The Experts below are selected from a list of 398904 Experts worldwide ranked by ideXlab platform

Pablo Bielza - One of the best experts on this subject based on the ideXlab platform.

  • Genetic study of mediterranean and south american populations of tomato leafminer tuta absoluta povolny 1994 lepidoptera gelechiidae using ribosomal and mitochondrial markers
    Pest Management Science, 2011
    Co-Authors: Dina Cifuentes, Robert Chynoweth, Pablo Bielza
    Abstract:

    BACKGROUND: Before its introduction into Europe at the end of 2006, Tuta absoluta (Povolny, 1994) was confined solely to South America. Currently, this invasive pest is well established in various European and Mediterranean countries, causing important economic losses to tomato (Lycopersicon esculentum Mill.) crops. In order to study the Genetic variability of this pest, 23 Mediterranean and ten native South American populations were analysed with nuclear ribosomal DNA (rDNA) and mitochondrial DNA (mtDNA) markers. RESULTS: The internal transcribed spacers 1 (ITS1) and 2 (ITS2) of rDNA and a fragment in the mtDNA gene encoding cytochrome oxidase I (COI) were PCR amplified and sequenced in T. absoluta. Sequence analyses consistently revealed neither intrapopulation nor interpopulation variation in either genomic region. CONCLUSIONS: High Genetic homogeneity was detected in T. absoluta populations from the Mediterranean Basin and South America, based on mtCOI and ITS rDNA sequence analysis. A single Genetic Type was identified in this pest.

  • Genetic study of mediterranean and south american populations of tomato leafminer tuta absoluta povolny 1994 lepidoptera gelechiidae using ribosomal and mitochondrial markers
    Pest Management Science, 2011
    Co-Authors: Dina Cifuentes, Robert Chynoweth, Pablo Bielza
    Abstract:

    BACKGROUND: Before its introduction into Europe at the end of 2006, Tuta absoluta (Povolny, 1994) was confined solely to South America. Currently, this invasive pest is well established in various European and Mediterranean countries, causing important economic losses to tomato (Lycopersicon esculentum Mill.) crops. In order to study the Genetic variability of this pest, 23 Mediterranean and ten native South American populations were analysed with nuclear ribosomal DNA (rDNA) and mitochondrial DNA (mtDNA) markers. RESULTS: The internal transcribed spacers 1 (ITS1) and 2 (ITS2) of rDNA and a fragment in the mtDNA gene encoding cytochrome oxidase I (COI) were PCR amplified and sequenced in T. absoluta. Sequence analyses consistently revealed neither intrapopulation nor interpopulation variation in either genomic region. CONCLUSIONS: High Genetic homogeneity was detected in T. absoluta populations from the Mediterranean Basin and South America, based on mtCOI and ITS rDNA sequence analysis. A single Genetic Type was identified in this pest. Copyright © 2011 Society of Chemical Industry

Dina Cifuentes - One of the best experts on this subject based on the ideXlab platform.

  • Genetic study of mediterranean and south american populations of tomato leafminer tuta absoluta povolny 1994 lepidoptera gelechiidae using ribosomal and mitochondrial markers
    Pest Management Science, 2011
    Co-Authors: Dina Cifuentes, Robert Chynoweth, Pablo Bielza
    Abstract:

    BACKGROUND: Before its introduction into Europe at the end of 2006, Tuta absoluta (Povolny, 1994) was confined solely to South America. Currently, this invasive pest is well established in various European and Mediterranean countries, causing important economic losses to tomato (Lycopersicon esculentum Mill.) crops. In order to study the Genetic variability of this pest, 23 Mediterranean and ten native South American populations were analysed with nuclear ribosomal DNA (rDNA) and mitochondrial DNA (mtDNA) markers. RESULTS: The internal transcribed spacers 1 (ITS1) and 2 (ITS2) of rDNA and a fragment in the mtDNA gene encoding cytochrome oxidase I (COI) were PCR amplified and sequenced in T. absoluta. Sequence analyses consistently revealed neither intrapopulation nor interpopulation variation in either genomic region. CONCLUSIONS: High Genetic homogeneity was detected in T. absoluta populations from the Mediterranean Basin and South America, based on mtCOI and ITS rDNA sequence analysis. A single Genetic Type was identified in this pest.

