electrodes were investigated. The electrochemical and electromechanical properties of actuators containing the activated and non-activated MWCNT-IL gel electrodes were compared to those of a single-walled carbon nanotube (SWCNT)-based actuator. The double-layer capacitance of the activated MWCNT (MWCNT-COOH) electrode and the strain and maximum Generated Stress for the MWCNT-COOH or MWCNT actuator are dependent on both the IL and composition. For the MWCNT-COOH actuator containing 1-ethyl-3-methylimidazolium tetrafluoroborate (EMI[BF 4]) (MWCNT-COOH:poly(vinylidene fluoride-co-hexafluoropropylene):EMI[BF 4] = 30:30:35), the strain was 0.78-0.80% for the frequency range of 0.01-0.005 Hz, which was ca. twice that measured for the SWCNT actuator, and the maximum Generated Stress was ca. 1.8-2.5 times larger than that observed for the SWCNT actuator. Therefore, the common CNT activated MWCNT actuator can generate a maximum Stress sufficient for practical purposes without using specialized SWCNT. Furthermore, the actuator containing MWCNT-COOH performed better than those containing SWCNTs or MWCNTs, and gave a more rapid response. © 2011 Elsevier B.V. All rights reserved.

  • Improved performance of an activated multi-walled carbon nanotube polymer actuator, compared with a single-walled carbon nanotube polymer actuator
    Sensors and Actuators B: Chemical, 2012
    Co-Authors: Naohiro Terasawa, Ken Mukai, Kinji Asaka
    Abstract:

    Actuators were developed using activated multi-walled carbon nanotube (MWCNT)-ionic liquid (IL) gel electrodes, and were compared with non-activated MWCNT- and single-walled carbon nanotube (SWCNT)-based actuators, in terms of their electrochemical and electromechanical properties. Furthermore, the effects of variations in the IL on the electrochemical and electromechanical properties of the activated MWCNT-NH 2/IL gel electrode actuators were investigated. The performance of the activated MWCNT-NH 2 polymer actuator surpassed that of the SWCNT actuator, in terms of the strain and maximum Generated Stress. For the MWCNT-NH 2 actuators, the strain and maximum Generated Stress were dependent on the IL species. © 2012 Elsevier B.V.

  • Superior performance of non-activated multi-walled carbon nanotube polymer actuator containing ruthenium oxide over a single-walled carbon nanotube
    Carbon, 2012
    Co-Authors: Naohiro Terasawa, Ken Mukai, Kentaro Yamato, Kinji Asaka
    Abstract:

    The electrochemical and electromechanical properties of actuators developed using a non-activated multi-walled carbon nanotube (MWCNT)-ionic liquid (IL) gel electrode containing ruthenium oxide (RuO 2) were compared with only-MWCNT and only-single-walled carbon nanotube (SWCNT) based actuators. The double-layer capacitance of the non-activated MWCNT electrode containing RuO 2 was larger than that of the only-MWCNT electrode. The non-activated MWCNT polymer actuator containing RuO 2 surpassed the performance of the only-MWCNT and only-SWCNT actuators in terms of the strain and maximum Generated Stress. Both MWCNTs and RuO 2 were required to produce large strain and quick response actuators that surpassed the performance of the only-SWCNT polymer actuator and exhibited characteristics sufficient for practical applications (e.g. tactile display). © 2012 Elsevier Ltd. All rights reserved.

Takahiro Ogawa - One of the best experts on this subject based on the ideXlab platform.

