The Experts below are selected from a list of 11841 Experts worldwide ranked by ideXlab platform

Xiangjin Meng - One of the best experts on this subject based on the ideXlab platform.

  • Genetic Identification and characterization of a novel virus related to human hepatitis e virus from chickens with hepatitis splenomegaly syndrome in the united states
    Journal of General Virology, 2001
    Co-Authors: Gholamreza Haqshenas, H L Shivaprasad, Peter R Woolcock, Deryck H Read, Xiangjin Meng
    Abstract:

    Hepatitis–splenomegaly (HS) syndrome is an emerging disease in chickens in North America; the cause of this disease is unknown. In this study, the Genetic Identification and characterization of a novel virus related to human hepatitis E virus (HEV) isolated from bile samples of chickens with HS syndrome is reported. Based upon the similar genomic organization and significant sequence identity of this virus with HEV, the virus has been tentatively named avian HEV in order to distinguish it from human and swine HEV. Electron microscopy revealed that avian HEV is a non-enveloped virus particle of 30–35 nm in diameter. The sequence of the 3′ half of the viral genome (∼4 kb) was determined. Sequence analyses revealed that this genomic region contains the complete 3′ non-coding region, the complete genes from open reading frames (ORFs) 2 and 3, the complete RNA-dependent RNA polymerase (RdRp) gene and a partial helicase gene from ORF 1. The helicase gene is the most conserved gene between avian HEV and other HEV strains, displaying 58–61% aa and 57–60% nt sequence identities. The RdRp gene of avian HEV shares 47–50% aa and 52–53% nt sequence identities and the putative capsid gene (ORF 2) of avian HEV shares 48–49% aa and 48–51% nt sequence identities with the corresponding regions of other known HEV strains. PhyloGenetic analysis indicates that avian HEV is Genetically related to, but distinct from, other known HEV strains. This discovery has important implications for HEV animal models, nomenclature and natural history.

  • Genetic Identification and characterization of a novel virus related to human hepatitis E virus from chickens with hepatitis–splenomegaly syndrome in the United States
    Journal of General Virology, 2001
    Co-Authors: Gholamreza Haqshenas, H L Shivaprasad, Peter R Woolcock, Deryck H Read, Xiangjin Meng
    Abstract:

    Hepatitis–splenomegaly (HS) syndrome is an emerging disease in chickens in North America; the cause of this disease is unknown. In this study, the Genetic Identification and characterization of a novel virus related to human hepatitis E virus (HEV) isolated from bile samples of chickens with HS syndrome is reported. Based upon the similar genomic organization and significant sequence identity of this virus with HEV, the virus has been tentatively named avian HEV in order to distinguish it from human and swine HEV. Electron microscopy revealed that avian HEV is a non-enveloped virus particle of 30–35 nm in diameter. The sequence of the 3′ half of the viral genome (∼4 kb) was determined. Sequence analyses revealed that this genomic region contains the complete 3′ non-coding region, the complete genes from open reading frames (ORFs) 2 and 3, the complete RNA-dependent RNA polymerase (RdRp) gene and a partial helicase gene from ORF 1. The helicase gene is the most conserved gene between avian HEV and other HEV strains, displaying 58–61% aa and 57–60% nt sequence identities. The RdRp gene of avian HEV shares 47–50% aa and 52–53% nt sequence identities and the putative capsid gene (ORF 2) of avian HEV shares 48–49% aa and 48–51% nt sequence identities with the corresponding regions of other known HEV strains. PhyloGenetic analysis indicates that avian HEV is Genetically related to, but distinct from, other known HEV strains. This discovery has important implications for HEV animal models, nomenclature and natural history.

Marian M. De Pancorbo - One of the best experts on this subject based on the ideXlab platform.

