The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Donatella Mares - One of the best experts on this subject based on the ideXlab platform.
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Antidermatophytic activity of pyrazolo[3,4-c]isothiazoles: a preliminary approach on 4-chlorophenyl derivative for evaluation of mutagenic and clastogenic effects on bacteria and human chromosomes in vitro.
Drug and chemical toxicology, 2011Co-Authors: Damiano Rossi, Donatella Mares, Carlo Romagnoli, Elisa Andreotti, Stefano Manfredini, Chiara Beatrice VicentiniAbstract:The antifungal activity of eight pyrazolo[3,4-c]isothiazole derivatives was evaluated on five dermatophytes: three were of an anthropophilic species (i.e., Epidermophyton floccosum, Trichophyton rubrum, and Trichophyton tonsurans) and two were of a Geophilic species (i.e., Microsporum gypseum and Nannizzia cajetani). The new compounds proved to be unlikely effective in inhibiting the growth of the different strains. In general, the fungi parasitic on man were more sensitive than the Geophilic species. This fact can be positive for a possible practical-therapeutic utilization of this class of compounds. To verify their possible use against fungi of medical interest, the most interesting substance at low doses, 6-(4-chlorophenyl)-4-methyl-6H-pyrazolo[3,4-c]isothiazol-3-amine, was chosen to perform in vitro genotoxicity tests using the following: Salmonella/microsome test (SAL), sister chromatid excange test (SCE), cytokinesis-blocked micronucleus test (CBMN), and its improvement (Ara-C/CBMN). The compound s...
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Antidermatophytic activity of pyrazolo[3,4-c]isothiazoles: a preliminary approach on 4-chlorophenyl derivative for evaluation of mutagenic and clastogenic effects on bacteria and human chromosomes in vitro
'Informa UK Limited', 2011Co-Authors: Damiano Rossi, Donatella Mares, Carlo Romagnoli, Elisa Andreotti, Stefano Manfredini, Chiara Beatrice VicentiniAbstract:The antifungal activity of eight pyrazolo[3,4-c]isothiazole derivatives was evaluated on five dermatophytes: three were of an anthropophilic species (i.e., Epidermophyton floccosum, Trichophyton rubrum, and Trichophyton tonsurans) and two were of a Geophilic species (i.e., Microsporum gypseum and Nannizzia cajetani). The new compounds proved to be unlikely effective in inhibiting the growth of the different strains. In general, the fungi parasitic on man were more sensitive than the Geophilic species. This fact can be positive for a possible practical-therapeutic utilization of this class of compounds. To verify their possible use against fungi of medical interest, the most interesting substance at low doses, 6-(4-chlorophenyl)-4-methyl-6H-pyrazolo[3,4-c]isothiazol-3-amine, was chosen to perform in vitro genotoxicity tests using the following: Salmonella/microsome test (SAL), sister chromatid excange test (SCE), cytokinesis-blocked micronucleus test (CBMN), and its improvement (Ara-C/CBMN). The compound showed no mutagenic activity at low doses, whereas at the highest dose (100 µg/mL), it caused a generalized cytotoxic effect. The high growth inhibition exerted on fungi at the lowest dose and the concomitant lack of genotoxicity, at least until the dose of 50 µg/mL, might suggest the compound as a safe candidate as an antidermatophytic substance
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Antidermatophytic activity of pyrazolo[3,4-c]isothiazoles: a preliminary approach on 4-chlorophenyl derivative for evaluation of mutagenic and clastogenic effects on bacteria and human chromosomes in vitro
'Informa UK Limited', 2011Co-Authors: Damiano Rossi, Donatella Mares, Stefano Manfredini, Romagnoli Carlo, Andreotti Elisa, B. C. VicentiniAbstract:The antifungal activity of eight pyrazolo[3,4-c]isothiazole derivatives was evaluated on five 21 dermatophytes: three are anthropophilic species, i.e. Epidermophyton floccosum, Trichophyton 22 rubrum and Trichophyton tonsurans, and two are Geophilic species, Microsporum gypseum and 23 Nannizia cajetani. The new compounds proved to be unlikely effective in inhibiting the growth of 24 the different strains. In general the fungi parasitic on man were more sensitive than the Geophilic25 species. This fact can be positive for a possible practical-therapeutic utilization of these class of 26 compounds. To verify their possible use against fungi of medical interest, the most interesting 27 substance at low doses, 6-(4-chlorophenyl)-4-methyl-6H-pyrazolo[3,4-c]isothiazol-3-amine, was 28 chosen to perform in vitro genotoxicity tests using: Salmonella/microsome test (SAL), Sister 29 Chromatid Excange test (SCE), Cytokinesis-Blocked Micronucleus test (CBMN) and its 30 improvement (Ara-C/CBMN). The compound showed no mutagenic activity at low doses, whereas 31 at the highest dose (100 \u3bcg/mL) it causes a generalized cytotoxic effect. The high growth inhibition32 exerted on fungi at the lowest dose and the concomitant lack of genotoxicity, at least until the dose 33 of 50 \u3bcg/mL, might suggest the compound as a safe candidate as antidermatophytic substance
