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Paco Cárdenas - One of the best experts on this subject based on the ideXlab platform.
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the effects of sampling and storage conditions on the metabolite profile of the marine sponge Geodia barretti
Frontiers in Chemistry, 2021Co-Authors: Ida Erngren, Paco Cárdenas, Eva Smit, Curt Pettersson, Mikael HedelandAbstract:Geodia barretti is a marine sponge common in the north Atlantic and waters outside of Norway and Sweden. The sampling and subsequent treatment as well as storage of sponges for metabolomics analyse ...
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reproductive biology of Geodia species porifera tetractinellida from boreo arctic north atlantic deep sea sponge grounds
Frontiers in Marine Science, 2020Co-Authors: Hans Tore Rapp, Paco Cárdenas, Vasiliki Koutsouveli, Maria Conejero, Ana RiesgoAbstract:Reproductive biology of Geodia (Porifera, Tetractinellida) from boreo-arctic North-Atlantic deep-sea sponge grounds
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First records of Geodia demosponges from the New England seamounts, an opportunity to test the use of DNA mini-barcodes on museum specimens
Marine Biodiversity, 2019Co-Authors: Paco Cárdenas, Jon A. MooreAbstract:We report the first records of the sponge genus Geodia (Demospongiae, Tetractinellida, Geodiidae) from the New England Seamounts and Muir Seamount, at lower bathyal depths. Nine specimens collected between 2000 and 2005 belong to two boreal species ( Geodia macandrewii and Geodia barretti ) and a temperate species ( Geodia megastrella ). These records extend the distributions of these deep-sea amphi-Atlantic species to the west. Most of these specimens were originally fixed in formalin, which substantially degraded the DNA. We nonetheless managed to sequence two cytochrome c oxidase subunit I (COI) mini-barcodes: the universal mini-barcode at the 5′ end of the Folmer barcode (130 bp) and a newly proposed mini-barcode at the 3′ end of the Folmer barcode (296 bp). These mini-barcodes unambiguously confirmed our identifications. As an additional test, we also successfully sequenced these two mini-barcodes from the holotype of G. barretti , collected in 1855. We conclude by advocating the use of mini-barcodes on formalin-fixed or old specimens with degraded DNA.
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Geodia boreo-arctic distribution
2016Co-Authors: Paco Cárdenas, Hans Tore Rapp, Megan Best, Mikael Thollesson, Anne Birgitte Klitgaard, Ole Secher TendalAbstract:This excel file includes locality records for all six species of boreo-arctic Geodia examined by us in various campaigns/museums collections and from the litterature. This file includes geographical coordinates, museum collection or reference, temperature and salinity when available. When the latitude/longitude information was missing but the locality was given, we reconstructed the geographic coordinates using Google Earth. Temperature ranges for each species were obtained from the campaigns in which bottom temperatures were recorded (e.g. Ingolf Exp., BIOICE, BIOFAR, PA1994, PA2010-009) and from the literature
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List of Geodia specimens examined
2016Co-Authors: Paco Cárdenas, Hans Tore Rapp, Megan Best, Mikael Thollesson, Anne Birgitte Klitgaard, Ole Secher TendalAbstract:List of boreo-arctic Geodia specimens identified for this study and used to build the distribution maps: In cases where we have many samples from approximately the same locality only a minor number of records are listed; the number in parentheses is the number of specimens caught at each station
Werner E. G. Müller - One of the best experts on this subject based on the ideXlab platform.
