The Experts below are selected from a list of 312 Experts worldwide ranked by ideXlab platform
Yoko Shimazu - One of the best experts on this subject based on the ideXlab platform.
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persistence of host specific bacteroides prevotella 16s rrna Genetic Markers in environmental waters effects of temperature and salinity
Applied Microbiology and Biotechnology, 2007Co-Authors: Satoshi Okabe, Yoko ShimazuAbstract:Host-specific Bacteroides–Prevotella 16S rRNA Genetic Markers are promising alternative indicators for identifying the sources of fecal pollution because of their high abundance in the feces of warm-blooded animals and high host specificity. However, little is known about the persistence of these Genetic Markers in environments after being released into environmental waters. The persistence of feces-derived four different host-specific Bacteroides–Prevotella 16S rRNA Genetic makers (total, human-, cow-, and pig-specific) in environmental waters was therefore investigated at different incubation temperatures (4, 10, 20, and 30°C) and salinities (0, 10, 20, and 30 ppt) and then compared with the survival of conventional fecal-indicator organisms. The host-specific Genetic Markers were monitored by using real-time polymerase chain reaction (PCR) assays with specific primer sets. Each host-specific Genetic marker showed similar responses in non-filtered river water and seawater: They persisted longer at lower temperatures and higher salinities. In addition, these Markers did not increase in all conditions tested. Decay rates for indicator organisms were lower than those for host-specific Genetic Markers at temperature above 10°C. Furthermore, we investigated whether the PCR-detectable 16S rRNA Genetic Markers reflect the presence of live target cells or dead target cells in environmental waters. The result revealed that the detection of the Bacteroides–Prevotella 16S rRNA Genetic Markers in environmental waters mainly reflected the presence of ‘viable but non-culturable’ Bacteroides–Prevotella cells. These findings indicate that seasonal and geographical variations in persistence of these host-specific Bacteroides–Prevotella 16S rRNA Genetic Markers must be considered when we use them as alternative fecal indicators in environmental waters.
Satoshi Okabe - One of the best experts on this subject based on the ideXlab platform.
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Effects of temperature and predator on the persistence of host-specific Bacteroides-Prevotella Genetic Markers in water.
Water Science and Technology, 2013Co-Authors: Ayano Kobayashi, Daisuke Sano, Satoshi OkabeAbstract:Genetic Markers derived from Bacteroidales spp. have been proposed as promising indicators for fecal contamination in the water environment. However, little is known about the persistency of Bacteroidales spp. 16S rRNA Genetic Markers in the natural environment, which hampers the precise identification of fecal contamination sources. In this study, the persistency of human-specific Bacteroidales spp. Genetic Markers in river water was investigated during a 3-week agitation. The copy number of Bacteroidales spp. Genetic marker was decreased with agitation time, and was very sensitive to water temperature. After the 3-week agitation, three clones of 18S rRNA gene related to Glaucoma scintillans , Spumella- like flagellate, and Colpidium campylum were acquired. The presence of predators that can prey on target bacteria could also be a critical factor affecting the quantified value of Genetic Markers. It is very important to take these factors, water temperature and the presence of predator, into account for predicting the fate of Genetic Markers to accurately identify fecal pollution sources.
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persistence of host specific bacteroides prevotella 16s rrna Genetic Markers in environmental waters effects of temperature and salinity
Applied Microbiology and Biotechnology, 2007Co-Authors: Satoshi Okabe, Yoko ShimazuAbstract:Host-specific Bacteroides–Prevotella 16S rRNA Genetic Markers are promising alternative indicators for identifying the sources of fecal pollution because of their high abundance in the feces of warm-blooded animals and high host specificity. However, little is known about the persistence of these Genetic Markers in environments after being released into environmental waters. The persistence of feces-derived four different host-specific Bacteroides–Prevotella 16S rRNA Genetic makers (total, human-, cow-, and pig-specific) in environmental waters was therefore investigated at different incubation temperatures (4, 10, 20, and 30°C) and salinities (0, 10, 20, and 30 ppt) and then compared with the survival of conventional fecal-indicator organisms. The host-specific Genetic Markers were monitored by using real-time polymerase chain reaction (PCR) assays with specific primer sets. Each host-specific Genetic marker showed similar responses in non-filtered river water and seawater: They persisted longer at lower temperatures and higher salinities. In addition, these Markers did not increase in all conditions tested. Decay rates for indicator organisms were lower than those for host-specific Genetic Markers at temperature above 10°C. Furthermore, we investigated whether the PCR-detectable 16S rRNA Genetic Markers reflect the presence of live target cells or dead target cells in environmental waters. The result revealed that the detection of the Bacteroides–Prevotella 16S rRNA Genetic Markers in environmental waters mainly reflected the presence of ‘viable but non-culturable’ Bacteroides–Prevotella cells. These findings indicate that seasonal and geographical variations in persistence of these host-specific Bacteroides–Prevotella 16S rRNA Genetic Markers must be considered when we use them as alternative fecal indicators in environmental waters.