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chicory extracts from cichorium intybus l as potential antifungals
Mycopathologia, 2005Co-Authors: Donatella Mares, Carlo Romagnoli, Elisa Andreotti, B Tosi, G Chillemi, Ferruccio PoliAbstract:In this work extracts from roots of the common vegetable Cichorium intybus L., highly appreciated for its bitter taste, were studied to investigate their possible biological activity on fungi from a variety of ecological environments: some are parasites on plants (phytopathogens) or of animals and humans (zoophilic and anthropophilic dermatophytes), others live on the soil and only seldom parasitize animals (Geophilic dermatophytes). The extracts were ineffective on Geophilic species and on tested phytopathogens, with the exception of Pythium ultimum, whereas they inhibited the growth of zoophilic and anthropophilic dermatophytes, in particular Trichophyton tonsurans var. sulfureum, whose treatment caused morphological anomalies, here observed by scanning electron microscopy. This behaviour is discussed on the basis of the presence in the chicory extract of the two main sesquiterpene lactones, 8-deoxylactucin and 11 beta,13-dihydrolactucin.
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chicory extracts from cichorium intybus l as potential antifungals
Mycopathologia, 2005Co-Authors: Donatella Mares, Carlo Romagnoli, Elisa Andreotti, B Tosi, G Chillemi, Ferruccio PoliAbstract:In this work extracts from roots of the common vegetable Cichorium intybus L., highly appreciated for its bitter taste, were studied to investigate their possible biological activity on fungi from a variety of ecological environments: some are parasites on plants (phytopathogens) or of animals and humans (zoophilic and anthropophilic dermatophytes), others live on the soil and only seldom parasitize animals (Geophilic dermatophytes). The extracts were ineffective on Geophilic species and on tested phytopathogens, with the exception of Pythium ultimum, whereas they inhibited the growth of zoophilic and anthropophilic dermatophytes, in particular Trichophyton tonsurans var. sulfureum, whose treatment caused morphological anomalies, here observed by scanning electron microscopy. This behaviour is discussed on the basis of the presence in the chicory extract of the two main sesquiterpene lactones, 8-deoxylactucin and 11β,13-dihydrolactucin.
Weida Liu - One of the best experts on this subject based on the ideXlab platform.
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assembly and analysis of the whole genome of arthroderma uncinatum strain t10 compared with microsporum canis and trichophyton rubrum
Mycoses, 2020Co-Authors: Hailin Zheng, Oliver Blechert, Huan Mei, Jia Liu, Ye Tao, Sybren G De Hoog, Weida LiuAbstract:Background Arthroderma uncinatum is a Geophilic dermatophyte that occasionally causes superficial infections in humans leading to skin diseases. Objectives To better understand the ecology and potential pathogenicity of A uncinatum, we analysed its whole genome. We compared A uncinatum with the genome of the zoophilic dermatophyte Microsporum canis and with the anthropophilic species Trichophyton rubrum. The compared species differ significantly in the frequency of human infection. Methods We reported the genome sequence of strain T10 of A uncinatum based on SMRT (single-molecule real-time) technology (PacBio). Results We obtained a near-complete 23.56 Mb genome, with 7153 predicted gene models and ~20% repetitive sequences. We subsequently determined the specific genetic differences between A uncinatum, M canis and T rubrum. The functional enrichment analysis suggests that A uncinatum is particularly enriched in specific virulence genes. This suggests that the ancestral condition in dermatophytes is with high virulence, which has decreased in the course of evolution to enhance coexistence with animal or human hosts.
Pier Giuseppe Meneguz - One of the best experts on this subject based on the ideXlab platform.
