The Experts below are selected from a list of 243 Experts worldwide ranked by ideXlab platform
Pietro Alifano - One of the best experts on this subject based on the ideXlab platform.
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Effects induced by XeCl laser radiation and Germicidal Lamp radiation on E. coli strains survival and mutability
Biophotonics and New Therapy Frontiers, 2006Co-Authors: Fabio Belloni, Adelfia Talà, Antonella Lorusso, Caterina Monaco, Pietro Alifano, Vincenzo Nassisi, M. TrediciAbstract:In this work the mutagenic effect on Escherichia coli strains induced by UV radiation emitted by a XeCl laser (λ = 308 nm) has been analysed as a function of the exposure dose and compared with the effect induced by 254 nm radiation emitted by a conventional Germicidal Lamp. E. coli strains, wild-type (recA+ ) and mutant (recA1 , defective in DNA damage repair systems), plated on LB agar, supplemented with rifampicin when requested, were irradiated by means of a Germicidal Lamp in the dose range 0 - 9 mJ/cm2. Similar strains were exposed to 308 nm pulsed laser radiation (τ = 20 ns FWHM; max. pulse energy: 100 mJ) in the dose range 0-1.0 x 104 mJ/cm2. The discrepancy between the results obtained with the Lamp and the laser on the mutation frequency, suggested that the biological response to the two radiation sources involves distinct mechanisms. This hypothesis was supported by the evidence that exposure to near-UV 308 nm induced mutagenesis in the recA-defective strain at an extent considerably higher than in the recA-proficient strain.© (2006) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.
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Effects of XeCl UV308 nm laser radiation on survival and mutability of recA-proficient and recA-defective Escherichia coli strains.
Radiation research, 2006Co-Authors: Adelfia Talà, Antonella Lorusso, Caterina Monaco, Fabio Belloni, Vincenzo Nassisi, Pietro AlifanoAbstract:recA1, recA13 and recA56 are considered null alleles of the Escherichia coli recA gene because they were shown to have essentially no activity in vivo. In this study, we used strains harboring the recA null alleles and their recA-proficient congenic counterpart to assess the lethal and the mutagenic effects elicited by near-UV(308 nm) coherent radiation generated by a XeCl excimer laser. We compared these effects with those produced by a conventional far-UV(254 nm) Germicidal Lamp. Compared to the Germicidal Lamp, the excimer laser was able to better discriminate the different recA-defective strains on the basis of their UV-radiation sensitivity, which was progressively higher in the strains with the alleles in the order recA1, recA56 and recA13. This finding was consistent with previous data on residual biochemical activities of the respective mutated RecA proteins in vitro. The discrepancy between the results obtained with the Lamp and laser irradiation suggested that the biological response to the two radiations involves distinct mechanisms. This hypothesis was supported by the evidence that exposure to near-UV(308 nm) radiation induced mutagenesis in recA-defective strains at an extent considerably greater than in recA-proficient strains. In contrast, far-UV(254 nm)-radiation-induced mutagenesis was reported to be largely dependent on a functional recA allele.
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Effects of XeCl UV308 nm Laser Radiation on Survival and Mutability of recA-Proficient and recA-Defective Escherichia coli Strains
Radiation research, 2006Co-Authors: Adelfia Talà, Antonella Lorusso, Caterina Monaco, Fabio Belloni, Vincenzo Nassisi, Pietro AlifanoAbstract:Abstract Tala, A., Belloni, F., Monaco, C., Lorusso, A., Nassisi, V. and Alifano, P. Effects of XeCl UV308 nm Laser Radiation on Survival and Mutability of recA-Proficient and recA-Defective Escherichia coli Strains. Radiat. Res. 165, 532–537 (2006). recA1, recA13 and recA56 are considered null alleles of the Escherichia coli recA gene because they were shown to have essentially no activity in vivo. In this study, we used strains harboring the recA null alleles and their recA-proficient congenic counterpart to assess the lethal and the mutagenic effects elicited by near-UV308 nm coherent radiation generated by a XeCl excimer laser. We compared these effects with those produced by a conventional far-UV254 nm Germicidal Lamp. Compared to the Germicidal Lamp, the excimer laser was able to better discriminate the different recA-defective strains on the basis of their UV-radiation sensitivity, which was progressively higher in the strains with the alleles in the order recA1, recA56 and recA13. This finding was...