  • Updating data on the Genetic Identification of bone remains of victims of the Spanish Civil War
    Forensic Science International: Genetics Supplement Series, 2019
    Co-Authors: Miriam Baeta, Carolina Núñez, Caterina Raffone, Eva Granizo, Leire Palencia-madrid, Sergio Cardoso, Francisco Etxeberria, Lourdes Herrasti, Marian M. De Pancorbo
    Abstract:

    Abstract In this study, we present our experience in the Genetic Identification of skeletal remains recovered from graves of the Spanish Civil War (1936-1939) and posterior dictatorship (until 1970s). Up to now, we have carried out the Genetic analysis of more than 500 human remains from graves located in the Spanish territory, particularly from the northern half. Autosomal STRs, Y-STRs, X-STRs and/or mtDNA were studied in order to establish kinship relationship with presumptive relatives and reach a successful Identification of the unknown remains. In the last years, our efforts have focused on overcoming the limitations of this kind of analysis: limited quality and quantity of DNA recovered from the remains, partial Genetic profiles from these samples and scarce number of appropriate family members for Genetic comparisons. With this aim, we have optimized the process from the DNA extraction to the matching search, including an increased number of relatives’ profiles in the Genetic databases (n>800), in order to raise the number of Identifications.

  • A grave in my garden. Genetic Identification of Spanish civil war victims buried in two mass graves in Espinosa de los Monteros (Burgos, Spain)
    Forensic Science International: Genetics Supplement Series, 2015
    Co-Authors: Carolina Núñez, Miriam Baeta, Leire Palencia-madrid, Francisco Etxeberria, Lourdes Herrasti, Marian M. De Pancorbo
    Abstract:

    Abstract We report the Genetic study of 13 victims of the Spanish Civil war buried in two mass graves in Espinosa de los Monteros (Burgos, Spain). The grave with the highest number of individuals (grave 1; N =9) was found in the garden of a private house of the village. The other grave (grave 2) was only 200m away and the remains of four women were unexpectedly found. Skeletal remains, mainly teeth, were better preserved in graves 1 than 2. Mean DNA extraction yield was 0.17ng/μl. In order to carry out the Genetic Identification of the remains, buccal swabs from 11 family relatives were available. The choice of Genetic markers analyzed relied on the different levels of biological kinship to be elucidated. For 75% of the post-mortem samples, ≥12 autosomal STR profiles were obtained. Y chromosome STRs, as well as mitochondrial DNA control region, were also analyzed in order to study paternal and maternal lineages, respectively. Besides, X chromosome STRs were typed in one case to verify a paternal grandmother–granddaughter relationship. We successfully identified 9 of 13 individuals buried in the two mass graves of Espinosa de los Monteros, 6 in grave 1 and 3 in grave 2. The search of further relatives' samples would be of interest to perform more comparative analyses, and eventually do not leave any of the victims without a name.

  • Digging up the recent Spanish memory: Genetic Identification of human remains from mass graves of the Spanish Civil War and posterior dictatorship
    Forensic Science International-genetics, 2015
    Co-Authors: Miriam Baeta, Carolina Núñez, Leire Palencia-madrid, Sergio Cardoso, Francisco Etxeberria, Lourdes Herrasti, Marian M. De Pancorbo
    Abstract:

    Abstract The Spanish Civil War (1936–1939) and posterior dictatorship (until 1970s) stands as one of the major conflicts in the recent history of Spain. It led to nearly two hundred thousand men and women executed or murdered extra-judicially or after dubious legal procedures. Nowadays, most of them remain unidentified or even buried in irretraceable mass graves across Spain. Here, we present the Genetic Identification of human remains found in 26 mass graves located in Northern Spain. A total of 252 post-mortem remains were analyzed and compared to 186 relatives, allowing the Identification of 87 victims. Overall, a significant success of DNA profiling was reached, since informative profiles (≥12 STRs and/or mitochondrial DNA profile) were obtained in 85.71% of the remains. This high performance in DNA profiling from challenging samples demonstrated the efficacy of DNA extraction and amplification methods used herein, given that only around 14.29% of the samples did not provide an informative Genetic profile for the analysis performed, probably due to the presence of degraded and/or limited DNA in these remains. However, this study shows a partial Identification success rate, which is clearly a consequence of the lack of both appropriate family members for Genetic comparisons and accurate information about the victims’ location. Hence, further perseverance in the exhumation of other intact graves as well as in the search of more alleged relatives is crucial in order to facilitate and increase the number of Genetic Identifications.

