The Experts below are selected from a list of 327 Experts worldwide ranked by ideXlab platform
Staffan G Svard - One of the best experts on this subject based on the ideXlab platform.
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A chromosome-scale reference genome for Giardia intestinalis WB
Scientific Data, 2020Co-Authors: Feifei Xu, Staffan G SvardAbstract:Giardia intestinalis is a protist causing diarrhea in humans. The first G. intestinalis genome, from the WB isolate, was published more than ten years ago, and has been widely used as the reference ...
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cleavage specificity of recombinant Giardia intestinalis cysteine proteases degradation of immunoglobulins and defensins
Molecular and Biochemical Parasitology, 2019Co-Authors: Zhirong Fu, Lars Hellman, Staffan G SvardAbstract:Giardia intestinalis is a protozoan parasite and the causative agent of Giardiasis, a common diarrheal disease. Cysteine protease (CP) activities have been suggested to be involved in Giardia's pat ...
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secreted Giardia intestinalis cysteine proteases disrupt intestinal epithelial cell junctional complexes and degrade chemokines
Virulence, 2018Co-Authors: Showgy Y Maayeh, Lars Hellman, Dimitra Peirasmaki, Britta Lundstromstadelmann, Staffan G SvardAbstract:Giardiasis is a common diarrheal disease caused by the protozoan parasite Giardia intestinalis. Cysteine proteases (CPs) are acknowledged as virulence factors in Giardia but their specific role in ...
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responses of the differentiated intestinal epithelial cell line caco 2 to infection with the Giardia intestinalis gs isolate
Frontiers in Cellular and Infection Microbiology, 2018Co-Authors: Showgy Y Maayeh, Livia Knorr, Karin Skold, Alexandra L Garnham, Brendan R E Ansell, Staffan G SvardAbstract:Giardia intestinalis is a parasitic protist that causes diarrhea in humans, affecting mainly children of the developing world, elderly and immunocompromised individuals. Humans are infected by two ...
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comparative genomic analyses of freshly isolated Giardia intestinalis assemblage a isolates
BMC Genomics, 2015Co-Authors: Johan Ankarklev, Jon Jerlstromhultqvist, Oscar Franzen, Jan Andersson, Bjorn Andersson, Dimitra Peirasmaki, Marianne Lebbad, Staffan G SvardAbstract:Background: The diarrhea-causing protozoan Giardia intestinalis makes up a species complex of eight different assemblages (A-H), where assemblage A and B infect humans. Comparative whole-genome ana ...
Showgy Y Maayeh - One of the best experts on this subject based on the ideXlab platform.
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secreted Giardia intestinalis cysteine proteases disrupt intestinal epithelial cell junctional complexes and degrade chemokines
Virulence, 2018Co-Authors: Showgy Y Maayeh, Lars Hellman, Dimitra Peirasmaki, Britta Lundstromstadelmann, Staffan G SvardAbstract:Giardiasis is a common diarrheal disease caused by the protozoan parasite Giardia intestinalis. Cysteine proteases (CPs) are acknowledged as virulence factors in Giardia but their specific role in ...
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responses of the differentiated intestinal epithelial cell line caco 2 to infection with the Giardia intestinalis gs isolate
Frontiers in Cellular and Infection Microbiology, 2018Co-Authors: Showgy Y Maayeh, Livia Knorr, Karin Skold, Alexandra L Garnham, Brendan R E Ansell, Staffan G SvardAbstract:Giardia intestinalis is a parasitic protist that causes diarrhea in humans, affecting mainly children of the developing world, elderly and immunocompromised individuals. Humans are infected by two ...
Bjorn Andersson - One of the best experts on this subject based on the ideXlab platform.
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comparative genomic analyses of freshly isolated Giardia intestinalis assemblage a isolates
BMC Genomics, 2015Co-Authors: Johan Ankarklev, Jon Jerlstromhultqvist, Oscar Franzen, Jan Andersson, Bjorn Andersson, Dimitra Peirasmaki, Marianne Lebbad, Staffan G SvardAbstract:Background: The diarrhea-causing protozoan Giardia intestinalis makes up a species complex of eight different assemblages (A-H), where assemblage A and B infect humans. Comparative whole-genome ana ...
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genome analysis and comparative genomics of a Giardia intestinalis assemblage e isolate
BMC Genomics, 2010Co-Authors: Jon Jerlstromhultqvist, Johan Ankarklev, Oscar Franzen, Feifei Xu, Eva Nohýnkova, Jan Andersson, Staffan G Svard, Bjorn AnderssonAbstract:Giardia intestinalis is a protozoan parasite that causes diarrhea in a wide range of mammalian species. To further understand the genetic diversity between the Giardia intestinalis species, we have performed genome sequencing and analysis of a wild-type Giardia intestinalis sample from the assemblage E group, isolated from a pig. We identified 5012 protein coding genes, the majority of which are conserved compared to the previously sequenced genomes of the WB and GS strains in terms of microsynteny and sequence identity. Despite this, there is an unexpectedly large number of chromosomal rearrangements and several smaller structural changes that are present in all chromosomes. Novel members of the VSP, NEK Kinase and HCMP gene families were identified, which may reveal possible mechanisms for host specificity and new avenues for antigenic variation. We used comparative genomics of the three diverse Giardia intestinalis isolates P15, GS and WB to define a core proteome for this species complex and to identify lineage-specific genes. Extensive analyses of polymorphisms in the core proteome of Giardia revealed differential rates of divergence among cellular processes. Our results indicate that despite a well conserved core of genes there is significant genome variation between Giardia isolates, both in terms of gene content, gene polymorphisms, structural chromosomal variations and surface molecule repertoires. This study improves the annotation of the Giardia genomes and enables the identification of functionally important variation.