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physicochemical and functional characterization of the polymerization process of the Geodia cydonium lectin
FEBS Journal, 2008Co-Authors: Barbel Diehlseifert, G Uhlenbruck, Rudolf K Zahn, Manfred Geisert, Werner E. G. MüllerAbstract:The extracellularly localized, galactose-specific lectin from the sponge Geodia cydonium binds at one class of sites, 40 mol Ca2+/mol lectin with an association constant (Ka) of 0.3 X 10(6)M-1. Stoichiometric calculations reveal that in the extracellular milieu 22 mol Ca2+ (maximum) are complexed per mol lectin. Binding of Ca2+ to the lectin increases its apparent Mr from 44000 to 56000 (electrophoretic determination) or from 36500 to 53500 (high-pressure liquid gel chromatographical determination); the s20, w increases from 4.3 S to 4.5 S if Ca2+ is added to the lectin. In the presence of Ca2+ the lectin undergoes a conformational change perhaps by expanding the carbohydrate side chains which are terminated by galactose. Subsequently the lectin molecules polymerize to large three-dimensional clumps (diameter up to 8 micron). Turbidimetric studies reveal an inhibition of the lectin polymerization by lactose. The Ka of the lectin-lectin polymerization rises from 0.9 X 10(6)M-1 to 14.0 X 10(6)M-1 after increasing the Ca2+ concentration (from 1 microM to 100 microM). Parallel with this increase in affinity, the Ka value of the lectin-aggregation factor binding drops from 41.2 X 10(6)M-1 (1 microM Ca2+) to 1.3 X 10(6)M-1 (100 microM Ca2+). In the absence of Ca2+, the Geodia lectin forms 1-10-micron two-dimensional sheets in the presence of homologous glycoconjugates. Cell binding experiments with polyacrylamide gels, containing covalently bound galactose, show that both homologous (Geodia cydonium) and heterologous cells (L5178y) bind with a higher affinity to the lectin-polymer matrix than to the lectin-monomer one. These data suggest that lectin-polymer structures, together with lectin-glycoconjugate associates, are components of the cell-substrate adhesion system(s) of sponges in vivo.
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initiation of an aquaculture of sponges for the sustainable production of bioactive metabolites in open systems example Geodia cydonium
Marine Biotechnology, 1999Co-Authors: Werner E. G. Müller, Renato Batel, Wolfram Wimmer, Wolfgang Schatton, Markus Bohm, Zelimir FilicAbstract:Among Metazoa, sponges (phylum Porifera) are the richest source for different bioactive compounds. The availability of the raw material is, however, restricted. To obtain enough of the bioactive compounds for application in human therapy, sponges have to be cultured in in vitro systems. One technique for the establishment of a long-term cell culture from sponges has recently been elaborated. Here, we present a procedure to cultivate tissue samples from sponges in an open system. The species Geodia cydonium, which produces bioactive compounds, has been selected. Tissue samples of approximately 10 g were attached to the bottoms of cultivation trays. After 2 to 3 days, the tissue samples formed a robust contact with the metal support. Subsequently, sets of trays, called tray batteries, either remained in huge aquaria at the Center for Marine Research or were transferred to the vicinity of a fish and mussel farm. The growth rates of the samples remained unchanged within the first month; however, after 3 and 6 months, they increased to 147% and 189%, respectively. In parallel, extracts were prepared from the tissue samples and tested for cytotoxicity in a mouse lymphoma cell assay system. Extracts from cultured tissue initially had a low inhibitory potency; however, after cultivation for 3 or 6 months, values comparable to those of extracts from sponges taken from the biotope were found. In addition, a molecular marker was applied to document the response (health state) of the tissue and the identity of the material in culture. The CD63 molecule was chosen because the expression of this molecule in mammalian systems changes with the age of the animals. The corresponding complementary DNA was isolated from Geodia cydonium. With this probe, the level of expression in cultured tissue samples decreased immediately after starting cultivation; after a cultivation period of 6 months, however, values were similar to those found in controls. These data show that sponge species that produce bioactive compounds can be cultivated in open systems, in which they retain their potency to produce bioactive compounds as well as their health state.
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origin of metazoan adhesion molecules and adhesion receptors as deduced from cdna analyses in the marine sponge Geodia cydonium a review
Cell and Tissue Research, 1997Co-Authors: Werner E. G. MüllerAbstract:The phylogenetic relationships of the kingdom Animalia (Metazoa) have long been questioned. Whether the lowest eukaryotic multicellular organisms, the metazoan phylum Porifera (sponges), independently evolved multicellularity from a separate protist lineage (polyphyly of animals) or whether they were derived from the same protist group as the other animal phyla (monophyly) remains unclear. Analyses of the genes that are typical for multicellularity, e.g. those coding for adhesion molecules (galectin) and adhesion receptors (receptor tyrosine kinase, integrin receptor, receptors featuring scavenger receptor cysteine-rich domains) or elements involved in signal transduction pathways (G-proteins, Ser/Thr protein kinases), especially from the marine sponge Geodia cydonium, indicate that all animals, including sponges, are of monophyletic origin.