Gholamreza Haqshenas - One of the best experts on this subject based on the ideXlab platform.

  • Genetic Identification and characterization of a novel virus related to human hepatitis E virus from chickens with hepatitis-splenomegaly syndrome in the United States.
    The Journal of general virology, 2020
    Co-Authors: Gholamreza Haqshenas, H L Shivaprasad, Peter R Woolcock, Deryck H Read, X J Meng
    Abstract:

    Hepatitis-splenomegaly (HS) syndrome is an emerging disease in chickens in North America; the cause of this disease is unknown. In this study, the Genetic Identification and characterization of a novel virus related to human hepatitis E virus (HEV) isolated from bile samples of chickens with HS syndrome is reported. Based upon the similar genomic organization and significant sequence identity of this virus with HEV, the virus has been tentatively named avian HEV in order to distinguish it from human and swine HEV. Electron microscopy revealed that avian HEV is a non-enveloped virus particle of 30-35 nm in diameter. The sequence of the 3' half of the viral genome ( approximately 4 kb) was determined. Sequence analyses revealed that this genomic region contains the complete 3' non-coding region, the complete genes from open reading frames (ORFs) 2 and 3, the complete RNA-dependent RNA polymerase (RdRp) gene and a partial helicase gene from ORF 1. The helicase gene is the most conserved gene between avian HEV and other HEV strains, displaying 58-61% aa and 57-60% nt sequence identities. The RdRp gene of avian HEV shares 47-50% aa and 52-53% nt sequence identities and the putative capsid gene (ORF 2) of avian HEV shares 48-49% aa and 48-51% nt sequence identities with the corresponding regions of other known HEV strains. PhyloGenetic analysis indicates that avian HEV is Genetically related to, but distinct from, other known HEV strains. This discovery has important implications for HEV animal models, nomenclature and natural history.

  • Genetic Identification and characterization of a novel virus related to human hepatitis e virus from chickens with hepatitis splenomegaly syndrome in the united states
    Journal of General Virology, 2001
    Co-Authors: Gholamreza Haqshenas, H L Shivaprasad, Peter R Woolcock, Deryck H Read, Xiangjin Meng
    Abstract:

    Hepatitis–splenomegaly (HS) syndrome is an emerging disease in chickens in North America; the cause of this disease is unknown. In this study, the Genetic Identification and characterization of a novel virus related to human hepatitis E virus (HEV) isolated from bile samples of chickens with HS syndrome is reported. Based upon the similar genomic organization and significant sequence identity of this virus with HEV, the virus has been tentatively named avian HEV in order to distinguish it from human and swine HEV. Electron microscopy revealed that avian HEV is a non-enveloped virus particle of 30–35 nm in diameter. The sequence of the 3′ half of the viral genome (∼4 kb) was determined. Sequence analyses revealed that this genomic region contains the complete 3′ non-coding region, the complete genes from open reading frames (ORFs) 2 and 3, the complete RNA-dependent RNA polymerase (RdRp) gene and a partial helicase gene from ORF 1. The helicase gene is the most conserved gene between avian HEV and other HEV strains, displaying 58–61% aa and 57–60% nt sequence identities. The RdRp gene of avian HEV shares 47–50% aa and 52–53% nt sequence identities and the putative capsid gene (ORF 2) of avian HEV shares 48–49% aa and 48–51% nt sequence identities with the corresponding regions of other known HEV strains. PhyloGenetic analysis indicates that avian HEV is Genetically related to, but distinct from, other known HEV strains. This discovery has important implications for HEV animal models, nomenclature and natural history.

