The Experts below are selected from a list of 2217 Experts worldwide ranked by ideXlab platform
Peng Yang - One of the best experts on this subject based on the ideXlab platform.
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Anti-breast cancer triterpenoid saponins from the thorns of Gleditsia sinensis.
Natural product research, 2018Co-Authors: Honglei Zhou, Xiao Wang, Peng YangAbstract:One new triterpenoid saponin (1), as well as six known ones (2–7), were isolated from the ethanol extract of the thorns of Gleditsia sinensis. Their structures were elucidated by extensive spectros...
Sungkwon Moon - One of the best experts on this subject based on the ideXlab platform.
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Gleditsia sinensis thorn extract inhibits proliferation and tnf α induced mmp 9 expression in vascular smooth muscle cells
The American Journal of Chinese Medicine, 2012Co-Authors: Sejung Lee, Wunjae Kim, Sungsoo Park, Sungkwon MoonAbstract:The thorns of Gleditsia sinensis, which are extensively used as a medicinal herb in Asian countries, have been reported to exert various pharmacological effects. However, the anti-atherogenic effect of Gleditsia sinensis thorns has never been investigated. In the present study, we investigated the role and effect of the ethanol extract of Gleditsia sinensis thorns (EEGS) on cultured vascular smooth muscle cells (VSMC). Treatment of VSMC with EEGS led to a significant decrease in cell growth by arresting cells in the G2/M-phase of the cell cycle, which was associated with up-regulated p21WAF1 levels and suppression of G2/M cell cycle regulators, cyclinB1, Cdc2 and Cdc25c. In addition, EEGS treatment led to the induction of extracellular signal-regulated kinase1/2 (ERK1/2), p38 MAPK, and JNK (c-Jun N-terminal kinases) activation. EEGS-induced p21WAF1 expression was blocked by treatment with the p38 MAPK-specific inhibitor SB203580. SB203580 also markedly recovered the inhibition of cell growth and decrease in cell cycle proteins in EEGS-treated VSMC. Moreover, EEGS inhibited matrix metalloproteinase-9 (MMP-9) expression induced by tumor necrosis factor-α (TNF-α) in VSMC. Finally, an electrophoresis mobility shift assay demonstrated that EEGS suppressed expression of transcription factor, nuclear factor kappaB (NF-κB) and activator protein-1 (AP-1), which are essential cis-elements for the MMP-9 promoter in TNF-α-treated VSMC. These results demonstrate that EEGS exerts a potent inhibitory effect on cell proliferation and MMP-9 expression in VSMC. These unexpected novel findings represent theoretical data for the preventive and therapeutic use of EEGS for the treatment of atherosclerosis disease.
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inhibitory effects of the ethanol extract of Gleditsia sinensis thorns on human colon cancer hct116 cells in vitro and in vivo
Oncology Reports, 2009Co-Authors: Sejung Lee, Younghwa Cho, Heejong Kim, Keerang Park, Sungkyu Park, Wunjae Kim, Sungkwon MoonAbstract:The thorns of Gleditsia sinensis have traditionally been used in the treatment of several diseases, which includes their use as anti-tumor agents, but there has been no scientific evidence of this anti-tumor effect. However, the present study has identified a novel mechanism for the anti-tumor effect of Gleditsia sinensis thorns in the treatment of colon cancer. Treatment with the ethanol extract of Gleditsia sinensis thorns (EEGS) resulted in significant growth inhibition together with G2/M-phase cell cycle arrest at a dose of 600 microg/ml (IC50) in HCT116 cells. In addition, treatment with EEGS induced p27 expression and down-regulated expression of cyclins and cyclin-dependent kinases. Moreover, EEGS treatment induced phosphorylation of extracellular signal-regulated kinases (ERK), p38 MAP kinase and JNK (c-Jun N-terminal kinases). Among the pathways examined, only PD98059 (ERK-specific inhibitor) abolished EEGS-dependent p27 expression. Similarly, suppression of ERK function reversed EEGS-mediated cell proliferation inhibition and decreased cell cycle proteins. In addition, tumor necrosis factor-alpha (TNF-alpha)-induced matrix metalloproteinase-9 (MMP-9) expression was inhibited by EEGS treatment via decreased transcriptional activity of both activator protein-1 (AP-1) and nuclear factor-kappaB. Finally, EEGS treatment significantly reduced tumor sizes in HCT116 cell-xenografted tumor tissues, which was associated with the changed levels of ERK phosphorylation, p27 and MMP-9 expression. Overall, these results have identified a novel molecular mechanism for EEGS in the treatment of colon cancer and might provide a theoretical basis for the potential therapeutic use of EEGS in the treatment of malignancies.
Zhizhen Zhang - One of the best experts on this subject based on the ideXlab platform.
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Quantitive analysis of Gleditsia saponins in the fruits of Gleditsia sinensis Lam. by high performance liquid chromatography.
