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Mohammad Anis - One of the best experts on this subject based on the ideXlab platform.
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Encapsulation of nodal segments of Allamanda cathartica for short-term storage and Germplasm Exchange
Plant Cell Tissue and Organ Culture (PCTOC), 2021Co-Authors: Mehrun Nisha Khanam, Saad Bin Javed, Naseem Ahmad, Mohammad AnisAbstract:Synseed technology enhances the efforts of micropropagation and helps in the maintenance of rare, endangered and elite genotypes. Allamanda cathartica possesses high global market value due to the presence of phytoconstituent in leaves and roots of the plant. Plant has ornamental properties for its attractive funnel shaped yellow coloured flowers. Genetic stability of the regenerated plantlets were analyzed using PCR-based molecular markers. Synthetic seed has the potential to be used as a cost-effective tool for propagation and storage of Germplasm. In the present study, synthetic seeds were produced by encapsulating nodal segments of Allamanda cathartica in calcium alginate gel. The best gel complex was achieved using 3% sodium alginate and 100 mM calcium chloride (CaCl_2·2H_2O). Maximum conversion response of encapsulated nodal segments into plantlets was obtained on Murashige and Skoog (MS) medium with 3.0 μM meta-Topolin (mT) and 0.5 μM alpha-naphthalene acetic acid (NAA). Encapsulated nodal segments could be stored at low temperature (4 °C) up to 6 weeks with a survival frequency of 85%. The regenerated shoots rooted on half-strength MS medium containing 0.5 µM indole-3-butyric acid (IBA). Plantlets with adequate root and shoot systems were hardened off successfully with 90% success rate. Inter Simple Sequence Repeat (ISSR) markers were used to evaluate genetic stability among synthetic seed derived plantlets. The repeated high regeneration of plants from alginate-coated axillary buds and apparently good viability of the synseeds after four weeks of storage indicate that this is a viable strategy for the Exchange of A. cathartica genetic resources.
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nutrient alginate encapsulation of nodal segments of althaea officinalis l for short term conservation and Germplasm Exchange
Plant Biosystems, 2018Co-Authors: Mohammad Anis, Abdulrahman A Alatar, Altaf Ahmad, Afshan NaazAbstract:AbstractIn the present study, an alternate method for Germplasm storage in the form of artificial seeds was standardized via nodal explants excised from in vitro proliferated shoots. The explants were encapsulated using sodium alginate and calcium chloride as gelling matrix. For development of root along with shoot, excised nodal segments were pretreated with ½ MS medium along with 20 μM IBA for 24 h and encapsulation was carried thereafter. Combination of 3% sodium alginate augmented with 100 mM CaCl2.2H2O was found appropriate for the formation of clear and uniform beads and subsequent conversion of encapsulated nodal segments into plantlets. Maximum (66%) encapsulated nodal segments were converted into plantlets on MS medium supplemented with 7.5 μM BA and 0.5 μM NAA after eight weeks. Regeneration frequency of auxin-pretreated encapsulated and non-encapsulated nodal segments (stored at 4 oC) was evaluated at different storage time (0 to 6 weeks). After four weeks of storage, encapsulated propagules ex...
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nutrient alginate encapsulation of nodal segments of althaea officinalis l for short term conservation and Germplasm Exchange
Plant Biosystems, 2018Co-Authors: Ruphi Naz, Mohammad Anis, Abdulrahman A Alatar, Altaf Ahmad, Afshan NaazAbstract:In the present study, an alternate method for Germplasm storage in the form of artificial seeds was standardized via nodal explants excised from in vitro proliferated shoots. The explants were enca...
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Encapsulation technology for short-term storage and Germplasm Exchange of Vitex trifolia L.
Rendiconti Lincei, 2015Co-Authors: Md Rafique Ahmed, Mohammad Anis, Hashim A. Al-ettaAbstract:The current study reports the encapsulation of nodal segment of V. trifolia L. excised from 2-month-old in vitro-raised cultures for short-term conservation and propagation. The encapsulation of nodal segments was significantly affected by the concentrations of sodium alginate (Na alginate) and calcium chloride (CaCl_2 2H_2O). The best gel complex using 3 % sodium alginate and 100 mM CaCl_2 2H_2O was found most suitable for the production of ideal Ca alginate beads. Maximum percent conversion response (84.9 %) was recorded on Murashige and Skoog (MS) basal medium supplemented with 5.0 µM 6-benzyladenine (BA), 0.5 µM α-naphthalene acetic acid (NAA) after 6 weeks of culture. The encapsulated nodal segments could be stored at 4 °C up to 8 weeks with 42.5 % regeneration efficiency. Plantlets obtained were rooted best on full-strength MS medium containing 0.5 µM α-naphthalene acetic acid (NAA) for the production of complete plantlets. The regenerated plantlets were successfully hardened and established in field where they grew well without any detectable variation with a survival rate of 92 %. The high frequency of plant re-growth from alginate-coated nodal segments coupled with high viability percentage after 4 weeks of storage is highly encouraging for the Exchange of V. trifolia genetic resources.
