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I Stockley - One of the best experts on this subject based on the ideXlab platform.
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comparison of the elution properties of commercially available Gentamicin and vancomycin impregnated bone cement with home made preparations
Orthopaedic Proceedings, 2018Co-Authors: N Frew, Tim Nichol, Thomas J Smith, I StockleyAbstract:IntroductionVancomycin is commonly added to acrylic bone cement during revision arthroplasty surgery. Proprietary cement preparations containing vancomycin are available but significantly more expensive. We investigated whether the antibiotic elution and mechanical strength of ‘home-made’ vancomycin containing bone cement was comparable to commercial vancomycin-impregnated cement.MethodsA total of 18 cement discs of constant size, containing either proprietary CopalG+V®; or ‘home-made’ CopalR+G® with vancomycin added by hand, were made. Each disc contained the same antibiotic quantities (0.5g gentamycin, 2g vancomycin) and was immersed in ammonium acetate buffer in a sealed container. Fluid from each container was sampled at eight time points over a two week period. The concentration of Gentamicin and vancomycin in the fluid was analysed using high performance liquid chromatography mass spectrometry. The impact strength of each PMMA cement preparation was measured using a Charpy-type impact tester.Results...
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comparison of the elution properties of commercially available Gentamicin and bone cement containing vancomycin with home made preparations
Journal of Bone and Joint Surgery-british Volume, 2017Co-Authors: N Frew, T Cannon, Tim Nichol, Thomas J Smith, I StockleyAbstract:Aims Vancomycin is commonly added to acrylic bone cement during revision arthroplasty surgery. Proprietary cement preparations containing vancomycin are available, but are significantly more expensive. We investigated whether the elution of antibiotic from ‘home-made’ cement containing vancomycin was comparable with more expensive commercially available vancomycin impregnated cement. Materials and Methods A total of 18 cement discs containing either proprietary CopalG+V; or ‘home-made’ CopalR+G with vancomycin added by hand, were made. Each disc contained the same amount of antibiotic (0.5 g gentamycin, 2 g vancomycin) and was immersed in ammonium acetate buffer in a sealed container. Fluid from each container was sampled at eight time points over a two-week period. The concentrations of Gentamicin and vancomycin in the fluid were analysed using high performance liquid chromatography mass spectrometry. Results The highest peak concentrations of antibiotic were observed from the ‘home-made’ cements containing vancomycin, added as in the operating theatre. The overall elution of antibiotic was, fivefold (vancomycin) and twofold (Gentamicin) greater from the ‘home-made’ mix compared with the commercially mixed cement. The use of a vacuum during mixing had no significant effect on antibiotic elution in any of the samples. Conclusion These findings suggest that the addition of 2 g vancomycin powder to Gentamicin-impregnated bone cement by hand significantly increases the elution of both antibiotics compared with commercially prepared cements containing vancomycin. We found no significant advantages of using expensive commercially produced vancomycin-impregnated cement and recommend the addition of vancomycin powder by hand in the operating theatre. Cite this article: Bone Joint J 2017;99-B:73–7.
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comparison of the elution properties of commercially available Gentamicin and vancomycin impregnated bone cement with home made preparations
Journal of Bone and Joint Surgery-british Volume, 2016Co-Authors: N Frew, Tim Nichol, Thomas J Smith, I StockleyAbstract:Introduction Vancomycin is commonly added to acrylic bone cement during revision arthroplasty surgery. Proprietary cement preparations containing vancomycin are available but significantly more expensive. We investigated whether the antibiotic elution and mechanical strength of ‘home-made’ vancomycin containing bone cement was comparable to commercial vancomycin-impregnated cement. Methods A total of 18 cement discs of constant size, containing either proprietary CopalG+V ® ; or ‘home-made’ CopalR+G ® with vancomycin added by hand, were made. Each disc contained the same antibiotic quantities (0.5g gentamycin, 2g vancomycin) and was immersed in ammonium acetate buffer in a sealed container. Fluid from each container was sampled at eight time points over a two week period. The concentration of Gentamicin and vancomycin in the fluid was analysed using high performance liquid chromatography mass spectrometry. The impact strength of each PMMA cement preparation was measured using a Charpy-type impact tester. Results Highest peak antibiotic concentrations were observed from the ‘home-made’ vancomycin containing cement, added as in the operating theatre. Overall antibiotic elution was, five-fold (vancomycin) and two-fold (Gentamicin), greater from the ‘home-made’ mix compared to commercially mixed cement. However the ‘home-made’ cements showed greater variation in elution kinetics compared to the commercial mix. Use of a vacuum during mixing had no significant effect on antibiotic elution in any of the samples. Impact strength testing showed no significant differences between the groups. Discussion Our findings suggest the addition of 2g vancomycin powder to Gentamicin-impregnated bone cement in theatre, significantly increases elution of both antibiotics, with no significant loss of strength, compared to commercially prepared cement. Conclusion We have found no significant advantages of expensive off-the-shelf vancomycin-impregnated bone cement and recommend the addition of vancomycin powder by hand when making cement beads and spacers.
Martin F Desimone - One of the best experts on this subject based on the ideXlab platform.