  • glycosaminoglycan degradation reduces mineralized tissue titanium interfacial strength
    Journal of Biomedical Materials Research Part A, 2006
    Co-Authors: Hiromi Nakamura, Hideki Aita, Frank Butz, Jaewoo Shim, Vijay Gupta, Takahiro Ogawa
    Abstract:

    Although the localization of the proteoglycan/glycosaminoglycan (GAG) complex at the bone–titanium implant interface has been implied, the role of proteoglycans on the establishment of bone–titanium integration is unknown. The hypothesis to be tested was that proteoglycans play an important role in establishing bone–titanium interfacial adhesion. The objective of this study is to investigate the effect of proteoglycan knockdown by GAG enzymatic degradation on the interfacial strength between mineralized tissue and titanium having different surface topographies. Rat bone marrow-derived osteoblastic cells were cultured on either a machined titanium disk or an acid-etched titanium disk. At day 21 of culture, one of the three following GAG degradation enzymes was added into the culture; chondroitinase AC, chondroitinase B, or keratanase. After 3 days of incubation (at day 24 of culture), the laser spallation technique was applied to the samples in order to assess the tissue–titanium interfacial strength. In this technique, a laser-Generated Stress wave is used to separate the tissue–titanium interface, and the interfacial strength is determined interferometrically by recording the transient free surface velocity of the tissue. Mineralized tissue cultured on the acid-etched titanium showed 20–30% higher tissue interfacial strength than that cultured on the machined titanium (p < 0.0001). For both the machined and acid-etched surface cultures, administration of the enzyme reduced the interfacial strength by 25–30% compared with the untreated control cultures (p < 0.0001). There were no differences in the effect among the three different enzymes tested. A nanoindentation study revealed that the enzyme treatment did not affect the elastic modulus of the mineralized tissue. Scanning electron microscopic and energy dispersive spectroscopic analyses revealed less post-spallation tissue remnant on the titanium substrates when treated with the enzymes. The tissue remnant was greater in amount on the acid-etched surface than on the machined surface. The results suggest that there exists not only mechanical interlocking but also biological interfacial adhesion between the mineralized tissue and titanium, in which the proteoglycan/GAG complex is involved. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res, 2006

  • Glycosaminoglycan degradation reduces mineralized tissue–titanium interfacial strength
    Journal of Biomedical Materials Research Part A, 2006
    Co-Authors: Hiromi Nakamura, Hideki Aita, Frank Butz, Jaewoo Shim, Vijay Gupta, Takahiro Ogawa
    Abstract:

    Although the localization of the proteoglycan/glycosaminoglycan (GAG) complex at the bone–titanium implant interface has been implied, the role of proteoglycans on the establishment of bone–titanium integration is unknown. The hypothesis to be tested was that proteoglycans play an important role in establishing bone–titanium interfacial adhesion. The objective of this study is to investigate the effect of proteoglycan knockdown by GAG enzymatic degradation on the interfacial strength between mineralized tissue and titanium having different surface topographies. Rat bone marrow-derived osteoblastic cells were cultured on either a machined titanium disk or an acid-etched titanium disk. At day 21 of culture, one of the three following GAG degradation enzymes was added into the culture; chondroitinase AC, chondroitinase B, or keratanase. After 3 days of incubation (at day 24 of culture), the laser spallation technique was applied to the samples in order to assess the tissue–titanium interfacial strength. In this technique, a laser-Generated Stress wave is used to separate the tissue–titanium interface, and the interfacial strength is determined interferometrically by recording the transient free surface velocity of the tissue. Mineralized tissue cultured on the acid-etched titanium showed 20–30% higher tissue interfacial strength than that cultured on the machined titanium (p < 0.0001). For both the machined and acid-etched surface cultures, administration of the enzyme reduced the interfacial strength by 25–30% compared with the untreated control cultures (p < 0.0001). There were no differences in the effect among the three different enzymes tested. A nanoindentation study revealed that the enzyme treatment did not affect the elastic modulus of the mineralized tissue. Scanning electron microscopic and energy dispersive spectroscopic analyses revealed less post-spallation tissue remnant on the titanium substrates when treated with the enzymes. The tissue remnant was greater in amount on the acid-etched surface than on the machined surface. The results suggest that there exists not only mechanical interlocking but also biological interfacial adhesion between the mineralized tissue and titanium, in which the proteoglycan/GAG complex is involved. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res, 2006

Vijay Gupta - One of the best experts on this subject based on the ideXlab platform.