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synseed conception for short term storage Germplasm Exchange and potentialities of regeneration genetically stable plantlets of desert date tree balanites aegyptiaca del
Agroforestry Systems, 2014Co-Authors: Ankita Varshney, Mohammad AnisAbstract:Synthetic seed has become a proficient tool that facilitates conservation as well as mass propagation of elite plant species by encapsulating somatic embryo or meristem tissue. Inclusion of meristematic tissues instead of somatic embryos hastens widespread utilization of this technology in recent years. Synseeds offer short term conservation of Germplasms, provide readily available tissue source for easy mass propagation where each synseeds can virtually act as zygotic seeds giving rise to plantlets. This is the first report on synseed production following the confirmation of the genetic homogeneity in regenerated plantlets of Balanites aegyptiaca Del. (L.) using ISSR marker system. In this study, nodal segments, excised from in vitro proliferated shoot cultures developed from mother plant, were encapsulated in calcium alginate beads and the finest gel complexation was achieved using 3 % sodium alginate and 100 mM CaCl2·2H2O. Maximum percent response (80 %) for conversion of encapsulated nodal segments into plantlets was obtained on Murashige and Skoog (MS) medium containing 12.5 μM benzyladenine and 1.0 μM α-naphthalene acetic acid. Encapsulated nodal segments could be stored at low temperature (4 °C) up to 4 weeks with a survival frequency of 82 %. The regenerated shoots were rooted on half strength MS medium augmented with 1.0 μM indole-3-butyric acid. Well-developed plantlets regenerated from encapsulated nodal segments were acclimatized successfully with 90 % survival frequency. Fingerprinting profiles of the regenerated plantlets derived from synseeds and the donor plant were generated using a total of 20 ISSR primers, of which 14 primers produced distinct, reproducible amplified products. A total of 158 scorable bands were obtained from the complete amalgamation of primers and plantlets and 98.7 % bands were monomorphic across the plantlets which indicate that this micropropagated line derived from synseed is genetically stable and demonstrates the reliability of our protocol for short term conservation, Germplasm Exchange and distribution of identical plants.
Afshan Naaz - One of the best experts on this subject based on the ideXlab platform.
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nutrient alginate encapsulation of nodal segments of althaea officinalis l for short term conservation and Germplasm Exchange
Plant Biosystems, 2018Co-Authors: Mohammad Anis, Abdulrahman A Alatar, Altaf Ahmad, Afshan NaazAbstract:AbstractIn the present study, an alternate method for Germplasm storage in the form of artificial seeds was standardized via nodal explants excised from in vitro proliferated shoots. The explants were encapsulated using sodium alginate and calcium chloride as gelling matrix. For development of root along with shoot, excised nodal segments were pretreated with ½ MS medium along with 20 μM IBA for 24 h and encapsulation was carried thereafter. Combination of 3% sodium alginate augmented with 100 mM CaCl2.2H2O was found appropriate for the formation of clear and uniform beads and subsequent conversion of encapsulated nodal segments into plantlets. Maximum (66%) encapsulated nodal segments were converted into plantlets on MS medium supplemented with 7.5 μM BA and 0.5 μM NAA after eight weeks. Regeneration frequency of auxin-pretreated encapsulated and non-encapsulated nodal segments (stored at 4 oC) was evaluated at different storage time (0 to 6 weeks). After four weeks of storage, encapsulated propagules ex...
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nutrient alginate encapsulation of nodal segments of althaea officinalis l for short term conservation and Germplasm Exchange
Plant Biosystems, 2018Co-Authors: Ruphi Naz, Mohammad Anis, Abdulrahman A Alatar, Altaf Ahmad, Afshan NaazAbstract:In the present study, an alternate method for Germplasm storage in the form of artificial seeds was standardized via nodal explants excised from in vitro proliferated shoots. The explants were enca...