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Mutagenesis induced by XeCl laser radiation
CLEO Europe. 2005 Conference on Lasers and Electro-Optics Europe 2005., 2005Co-Authors: F. Belloni, Adelfia Talà, Pietro Alifano, Vincenzo Nassisi, D. Doria, A. Lorusso, C. Monaco, M. TrediciAbstract:In this work the mutagenic effect on Escherichia coli strains of UV radiation emitted by a XeCI laser (lambda=308 nm, tau=20 ns, 100 mJ pulse energy) has been analyzed as a function of the exposure dose and compared with the effect induced by 254 nm radiation emitted by a conventional Germicidal Lamp. Mutations can involve any genome site and therefore can give rise to various phenotypes, which then can be suitably selected. As a consequence, the impact of the induced mutagenesis is outstanding, both in scientific and industrial fields. In particular suitable doses of UV radiation can induce mutations, while higher doses can cause cell death, due to the induction of manifold damages to DNA
Adelfia Talà - One of the best experts on this subject based on the ideXlab platform.
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Effects induced by XeCl laser radiation and Germicidal Lamp radiation on E. coli strains survival and mutability
Biophotonics and New Therapy Frontiers, 2006Co-Authors: Fabio Belloni, Adelfia Talà, Antonella Lorusso, Caterina Monaco, Pietro Alifano, Vincenzo Nassisi, M. TrediciAbstract:In this work the mutagenic effect on Escherichia coli strains induced by UV radiation emitted by a XeCl laser (λ = 308 nm) has been analysed as a function of the exposure dose and compared with the effect induced by 254 nm radiation emitted by a conventional Germicidal Lamp. E. coli strains, wild-type (recA+ ) and mutant (recA1 , defective in DNA damage repair systems), plated on LB agar, supplemented with rifampicin when requested, were irradiated by means of a Germicidal Lamp in the dose range 0 - 9 mJ/cm2. Similar strains were exposed to 308 nm pulsed laser radiation (τ = 20 ns FWHM; max. pulse energy: 100 mJ) in the dose range 0-1.0 x 104 mJ/cm2. The discrepancy between the results obtained with the Lamp and the laser on the mutation frequency, suggested that the biological response to the two radiation sources involves distinct mechanisms. This hypothesis was supported by the evidence that exposure to near-UV 308 nm induced mutagenesis in the recA-defective strain at an extent considerably higher than in the recA-proficient strain.© (2006) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.
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Effects of XeCl UV308 nm laser radiation on survival and mutability of recA-proficient and recA-defective Escherichia coli strains.
Radiation research, 2006Co-Authors: Adelfia Talà, Antonella Lorusso, Caterina Monaco, Fabio Belloni, Vincenzo Nassisi, Pietro AlifanoAbstract:recA1, recA13 and recA56 are considered null alleles of the Escherichia coli recA gene because they were shown to have essentially no activity in vivo. In this study, we used strains harboring the recA null alleles and their recA-proficient congenic counterpart to assess the lethal and the mutagenic effects elicited by near-UV(308 nm) coherent radiation generated by a XeCl excimer laser. We compared these effects with those produced by a conventional far-UV(254 nm) Germicidal Lamp. Compared to the Germicidal Lamp, the excimer laser was able to better discriminate the different recA-defective strains on the basis of their UV-radiation sensitivity, which was progressively higher in the strains with the alleles in the order recA1, recA56 and recA13. This finding was consistent with previous data on residual biochemical activities of the respective mutated RecA proteins in vitro. The discrepancy between the results obtained with the Lamp and laser irradiation suggested that the biological response to the two radiations involves distinct mechanisms. This hypothesis was supported by the evidence that exposure to near-UV(308 nm) radiation induced mutagenesis in recA-defective strains at an extent considerably greater than in recA-proficient strains. In contrast, far-UV(254 nm)-radiation-induced mutagenesis was reported to be largely dependent on a functional recA allele.