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genome size and chromosomes in marine sponges suberites domuncula Geodia cydonium
Cell Biology International, 1995Co-Authors: Georg Imsiecke, Renate Steffen, Marcio R Custodio, Radovan Borojevic, Mohamed A Moustafa, Werner E. G. MüllerAbstract:The genome size of the marine sponges Suberites domuncula and Geodia cydonium has been determined by flow cytofluorometric analysis using diamidino-phenylindole [DAPI]. Using human lymphocytes as reference the amount of DNA in cells from S. domuncula has been determined to be 3.7 pg and that of G. cydonium 3.3 pg. While no chromosomes could be identified in G. cydonium, the karyotype of the Suberites domuncula is 32 chromsomes in the diploid state. The size of the chromosomes was between 0.25 and 1.0 μm. No pronounced banding pattern was visible.
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immunoglobulin like domain is present in the extracellular part of the receptor tyrosine kinase from the marine sponge Geodia cydonium
Journal of Molecular Recognition, 1994Co-Authors: Heike Schacke, Isabel M. Müller, Baruch Rinkevich, Vera Gamulin, Werner E. G. MüllerAbstract:We have isolated and characterized two cDNAs from the marine sponge Geodia cydonium coding for a new member of a receptor tyrosine kinase of class II. The deduced amino acid sequence shows two characteristic domains: (i) the tyrosine kinase domain; and (ii) and immunoglobulin-like domain. The latter part shows high homology to the vertebrate C2 type immunoglobulin domain. This result demonstrates that immunoglobulin domains are not recent achievements of higher animals but exist also in those animals which have diverged from other organisms about 800 million years ago.
Hans Tore Rapp - One of the best experts on this subject based on the ideXlab platform.
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reproductive biology of Geodia species porifera tetractinellida from boreo arctic north atlantic deep sea sponge grounds
Frontiers in Marine Science, 2020Co-Authors: Hans Tore Rapp, Paco Cárdenas, Vasiliki Koutsouveli, Maria Conejero, Ana RiesgoAbstract:Reproductive biology of Geodia (Porifera, Tetractinellida) from boreo-arctic North-Atlantic deep-sea sponge grounds
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Geodia boreo-arctic distribution
2016Co-Authors: Paco Cárdenas, Hans Tore Rapp, Megan Best, Mikael Thollesson, Anne Birgitte Klitgaard, Ole Secher TendalAbstract:This excel file includes locality records for all six species of boreo-arctic Geodia examined by us in various campaigns/museums collections and from the litterature. This file includes geographical coordinates, museum collection or reference, temperature and salinity when available. When the latitude/longitude information was missing but the locality was given, we reconstructed the geographic coordinates using Google Earth. Temperature ranges for each species were obtained from the campaigns in which bottom temperatures were recorded (e.g. Ingolf Exp., BIOICE, BIOFAR, PA1994, PA2010-009) and from the literature
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List of Geodia specimens examined
2016Co-Authors: Paco Cárdenas, Hans Tore Rapp, Megan Best, Mikael Thollesson, Anne Birgitte Klitgaard, Ole Secher TendalAbstract:List of boreo-arctic Geodia specimens identified for this study and used to build the distribution maps: In cases where we have many samples from approximately the same locality only a minor number of records are listed; the number in parentheses is the number of specimens caught at each station
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taxonomy biogeography and dna barcodes of Geodia species porifera demospongiae tetractinellida in the atlantic boreo arctic region
Zoological Journal of the Linnean Society, 2013Co-Authors: Hans Tore Rapp, Paco Cárdenas, Anne B Klitgaard, Megan Best, Mikael Thollesson, Ole Secher TendalAbstract:Geodia species north of 60 degrees N in the Atlantic appeared in the literature for the first time when Bowerbank described Geodia barretti and G.macandrewii in 1858 from western Norway. Since then ...