  • Genetic Identification and characterization of a novel virus related to human hepatitis E virus from chickens with hepatitis–splenomegaly syndrome in the United States
    Journal of General Virology, 2001
    Co-Authors: Gholamreza Haqshenas, H L Shivaprasad, Peter R Woolcock, Deryck H Read, Xiangjin Meng
    Abstract:

    Hepatitis–splenomegaly (HS) syndrome is an emerging disease in chickens in North America; the cause of this disease is unknown. In this study, the Genetic Identification and characterization of a novel virus related to human hepatitis E virus (HEV) isolated from bile samples of chickens with HS syndrome is reported. Based upon the similar genomic organization and significant sequence identity of this virus with HEV, the virus has been tentatively named avian HEV in order to distinguish it from human and swine HEV. Electron microscopy revealed that avian HEV is a non-enveloped virus particle of 30–35 nm in diameter. The sequence of the 3′ half of the viral genome (∼4 kb) was determined. Sequence analyses revealed that this genomic region contains the complete 3′ non-coding region, the complete genes from open reading frames (ORFs) 2 and 3, the complete RNA-dependent RNA polymerase (RdRp) gene and a partial helicase gene from ORF 1. The helicase gene is the most conserved gene between avian HEV and other HEV strains, displaying 58–61% aa and 57–60% nt sequence identities. The RdRp gene of avian HEV shares 47–50% aa and 52–53% nt sequence identities and the putative capsid gene (ORF 2) of avian HEV shares 48–49% aa and 48–51% nt sequence identities with the corresponding regions of other known HEV strains. PhyloGenetic analysis indicates that avian HEV is Genetically related to, but distinct from, other known HEV strains. This discovery has important implications for HEV animal models, nomenclature and natural history.

H L Shivaprasad - One of the best experts on this subject based on the ideXlab platform.

  • Genetic Identification and characterization of a novel virus related to human hepatitis E virus from chickens with hepatitis-splenomegaly syndrome in the United States.
    The Journal of general virology, 2020
    Co-Authors: Gholamreza Haqshenas, H L Shivaprasad, Peter R Woolcock, Deryck H Read, X J Meng
    Abstract:

    Hepatitis-splenomegaly (HS) syndrome is an emerging disease in chickens in North America; the cause of this disease is unknown. In this study, the Genetic Identification and characterization of a novel virus related to human hepatitis E virus (HEV) isolated from bile samples of chickens with HS syndrome is reported. Based upon the similar genomic organization and significant sequence identity of this virus with HEV, the virus has been tentatively named avian HEV in order to distinguish it from human and swine HEV. Electron microscopy revealed that avian HEV is a non-enveloped virus particle of 30-35 nm in diameter. The sequence of the 3' half of the viral genome ( approximately 4 kb) was determined. Sequence analyses revealed that this genomic region contains the complete 3' non-coding region, the complete genes from open reading frames (ORFs) 2 and 3, the complete RNA-dependent RNA polymerase (RdRp) gene and a partial helicase gene from ORF 1. The helicase gene is the most conserved gene between avian HEV and other HEV strains, displaying 58-61% aa and 57-60% nt sequence identities. The RdRp gene of avian HEV shares 47-50% aa and 52-53% nt sequence identities and the putative capsid gene (ORF 2) of avian HEV shares 48-49% aa and 48-51% nt sequence identities with the corresponding regions of other known HEV strains. PhyloGenetic analysis indicates that avian HEV is Genetically related to, but distinct from, other known HEV strains. This discovery has important implications for HEV animal models, nomenclature and natural history.

  • Genetic Identification and characterization of a novel virus related to human hepatitis e virus from chickens with hepatitis splenomegaly syndrome in the united states
    Journal of General Virology, 2001
    Co-Authors: Gholamreza Haqshenas, H L Shivaprasad, Peter R Woolcock, Deryck H Read, Xiangjin Meng
    Abstract:

    Hepatitis–splenomegaly (HS) syndrome is an emerging disease in chickens in North America; the cause of this disease is unknown. In this study, the Genetic Identification and characterization of a novel virus related to human hepatitis E virus (HEV) isolated from bile samples of chickens with HS syndrome is reported. Based upon the similar genomic organization and significant sequence identity of this virus with HEV, the virus has been tentatively named avian HEV in order to distinguish it from human and swine HEV. Electron microscopy revealed that avian HEV is a non-enveloped virus particle of 30–35 nm in diameter. The sequence of the 3′ half of the viral genome (∼4 kb) was determined. Sequence analyses revealed that this genomic region contains the complete 3′ non-coding region, the complete genes from open reading frames (ORFs) 2 and 3, the complete RNA-dependent RNA polymerase (RdRp) gene and a partial helicase gene from ORF 1. The helicase gene is the most conserved gene between avian HEV and other HEV strains, displaying 58–61% aa and 57–60% nt sequence identities. The RdRp gene of avian HEV shares 47–50% aa and 52–53% nt sequence identities and the putative capsid gene (ORF 2) of avian HEV shares 48–49% aa and 48–51% nt sequence identities with the corresponding regions of other known HEV strains. PhyloGenetic analysis indicates that avian HEV is Genetically related to, but distinct from, other known HEV strains. This discovery has important implications for HEV animal models, nomenclature and natural history.

  • Genetic Identification and characterization of a novel virus related to human hepatitis E virus from chickens with hepatitis–splenomegaly syndrome in the United States
    Journal of General Virology, 2001
    Co-Authors: Gholamreza Haqshenas, H L Shivaprasad, Peter R Woolcock, Deryck H Read, Xiangjin Meng
    Abstract:

    Hepatitis–splenomegaly (HS) syndrome is an emerging disease in chickens in North America; the cause of this disease is unknown. In this study, the Genetic Identification and characterization of a novel virus related to human hepatitis E virus (HEV) isolated from bile samples of chickens with HS syndrome is reported. Based upon the similar genomic organization and significant sequence identity of this virus with HEV, the virus has been tentatively named avian HEV in order to distinguish it from human and swine HEV. Electron microscopy revealed that avian HEV is a non-enveloped virus particle of 30–35 nm in diameter. The sequence of the 3′ half of the viral genome (∼4 kb) was determined. Sequence analyses revealed that this genomic region contains the complete 3′ non-coding region, the complete genes from open reading frames (ORFs) 2 and 3, the complete RNA-dependent RNA polymerase (RdRp) gene and a partial helicase gene from ORF 1. The helicase gene is the most conserved gene between avian HEV and other HEV strains, displaying 58–61% aa and 57–60% nt sequence identities. The RdRp gene of avian HEV shares 47–50% aa and 52–53% nt sequence identities and the putative capsid gene (ORF 2) of avian HEV shares 48–49% aa and 48–51% nt sequence identities with the corresponding regions of other known HEV strains. PhyloGenetic analysis indicates that avian HEV is Genetically related to, but distinct from, other known HEV strains. This discovery has important implications for HEV animal models, nomenclature and natural history.

Peter R Woolcock - One of the best experts on this subject based on the ideXlab platform.

  • Genetic Identification and characterization of a novel virus related to human hepatitis E virus from chickens with hepatitis-splenomegaly syndrome in the United States.
    The Journal of general virology, 2020
    Co-Authors: Gholamreza Haqshenas, H L Shivaprasad, Peter R Woolcock, Deryck H Read, X J Meng
    Abstract:

    Hepatitis-splenomegaly (HS) syndrome is an emerging disease in chickens in North America; the cause of this disease is unknown. In this study, the Genetic Identification and characterization of a novel virus related to human hepatitis E virus (HEV) isolated from bile samples of chickens with HS syndrome is reported. Based upon the similar genomic organization and significant sequence identity of this virus with HEV, the virus has been tentatively named avian HEV in order to distinguish it from human and swine HEV. Electron microscopy revealed that avian HEV is a non-enveloped virus particle of 30-35 nm in diameter. The sequence of the 3' half of the viral genome ( approximately 4 kb) was determined. Sequence analyses revealed that this genomic region contains the complete 3' non-coding region, the complete genes from open reading frames (ORFs) 2 and 3, the complete RNA-dependent RNA polymerase (RdRp) gene and a partial helicase gene from ORF 1. The helicase gene is the most conserved gene between avian HEV and other HEV strains, displaying 58-61% aa and 57-60% nt sequence identities. The RdRp gene of avian HEV shares 47-50% aa and 52-53% nt sequence identities and the putative capsid gene (ORF 2) of avian HEV shares 48-49% aa and 48-51% nt sequence identities with the corresponding regions of other known HEV strains. PhyloGenetic analysis indicates that avian HEV is Genetically related to, but distinct from, other known HEV strains. This discovery has important implications for HEV animal models, nomenclature and natural history.