Kristen C Ruegg - One of the best experts on this subject based on the ideXlab platform.
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mapping migration in a songbird using high resolution Genetic Markers
Molecular Ecology, 2014Co-Authors: Kristen C Ruegg, Eric C Anderson, Kristina L Paxton, Vanessa Apkenas, Sirena Lao, Rodney B Siegel, David F DesanteAbstract:Neotropic migratory birds are declining across the Western Hemisphere, but conservation efforts have been hampered by the inability to assess where migrants are most limited-the breeding grounds, migratory stopover sites or wintering areas. A major challenge has been the lack of an efficient, reliable and broadly applicable method for measuring the strength of migratory connections between populations across the annual cycle. Here, we show how high-resolution Genetic Markers can be used to identify Genetically distinct groups of a migratory bird, the Wilson's warbler (Cardellina pusilla), at fine enough spatial scales to facilitate assessing regional drivers of demographic trends. By screening 1626 samples using 96 highly divergent single nucleotide polymorphisms selected from a large pool of candidates (~450 000), we identify novel region-specific migratory routes and timetables of migration along the Pacific Flyway. Our results illustrate that high-resolution Genetic Markers are more reliable, precise and amenable to high throughput screening than previously described intrinsic marking techniques, making them broadly applicable to large-scale monitoring and conservation of migratory organisms.
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mapping migration in a songbird using high resolution Genetic Markers
bioRxiv, 2014Co-Authors: Kristen C Ruegg, Eric C Anderson, Kristina L Paxton, Vanessa Apkenas, Sirena Lao, Rodney B Siegel, David F Desante, Frank R Moore, Thomas B SmithAbstract:Neotropical migratory birds are declining across the Western Hemisphere, but conservation efforts have been hampered by the inability to assess where migrants are most limited–the breeding grounds, migratory stopover sites, or wintering areas. A major challenge has been the lack of an efficient, reliable, and broadly applicable method for connecting populations across the annual cycle. Here we show how high-resolution Genetic Markers can be used to identify populations of a migratory bird, the Wilson's warbler (Cardellina pusilla), at fine enough spatial scales to facilitate assessing regional drivers of demographic trends. By screening 1626 samples using 96 single nucleotide polymorphisms (SNPs) selected from a large pool of candidates (~450,000), we identify novel region-specific migratory routes and timetables of migration along the Pacific Flyway. Our results illustrate that high-resolution Genetic Markers are more reliable, accurate, and amenable to high throughput screening than previously described tracking techniques, making them broadly applicable to large-scale monitoring and conservation of migratory organisms.
David F Desante - One of the best experts on this subject based on the ideXlab platform.
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mapping migration in a songbird using high resolution Genetic Markers
Molecular Ecology, 2014Co-Authors: Kristen C Ruegg, Eric C Anderson, Kristina L Paxton, Vanessa Apkenas, Sirena Lao, Rodney B Siegel, David F DesanteAbstract:Neotropic migratory birds are declining across the Western Hemisphere, but conservation efforts have been hampered by the inability to assess where migrants are most limited-the breeding grounds, migratory stopover sites or wintering areas. A major challenge has been the lack of an efficient, reliable and broadly applicable method for measuring the strength of migratory connections between populations across the annual cycle. Here, we show how high-resolution Genetic Markers can be used to identify Genetically distinct groups of a migratory bird, the Wilson's warbler (Cardellina pusilla), at fine enough spatial scales to facilitate assessing regional drivers of demographic trends. By screening 1626 samples using 96 highly divergent single nucleotide polymorphisms selected from a large pool of candidates (~450 000), we identify novel region-specific migratory routes and timetables of migration along the Pacific Flyway. Our results illustrate that high-resolution Genetic Markers are more reliable, precise and amenable to high throughput screening than previously described intrinsic marking techniques, making them broadly applicable to large-scale monitoring and conservation of migratory organisms.