  • Genetic study of mediterranean and south american populations of tomato leafminer tuta absoluta povolny 1994 lepidoptera gelechiidae using ribosomal and mitochondrial markers
    Pest Management Science, 2011
    Co-Authors: Dina Cifuentes, Robert Chynoweth, Pablo Bielza
    Abstract:

    BACKGROUND: Before its introduction into Europe at the end of 2006, Tuta absoluta (Povolny, 1994) was confined solely to South America. Currently, this invasive pest is well established in various European and Mediterranean countries, causing important economic losses to tomato (Lycopersicon esculentum Mill.) crops. In order to study the Genetic variability of this pest, 23 Mediterranean and ten native South American populations were analysed with nuclear ribosomal DNA (rDNA) and mitochondrial DNA (mtDNA) markers. RESULTS: The internal transcribed spacers 1 (ITS1) and 2 (ITS2) of rDNA and a fragment in the mtDNA gene encoding cytochrome oxidase I (COI) were PCR amplified and sequenced in T. absoluta. Sequence analyses consistently revealed neither intrapopulation nor interpopulation variation in either genomic region. CONCLUSIONS: High Genetic homogeneity was detected in T. absoluta populations from the Mediterranean Basin and South America, based on mtCOI and ITS rDNA sequence analysis. A single Genetic Type was identified in this pest. Copyright © 2011 Society of Chemical Industry

Robert Chynoweth - One of the best experts on this subject based on the ideXlab platform.

  • Genetic study of mediterranean and south american populations of tomato leafminer tuta absoluta povolny 1994 lepidoptera gelechiidae using ribosomal and mitochondrial markers
    Pest Management Science, 2011
    Co-Authors: Dina Cifuentes, Robert Chynoweth, Pablo Bielza
    Abstract:

    BACKGROUND: Before its introduction into Europe at the end of 2006, Tuta absoluta (Povolny, 1994) was confined solely to South America. Currently, this invasive pest is well established in various European and Mediterranean countries, causing important economic losses to tomato (Lycopersicon esculentum Mill.) crops. In order to study the Genetic variability of this pest, 23 Mediterranean and ten native South American populations were analysed with nuclear ribosomal DNA (rDNA) and mitochondrial DNA (mtDNA) markers. RESULTS: The internal transcribed spacers 1 (ITS1) and 2 (ITS2) of rDNA and a fragment in the mtDNA gene encoding cytochrome oxidase I (COI) were PCR amplified and sequenced in T. absoluta. Sequence analyses consistently revealed neither intrapopulation nor interpopulation variation in either genomic region. CONCLUSIONS: High Genetic homogeneity was detected in T. absoluta populations from the Mediterranean Basin and South America, based on mtCOI and ITS rDNA sequence analysis. A single Genetic Type was identified in this pest.

  • Genetic study of mediterranean and south american populations of tomato leafminer tuta absoluta povolny 1994 lepidoptera gelechiidae using ribosomal and mitochondrial markers
    Pest Management Science, 2011
    Co-Authors: Dina Cifuentes, Robert Chynoweth, Pablo Bielza
    Abstract:

    BACKGROUND: Before its introduction into Europe at the end of 2006, Tuta absoluta (Povolny, 1994) was confined solely to South America. Currently, this invasive pest is well established in various European and Mediterranean countries, causing important economic losses to tomato (Lycopersicon esculentum Mill.) crops. In order to study the Genetic variability of this pest, 23 Mediterranean and ten native South American populations were analysed with nuclear ribosomal DNA (rDNA) and mitochondrial DNA (mtDNA) markers. RESULTS: The internal transcribed spacers 1 (ITS1) and 2 (ITS2) of rDNA and a fragment in the mtDNA gene encoding cytochrome oxidase I (COI) were PCR amplified and sequenced in T. absoluta. Sequence analyses consistently revealed neither intrapopulation nor interpopulation variation in either genomic region. CONCLUSIONS: High Genetic homogeneity was detected in T. absoluta populations from the Mediterranean Basin and South America, based on mtCOI and ITS rDNA sequence analysis. A single Genetic Type was identified in this pest. Copyright © 2011 Society of Chemical Industry

Michel Peterschmitt - One of the best experts on this subject based on the ideXlab platform.

  • a new silverleaf inducing bioType ms of bemisia tabaci hemiptera aleyrodidae indigenous to the islands of the south west indian ocean
    Bulletin of Entomological Research, 2005
    Co-Authors: Helene Delatte, Bernard Reynaud, Martine Granier, Laetitia Thornary, Jeanmichel Lett, Rob Goldbach, Michel Peterschmitt
    Abstract:

    Following the first detection of tomato yellow leaf curl virus (TYLCV) from Reunion (700 km east of Madagascar) in 1997 and the upsurge of Bemisia tabaci (Gennadius) on vegetable crops, two Genetic Types of B. tabaci were distinguished using RAPD?PCR and cytochrome oxidase I (COI) gene sequence comparisons. One Type was assigned to bioType B and the other was Genetically dissimilar to the populations described elsewhere and was named Ms, after the Mascarenes Archipelago. This new Genetic Type forms a distinct group that is sister to two other groups, one to which the B bioType is a member and one to which the Q bioType belongs. The Ms bioType is thought to be indigenous to the region as it was also detected in Mauritius, the Seychelles and Madagascar. Both B and Ms populations of B. tabaci induced silverleaf symptoms on Cucurbita sp., and were able to acquire and transmit TYLCV. Taken together these results indicate that the Ms Genetic Type should be considered a new bioType of B. tabaci

