The Experts below are selected from a list of 120 Experts worldwide ranked by ideXlab platform

Vladimir Mihailovic - One of the best experts on this subject based on the ideXlab platform.

  • comparative phytochemical analysis of gentiana cruciata l roots and aerial parts and their biological activities
    Industrial Crops and Products, 2015
    Co-Authors: Vladimir Mihailovic, Danijela Misic, Sanja Matic, Mirjana Mihailovic, Snežana Stanic, Miroslav M Vrvic, Jelena Katanic, Milan Mladenovic, Nevena Stankovic, Tatjana Boroja
    Abstract:

    Abstract The purpose of this study was to evaluate the antioxidant potential of methanol extracts of Gentiana cruciata L. aerial parts and roots, as well as the stability of the phenolic compounds and antioxidant capacity of extracts during heating, at different pHs and after an in vitro digestion procedure. Also, their genotoxicity and antigenotoxicity against carbon tetrachloride in the liver of albino Wistar rats using the comet assay were evaluated. Three secoiridoid glycosides (swertiamarin, Gentiopicrin, and sweroside) and four phenolic compounds (orientin, vitexin and two isovitexin-glucosides) were identified as the major constituents in aerial parts and roots of G. cruciata , using UHPLC-DAD/±HESI-MS/MS analysis. The results of antioxidant assays showed that aerial parts displayed higher antioxidant activity compared to the roots, which could be related to higher phenolics content, especially flavonoids. In general, extracts showed pH and thermal stability, while duodenal condition had more influence on total phenolic condition and antioxidant activity of extracts. Both extracts showed a protective effect against CCl 4 in comet assays. The roots extract showed no genotoxic activity, while aerial parts extract showed slight genotoxicity at concentrations of 400 mg/kg b.w.

  • hepatoprotective effects of secoiridoid rich extracts from gentiana cruciata l against carbon tetrachloride induced liver damage in rats
    Food & Function, 2014
    Co-Authors: Vladimir Mihailovic, Danijela Misic, Mirjana Mihailovic, Jelena Katanic, Milan Mladenovic, Aleksandra Uskokovic, Jelena Arambasic, Vesna Stankovic, Slavica Solujic, Nevena Stankovic
    Abstract:

    The objective of this work was to investigate the effects of the methanol extracts of Gentiana cruciata L. aerial parts (GCA) and roots (GCR) against carbon tetrachloride-induced liver injury in rats. Pretreatment with GCA and GCR, containing sweroside, swertiamarin and Gentiopicrin in high concentrations, dose-dependently and significantly decreased the levels of serum transaminases, alkaline phosphatase and total bilirubin, whereas an increase in the level of total protein was found compared with the CCl4-treated group. Moreover, oral administration of extracts significantly enhanced antioxidant enzyme activities (superoxide dismutase and catalase), increased the content of glutathione and decreased the content of TBARS. Microscopic evaluations of the liver revealed CCl4-induced lesions and related toxic manifestations that were minimal in the liver of rats pretreated with extracts at the dose of 400 mg per kg b.w. The results suggest that the use of G. cruciata extracts has a merit as a potent candidate in protecting the liver against chemical induced toxicity.

  • hepatoprotective effects of gentiana asclepiadea l extracts against carbon tetrachloride induced liver injury in rats
    Food and Chemical Toxicology, 2013
    Co-Authors: Vladimir Mihailovic, Danijela Misic, Mirjana Mihailovic, Jelena Katanic, Milan Mladenovic, Aleksandra Uskokovic, Jelena Arambasic, Vesna Stankovic, Slavica Solujic, Sanja Matic
    Abstract:

    Abstract This study is an attempt to evaluate the hepatoprotective activity of Gentiana asclepiadea L. against carbon tetrachloride-induced liver injury in rats. Methanol extracts of aerial parts (GAA) and roots (GAR) of G. asclepiadea at doses of 100, 200, and 400 mg/kg b.w. were orally administered to Wistar rats once daily for 7 days before they were treated with CCl4. The hepatoprotective activity of the extracts in this study was compared with the reference drug silymarin. In CCl4 treated animals, GAA and GAR significantly decreased levels of serum transaminases, alkaline phosphatase and total bilirubin, and increased the level of total protein. Treatment with the extracts resulted in a significant increase in the levels of catalase, superoxide dismutase and reduced glutathione, accompanied with a marked reduction in the levels of malondialdehyde, as compared to CCl4 treated group. The histopathological studies confirmed protective effects of extracts against CCl4-induced liver injuries. No genotoxicity was observed in liver cells after GAA treatment, while GAR showed only slight genotoxic effects by comet assay. Phytochemical analysis revealed the presence of sweroside, swertiamarin and Gentiopicrin in high concentrations in both extracts. It could be concluded that the use of G. asclepiadea extracts in the treatment of chemical-induced hepatotoxicity.

Milan Mladenovic - One of the best experts on this subject based on the ideXlab platform.

  • comparative phytochemical analysis of gentiana cruciata l roots and aerial parts and their biological activities
    Industrial Crops and Products, 2015
    Co-Authors: Vladimir Mihailovic, Danijela Misic, Sanja Matic, Mirjana Mihailovic, Snežana Stanic, Miroslav M Vrvic, Jelena Katanic, Milan Mladenovic, Nevena Stankovic, Tatjana Boroja
    Abstract:

    Abstract The purpose of this study was to evaluate the antioxidant potential of methanol extracts of Gentiana cruciata L. aerial parts and roots, as well as the stability of the phenolic compounds and antioxidant capacity of extracts during heating, at different pHs and after an in vitro digestion procedure. Also, their genotoxicity and antigenotoxicity against carbon tetrachloride in the liver of albino Wistar rats using the comet assay were evaluated. Three secoiridoid glycosides (swertiamarin, Gentiopicrin, and sweroside) and four phenolic compounds (orientin, vitexin and two isovitexin-glucosides) were identified as the major constituents in aerial parts and roots of G. cruciata , using UHPLC-DAD/±HESI-MS/MS analysis. The results of antioxidant assays showed that aerial parts displayed higher antioxidant activity compared to the roots, which could be related to higher phenolics content, especially flavonoids. In general, extracts showed pH and thermal stability, while duodenal condition had more influence on total phenolic condition and antioxidant activity of extracts. Both extracts showed a protective effect against CCl 4 in comet assays. The roots extract showed no genotoxic activity, while aerial parts extract showed slight genotoxicity at concentrations of 400 mg/kg b.w.

  • hepatoprotective effects of secoiridoid rich extracts from gentiana cruciata l against carbon tetrachloride induced liver damage in rats
    Food & Function, 2014
    Co-Authors: Vladimir Mihailovic, Danijela Misic, Mirjana Mihailovic, Jelena Katanic, Milan Mladenovic, Aleksandra Uskokovic, Jelena Arambasic, Vesna Stankovic, Slavica Solujic, Nevena Stankovic
    Abstract:

    The objective of this work was to investigate the effects of the methanol extracts of Gentiana cruciata L. aerial parts (GCA) and roots (GCR) against carbon tetrachloride-induced liver injury in rats. Pretreatment with GCA and GCR, containing sweroside, swertiamarin and Gentiopicrin in high concentrations, dose-dependently and significantly decreased the levels of serum transaminases, alkaline phosphatase and total bilirubin, whereas an increase in the level of total protein was found compared with the CCl4-treated group. Moreover, oral administration of extracts significantly enhanced antioxidant enzyme activities (superoxide dismutase and catalase), increased the content of glutathione and decreased the content of TBARS. Microscopic evaluations of the liver revealed CCl4-induced lesions and related toxic manifestations that were minimal in the liver of rats pretreated with extracts at the dose of 400 mg per kg b.w. The results suggest that the use of G. cruciata extracts has a merit as a potent candidate in protecting the liver against chemical induced toxicity.