  • glycosaminoglycan degradation reduces mineralized tissue titanium interfacial strength
    Journal of Biomedical Materials Research Part A, 2006
    Co-Authors: Hiromi Nakamura, Hideki Aita, Frank Butz, Jaewoo Shim, Vijay Gupta, Takahiro Ogawa
    Abstract:

    Although the localization of the proteoglycan/glycosaminoglycan (GAG) complex at the bone–titanium implant interface has been implied, the role of proteoglycans on the establishment of bone–titanium integration is unknown. The hypothesis to be tested was that proteoglycans play an important role in establishing bone–titanium interfacial adhesion. The objective of this study is to investigate the effect of proteoglycan knockdown by GAG enzymatic degradation on the interfacial strength between mineralized tissue and titanium having different surface topographies. Rat bone marrow-derived osteoblastic cells were cultured on either a machined titanium disk or an acid-etched titanium disk. At day 21 of culture, one of the three following GAG degradation enzymes was added into the culture; chondroitinase AC, chondroitinase B, or keratanase. After 3 days of incubation (at day 24 of culture), the laser spallation technique was applied to the samples in order to assess the tissue–titanium interfacial strength. In this technique, a laser-Generated Stress wave is used to separate the tissue–titanium interface, and the interfacial strength is determined interferometrically by recording the transient free surface velocity of the tissue. Mineralized tissue cultured on the acid-etched titanium showed 20–30% higher tissue interfacial strength than that cultured on the machined titanium (p < 0.0001). For both the machined and acid-etched surface cultures, administration of the enzyme reduced the interfacial strength by 25–30% compared with the untreated control cultures (p < 0.0001). There were no differences in the effect among the three different enzymes tested. A nanoindentation study revealed that the enzyme treatment did not affect the elastic modulus of the mineralized tissue. Scanning electron microscopic and energy dispersive spectroscopic analyses revealed less post-spallation tissue remnant on the titanium substrates when treated with the enzymes. The tissue remnant was greater in amount on the acid-etched surface than on the machined surface. The results suggest that there exists not only mechanical interlocking but also biological interfacial adhesion between the mineralized tissue and titanium, in which the proteoglycan/GAG complex is involved. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res, 2006

  • Glycosaminoglycan degradation reduces mineralized tissue–titanium interfacial strength
    Journal of Biomedical Materials Research Part A, 2006
    Co-Authors: Hiromi Nakamura, Hideki Aita, Frank Butz, Jaewoo Shim, Vijay Gupta, Takahiro Ogawa
    Abstract:

    Although the localization of the proteoglycan/glycosaminoglycan (GAG) complex at the bone–titanium implant interface has been implied, the role of proteoglycans on the establishment of bone–titanium integration is unknown. The hypothesis to be tested was that proteoglycans play an important role in establishing bone–titanium interfacial adhesion. The objective of this study is to investigate the effect of proteoglycan knockdown by GAG enzymatic degradation on the interfacial strength between mineralized tissue and titanium having different surface topographies. Rat bone marrow-derived osteoblastic cells were cultured on either a machined titanium disk or an acid-etched titanium disk. At day 21 of culture, one of the three following GAG degradation enzymes was added into the culture; chondroitinase AC, chondroitinase B, or keratanase. After 3 days of incubation (at day 24 of culture), the laser spallation technique was applied to the samples in order to assess the tissue–titanium interfacial strength. In this technique, a laser-Generated Stress wave is used to separate the tissue–titanium interface, and the interfacial strength is determined interferometrically by recording the transient free surface velocity of the tissue. Mineralized tissue cultured on the acid-etched titanium showed 20–30% higher tissue interfacial strength than that cultured on the machined titanium (p < 0.0001). For both the machined and acid-etched surface cultures, administration of the enzyme reduced the interfacial strength by 25–30% compared with the untreated control cultures (p < 0.0001). There were no differences in the effect among the three different enzymes tested. A nanoindentation study revealed that the enzyme treatment did not affect the elastic modulus of the mineralized tissue. Scanning electron microscopic and energy dispersive spectroscopic analyses revealed less post-spallation tissue remnant on the titanium substrates when treated with the enzymes. The tissue remnant was greater in amount on the acid-etched surface than on the machined surface. The results suggest that there exists not only mechanical interlocking but also biological interfacial adhesion between the mineralized tissue and titanium, in which the proteoglycan/GAG complex is involved. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res, 2006