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molecular taxonomy and phylogeny of the geodiidae porifera demospongiae astrophorida combining phylogenetic and linnaean classification
Zoologica Scripta, 2010Co-Authors: Paco Cairdenas, Hans Tore Rapp, Christoffer Schander, Ole Secher TendalAbstract:Cardenas, P., Rapp, H. T., Schander, C. & Tendal, O. S. (2009). Molecular taxonomy and phylogeny of the Geodiidae (Porifera, Demospongiae, Astrophorida) – combining phylogenetic and Linnaean classification.—Zoologica Scripta, 39, 89–106. According to the fossil records, the Geodiidae represents one of the oldest families of demosponges (Phylum Porifera). There are approximately 220 described extant species, geographically and bathymetrically widely distributed around the world. Species of this family all share a two-layered cortex with ball-shaped spicules called ‘sterrasters’ in the endocortex. However, molecular studies have questioned the monophyly of the group. Moreover, the evolutionary history and the intrafamily relationships of the Geodiidae are not fully resolved. Using a partial sequence of the cytochrome c oxidase subunit 1 (COI) gene and a partial sequence of the 28S rDNA gene (D1–D2 domains), we present the first molecular phylogeny focusing on this group. The congruent results from the two gene fragments suggest that (i) the Geodiidae is monophyletic, (ii) the Erylinae/Geodinae subdivision sensuSollas, 1888 is valid and that (iii) Isops and Sidonops are junior synonyms of Geodia. The synonymization of Isops and Sidonops implies that the oscule/pore morphology as a diagnostic character should be abandoned. Geodia hentscheli nom. nov. has been given for Geodia mesotriaena (Hentschel, 1929). This study served as the basis for a revised phylogenetic classification of the Geodiidae. Well-supported clades led to the establishment of clade names following the PhyloCode. The Geodinae clade is strongly supported and notably composed of DepressioGeodia, Cydonium and Geodia. A morphological synapomorphy of Geodinae is the presence of euasters in the ectocortex. The Erylinae (Erylus, Penares, Caminus and Pachymatisma) form a strongly supported monophyletic group with three morphological synapomorphies: (i) loss of anatriaenes and protriaenes, (ii) microrhabds (or spherules) in the ectocortex and (iii) short-shafted triaenes. The Erylus monophyly is ambiguous. Erylus species are distributed in three well-supported clades. Finally, spicule homology in the cortex of the Geodiidae is discussed.
Ole Secher Tendal - One of the best experts on this subject based on the ideXlab platform.
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Geodia boreo-arctic distribution
2016Co-Authors: Paco Cárdenas, Hans Tore Rapp, Megan Best, Mikael Thollesson, Anne Birgitte Klitgaard, Ole Secher TendalAbstract:This excel file includes locality records for all six species of boreo-arctic Geodia examined by us in various campaigns/museums collections and from the litterature. This file includes geographical coordinates, museum collection or reference, temperature and salinity when available. When the latitude/longitude information was missing but the locality was given, we reconstructed the geographic coordinates using Google Earth. Temperature ranges for each species were obtained from the campaigns in which bottom temperatures were recorded (e.g. Ingolf Exp., BIOICE, BIOFAR, PA1994, PA2010-009) and from the literature
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List of Geodia specimens examined
2016Co-Authors: Paco Cárdenas, Hans Tore Rapp, Megan Best, Mikael Thollesson, Anne Birgitte Klitgaard, Ole Secher TendalAbstract:List of boreo-arctic Geodia specimens identified for this study and used to build the distribution maps: In cases where we have many samples from approximately the same locality only a minor number of records are listed; the number in parentheses is the number of specimens caught at each station
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taxonomy biogeography and dna barcodes of Geodia species porifera demospongiae tetractinellida in the atlantic boreo arctic region
Zoological Journal of the Linnean Society, 2013Co-Authors: Hans Tore Rapp, Paco Cárdenas, Anne B Klitgaard, Megan Best, Mikael Thollesson, Ole Secher TendalAbstract:Geodia species north of 60 degrees N in the Atlantic appeared in the literature for the first time when Bowerbank described Geodia barretti and G.macandrewii in 1858 from western Norway. Since then ...