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Dermatophytosis caused by Microsporum canis in Eastern cottontail (Sylvilagus floridanus)
European Journal of Wildlife Research, 2007Co-Authors: Paolo Tizzani, M. G. Gallo, A. Peano, A. Molinar Min, C. Martínez-carrasco Pleite, Pier Giuseppe MeneguzAbstract:Eastern cottontail ( Sylvilagus floridanus , fam. Leporidae) has previously been shown to be a potential healthy carrier of dermatophyte fungi both Geophilic ( Microsporum gypseum , M. cookei , Trichophyton ajelloi , T. terrestre ) and zoophilic ( M. canis , T. mentagrophytes ). In this communication, the first case, to the best of our knowledge, of a symptomatic dermatophyte infection in S. floridanus is described.
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Eastern Cottontail ( Sylvilagus Floridanus) as Carrier of Dermatophyte Fungi
Mycopathologia, 2005Co-Authors: M. G. Gallo, Paolo Tizzani, A. Peano, Luisa Rambozzi, Pier Giuseppe MeneguzAbstract:Eastern cottontail (Sylvilagus floridanus, fam. Leporidae), introduced into Piedmont (Italy) in the 1960s, was studied as carrier of dermatophyte fungi. Of 216 hair samples collected from animals culled between September 1999 and July 2000 in the Province of Alessandria (Piedmont, Italy) during a pest control project, 57 (26.4%) yielded dermatophyte colonies. As two different species of dermatophytes grew from two samples, a total of 59 fungal isolates (26.5%) were obtained. Six dermatophyte species both Geophilic (M. gypseum, M. cookei, Trichophyton ajelloi, T. terrestre) and zoophilic (M. canis, T. mentagrophytes) were identified. No sex-related differences were found but season-related differences were observed. The highest prevalence of dermatophyte-positive samples was recorded in May–September, due to the Geophilic fungi whose prevalence decreased during colder and increased during warmer months ( p < 0.001). The presence of zoophilic dermatophytes, T. mentagrophytes, commonly associated with rodents, small mammals and lagomorphs and M. canis, usually correlated with domestic environment, did not change the whole year round. As Eastern cottontail has been showed to be a carrier of dermatophytes transmissible to man (M. canis, T. mentagrophytes and M. gypseum), it may represent a source of infection for gamekeepers, hunters and veterinarians.
Elisa Andreotti - One of the best experts on this subject based on the ideXlab platform.
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Antidermatophytic activity of pyrazolo[3,4-c]isothiazoles: a preliminary approach on 4-chlorophenyl derivative for evaluation of mutagenic and clastogenic effects on bacteria and human chromosomes in vitro.
Drug and chemical toxicology, 2011Co-Authors: Damiano Rossi, Donatella Mares, Carlo Romagnoli, Elisa Andreotti, Stefano Manfredini, Chiara Beatrice VicentiniAbstract:The antifungal activity of eight pyrazolo[3,4-c]isothiazole derivatives was evaluated on five dermatophytes: three were of an anthropophilic species (i.e., Epidermophyton floccosum, Trichophyton rubrum, and Trichophyton tonsurans) and two were of a Geophilic species (i.e., Microsporum gypseum and Nannizzia cajetani). The new compounds proved to be unlikely effective in inhibiting the growth of the different strains. In general, the fungi parasitic on man were more sensitive than the Geophilic species. This fact can be positive for a possible practical-therapeutic utilization of this class of compounds. To verify their possible use against fungi of medical interest, the most interesting substance at low doses, 6-(4-chlorophenyl)-4-methyl-6H-pyrazolo[3,4-c]isothiazol-3-amine, was chosen to perform in vitro genotoxicity tests using the following: Salmonella/microsome test (SAL), sister chromatid excange test (SCE), cytokinesis-blocked micronucleus test (CBMN), and its improvement (Ara-C/CBMN). The compound s...