  • a new silverleaf inducing bioType ms of bemisia tabaci hemiptera aleyrodidae indigenous to the islands of the south west indian ocean
    Bulletin of Entomological Research, 2005
    Co-Authors: Helene Delatte, Bernard Reynaud, Martine Granier, Laetitia Thornary, Jeanmichel Lett, Rob Goldbach, Michel Peterschmitt
    Abstract:

    Following the first detection of tomato yellow leaf curl virus (TYLCV) from Reunion (700 km east of Madagascar) in 1997 and the upsurge of Bemisia tabaci (Gennadius) on vegetable crops, two Genetic Types of B. tabaci were distinguished using RAPT-PCR and cytochrome oxidase I (COI) gene sequence comparisons. One Type was assigned to bioType B and the other was Genetically dissimilar to the populations described elsewhere and was named Ms, after the Mascarenes Archipelago. This new Genetic Type forms a distinct group that is sister to two other groups, one to which the B bioType is a member and one to which the Q bioType belongs. The Ms bioType is thought to be indigenous to the region as it was also detected in Mauritius, the Seychelles and Madagascar. Both B and Ms populations of B. tabaci induced silverleaf symptoms on Cucurbita sp., and were able to acquire and transmit TYLCV. Taken together these results indicate that the Ms Genetic Type should be considered a new bioType of B. tabaci. (Resume d'auteur)

Christian Diot - One of the best experts on this subject based on the ideXlab platform.

  • RNA-seq analysis of hepatic gene expression of common Pekin, Muscovy, mule and hinny ducks fed ad libitum or overfed
    BMC Genomics, 2019
    Co-Authors: Frédéric Herault, Elisabeth Baeza, Nathalie Houee-bigot, Olivier Bouchez, Diane Esquerré, Christophe Klopp, Christian Diot
    Abstract:

    Duck species are known to have different susceptibility to fatty liver production in response to overfeeding. In order to better describe mechanisms involved in the development of hepatic steatosis and differences between species, transcriptome analyses were conducted on RNAs extracted from the livers of Pekin and Muscovy duck species and of their reciprocal hybrids, Mule and Hinny ducks fed ad libitum or overfed to identify differentially expressed genes and associated functions. After extraction from the liver of ducks from the four Genetic Types, RNAs were sequenced and sequencing data were analyzed. Hierarchic clustering and principal component analyses of genes expression levels indicated that differences between individuals lie primarily in feeding effect, differences between Genetic Types being less important. However, Muscovy ducks fed ad libitum and overfed were clustered together. Interestingly, Hinny and Mule hybrid ducks could not be differentiated from each other, according to feeding. Many genes with expression differences between overfed and ad libitum fed ducks were identified in each Genetic Type. Functional annotation analyses of these differentially expressed genes highlighted some expected functions (carbohydrate and lipid metabolisms) but also some unexpected ones (cell proliferation and immunity). These analyses evidence differences in response to overfeeding between different Genetic Types and help to better characterize functions involved in hepatic steatosis in ducks.

  • RNA-seq analysis of hepatic gene expression of common Pekin, Muscovy, mule and hinny ducks fed ad libitum or overfed
    BMC, 2019
    Co-Authors: Frédéric Herault, Elisabeth Baeza, Olivier Bouchez, Diane Esquerré, Christophe Klopp, Magalie Houée-bigot, Christian Diot
    Abstract:

    Abstract Background Duck species are known to have different susceptibility to fatty liver production in response to overfeeding. In order to better describe mechanisms involved in the development of hepatic steatosis and differences between species, transcriptome analyses were conducted on RNAs extracted from the livers of Pekin and Muscovy duck species and of their reciprocal hybrids, Mule and Hinny ducks fed ad libitum or overfed to identify differentially expressed genes and associated functions. Results After extraction from the liver of ducks from the four Genetic Types, RNAs were sequenced and sequencing data were analyzed. Hierarchic clustering and principal component analyses of genes expression levels indicated that differences between individuals lie primarily in feeding effect, differences between Genetic Types being less important. However, Muscovy ducks fed ad libitum and overfed were clustered together. Interestingly, Hinny and Mule hybrid ducks could not be differentiated from each other, according to feeding. Many genes with expression differences between overfed and ad libitum fed ducks were identified in each Genetic Type. Functional annotation analyses of these differentially expressed genes highlighted some expected functions (carbohydrate and lipid metabolisms) but also some unexpected ones (cell proliferation and immunity). Conclusions These analyses evidence differences in response to overfeeding between different Genetic Types and help to better characterize functions involved in hepatic steatosis in ducks