  • measurement of thin film interface toughness by using laser Generated Stress pulses
    Journal of The Mechanics and Physics of Solids, 1998
    Co-Authors: A N Pronin, Vijay Gupta
    Abstract:

    Abstract A new technique to measure the toughness of interfaces is presented. In this experiment a laser-Generated compressive Stress pulse on the back surface of a substrate disc is made to propagate towards a thin film interface containing a population of well characterized microcracks. The compressive Stress pulse reflects into a tensile pulse from the coating's free surface and leads to the dynamic initiation of one of the cracks. The coating's free surface velocity at crack initiation is measured by using an optical interferometer, and related to the local energy release rate (equal to interface toughness) for a prescribed flaw geometry by using a numerical simulation based on dynamic fracture. Both penny-shaped and line interfacial microcracks were Generated by combining photolithography and encouraging layer buckling from selected spots under the influence of their own residual compressive Stresses. The experimental strategy is demonstrated on interfaces between sapphire substrates with (0001) plane orientations and coatings of Al, Cr, Nb and Sb for which the interface toughnesses of 1.3, 0.8, 0.6 and 0.04 J/m 2 were obtained, respectively.

Naohiro Terasawa - One of the best experts on this subject based on the ideXlab platform.

  • Electrochemical and Electromechanical Properties of Activated Multi-walled Carbon Nanotube Polymer Actuator that Surpass the Performance of a Single-walled Carbon Nanotube Polymer Actuator
    Materials Today: Proceedings, 2016
    Co-Authors: Naohiro Terasawa, Kinji Asaka
    Abstract:

    The electrochemical and electromechanical properties of actuators fabricated from activated and non-activated multi-walled carbon nanotube (MWCNT)-ionic liquid (IL) gel electrodes are compared with single-walled carbon nanotube (SWCNT)-based actuators. The double-layer capacitance of the MWCNT-COOH electrode was larger than that of the MWCNT electrode but smaller than that of the SWCNT electrode. The activated MWCNT-COOH polymer actuator surpassed the SWCNT actuator in terms of the strain and maximum Generated Stress.

  • Improved performance of an activated multi-walled carbon nanotube polymer actuator, compared with a single-walled carbon nanotube polymer actuator
    Sensors and Actuators B-chemical, 2012
    Co-Authors: Naohiro Terasawa, Ken Mukai, Kinji Asaka
    Abstract:

    Abstract Actuators were developed using activated multi-walled carbon nanotube (MWCNT)–ionic liquid (IL) gel electrodes, and were compared with non-activated MWCNT- and single-walled carbon nanotube (SWCNT)-based actuators, in terms of their electrochemical and electromechanical properties. Furthermore, the effects of variations in the IL on the electrochemical and electromechanical properties of the activated MWCNT–NH2/IL gel electrode actuators were investigated. The performance of the activated MWCNT–NH2 polymer actuator surpassed that of the SWCNT actuator, in terms of the strain and maximum Generated Stress. For the MWCNT–NH2 actuators, the strain and maximum Generated Stress were dependent on the IL species.