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Effects of XeCl UV308 nm Laser Radiation on Survival and Mutability of recA-Proficient and recA-Defective Escherichia coli Strains
Radiation research, 2006Co-Authors: Adelfia Talà, Antonella Lorusso, Caterina Monaco, Fabio Belloni, Vincenzo Nassisi, Pietro AlifanoAbstract:Abstract Tala, A., Belloni, F., Monaco, C., Lorusso, A., Nassisi, V. and Alifano, P. Effects of XeCl UV308 nm Laser Radiation on Survival and Mutability of recA-Proficient and recA-Defective Escherichia coli Strains. Radiat. Res. 165, 532–537 (2006). recA1, recA13 and recA56 are considered null alleles of the Escherichia coli recA gene because they were shown to have essentially no activity in vivo. In this study, we used strains harboring the recA null alleles and their recA-proficient congenic counterpart to assess the lethal and the mutagenic effects elicited by near-UV308 nm coherent radiation generated by a XeCl excimer laser. We compared these effects with those produced by a conventional far-UV254 nm Germicidal Lamp. Compared to the Germicidal Lamp, the excimer laser was able to better discriminate the different recA-defective strains on the basis of their UV-radiation sensitivity, which was progressively higher in the strains with the alleles in the order recA1, recA56 and recA13. This finding was...
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Mutagenesis induced by XeCl laser radiation
CLEO Europe. 2005 Conference on Lasers and Electro-Optics Europe 2005., 2005Co-Authors: F. Belloni, Adelfia Talà, Pietro Alifano, Vincenzo Nassisi, D. Doria, A. Lorusso, C. Monaco, M. TrediciAbstract:In this work the mutagenic effect on Escherichia coli strains of UV radiation emitted by a XeCI laser (lambda=308 nm, tau=20 ns, 100 mJ pulse energy) has been analyzed as a function of the exposure dose and compared with the effect induced by 254 nm radiation emitted by a conventional Germicidal Lamp. Mutations can involve any genome site and therefore can give rise to various phenotypes, which then can be suitably selected. As a consequence, the impact of the induced mutagenesis is outstanding, both in scientific and industrial fields. In particular suitable doses of UV radiation can induce mutations, while higher doses can cause cell death, due to the induction of manifold damages to DNA
Vincenzo Nassisi - One of the best experts on this subject based on the ideXlab platform.
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Effects induced by XeCl laser radiation and Germicidal Lamp radiation on E. coli strains survival and mutability
Biophotonics and New Therapy Frontiers, 2006Co-Authors: Fabio Belloni, Adelfia Talà, Antonella Lorusso, Caterina Monaco, Pietro Alifano, Vincenzo Nassisi, M. TrediciAbstract:In this work the mutagenic effect on Escherichia coli strains induced by UV radiation emitted by a XeCl laser (λ = 308 nm) has been analysed as a function of the exposure dose and compared with the effect induced by 254 nm radiation emitted by a conventional Germicidal Lamp. E. coli strains, wild-type (recA+ ) and mutant (recA1 , defective in DNA damage repair systems), plated on LB agar, supplemented with rifampicin when requested, were irradiated by means of a Germicidal Lamp in the dose range 0 - 9 mJ/cm2. Similar strains were exposed to 308 nm pulsed laser radiation (τ = 20 ns FWHM; max. pulse energy: 100 mJ) in the dose range 0-1.0 x 104 mJ/cm2. The discrepancy between the results obtained with the Lamp and the laser on the mutation frequency, suggested that the biological response to the two radiation sources involves distinct mechanisms. This hypothesis was supported by the evidence that exposure to near-UV 308 nm induced mutagenesis in the recA-defective strain at an extent considerably higher than in the recA-proficient strain.© (2006) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.
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Effects of XeCl UV308 nm laser radiation on survival and mutability of recA-proficient and recA-defective Escherichia coli strains.