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antibiotic loaded silica nanoparticle collagen composite hydrogels with prolonged antimicrobial activity for wound infection prevention
Journal of Materials Chemistry B, 2014Co-Authors: Gisela Solange Alvarez, Christophe Helary, Andrea Mathilde Mebert, Xiaolin Wang, Thibaud Coradin, Martin F DesimoneAbstract:Silica–collagen type I nanocomposite hydrogels are evaluated as medicated dressings to prevent infection in chronic wounds. Two antibiotics, Gentamicin and rifamycin, are encapsulated in a single step within plain silica nanoparticles. Their antimicrobial efficiency against Pseudomonas aeruginosa and Staphylococcus aureus is assessed. Gentamycin-loaded 500 nm particles can be immobilized at high silica dose in concentrated collagen hydrogels without modifying their fibrillar structure or impacting on their rheological behavior and increases their proteolytic stability. Gentamicin release from the nanocomposites is sustained over 7 days, offering an unparalleled prolonged antibacterial activity. Particle immobilization also decreases their cytotoxicity towards surface-seeded fibroblast cells. Rifamycin-loaded 100 nm particles significantly alter the collagen hydrogel structure at high silica doses. The thus-obtained nanocomposites show no antibacterial efficiency, due to strong adsorption of rifamycin on collagen fibers. The complex interplay of interactions between drugs, silica and collagen is a key factor regulating the properties of these composite hydrogels as antibiotic-delivering biological dressings and must be taken into account for future extension to other wound healing agents.
Thibaud Coradin - One of the best experts on this subject based on the ideXlab platform.
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antibiotic loaded silica nanoparticle collagen composite hydrogels with prolonged antimicrobial activity for wound infection prevention
Journal of Materials Chemistry B, 2014Co-Authors: Gisela Solange Alvarez, Christophe Helary, Andrea Mathilde Mebert, Xiaolin Wang, Thibaud Coradin, Martin F DesimoneAbstract:Silica–collagen type I nanocomposite hydrogels are evaluated as medicated dressings to prevent infection in chronic wounds. Two antibiotics, Gentamicin and rifamycin, are encapsulated in a single step within plain silica nanoparticles. Their antimicrobial efficiency against Pseudomonas aeruginosa and Staphylococcus aureus is assessed. Gentamycin-loaded 500 nm particles can be immobilized at high silica dose in concentrated collagen hydrogels without modifying their fibrillar structure or impacting on their rheological behavior and increases their proteolytic stability. Gentamicin release from the nanocomposites is sustained over 7 days, offering an unparalleled prolonged antibacterial activity. Particle immobilization also decreases their cytotoxicity towards surface-seeded fibroblast cells. Rifamycin-loaded 100 nm particles significantly alter the collagen hydrogel structure at high silica doses. The thus-obtained nanocomposites show no antibacterial efficiency, due to strong adsorption of rifamycin on collagen fibers. The complex interplay of interactions between drugs, silica and collagen is a key factor regulating the properties of these composite hydrogels as antibiotic-delivering biological dressings and must be taken into account for future extension to other wound healing agents.
Xiaolin Wang - One of the best experts on this subject based on the ideXlab platform.
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antibiotic loaded silica nanoparticle collagen composite hydrogels with prolonged antimicrobial activity for wound infection prevention
Journal of Materials Chemistry B, 2014Co-Authors: Gisela Solange Alvarez, Christophe Helary, Andrea Mathilde Mebert, Xiaolin Wang, Thibaud Coradin, Martin F DesimoneAbstract:Silica–collagen type I nanocomposite hydrogels are evaluated as medicated dressings to prevent infection in chronic wounds. Two antibiotics, Gentamicin and rifamycin, are encapsulated in a single step within plain silica nanoparticles. Their antimicrobial efficiency against Pseudomonas aeruginosa and Staphylococcus aureus is assessed. Gentamycin-loaded 500 nm particles can be immobilized at high silica dose in concentrated collagen hydrogels without modifying their fibrillar structure or impacting on their rheological behavior and increases their proteolytic stability. Gentamicin release from the nanocomposites is sustained over 7 days, offering an unparalleled prolonged antibacterial activity. Particle immobilization also decreases their cytotoxicity towards surface-seeded fibroblast cells. Rifamycin-loaded 100 nm particles significantly alter the collagen hydrogel structure at high silica doses. The thus-obtained nanocomposites show no antibacterial efficiency, due to strong adsorption of rifamycin on collagen fibers. The complex interplay of interactions between drugs, silica and collagen is a key factor regulating the properties of these composite hydrogels as antibiotic-delivering biological dressings and must be taken into account for future extension to other wound healing agents.
Christophe Helary - One of the best experts on this subject based on the ideXlab platform.
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antibiotic loaded silica nanoparticle collagen composite hydrogels with prolonged antimicrobial activity for wound infection prevention
Journal of Materials Chemistry B, 2014Co-Authors: Gisela Solange Alvarez, Christophe Helary, Andrea Mathilde Mebert, Xiaolin Wang, Thibaud Coradin, Martin F DesimoneAbstract:Silica–collagen type I nanocomposite hydrogels are evaluated as medicated dressings to prevent infection in chronic wounds. Two antibiotics, Gentamicin and rifamycin, are encapsulated in a single step within plain silica nanoparticles. Their antimicrobial efficiency against Pseudomonas aeruginosa and Staphylococcus aureus is assessed. Gentamycin-loaded 500 nm particles can be immobilized at high silica dose in concentrated collagen hydrogels without modifying their fibrillar structure or impacting on their rheological behavior and increases their proteolytic stability. Gentamicin release from the nanocomposites is sustained over 7 days, offering an unparalleled prolonged antibacterial activity. Particle immobilization also decreases their cytotoxicity towards surface-seeded fibroblast cells. Rifamycin-loaded 100 nm particles significantly alter the collagen hydrogel structure at high silica doses. The thus-obtained nanocomposites show no antibacterial efficiency, due to strong adsorption of rifamycin on collagen fibers. The complex interplay of interactions between drugs, silica and collagen is a key factor regulating the properties of these composite hydrogels as antibiotic-delivering biological dressings and must be taken into account for future extension to other wound healing agents.