  • High performance polymer actuators based on multi-walled carbon nanotubes that surpass the performance of those containing single-walled carbon nanotubes: Effects of ionic liquid and composition
    Sensors and Actuators B: Chemical, 2012
    Co-Authors: Naohiro Terasawa, Norihiro Ono, Ken Mukai, Tomoyuki Koga, Nobuyuki Higashi, Kinji Asaka
    Abstract:

    The effects of ionic liquid (IL) and composition (ratio of carbon nanotube (CNT):polymer:IL) on the electrochemical and electromechanical properties of actuators containing activated and non-activated multi-walled carbon nanotube (MWCNT)-IL gel electrodes were investigated. The electrochemical and electromechanical properties of actuators containing the activated and non-activated MWCNT-IL gel electrodes were compared to those of a single-walled carbon nanotube (SWCNT)-based actuator. The double-layer capacitance of the activated MWCNT (MWCNT-COOH) electrode and the strain and maximum Generated Stress for the MWCNT-COOH or MWCNT actuator are dependent on both the IL and composition. For the MWCNT-COOH actuator containing 1-ethyl-3-methylimidazolium tetrafluoroborate (EMI[BF 4]) (MWCNT-COOH:poly(vinylidene fluoride-co-hexafluoropropylene):EMI[BF 4] = 30:30:35), the strain was 0.78-0.80% for the frequency range of 0.01-0.005 Hz, which was ca. twice that measured for the SWCNT actuator, and the maximum Generated Stress was ca. 1.8-2.5 times larger than that observed for the SWCNT actuator. Therefore, the common CNT activated MWCNT actuator can generate a maximum Stress sufficient for practical purposes without using specialized SWCNT. Furthermore, the actuator containing MWCNT-COOH performed better than those containing SWCNTs or MWCNTs, and gave a more rapid response. © 2011 Elsevier B.V. All rights reserved.

  • Improved performance of an activated multi-walled carbon nanotube polymer actuator, compared with a single-walled carbon nanotube polymer actuator
    Sensors and Actuators B: Chemical, 2012
    Co-Authors: Naohiro Terasawa, Ken Mukai, Kinji Asaka
    Abstract:

    Actuators were developed using activated multi-walled carbon nanotube (MWCNT)-ionic liquid (IL) gel electrodes, and were compared with non-activated MWCNT- and single-walled carbon nanotube (SWCNT)-based actuators, in terms of their electrochemical and electromechanical properties. Furthermore, the effects of variations in the IL on the electrochemical and electromechanical properties of the activated MWCNT-NH 2/IL gel electrode actuators were investigated. The performance of the activated MWCNT-NH 2 polymer actuator surpassed that of the SWCNT actuator, in terms of the strain and maximum Generated Stress. For the MWCNT-NH 2 actuators, the strain and maximum Generated Stress were dependent on the IL species. © 2012 Elsevier B.V.

  • Superior performance of non-activated multi-walled carbon nanotube polymer actuator containing ruthenium oxide over a single-walled carbon nanotube
    Carbon, 2012
    Co-Authors: Naohiro Terasawa, Ken Mukai, Kentaro Yamato, Kinji Asaka
    Abstract:

    The electrochemical and electromechanical properties of actuators developed using a non-activated multi-walled carbon nanotube (MWCNT)-ionic liquid (IL) gel electrode containing ruthenium oxide (RuO 2) were compared with only-MWCNT and only-single-walled carbon nanotube (SWCNT) based actuators. The double-layer capacitance of the non-activated MWCNT electrode containing RuO 2 was larger than that of the only-MWCNT electrode. The non-activated MWCNT polymer actuator containing RuO 2 surpassed the performance of the only-MWCNT and only-SWCNT actuators in terms of the strain and maximum Generated Stress. Both MWCNTs and RuO 2 were required to produce large strain and quick response actuators that surpassed the performance of the only-SWCNT polymer actuator and exhibited characteristics sufficient for practical applications (e.g. tactile display). © 2012 Elsevier Ltd. All rights reserved.

Hiromi Nakamura - One of the best experts on this subject based on the ideXlab platform.