Radiation research, 2006Co-Authors: Adelfia Talà, Antonella Lorusso, Caterina Monaco, Fabio Belloni, Vincenzo Nassisi, Pietro AlifanoAbstract:recA1, recA13 and recA56 are considered null alleles of the Escherichia coli recA gene because they were shown to have essentially no activity in vivo. In this study, we used strains harboring the recA null alleles and their recA-proficient congenic counterpart to assess the lethal and the mutagenic effects elicited by near-UV(308 nm) coherent radiation generated by a XeCl excimer laser. We compared these effects with those produced by a conventional far-UV(254 nm) Germicidal Lamp. Compared to the Germicidal Lamp, the excimer laser was able to better discriminate the different recA-defective strains on the basis of their UV-radiation sensitivity, which was progressively higher in the strains with the alleles in the order recA1, recA56 and recA13. This finding was consistent with previous data on residual biochemical activities of the respective mutated RecA proteins in vitro. The discrepancy between the results obtained with the Lamp and laser irradiation suggested that the biological response to the two radiations involves distinct mechanisms. This hypothesis was supported by the evidence that exposure to near-UV(308 nm) radiation induced mutagenesis in recA-defective strains at an extent considerably greater than in recA-proficient strains. In contrast, far-UV(254 nm)-radiation-induced mutagenesis was reported to be largely dependent on a functional recA allele.
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Effects of XeCl UV308 nm Laser Radiation on Survival and Mutability of recA-Proficient and recA-Defective Escherichia coli Strains
Radiation research, 2006Co-Authors: Adelfia Talà, Antonella Lorusso, Caterina Monaco, Fabio Belloni, Vincenzo Nassisi, Pietro AlifanoAbstract:Abstract Tala, A., Belloni, F., Monaco, C., Lorusso, A., Nassisi, V. and Alifano, P. Effects of XeCl UV308 nm Laser Radiation on Survival and Mutability of recA-Proficient and recA-Defective Escherichia coli Strains. Radiat. Res. 165, 532–537 (2006). recA1, recA13 and recA56 are considered null alleles of the Escherichia coli recA gene because they were shown to have essentially no activity in vivo. In this study, we used strains harboring the recA null alleles and their recA-proficient congenic counterpart to assess the lethal and the mutagenic effects elicited by near-UV308 nm coherent radiation generated by a XeCl excimer laser. We compared these effects with those produced by a conventional far-UV254 nm Germicidal Lamp. Compared to the Germicidal Lamp, the excimer laser was able to better discriminate the different recA-defective strains on the basis of their UV-radiation sensitivity, which was progressively higher in the strains with the alleles in the order recA1, recA56 and recA13. This finding was...
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Mutagenesis induced by XeCl laser radiation
CLEO Europe. 2005 Conference on Lasers and Electro-Optics Europe 2005., 2005Co-Authors: F. Belloni, Adelfia Talà, Pietro Alifano, Vincenzo Nassisi, D. Doria, A. Lorusso, C. Monaco, M. TrediciAbstract:In this work the mutagenic effect on Escherichia coli strains of UV radiation emitted by a XeCI laser (lambda=308 nm, tau=20 ns, 100 mJ pulse energy) has been analyzed as a function of the exposure dose and compared with the effect induced by 254 nm radiation emitted by a conventional Germicidal Lamp. Mutations can involve any genome site and therefore can give rise to various phenotypes, which then can be suitably selected. As a consequence, the impact of the induced mutagenesis is outstanding, both in scientific and industrial fields. In particular suitable doses of UV radiation can induce mutations, while higher doses can cause cell death, due to the induction of manifold damages to DNA
Fabio Belloni - One of the best experts on this subject based on the ideXlab platform.