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mapping migration in a songbird using high resolution Genetic Markers
bioRxiv, 2014Co-Authors: Kristen C Ruegg, Eric C Anderson, Kristina L Paxton, Vanessa Apkenas, Sirena Lao, Rodney B Siegel, David F Desante, Frank R Moore, Thomas B SmithAbstract:Neotropical migratory birds are declining across the Western Hemisphere, but conservation efforts have been hampered by the inability to assess where migrants are most limited–the breeding grounds, migratory stopover sites, or wintering areas. A major challenge has been the lack of an efficient, reliable, and broadly applicable method for connecting populations across the annual cycle. Here we show how high-resolution Genetic Markers can be used to identify populations of a migratory bird, the Wilson's warbler (Cardellina pusilla), at fine enough spatial scales to facilitate assessing regional drivers of demographic trends. By screening 1626 samples using 96 single nucleotide polymorphisms (SNPs) selected from a large pool of candidates (~450,000), we identify novel region-specific migratory routes and timetables of migration along the Pacific Flyway. Our results illustrate that high-resolution Genetic Markers are more reliable, accurate, and amenable to high throughput screening than previously described tracking techniques, making them broadly applicable to large-scale monitoring and conservation of migratory organisms.
Eric C Anderson - One of the best experts on this subject based on the ideXlab platform.
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mapping migration in a songbird using high resolution Genetic Markers
Molecular Ecology, 2014Co-Authors: Kristen C Ruegg, Eric C Anderson, Kristina L Paxton, Vanessa Apkenas, Sirena Lao, Rodney B Siegel, David F DesanteAbstract:Neotropic migratory birds are declining across the Western Hemisphere, but conservation efforts have been hampered by the inability to assess where migrants are most limited-the breeding grounds, migratory stopover sites or wintering areas. A major challenge has been the lack of an efficient, reliable and broadly applicable method for measuring the strength of migratory connections between populations across the annual cycle. Here, we show how high-resolution Genetic Markers can be used to identify Genetically distinct groups of a migratory bird, the Wilson's warbler (Cardellina pusilla), at fine enough spatial scales to facilitate assessing regional drivers of demographic trends. By screening 1626 samples using 96 highly divergent single nucleotide polymorphisms selected from a large pool of candidates (~450 000), we identify novel region-specific migratory routes and timetables of migration along the Pacific Flyway. Our results illustrate that high-resolution Genetic Markers are more reliable, precise and amenable to high throughput screening than previously described intrinsic marking techniques, making them broadly applicable to large-scale monitoring and conservation of migratory organisms.
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mapping migration in a songbird using high resolution Genetic Markers
bioRxiv, 2014Co-Authors: Kristen C Ruegg, Eric C Anderson, Kristina L Paxton, Vanessa Apkenas, Sirena Lao, Rodney B Siegel, David F Desante, Frank R Moore, Thomas B SmithAbstract:Neotropical migratory birds are declining across the Western Hemisphere, but conservation efforts have been hampered by the inability to assess where migrants are most limited–the breeding grounds, migratory stopover sites, or wintering areas. A major challenge has been the lack of an efficient, reliable, and broadly applicable method for connecting populations across the annual cycle. Here we show how high-resolution Genetic Markers can be used to identify populations of a migratory bird, the Wilson's warbler (Cardellina pusilla), at fine enough spatial scales to facilitate assessing regional drivers of demographic trends. By screening 1626 samples using 96 single nucleotide polymorphisms (SNPs) selected from a large pool of candidates (~450,000), we identify novel region-specific migratory routes and timetables of migration along the Pacific Flyway. Our results illustrate that high-resolution Genetic Markers are more reliable, accurate, and amenable to high throughput screening than previously described tracking techniques, making them broadly applicable to large-scale monitoring and conservation of migratory organisms.