Kevin P. Smith - One of the best experts on this subject based on the ideXlab platform.
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A genome-wide association study of malting quality across eight U.S. barley breeding programs.
TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2015Co-Authors: Mohsen Mohammadi, Tom Blake, Allen D. Budde, Shiaoman Chao, Patrick M. Hayes, Richard D. Horsley, D. E. Obert, Steven E. Ullrich, Kevin P. SmithAbstract:Key message We report malt quality QTLs relevant to breeding with greater precision than previous mapping studies. The distribution of favorable alleles suggests strategies for marker-assisted breeding and Germplasm Exchange.
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a genome wide association study of malting quality across eight u s barley breeding programs
Theoretical and Applied Genetics, 2015Co-Authors: Mohsen Mohammadi, Tom Blake, Allen D. Budde, Shiaoman Chao, Patrick M. Hayes, Richard D. Horsley, D. E. Obert, Steven E. Ullrich, Kevin P. SmithAbstract:We report malt quality QTLs relevant to breeding with greater precision than previous mapping studies. The distribution of favorable alleles suggests strategies for marker-assisted breeding and Germplasm Exchange. This study leverages the breeding data of 1,862 barley breeding lines evaluated in 97 field trials for genome-wide association study of malting quality traits in barley. The mapping panel consisted of six-row and two-row advanced breeding lines from eight breeding populations established at six public breeding programs across the United States. A total of 4,976 grain samples were subjected to micro-malting analysis and mapping of nine quality traits was conducted with 3,072 SNP markers distributed throughout the genome. Association mapping was performed for individual breeding populations and for combined six-row and two-row populations. Only 16 % of the QTL we report here had been detected in prior bi-parental mapping studies. Comparison of the analyses of the combined two-row and six-row panels identified only two QTL regions that were common to both. In total, 108 and 107 significant marker-trait associations were identified in all six-row and all two-row breeding programs, respectively. A total of 102 and 65 marker-trait associations were specific to individual six-row and two-row breeding programs, respectively indicating that most marker-trait associations were breeding population specific. Combining datasets from different breeding program resulted in both the loss of some QTL that were apparent in the analyses of individual programs and the discovery of new QTL not identified in individual programs. This suggests that simply increasing sample size by pooling samples with different breeding history does not necessarily increase the power to detect associations. The genetic architecture of malting quality and the distribution of favorable alleles suggest strategies for marker-assisted selection and Germplasm Exchange.
Manoj K. Rai - One of the best experts on this subject based on the ideXlab platform.
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alginate encapsulation of nodal segments for propagation short term conservation and Germplasm Exchange and distribution of eclipta alba l
Acta Physiologiae Plantarum, 2010Co-Authors: Pooja Asthana, Manoj K. Rai, Shashi Kant Singh, Lingaraj SahooAbstract:Nodal segments obtained from in vitro proliferated shoots of Eclipta alba (L.) Hassk, were encapsulated in calcium alginate beads for large-scale clonal propagation, short-term conservation and Germplasm Exchange and distribution. The best gel complexation was achieved using 3% sodium alginate and 100 mM CaCl2·2H2O. Maximum percent response (100%) for conversion of encapsulated nodal segments into plantlets was obtained on 0.7% agar-solidified full-strength MS medium containing 0.88 μM BAP. Encapsulated nodal segments could be stored at low temperature (4°C) up to 60 days with a survival frequency of 51.2%. The well-developed plantlets regenerated from encapsulated nodal segments were hardened-off successfully with 90% survival frequency.
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Alginate-encapsulation of shoot tips of jojoba [Simmondsia chinensis (Link) Schneider] for Germplasm Exchange and distribution
Physiology and Molecular Biology of Plants, 2010Co-Authors: Sunil Kumar, Narender Singh, Manoj K. Rai, Manisha MangalAbstract:Shoot tips excised from in vitro proliferated shoots derived from nodal explants of jojoba [Simmondsia chinensis (Link) Schneider] were encapsulated in calcium alginate beads for Germplasm Exchange and distribution. A gelling matrix of 3 % sodium alginate and 100 mM calcium chloride was found most suitable for formation of ideal calcium alginate beads. Best response for shoot sprouting from encapsulated shoot tips was recorded on 0.8 % agar-solidified full-strength MS medium. Rooting was induced upon transfer of sprouted shoots to 0.8 % agar-solidified MS medium containing 1 mg l(-1) IBA. About 70 % of encapsulated shoot tips were rooted and converted into plantlets. Plants regenerated from encapsulated shoot tips were acclimatized successfully. The present encapsulation approach could also be applied as an alternative method of propagation of desirable elite genotype of jojoba.