Edith Brotlaroche - One of the best experts on this subject based on the ideXlab platform.
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asymptomatic Giardia intestinalis infection and roux en y gastric bypass
Surgery for Obesity and Related Diseases, 2015Co-Authors: Laurent Genser, Christine Poitoubernert, Edith BrotlarocheAbstract:Giardiasis is one of the most common parasitic infections of the human intestine worldwide, and ranges from 3-7% in western countries to 100% in some populations [1]. Giardia intestinalis (i.e Duodenalis or Lamblia) is a flagellated, binucleated protozoan parasite that infects the upper intestinal tract in many mammalian hosts. Giardiasis can present with a broad range of clinical manifestations, from being asymptomatic to acute or chronic nonspecific gastro-intestinal symptoms associated with malnutrition [2]. However little is known regarding Giardiasis in human obesity. [...]
Johan Ankarklev - One of the best experts on this subject based on the ideXlab platform.
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Genomic variation within Giardia intestinalis assemblage A isolates
2020Co-Authors: Johan AnkarklevAbstract:Background: The diarrhea-causing protozoan Giardia intestinalis makes up a species complex of eight different assemblages (A-H), where assemblage A and B infect humans. We have performed whole geno ...
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Inter and Intra-Assemblage Characterizations of Giardia intestinalis: from clinic to genome
2020Co-Authors: Johan AnkarklevAbstract:The protozoan parasite Giardia intestinalis (syn. G. lamblia, G. duodenalis) is one of the most common causes of diarrheal disease throughout the world, where an estimated 500 million people are in ...
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comparative genomic analyses of freshly isolated Giardia intestinalis assemblage a isolates
BMC Genomics, 2015Co-Authors: Johan Ankarklev, Jon Jerlstromhultqvist, Oscar Franzen, Jan Andersson, Bjorn Andersson, Dimitra Peirasmaki, Marianne Lebbad, Staffan G SvardAbstract:Background: The diarrhea-causing protozoan Giardia intestinalis makes up a species complex of eight different assemblages (A-H), where assemblage A and B infect humans. Comparative whole-genome ana ...
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common coinfections of Giardia intestinalis and helicobacter pylori in non symptomatic ugandan children
PLOS Neglected Tropical Diseases, 2012Co-Authors: Johan Ankarklev, Jan Andersson, Marianne Lebbad, Elin Hestvik, Johan Lindh, Deogratias H Kaddumulindwa, Thorkild Tylleskar, James K Tumwine, Staffan G SvardAbstract:Background The protozoan parasite Giardia intestinalis and the pathogenic bacterium Helicobacter pylori are well known for their high prevalences in human hosts worldwide. The prevalence of both organisms is known to peak in densely populated, low resource settings and children are infected early in life. Different Giardia genotypes/assemblages have been associated with different symptoms and H. pylori with induction of cancer. Despite this, not much data are available from sub-Saharan Africa with regards to the prevalence of different G. intestinalis assemblages and their potential association with H. pylori infections.
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genome analysis and comparative genomics of a Giardia intestinalis assemblage e isolate
BMC Genomics, 2010Co-Authors: Jon Jerlstromhultqvist, Johan Ankarklev, Oscar Franzen, Feifei Xu, Eva Nohýnkova, Jan Andersson, Staffan G Svard, Bjorn AnderssonAbstract:Giardia intestinalis is a protozoan parasite that causes diarrhea in a wide range of mammalian species. To further understand the genetic diversity between the Giardia intestinalis species, we have performed genome sequencing and analysis of a wild-type Giardia intestinalis sample from the assemblage E group, isolated from a pig. We identified 5012 protein coding genes, the majority of which are conserved compared to the previously sequenced genomes of the WB and GS strains in terms of microsynteny and sequence identity. Despite this, there is an unexpectedly large number of chromosomal rearrangements and several smaller structural changes that are present in all chromosomes. Novel members of the VSP, NEK Kinase and HCMP gene families were identified, which may reveal possible mechanisms for host specificity and new avenues for antigenic variation. We used comparative genomics of the three diverse Giardia intestinalis isolates P15, GS and WB to define a core proteome for this species complex and to identify lineage-specific genes. Extensive analyses of polymorphisms in the core proteome of Giardia revealed differential rates of divergence among cellular processes. Our results indicate that despite a well conserved core of genes there is significant genome variation between Giardia isolates, both in terms of gene content, gene polymorphisms, structural chromosomal variations and surface molecule repertoires. This study improves the annotation of the Giardia genomes and enables the identification of functionally important variation.