  • hepatoprotective effects of gentiana asclepiadea l extracts against carbon tetrachloride induced liver injury in rats
    Food and Chemical Toxicology, 2013
    Co-Authors: Vladimir Mihailovic, Danijela Misic, Mirjana Mihailovic, Jelena Katanic, Milan Mladenovic, Aleksandra Uskokovic, Jelena Arambasic, Vesna Stankovic, Slavica Solujic, Sanja Matic
    Abstract:

    Abstract This study is an attempt to evaluate the hepatoprotective activity of Gentiana asclepiadea L. against carbon tetrachloride-induced liver injury in rats. Methanol extracts of aerial parts (GAA) and roots (GAR) of G. asclepiadea at doses of 100, 200, and 400 mg/kg b.w. were orally administered to Wistar rats once daily for 7 days before they were treated with CCl4. The hepatoprotective activity of the extracts in this study was compared with the reference drug silymarin. In CCl4 treated animals, GAA and GAR significantly decreased levels of serum transaminases, alkaline phosphatase and total bilirubin, and increased the level of total protein. Treatment with the extracts resulted in a significant increase in the levels of catalase, superoxide dismutase and reduced glutathione, accompanied with a marked reduction in the levels of malondialdehyde, as compared to CCl4 treated group. The histopathological studies confirmed protective effects of extracts against CCl4-induced liver injuries. No genotoxicity was observed in liver cells after GAA treatment, while GAR showed only slight genotoxic effects by comet assay. Phytochemical analysis revealed the presence of sweroside, swertiamarin and Gentiopicrin in high concentrations in both extracts. It could be concluded that the use of G. asclepiadea extracts in the treatment of chemical-induced hepatotoxicity.

Jelena Katanic - One of the best experts on this subject based on the ideXlab platform.

  • comparative phytochemical analysis of gentiana cruciata l roots and aerial parts and their biological activities
    Industrial Crops and Products, 2015
    Co-Authors: Vladimir Mihailovic, Danijela Misic, Sanja Matic, Mirjana Mihailovic, Snežana Stanic, Miroslav M Vrvic, Jelena Katanic, Milan Mladenovic, Nevena Stankovic, Tatjana Boroja
    Abstract:

    Abstract The purpose of this study was to evaluate the antioxidant potential of methanol extracts of Gentiana cruciata L. aerial parts and roots, as well as the stability of the phenolic compounds and antioxidant capacity of extracts during heating, at different pHs and after an in vitro digestion procedure. Also, their genotoxicity and antigenotoxicity against carbon tetrachloride in the liver of albino Wistar rats using the comet assay were evaluated. Three secoiridoid glycosides (swertiamarin, Gentiopicrin, and sweroside) and four phenolic compounds (orientin, vitexin and two isovitexin-glucosides) were identified as the major constituents in aerial parts and roots of G. cruciata , using UHPLC-DAD/±HESI-MS/MS analysis. The results of antioxidant assays showed that aerial parts displayed higher antioxidant activity compared to the roots, which could be related to higher phenolics content, especially flavonoids. In general, extracts showed pH and thermal stability, while duodenal condition had more influence on total phenolic condition and antioxidant activity of extracts. Both extracts showed a protective effect against CCl 4 in comet assays. The roots extract showed no genotoxic activity, while aerial parts extract showed slight genotoxicity at concentrations of 400 mg/kg b.w.

  • hepatoprotective effects of secoiridoid rich extracts from gentiana cruciata l against carbon tetrachloride induced liver damage in rats
    Food & Function, 2014
    Co-Authors: Vladimir Mihailovic, Danijela Misic, Mirjana Mihailovic, Jelena Katanic, Milan Mladenovic, Aleksandra Uskokovic, Jelena Arambasic, Vesna Stankovic, Slavica Solujic, Nevena Stankovic
    Abstract:

    The objective of this work was to investigate the effects of the methanol extracts of Gentiana cruciata L. aerial parts (GCA) and roots (GCR) against carbon tetrachloride-induced liver injury in rats. Pretreatment with GCA and GCR, containing sweroside, swertiamarin and Gentiopicrin in high concentrations, dose-dependently and significantly decreased the levels of serum transaminases, alkaline phosphatase and total bilirubin, whereas an increase in the level of total protein was found compared with the CCl4-treated group. Moreover, oral administration of extracts significantly enhanced antioxidant enzyme activities (superoxide dismutase and catalase), increased the content of glutathione and decreased the content of TBARS. Microscopic evaluations of the liver revealed CCl4-induced lesions and related toxic manifestations that were minimal in the liver of rats pretreated with extracts at the dose of 400 mg per kg b.w. The results suggest that the use of G. cruciata extracts has a merit as a potent candidate in protecting the liver against chemical induced toxicity.

  • hepatoprotective effects of gentiana asclepiadea l extracts against carbon tetrachloride induced liver injury in rats
    Food and Chemical Toxicology, 2013
    Co-Authors: Vladimir Mihailovic, Danijela Misic, Mirjana Mihailovic, Jelena Katanic, Milan Mladenovic, Aleksandra Uskokovic, Jelena Arambasic, Vesna Stankovic, Slavica Solujic, Sanja Matic
    Abstract:

    Abstract This study is an attempt to evaluate the hepatoprotective activity of Gentiana asclepiadea L. against carbon tetrachloride-induced liver injury in rats. Methanol extracts of aerial parts (GAA) and roots (GAR) of G. asclepiadea at doses of 100, 200, and 400 mg/kg b.w. were orally administered to Wistar rats once daily for 7 days before they were treated with CCl4. The hepatoprotective activity of the extracts in this study was compared with the reference drug silymarin. In CCl4 treated animals, GAA and GAR significantly decreased levels of serum transaminases, alkaline phosphatase and total bilirubin, and increased the level of total protein. Treatment with the extracts resulted in a significant increase in the levels of catalase, superoxide dismutase and reduced glutathione, accompanied with a marked reduction in the levels of malondialdehyde, as compared to CCl4 treated group. The histopathological studies confirmed protective effects of extracts against CCl4-induced liver injuries. No genotoxicity was observed in liver cells after GAA treatment, while GAR showed only slight genotoxic effects by comet assay. Phytochemical analysis revealed the presence of sweroside, swertiamarin and Gentiopicrin in high concentrations in both extracts. It could be concluded that the use of G. asclepiadea extracts in the treatment of chemical-induced hepatotoxicity.

Mirjana Mihailovic - One of the best experts on this subject based on the ideXlab platform.

  • comparative phytochemical analysis of gentiana cruciata l roots and aerial parts and their biological activities
    Industrial Crops and Products, 2015
    Co-Authors: Vladimir Mihailovic, Danijela Misic, Sanja Matic, Mirjana Mihailovic, Snežana Stanic, Miroslav M Vrvic, Jelena Katanic, Milan Mladenovic, Nevena Stankovic, Tatjana Boroja
    Abstract:

    Abstract The purpose of this study was to evaluate the antioxidant potential of methanol extracts of Gentiana cruciata L. aerial parts and roots, as well as the stability of the phenolic compounds and antioxidant capacity of extracts during heating, at different pHs and after an in vitro digestion procedure. Also, their genotoxicity and antigenotoxicity against carbon tetrachloride in the liver of albino Wistar rats using the comet assay were evaluated. Three secoiridoid glycosides (swertiamarin, Gentiopicrin, and sweroside) and four phenolic compounds (orientin, vitexin and two isovitexin-glucosides) were identified as the major constituents in aerial parts and roots of G. cruciata , using UHPLC-DAD/±HESI-MS/MS analysis. The results of antioxidant assays showed that aerial parts displayed higher antioxidant activity compared to the roots, which could be related to higher phenolics content, especially flavonoids. In general, extracts showed pH and thermal stability, while duodenal condition had more influence on total phenolic condition and antioxidant activity of extracts. Both extracts showed a protective effect against CCl 4 in comet assays. The roots extract showed no genotoxic activity, while aerial parts extract showed slight genotoxicity at concentrations of 400 mg/kg b.w.