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encapsulation of shoot tips of guava psidium guajava l for short term storage and Germplasm Exchange
Scientia Horticulturae, 2008Co-Authors: Manoj K. Rai, V. S. Jaiswal, U. JaiswalAbstract:Abstract Shoot tips obtained from in vitro grown plantlets of guava (Psidium guajava L.) were encapsulated in calcium alginate beads for short-term storage and Germplasm Exchange. A gelling matrix of 3% sodium alginate and 100 mM calcium chloride was found most suitable for formation of ideal calcium alginate beads. Maximum percent response for conversion of encapsulated shoot tips into plantlets was obtained on growth regulator free full strength liquid MS medium. The regrowth ability of encapsulated shoot tips was affected by medium strength and sucrose concentrations in the medium. Encapsulated shoot tips could be stored at low temperature (4 °C) up to 30 days with a survival frequency of 25%. After 60 days of storage under minimal growth conditions (sucrose lacking medium), about 75% encapsulated shoot tips were converted into plantlets when subcultured on 3% sucrose containing medium. Plantlets regenerated from encapsulated shoot tips were acclimatized successfully.
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alginate encapsulation of nodal segments of guava psidium guajava l for Germplasm Exchange and distribution
Journal of Horticultural Science & Biotechnology, 2008Co-Authors: Manoj K. Rai, V. S. Jaiswal, Uma JaiswalAbstract:SummaryNodal segments obtained from in vitro grown plantlets of guava (Psidium guajava L.) were encapsulated in calcium alginate beads for Germplasm Exchange and distribution. The best gel complex for encapsulation of nodal segments was achieved using 3% (w/v) sodium alginate and 100 mM calcium chloride.The maximum conversion of encapsulated nodal segments into plantlets was obtained on growth regulator-free, full-strength, liquid Murashige and Skoog medium after a pulse treatment with 4.4 µM BAP (6-benzylaminopurine) for 1 week prior to encapsulation. Plants regenerated from encapsulated nodal segments were acclimatised successfully. The present encapsulation approach may also be useful in large-scale propagation of desirable elite genotypes and genetically modified plants.
Anwar Shahzad - One of the best experts on this subject based on the ideXlab platform.
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high frequency clonal propagation encapsulation of nodal segments for short term storage and Germplasm Exchange of ficus carica l
Trees-structure and Function, 2015Co-Authors: Shiwali Sharma, Anwar Shahzad, Sana Mahmood, Taiba SaeedAbstract:The study describes an efficient regeneration system and encapsulation of nodal segments in F. carica accompanied with frequent root formation in microshoots under the influence of salicylic acid. The present study describes an improved protocol for clonal propagation and a short-term conservation strategy using an encapsulation technology for Ficus carica. Nodal segments were more responsive than shoot tips for shoot multiplication. Among the cytokinins tested, Murashige and Skoog’s (MS) medium augmented with 7.5 µM 6-benzyladenine (BA) was the best, but the leaves exhibited a rudimentary structure. Cytokinin–auxin combinations enhanced the multiplication rate; but leaf growth did not improve. However, the supplementation of 50 µM adenine sulphate (Ads) to MS + 7.5 µM BA + 0.5 µM α-naphthalene acetic acid (NAA) significantly enhanced mean shoot number per explant and overall growth of shoots and induced a maximum of 26.8 shoots per nodal segment and 15 shoots per shoot tip with mean shoot length of 8 and 4.3 cm, respectively, after 4 weeks of culture. Microshoots rooted on half-strength MS medium containing 2.5 µM salicylic acid (SA) either in semi-solid (mean of 4.6 roots per shoot) or liquid medium (mean of 5 roots per shoot). A gelling matrix of 4 % sodium alginate (Na-alginate) and 100 mM calcium chloride (CaCl2·2H2O) was used for the encapsulation of nodal segments. Maximum shoot growth (95.6 %) was recorded on MS medium supplemented with 7.5 µM BA + 0.5 µM NAA + 50 µM Ads. Rooting was induced upon transfer of sprouted microshoots to semi-solid half-strength MS medium supplemented with 2.5 µM SA. To examine the retention of encapsulated and non-encapsulated nodal segments’ viability, a low-temperature storage (4 °C) experiment was carried out. Encapsulated nodal segments stored at 4 °C for 1–6 weeks sprouted at variable frequencies in successive weeks of transfer. Plantlets were acclimatized and established in field where they grew without any detectable variations after 6 months of transfer.