  • Genetic Identification and characterization of a novel virus related to human hepatitis e virus from chickens with hepatitis splenomegaly syndrome in the united states
    Journal of General Virology, 2001
    Co-Authors: Gholamreza Haqshenas, H L Shivaprasad, Peter R Woolcock, Deryck H Read, Xiangjin Meng
    Abstract:

    Hepatitis–splenomegaly (HS) syndrome is an emerging disease in chickens in North America; the cause of this disease is unknown. In this study, the Genetic Identification and characterization of a novel virus related to human hepatitis E virus (HEV) isolated from bile samples of chickens with HS syndrome is reported. Based upon the similar genomic organization and significant sequence identity of this virus with HEV, the virus has been tentatively named avian HEV in order to distinguish it from human and swine HEV. Electron microscopy revealed that avian HEV is a non-enveloped virus particle of 30–35 nm in diameter. The sequence of the 3′ half of the viral genome (∼4 kb) was determined. Sequence analyses revealed that this genomic region contains the complete 3′ non-coding region, the complete genes from open reading frames (ORFs) 2 and 3, the complete RNA-dependent RNA polymerase (RdRp) gene and a partial helicase gene from ORF 1. The helicase gene is the most conserved gene between avian HEV and other HEV strains, displaying 58–61% aa and 57–60% nt sequence identities. The RdRp gene of avian HEV shares 47–50% aa and 52–53% nt sequence identities and the putative capsid gene (ORF 2) of avian HEV shares 48–49% aa and 48–51% nt sequence identities with the corresponding regions of other known HEV strains. PhyloGenetic analysis indicates that avian HEV is Genetically related to, but distinct from, other known HEV strains. This discovery has important implications for HEV animal models, nomenclature and natural history.

  • Genetic Identification and characterization of a novel virus related to human hepatitis E virus from chickens with hepatitis–splenomegaly syndrome in the United States
    Journal of General Virology, 2001
    Co-Authors: Gholamreza Haqshenas, H L Shivaprasad, Peter R Woolcock, Deryck H Read, Xiangjin Meng
    Abstract:

    Hepatitis–splenomegaly (HS) syndrome is an emerging disease in chickens in North America; the cause of this disease is unknown. In this study, the Genetic Identification and characterization of a novel virus related to human hepatitis E virus (HEV) isolated from bile samples of chickens with HS syndrome is reported. Based upon the similar genomic organization and significant sequence identity of this virus with HEV, the virus has been tentatively named avian HEV in order to distinguish it from human and swine HEV. Electron microscopy revealed that avian HEV is a non-enveloped virus particle of 30–35 nm in diameter. The sequence of the 3′ half of the viral genome (∼4 kb) was determined. Sequence analyses revealed that this genomic region contains the complete 3′ non-coding region, the complete genes from open reading frames (ORFs) 2 and 3, the complete RNA-dependent RNA polymerase (RdRp) gene and a partial helicase gene from ORF 1. The helicase gene is the most conserved gene between avian HEV and other HEV strains, displaying 58–61% aa and 57–60% nt sequence identities. The RdRp gene of avian HEV shares 47–50% aa and 52–53% nt sequence identities and the putative capsid gene (ORF 2) of avian HEV shares 48–49% aa and 48–51% nt sequence identities with the corresponding regions of other known HEV strains. PhyloGenetic analysis indicates that avian HEV is Genetically related to, but distinct from, other known HEV strains. This discovery has important implications for HEV animal models, nomenclature and natural history.