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Antidermatophytic activity of pyrazolo[3,4-c]isothiazoles: a preliminary approach on 4-chlorophenyl derivative for evaluation of mutagenic and clastogenic effects on bacteria and human chromosomes in vitro
'Informa UK Limited', 2011Co-Authors: Damiano Rossi, Donatella Mares, Carlo Romagnoli, Elisa Andreotti, Stefano Manfredini, Chiara Beatrice VicentiniAbstract:The antifungal activity of eight pyrazolo[3,4-c]isothiazole derivatives was evaluated on five dermatophytes: three were of an anthropophilic species (i.e., Epidermophyton floccosum, Trichophyton rubrum, and Trichophyton tonsurans) and two were of a Geophilic species (i.e., Microsporum gypseum and Nannizzia cajetani). The new compounds proved to be unlikely effective in inhibiting the growth of the different strains. In general, the fungi parasitic on man were more sensitive than the Geophilic species. This fact can be positive for a possible practical-therapeutic utilization of this class of compounds. To verify their possible use against fungi of medical interest, the most interesting substance at low doses, 6-(4-chlorophenyl)-4-methyl-6H-pyrazolo[3,4-c]isothiazol-3-amine, was chosen to perform in vitro genotoxicity tests using the following: Salmonella/microsome test (SAL), sister chromatid excange test (SCE), cytokinesis-blocked micronucleus test (CBMN), and its improvement (Ara-C/CBMN). The compound showed no mutagenic activity at low doses, whereas at the highest dose (100 µg/mL), it caused a generalized cytotoxic effect. The high growth inhibition exerted on fungi at the lowest dose and the concomitant lack of genotoxicity, at least until the dose of 50 µg/mL, might suggest the compound as a safe candidate as an antidermatophytic substance
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chicory extracts from cichorium intybus l as potential antifungals
Mycopathologia, 2005Co-Authors: Donatella Mares, Carlo Romagnoli, Elisa Andreotti, B Tosi, G Chillemi, Ferruccio PoliAbstract:In this work extracts from roots of the common vegetable Cichorium intybus L., highly appreciated for its bitter taste, were studied to investigate their possible biological activity on fungi from a variety of ecological environments: some are parasites on plants (phytopathogens) or of animals and humans (zoophilic and anthropophilic dermatophytes), others live on the soil and only seldom parasitize animals (Geophilic dermatophytes). The extracts were ineffective on Geophilic species and on tested phytopathogens, with the exception of Pythium ultimum, whereas they inhibited the growth of zoophilic and anthropophilic dermatophytes, in particular Trichophyton tonsurans var. sulfureum, whose treatment caused morphological anomalies, here observed by scanning electron microscopy. This behaviour is discussed on the basis of the presence in the chicory extract of the two main sesquiterpene lactones, 8-deoxylactucin and 11 beta,13-dihydrolactucin.
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chicory extracts from cichorium intybus l as potential antifungals
Mycopathologia, 2005Co-Authors: Donatella Mares, Carlo Romagnoli, Elisa Andreotti, B Tosi, G Chillemi, Ferruccio PoliAbstract:In this work extracts from roots of the common vegetable Cichorium intybus L., highly appreciated for its bitter taste, were studied to investigate their possible biological activity on fungi from a variety of ecological environments: some are parasites on plants (phytopathogens) or of animals and humans (zoophilic and anthropophilic dermatophytes), others live on the soil and only seldom parasitize animals (Geophilic dermatophytes). The extracts were ineffective on Geophilic species and on tested phytopathogens, with the exception of Pythium ultimum, whereas they inhibited the growth of zoophilic and anthropophilic dermatophytes, in particular Trichophyton tonsurans var. sulfureum, whose treatment caused morphological anomalies, here observed by scanning electron microscopy. This behaviour is discussed on the basis of the presence in the chicory extract of the two main sesquiterpene lactones, 8-deoxylactucin and 11β,13-dihydrolactucin.
Carlo Romagnoli - One of the best experts on this subject based on the ideXlab platform.
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Antidermatophytic activity of pyrazolo[3,4-c]isothiazoles: a preliminary approach on 4-chlorophenyl derivative for evaluation of mutagenic and clastogenic effects on bacteria and human chromosomes in vitro.
Drug and chemical toxicology, 2011Co-Authors: Damiano Rossi, Donatella Mares, Carlo Romagnoli, Elisa Andreotti, Stefano Manfredini, Chiara Beatrice VicentiniAbstract:The antifungal activity of eight pyrazolo[3,4-c]isothiazole derivatives was evaluated on five dermatophytes: three were of an anthropophilic species (i.e., Epidermophyton floccosum, Trichophyton rubrum, and Trichophyton tonsurans) and two were of a Geophilic species (i.e., Microsporum gypseum and Nannizzia cajetani). The new compounds proved to be unlikely effective in inhibiting the growth of the different strains. In general, the fungi parasitic on man were more sensitive than the Geophilic species. This fact can be positive for a possible practical-therapeutic utilization of this class of compounds. To verify their possible use against fungi of medical interest, the most interesting substance at low doses, 6-(4-chlorophenyl)-4-methyl-6H-pyrazolo[3,4-c]isothiazol-3-amine, was chosen to perform in vitro genotoxicity tests using the following: Salmonella/microsome test (SAL), sister chromatid excange test (SCE), cytokinesis-blocked micronucleus test (CBMN), and its improvement (Ara-C/CBMN). The compound s...