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encapsulation of nodal segments of cassia angustifolia vahl for short term storage and Germplasm Exchange
Acta Physiologiae Plantarum, 2014Co-Authors: Shahina Parveen, Anwar ShahzadAbstract:The present study described the encapsulation of nodal segments of Cassia angustifolia Vahl. excised from 1-month-old in vitro raised cultures for short-term conservation and propagation. Various concentrations and combinations of gelling matrix (sodium alginate) and complexing agents (calcium chloride) were tested to prepare uniform beads. The ideal beads were obtained through a combination of 3 % sodium alginate and 100 mM calcium chloride. The maximum conversion response (94 %) of encapsulated beads was obtained in Murashige and Skoog’s medium (MS medium) supplemented with 2.5 μM benzyladenine (BA) and 0.4 μM α-naphthalene acetic acid (NAA) after 6 weeks of culture. The encapsulated and non-encapsulated nodal segments were also stored at 4 °C for different time periods (0, 1, 2, 4, 6 and 8 weeks). The regenerated microshoots were best rooted in optimized rooting medium that comprised half-strength MS + 1.0 μM indole-3-butyric acid (IBA) + 5.0 μM phloroglucinol (PG) for the production of complete plantlets. The regenerated plantlets were successfully hardened and acclimatized in natural conditions with 70 % survival rate.
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synseed production in spilanthes mauritiana dc for short term storage and Germplasm Exchange
British Biotechnology Journal, 2014Co-Authors: Shiwali Sharma, Anwar ShahzadAbstract:Aims: The present study provides an efficient protocol for short-term storage and Germplasm Exchange of a potent medicinal herb, Spilanthes mauritiana using encapsulated nodal segments. Study Design: For in vitro conversion of synseeds, 5 beads were placed in each flask having Murashige and Skoog (MS) medium supplemented with different combinations of plant growth regulators (PGRs). While for ex vitro conversion, 5 synseeds per thermocol cups having different planting substrates were directly sown. The data for each experiment were collected after 6 weeks. All the experiments were conducted with a minimum of 20 replicates per treatment and each experiment was repeated thrice. Place and Duration of Study: Plant Biotechnology Lab, Department of Botany, AMU, July 2012 to November 2013. Methodology: Concentration of two different manufacture grade of Na-alginate (purchased from Central Drug House and Loba Chemie) were compared for the production of ideal synseeds. Conversion of synseeds was tested under in vitro and ex vitro conditions. A low temperature storage (4oC) experiment was also carried out to understand the explants’ ability to revive physiological activity leading to plantlet development. Original Research Article British Biotechnology Journal, 4(6): 696-707, 2014 697 Results: A gelling matrix of 4% Na-alginate (CDH) or 3% (Loba Chemie) with 100 mM calcium chloride (CaCl2∙2H2O) was found most suitable for the production of ideal Caalginate bead. However, CDH grade Na-alginate (74.4% conversion) was found to be better than Loba Chemie (62% conversion) in terms of in vitro conversion of synseeds into complete plantlets when cultured on MS basal medium. Supplementation of Plant Growth Regulators (PGRs) to the MS basal medium further enhanced the conversion frequency of the synseeds. Maximum conversion (83%) was recorded on MS basal medium supplemented with 5.0 μM 6-benzyladenine (BA) and 0.5 μM indole-3-acetic acid (IAA). Synseeds, stored at 4oC for 1-8 weeks showed successful sprouting with variable percent in successive weeks of transfer, followed by development into complete plantlets when returned to regeneration medium. Ex vitro conversion of synseeds also occurred when synseeds were sown directly into Soilrite moistened with quarter-strength MS salts. Plantlets regenerated from non-stored and stored synseeds were successfully hardened, acclimatized and established in soil with a success of 90%. While plantlets recovered from direct sowing of synseeds exhibited 80% survivability. Conclusion: Being small in size, synseeds provide an effective tool for conservation, storage and Exchange of this valuable medicinal plant species, potentially overcoming many of the difficulties associated with long-distance transport of plant Germplasm.