  • glycosaminoglycan degradation reduces mineralized tissue titanium interfacial strength
    Journal of Biomedical Materials Research Part A, 2006
    Co-Authors: Hiromi Nakamura, Hideki Aita, Frank Butz, Jaewoo Shim, Vijay Gupta, Takahiro Ogawa
    Abstract:

    Although the localization of the proteoglycan/glycosaminoglycan (GAG) complex at the bone–titanium implant interface has been implied, the role of proteoglycans on the establishment of bone–titanium integration is unknown. The hypothesis to be tested was that proteoglycans play an important role in establishing bone–titanium interfacial adhesion. The objective of this study is to investigate the effect of proteoglycan knockdown by GAG enzymatic degradation on the interfacial strength between mineralized tissue and titanium having different surface topographies. Rat bone marrow-derived osteoblastic cells were cultured on either a machined titanium disk or an acid-etched titanium disk. At day 21 of culture, one of the three following GAG degradation enzymes was added into the culture; chondroitinase AC, chondroitinase B, or keratanase. After 3 days of incubation (at day 24 of culture), the laser spallation technique was applied to the samples in order to assess the tissue–titanium interfacial strength. In this technique, a laser-Generated Stress wave is used to separate the tissue–titanium interface, and the interfacial strength is determined interferometrically by recording the transient free surface velocity of the tissue. Mineralized tissue cultured on the acid-etched titanium showed 20–30% higher tissue interfacial strength than that cultured on the machined titanium (p < 0.0001). For both the machined and acid-etched surface cultures, administration of the enzyme reduced the interfacial strength by 25–30% compared with the untreated control cultures (p < 0.0001). There were no differences in the effect among the three different enzymes tested. A nanoindentation study revealed that the enzyme treatment did not affect the elastic modulus of the mineralized tissue. Scanning electron microscopic and energy dispersive spectroscopic analyses revealed less post-spallation tissue remnant on the titanium substrates when treated with the enzymes. The tissue remnant was greater in amount on the acid-etched surface than on the machined surface. The results suggest that there exists not only mechanical interlocking but also biological interfacial adhesion between the mineralized tissue and titanium, in which the proteoglycan/GAG complex is involved. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res, 2006

  • Glycosaminoglycan degradation reduces mineralized tissue–titanium interfacial strength
    Journal of Biomedical Materials Research Part A, 2006
    Co-Authors: Hiromi Nakamura, Hideki Aita, Frank Butz, Jaewoo Shim, Vijay Gupta, Takahiro Ogawa
    Abstract:

    Although the localization of the proteoglycan/glycosaminoglycan (GAG) complex at the bone–titanium implant interface has been implied, the role of proteoglycans on the establishment of bone–titanium integration is unknown. The hypothesis to be tested was that proteoglycans play an important role in establishing bone–titanium interfacial adhesion. The objective of this study is to investigate the effect of proteoglycan knockdown by GAG enzymatic degradation on the interfacial strength between mineralized tissue and titanium having different surface topographies. Rat bone marrow-derived osteoblastic cells were cultured on either a machined titanium disk or an acid-etched titanium disk. At day 21 of culture, one of the three following GAG degradation enzymes was added into the culture; chondroitinase AC, chondroitinase B, or keratanase. After 3 days of incubation (at day 24 of culture), the laser spallation technique was applied to the samples in order to assess the tissue–titanium interfacial strength. In this technique, a laser-Generated Stress wave is used to separate the tissue–titanium interface, and the interfacial strength is determined interferometrically by recording the transient free surface velocity of the tissue. Mineralized tissue cultured on the acid-etched titanium showed 20–30% higher tissue interfacial strength than that cultured on the machined titanium (p < 0.0001). For both the machined and acid-etched surface cultures, administration of the enzyme reduced the interfacial strength by 25–30% compared with the untreated control cultures (p < 0.0001). There were no differences in the effect among the three different enzymes tested. A nanoindentation study revealed that the enzyme treatment did not affect the elastic modulus of the mineralized tissue. Scanning electron microscopic and energy dispersive spectroscopic analyses revealed less post-spallation tissue remnant on the titanium substrates when treated with the enzymes. The tissue remnant was greater in amount on the acid-etched surface than on the machined surface. The results suggest that there exists not only mechanical interlocking but also biological interfacial adhesion between the mineralized tissue and titanium, in which the proteoglycan/GAG complex is involved. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res, 2006