  • hepatoprotective effects of secoiridoid rich extracts from gentiana cruciata l against carbon tetrachloride induced liver damage in rats
    Food & Function, 2014
    Co-Authors: Vladimir Mihailovic, Danijela Misic, Mirjana Mihailovic, Jelena Katanic, Milan Mladenovic, Aleksandra Uskokovic, Jelena Arambasic, Vesna Stankovic, Slavica Solujic, Nevena Stankovic
    Abstract:

    The objective of this work was to investigate the effects of the methanol extracts of Gentiana cruciata L. aerial parts (GCA) and roots (GCR) against carbon tetrachloride-induced liver injury in rats. Pretreatment with GCA and GCR, containing sweroside, swertiamarin and Gentiopicrin in high concentrations, dose-dependently and significantly decreased the levels of serum transaminases, alkaline phosphatase and total bilirubin, whereas an increase in the level of total protein was found compared with the CCl4-treated group. Moreover, oral administration of extracts significantly enhanced antioxidant enzyme activities (superoxide dismutase and catalase), increased the content of glutathione and decreased the content of TBARS. Microscopic evaluations of the liver revealed CCl4-induced lesions and related toxic manifestations that were minimal in the liver of rats pretreated with extracts at the dose of 400 mg per kg b.w. The results suggest that the use of G. cruciata extracts has a merit as a potent candidate in protecting the liver against chemical induced toxicity.

  • hepatoprotective effects of gentiana asclepiadea l extracts against carbon tetrachloride induced liver injury in rats
    Food and Chemical Toxicology, 2013
    Co-Authors: Vladimir Mihailovic, Danijela Misic, Mirjana Mihailovic, Jelena Katanic, Milan Mladenovic, Aleksandra Uskokovic, Jelena Arambasic, Vesna Stankovic, Slavica Solujic, Sanja Matic
    Abstract:

    Abstract This study is an attempt to evaluate the hepatoprotective activity of Gentiana asclepiadea L. against carbon tetrachloride-induced liver injury in rats. Methanol extracts of aerial parts (GAA) and roots (GAR) of G. asclepiadea at doses of 100, 200, and 400 mg/kg b.w. were orally administered to Wistar rats once daily for 7 days before they were treated with CCl4. The hepatoprotective activity of the extracts in this study was compared with the reference drug silymarin. In CCl4 treated animals, GAA and GAR significantly decreased levels of serum transaminases, alkaline phosphatase and total bilirubin, and increased the level of total protein. Treatment with the extracts resulted in a significant increase in the levels of catalase, superoxide dismutase and reduced glutathione, accompanied with a marked reduction in the levels of malondialdehyde, as compared to CCl4 treated group. The histopathological studies confirmed protective effects of extracts against CCl4-induced liver injuries. No genotoxicity was observed in liver cells after GAA treatment, while GAR showed only slight genotoxic effects by comet assay. Phytochemical analysis revealed the presence of sweroside, swertiamarin and Gentiopicrin in high concentrations in both extracts. It could be concluded that the use of G. asclepiadea extracts in the treatment of chemical-induced hepatotoxicity.

Danijela Misic - One of the best experts on this subject based on the ideXlab platform.

  • comparative phytochemical analysis of gentiana cruciata l roots and aerial parts and their biological activities
    Industrial Crops and Products, 2015
    Co-Authors: Vladimir Mihailovic, Danijela Misic, Sanja Matic, Mirjana Mihailovic, Snežana Stanic, Miroslav M Vrvic, Jelena Katanic, Milan Mladenovic, Nevena Stankovic, Tatjana Boroja
    Abstract:

    Abstract The purpose of this study was to evaluate the antioxidant potential of methanol extracts of Gentiana cruciata L. aerial parts and roots, as well as the stability of the phenolic compounds and antioxidant capacity of extracts during heating, at different pHs and after an in vitro digestion procedure. Also, their genotoxicity and antigenotoxicity against carbon tetrachloride in the liver of albino Wistar rats using the comet assay were evaluated. Three secoiridoid glycosides (swertiamarin, Gentiopicrin, and sweroside) and four phenolic compounds (orientin, vitexin and two isovitexin-glucosides) were identified as the major constituents in aerial parts and roots of G. cruciata , using UHPLC-DAD/±HESI-MS/MS analysis. The results of antioxidant assays showed that aerial parts displayed higher antioxidant activity compared to the roots, which could be related to higher phenolics content, especially flavonoids. In general, extracts showed pH and thermal stability, while duodenal condition had more influence on total phenolic condition and antioxidant activity of extracts. Both extracts showed a protective effect against CCl 4 in comet assays. The roots extract showed no genotoxic activity, while aerial parts extract showed slight genotoxicity at concentrations of 400 mg/kg b.w.

  • hepatoprotective effects of secoiridoid rich extracts from gentiana cruciata l against carbon tetrachloride induced liver damage in rats
    Food & Function, 2014
    Co-Authors: Vladimir Mihailovic, Danijela Misic, Mirjana Mihailovic, Jelena Katanic, Milan Mladenovic, Aleksandra Uskokovic, Jelena Arambasic, Vesna Stankovic, Slavica Solujic, Nevena Stankovic
    Abstract:

    The objective of this work was to investigate the effects of the methanol extracts of Gentiana cruciata L. aerial parts (GCA) and roots (GCR) against carbon tetrachloride-induced liver injury in rats. Pretreatment with GCA and GCR, containing sweroside, swertiamarin and Gentiopicrin in high concentrations, dose-dependently and significantly decreased the levels of serum transaminases, alkaline phosphatase and total bilirubin, whereas an increase in the level of total protein was found compared with the CCl4-treated group. Moreover, oral administration of extracts significantly enhanced antioxidant enzyme activities (superoxide dismutase and catalase), increased the content of glutathione and decreased the content of TBARS. Microscopic evaluations of the liver revealed CCl4-induced lesions and related toxic manifestations that were minimal in the liver of rats pretreated with extracts at the dose of 400 mg per kg b.w. The results suggest that the use of G. cruciata extracts has a merit as a potent candidate in protecting the liver against chemical induced toxicity.

  • hepatoprotective effects of gentiana asclepiadea l extracts against carbon tetrachloride induced liver injury in rats
    Food and Chemical Toxicology, 2013
    Co-Authors: Vladimir Mihailovic, Danijela Misic, Mirjana Mihailovic, Jelena Katanic, Milan Mladenovic, Aleksandra Uskokovic, Jelena Arambasic, Vesna Stankovic, Slavica Solujic, Sanja Matic
    Abstract:

    Abstract This study is an attempt to evaluate the hepatoprotective activity of Gentiana asclepiadea L. against carbon tetrachloride-induced liver injury in rats. Methanol extracts of aerial parts (GAA) and roots (GAR) of G. asclepiadea at doses of 100, 200, and 400 mg/kg b.w. were orally administered to Wistar rats once daily for 7 days before they were treated with CCl4. The hepatoprotective activity of the extracts in this study was compared with the reference drug silymarin. In CCl4 treated animals, GAA and GAR significantly decreased levels of serum transaminases, alkaline phosphatase and total bilirubin, and increased the level of total protein. Treatment with the extracts resulted in a significant increase in the levels of catalase, superoxide dismutase and reduced glutathione, accompanied with a marked reduction in the levels of malondialdehyde, as compared to CCl4 treated group. The histopathological studies confirmed protective effects of extracts against CCl4-induced liver injuries. No genotoxicity was observed in liver cells after GAA treatment, while GAR showed only slight genotoxic effects by comet assay. Phytochemical analysis revealed the presence of sweroside, swertiamarin and Gentiopicrin in high concentrations in both extracts. It could be concluded that the use of G. asclepiadea extracts in the treatment of chemical-induced hepatotoxicity.