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Antidermatophytic activity of pyrazolo[3,4-c]isothiazoles: a preliminary approach on 4-chlorophenyl derivative for evaluation of mutagenic and clastogenic effects on bacteria and human chromosomes in vitro
'Informa UK Limited', 2011Co-Authors: Damiano Rossi, Donatella Mares, Carlo Romagnoli, Elisa Andreotti, Stefano Manfredini, Chiara Beatrice VicentiniAbstract:The antifungal activity of eight pyrazolo[3,4-c]isothiazole derivatives was evaluated on five dermatophytes: three were of an anthropophilic species (i.e., Epidermophyton floccosum, Trichophyton rubrum, and Trichophyton tonsurans) and two were of a Geophilic species (i.e., Microsporum gypseum and Nannizzia cajetani). The new compounds proved to be unlikely effective in inhibiting the growth of the different strains. In general, the fungi parasitic on man were more sensitive than the Geophilic species. This fact can be positive for a possible practical-therapeutic utilization of this class of compounds. To verify their possible use against fungi of medical interest, the most interesting substance at low doses, 6-(4-chlorophenyl)-4-methyl-6H-pyrazolo[3,4-c]isothiazol-3-amine, was chosen to perform in vitro genotoxicity tests using the following: Salmonella/microsome test (SAL), sister chromatid excange test (SCE), cytokinesis-blocked micronucleus test (CBMN), and its improvement (Ara-C/CBMN). The compound showed no mutagenic activity at low doses, whereas at the highest dose (100 µg/mL), it caused a generalized cytotoxic effect. The high growth inhibition exerted on fungi at the lowest dose and the concomitant lack of genotoxicity, at least until the dose of 50 µg/mL, might suggest the compound as a safe candidate as an antidermatophytic substance
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chicory extracts from cichorium intybus l as potential antifungals
Mycopathologia, 2005Co-Authors: Donatella Mares, Carlo Romagnoli, Elisa Andreotti, B Tosi, G Chillemi, Ferruccio PoliAbstract:In this work extracts from roots of the common vegetable Cichorium intybus L., highly appreciated for its bitter taste, were studied to investigate their possible biological activity on fungi from a variety of ecological environments: some are parasites on plants (phytopathogens) or of animals and humans (zoophilic and anthropophilic dermatophytes), others live on the soil and only seldom parasitize animals (Geophilic dermatophytes). The extracts were ineffective on Geophilic species and on tested phytopathogens, with the exception of Pythium ultimum, whereas they inhibited the growth of zoophilic and anthropophilic dermatophytes, in particular Trichophyton tonsurans var. sulfureum, whose treatment caused morphological anomalies, here observed by scanning electron microscopy. This behaviour is discussed on the basis of the presence in the chicory extract of the two main sesquiterpene lactones, 8-deoxylactucin and 11 beta,13-dihydrolactucin.
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chicory extracts from cichorium intybus l as potential antifungals
Mycopathologia, 2005Co-Authors: Donatella Mares, Carlo Romagnoli, Elisa Andreotti, B Tosi, G Chillemi, Ferruccio PoliAbstract:In this work extracts from roots of the common vegetable Cichorium intybus L., highly appreciated for its bitter taste, were studied to investigate their possible biological activity on fungi from a variety of ecological environments: some are parasites on plants (phytopathogens) or of animals and humans (zoophilic and anthropophilic dermatophytes), others live on the soil and only seldom parasitize animals (Geophilic dermatophytes). The extracts were ineffective on Geophilic species and on tested phytopathogens, with the exception of Pythium ultimum, whereas they inhibited the growth of zoophilic and anthropophilic dermatophytes, in particular Trichophyton tonsurans var. sulfureum, whose treatment caused morphological anomalies, here observed by scanning electron microscopy. This behaviour is discussed on the basis of the presence in the chicory extract of the two main sesquiterpene lactones, 8-deoxylactucin and 11β,13-dihydrolactucin.