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molecular taxonomy and phylogeny of the geodiidae porifera demospongiae astrophorida combining phylogenetic and linnaean classification
Zoologica Scripta, 2010Co-Authors: Paco Cairdenas, Hans Tore Rapp, Christoffer Schander, Ole Secher TendalAbstract:Cardenas, P., Rapp, H. T., Schander, C. & Tendal, O. S. (2009). Molecular taxonomy and phylogeny of the Geodiidae (Porifera, Demospongiae, Astrophorida) – combining phylogenetic and Linnaean classification.—Zoologica Scripta, 39, 89–106. According to the fossil records, the Geodiidae represents one of the oldest families of demosponges (Phylum Porifera). There are approximately 220 described extant species, geographically and bathymetrically widely distributed around the world. Species of this family all share a two-layered cortex with ball-shaped spicules called ‘sterrasters’ in the endocortex. However, molecular studies have questioned the monophyly of the group. Moreover, the evolutionary history and the intrafamily relationships of the Geodiidae are not fully resolved. Using a partial sequence of the cytochrome c oxidase subunit 1 (COI) gene and a partial sequence of the 28S rDNA gene (D1–D2 domains), we present the first molecular phylogeny focusing on this group. The congruent results from the two gene fragments suggest that (i) the Geodiidae is monophyletic, (ii) the Erylinae/Geodinae subdivision sensuSollas, 1888 is valid and that (iii) Isops and Sidonops are junior synonyms of Geodia. The synonymization of Isops and Sidonops implies that the oscule/pore morphology as a diagnostic character should be abandoned. Geodia hentscheli nom. nov. has been given for Geodia mesotriaena (Hentschel, 1929). This study served as the basis for a revised phylogenetic classification of the Geodiidae. Well-supported clades led to the establishment of clade names following the PhyloCode. The Geodinae clade is strongly supported and notably composed of DepressioGeodia, Cydonium and Geodia. A morphological synapomorphy of Geodinae is the presence of euasters in the ectocortex. The Erylinae (Erylus, Penares, Caminus and Pachymatisma) form a strongly supported monophyletic group with three morphological synapomorphies: (i) loss of anatriaenes and protriaenes, (ii) microrhabds (or spherules) in the ectocortex and (iii) short-shafted triaenes. The Erylus monophyly is ambiguous. Erylus species are distributed in three well-supported clades. Finally, spicule homology in the cortex of the Geodiidae is discussed.
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sexual reproduction of Geodia barretti bowerbank 1858 porifera astrophorida in two scandinavian fjords
2007Co-Authors: F Spetland, Friederike Hoffmann, Hans Tore Rapp, Ole Secher TendalAbstract:The gametogenesis in the common cold-water sponge Geodia barretti is described from two Scandinavian fjords through a year cycle: in Korsfjorden, western Norway, and in Kosterfjorden on the Swedish west-coast. The reproductive cycle is annual for both populations, with one or two periods of gamete release per year. Individuals within the same local population reproduce simultaneously within a restricted period of time. Geodia barretti is a dioecious and oviparous sponge, with oocytes (up to 100 µm in diameter) and spermatic cysts (up to 125 µm in diameter) organised in clusters within the mesohyl. The sponge has asynchronous spermatogenesis and synchronous oogenesis. Asexual reproduction has not been observed. The onset of the reproduction coincides with the phytoplankton blooms in both fjords: Gametes are released in early summer, just after the phytoplankton spring bloom is over. In Kosterfjorden, however, an additional release of gametes occurs in October, just after the autumn phytoplankton bloom. In both fjord systems reproduction of G. barretti thus matches the peaks in sedimentation of organic matter that follow after phytoplankton blooms.
Nan Chen - One of the best experts on this subject based on the ideXlab platform.
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Permanent Genetic Resources added to Molecular Ecology Resources Database 1 December 2009-31 January 2010.
Molecular Ecology Resources, 2010Co-Authors: Cynthia M. Anderson, Gallego J. Aparicio, Alain R. Atangana, Jean Beaulieu, Michael W. Bruford, Forest Cain, T. Campos, A. Cariani, M.a. Carvalho, Nan ChenAbstract:This article documents the addition of 220 microsatellite marker loci to the Molecular Ecology Resources Database. Loci were developed for the following species: Allanblackia floribunda, Amblyraja radiata, Bactrocera cucurbitae, Brachycaudus helichrysi, Calopogonium mucunoides, Dissodactylus primitivus, Elodea canadensis, Ephydatia fluviatilis, Galapaganus howdenae howdenae, Hoplostethus atlanticus, Ischnura elegans, Larimichthys polyactis, Opheodrys vernalis, Pelteobagrus fulvidraco, Phragmidium violaceum, Pistacia vera, and Thunnus thynnus. These loci were cross-tested on the following species: Allanblackia gabonensis, Allanblackia stanerana, Neoceratitis cyanescens, Dacus ciliatus, Dacus demmerezi, Bactrocera zonata, Ceratitis capitata, Ceratitis rosa, Ceratits catoirii, Dacus punctatifrons, Ephydatia mülleri, Spongilla lacustris, Geodia cydonium, Axinella sp., Ischnura graellsii, Ischnura ramburii, Ischnura pumilio, Pistacia integerrima and Pistacia terebinthus.