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Effects induced by XeCl laser radiation and Germicidal Lamp radiation on E. coli strains survival and mutability
Biophotonics and New Therapy Frontiers, 2006Co-Authors: Fabio Belloni, Adelfia Talà, Antonella Lorusso, Caterina Monaco, Pietro Alifano, Vincenzo Nassisi, M. TrediciAbstract:In this work the mutagenic effect on Escherichia coli strains induced by UV radiation emitted by a XeCl laser (λ = 308 nm) has been analysed as a function of the exposure dose and compared with the effect induced by 254 nm radiation emitted by a conventional Germicidal Lamp. E. coli strains, wild-type (recA+ ) and mutant (recA1 , defective in DNA damage repair systems), plated on LB agar, supplemented with rifampicin when requested, were irradiated by means of a Germicidal Lamp in the dose range 0 - 9 mJ/cm2. Similar strains were exposed to 308 nm pulsed laser radiation (τ = 20 ns FWHM; max. pulse energy: 100 mJ) in the dose range 0-1.0 x 104 mJ/cm2. The discrepancy between the results obtained with the Lamp and the laser on the mutation frequency, suggested that the biological response to the two radiation sources involves distinct mechanisms. This hypothesis was supported by the evidence that exposure to near-UV 308 nm induced mutagenesis in the recA-defective strain at an extent considerably higher than in the recA-proficient strain.© (2006) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.
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Effects of XeCl UV308 nm laser radiation on survival and mutability of recA-proficient and recA-defective Escherichia coli strains.
Radiation research, 2006Co-Authors: Adelfia Talà, Antonella Lorusso, Caterina Monaco, Fabio Belloni, Vincenzo Nassisi, Pietro AlifanoAbstract:recA1, recA13 and recA56 are considered null alleles of the Escherichia coli recA gene because they were shown to have essentially no activity in vivo. In this study, we used strains harboring the recA null alleles and their recA-proficient congenic counterpart to assess the lethal and the mutagenic effects elicited by near-UV(308 nm) coherent radiation generated by a XeCl excimer laser. We compared these effects with those produced by a conventional far-UV(254 nm) Germicidal Lamp. Compared to the Germicidal Lamp, the excimer laser was able to better discriminate the different recA-defective strains on the basis of their UV-radiation sensitivity, which was progressively higher in the strains with the alleles in the order recA1, recA56 and recA13. This finding was consistent with previous data on residual biochemical activities of the respective mutated RecA proteins in vitro. The discrepancy between the results obtained with the Lamp and laser irradiation suggested that the biological response to the two radiations involves distinct mechanisms. This hypothesis was supported by the evidence that exposure to near-UV(308 nm) radiation induced mutagenesis in recA-defective strains at an extent considerably greater than in recA-proficient strains. In contrast, far-UV(254 nm)-radiation-induced mutagenesis was reported to be largely dependent on a functional recA allele.
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Effects of XeCl UV308 nm Laser Radiation on Survival and Mutability of recA-Proficient and recA-Defective Escherichia coli Strains
Radiation research, 2006Co-Authors: Adelfia Talà, Antonella Lorusso, Caterina Monaco, Fabio Belloni, Vincenzo Nassisi, Pietro AlifanoAbstract:Abstract Tala, A., Belloni, F., Monaco, C., Lorusso, A., Nassisi, V. and Alifano, P. Effects of XeCl UV308 nm Laser Radiation on Survival and Mutability of recA-Proficient and recA-Defective Escherichia coli Strains. Radiat. Res. 165, 532–537 (2006). recA1, recA13 and recA56 are considered null alleles of the Escherichia coli recA gene because they were shown to have essentially no activity in vivo. In this study, we used strains harboring the recA null alleles and their recA-proficient congenic counterpart to assess the lethal and the mutagenic effects elicited by near-UV308 nm coherent radiation generated by a XeCl excimer laser. We compared these effects with those produced by a conventional far-UV254 nm Germicidal Lamp. Compared to the Germicidal Lamp, the excimer laser was able to better discriminate the different recA-defective strains on the basis of their UV-radiation sensitivity, which was progressively higher in the strains with the alleles in the order recA1, recA56 and recA13. This finding was...
Caterina Monaco - One of the best experts on this subject based on the ideXlab platform.
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Effects induced by XeCl laser radiation and Germicidal Lamp radiation on E. coli strains survival and mutability
Biophotonics and New Therapy Frontiers, 2006Co-Authors: Fabio Belloni, Adelfia Talà, Antonella Lorusso, Caterina Monaco, Pietro Alifano, Vincenzo Nassisi, M. TrediciAbstract:In this work the mutagenic effect on Escherichia coli strains induced by UV radiation emitted by a XeCl laser (λ = 308 nm) has been analysed as a function of the exposure dose and compared with the effect induced by 254 nm radiation emitted by a conventional Germicidal Lamp. E. coli strains, wild-type (recA+ ) and mutant (recA1 , defective in DNA damage repair systems), plated on LB agar, supplemented with rifampicin when requested, were irradiated by means of a Germicidal Lamp in the dose range 0 - 9 mJ/cm2. Similar strains were exposed to 308 nm pulsed laser radiation (τ = 20 ns FWHM; max. pulse energy: 100 mJ) in the dose range 0-1.0 x 104 mJ/cm2. The discrepancy between the results obtained with the Lamp and the laser on the mutation frequency, suggested that the biological response to the two radiation sources involves distinct mechanisms. This hypothesis was supported by the evidence that exposure to near-UV 308 nm induced mutagenesis in the recA-defective strain at an extent considerably higher than in the recA-proficient strain.© (2006) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.
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Effects of XeCl UV308 nm laser radiation on survival and mutability of recA-proficient and recA-defective Escherichia coli strains.
Radiation research, 2006Co-Authors: Adelfia Talà, Antonella Lorusso, Caterina Monaco, Fabio Belloni, Vincenzo Nassisi, Pietro AlifanoAbstract:recA1, recA13 and recA56 are considered null alleles of the Escherichia coli recA gene because they were shown to have essentially no activity in vivo. In this study, we used strains harboring the recA null alleles and their recA-proficient congenic counterpart to assess the lethal and the mutagenic effects elicited by near-UV(308 nm) coherent radiation generated by a XeCl excimer laser. We compared these effects with those produced by a conventional far-UV(254 nm) Germicidal Lamp. Compared to the Germicidal Lamp, the excimer laser was able to better discriminate the different recA-defective strains on the basis of their UV-radiation sensitivity, which was progressively higher in the strains with the alleles in the order recA1, recA56 and recA13. This finding was consistent with previous data on residual biochemical activities of the respective mutated RecA proteins in vitro. The discrepancy between the results obtained with the Lamp and laser irradiation suggested that the biological response to the two radiations involves distinct mechanisms. This hypothesis was supported by the evidence that exposure to near-UV(308 nm) radiation induced mutagenesis in recA-defective strains at an extent considerably greater than in recA-proficient strains. In contrast, far-UV(254 nm)-radiation-induced mutagenesis was reported to be largely dependent on a functional recA allele.
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Effects of XeCl UV308 nm Laser Radiation on Survival and Mutability of recA-Proficient and recA-Defective Escherichia coli Strains
Radiation research, 2006Co-Authors: Adelfia Talà, Antonella Lorusso, Caterina Monaco, Fabio Belloni, Vincenzo Nassisi, Pietro AlifanoAbstract:Abstract Tala, A., Belloni, F., Monaco, C., Lorusso, A., Nassisi, V. and Alifano, P. Effects of XeCl UV308 nm Laser Radiation on Survival and Mutability of recA-Proficient and recA-Defective Escherichia coli Strains. Radiat. Res. 165, 532–537 (2006). recA1, recA13 and recA56 are considered null alleles of the Escherichia coli recA gene because they were shown to have essentially no activity in vivo. In this study, we used strains harboring the recA null alleles and their recA-proficient congenic counterpart to assess the lethal and the mutagenic effects elicited by near-UV308 nm coherent radiation generated by a XeCl excimer laser. We compared these effects with those produced by a conventional far-UV254 nm Germicidal Lamp. Compared to the Germicidal Lamp, the excimer laser was able to better discriminate the different recA-defective strains on the basis of their UV-radiation sensitivity, which was progressively higher in the strains with the alleles in the order recA1, recA56 and recA13. This finding was...