Journal of pharmaceutical and biomedical analysis, 2012Co-Authors: Xiao-yuan Lian, Zhizhen ZhangAbstract:A high performance liquid chromatography method was developed for simultaneous quantification of 29 ingredients occurring as a very complex mixture in the Gleditsia fruits. The analysis was performed on an ODS-2 Hypersil column (250 mm × 4.6 mm, 5 μm) with a binary gradient mobile phase of water and acetonitrile both containing 0.1% acetic acid. The method was validated in terms of linearity, sensitivity, stability, precision, and accuracy. It was found that this method had linearity with R² at 0.99889-0.99997 in the test range of 1.0-24.0 μg. The limit of detection (LOD) and limit of quantification (LOQ) for 16 tested reference saponins were 0.24-0.39 μg and 1.0-1.2 μg, respectively. The relative standard deviations (RSDs %) for intra-day and inter-day repeatability were not more than 3.11% and 4.02%, respectively. The analyzed samples were stable for at least 48 h. The spike recoveries for eight analyzed saponins were 99.68-102.17%. The established HPLC analytic method was successfully used to determine the concentrations of 29 compounds including 19 Gleditsia saponins and ten unidentified ingredients in eight commercial Gleditsia fruits from different sources. The results from this study suggested that this newly developed HPLC method could be used for qualitative and quantitative analysis of the saponins in the Gleditsia fruits and the Gleditsia extracts that used for animal study and other purpose.
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Triterpenoidal saponins from Gleditsia sinensis.
Phytochemistry, 1999Co-Authors: Zhizhen Zhang, Kazuo Koike, Zhonghua Jia, Tamotsu Nikaido, De-an Guo, Junhua ZhengAbstract:Six bisdesmosidic triterpenoidal saponins, gleditsiosides H-K and Gleditsia saponins C' and E', were isolated from the anomalous fruits of Gleditsia sinensis. Their structures were established by a combination of extensive NMR (DEPT, DQF-COSY, HETCOR, HOHAHA, HMBC and ROESY) studies and chemical degradation.
Sejung Lee - One of the best experts on this subject based on the ideXlab platform.
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Gleditsia sinensis thorn extract inhibits proliferation and tnf α induced mmp 9 expression in vascular smooth muscle cells
The American Journal of Chinese Medicine, 2012Co-Authors: Sejung Lee, Wunjae Kim, Sungsoo Park, Sungkwon MoonAbstract:The thorns of Gleditsia sinensis, which are extensively used as a medicinal herb in Asian countries, have been reported to exert various pharmacological effects. However, the anti-atherogenic effect of Gleditsia sinensis thorns has never been investigated. In the present study, we investigated the role and effect of the ethanol extract of Gleditsia sinensis thorns (EEGS) on cultured vascular smooth muscle cells (VSMC). Treatment of VSMC with EEGS led to a significant decrease in cell growth by arresting cells in the G2/M-phase of the cell cycle, which was associated with up-regulated p21WAF1 levels and suppression of G2/M cell cycle regulators, cyclinB1, Cdc2 and Cdc25c. In addition, EEGS treatment led to the induction of extracellular signal-regulated kinase1/2 (ERK1/2), p38 MAPK, and JNK (c-Jun N-terminal kinases) activation. EEGS-induced p21WAF1 expression was blocked by treatment with the p38 MAPK-specific inhibitor SB203580. SB203580 also markedly recovered the inhibition of cell growth and decrease in cell cycle proteins in EEGS-treated VSMC. Moreover, EEGS inhibited matrix metalloproteinase-9 (MMP-9) expression induced by tumor necrosis factor-α (TNF-α) in VSMC. Finally, an electrophoresis mobility shift assay demonstrated that EEGS suppressed expression of transcription factor, nuclear factor kappaB (NF-κB) and activator protein-1 (AP-1), which are essential cis-elements for the MMP-9 promoter in TNF-α-treated VSMC. These results demonstrate that EEGS exerts a potent inhibitory effect on cell proliferation and MMP-9 expression in VSMC. These unexpected novel findings represent theoretical data for the preventive and therapeutic use of EEGS for the treatment of atherosclerosis disease.
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inhibitory effects of the ethanol extract of Gleditsia sinensis thorns on human colon cancer hct116 cells in vitro and in vivo
Oncology Reports, 2009Co-Authors: Sejung Lee, Younghwa Cho, Heejong Kim, Keerang Park, Sungkyu Park, Wunjae Kim, Sungkwon MoonAbstract:The thorns of Gleditsia sinensis have traditionally been used in the treatment of several diseases, which includes their use as anti-tumor agents, but there has been no scientific evidence of this anti-tumor effect. However, the present study has identified a novel mechanism for the anti-tumor effect of Gleditsia sinensis thorns in the treatment of colon cancer. Treatment with the ethanol extract of Gleditsia sinensis thorns (EEGS) resulted in significant growth inhibition together with G2/M-phase cell cycle arrest at a dose of 600 microg/ml (IC50) in HCT116 cells. In addition, treatment with EEGS induced p27 expression and down-regulated expression of cyclins and cyclin-dependent kinases. Moreover, EEGS treatment induced phosphorylation of extracellular signal-regulated kinases (ERK), p38 MAP kinase and JNK (c-Jun N-terminal kinases). Among the pathways examined, only PD98059 (ERK-specific inhibitor) abolished EEGS-dependent p27 expression. Similarly, suppression of ERK function reversed EEGS-mediated cell proliferation inhibition and decreased cell cycle proteins. In addition, tumor necrosis factor-alpha (TNF-alpha)-induced matrix metalloproteinase-9 (MMP-9) expression was inhibited by EEGS treatment via decreased transcriptional activity of both activator protein-1 (AP-1) and nuclear factor-kappaB. Finally, EEGS treatment significantly reduced tumor sizes in HCT116 cell-xenografted tumor tissues, which was associated with the changed levels of ERK phosphorylation, p27 and MMP-9 expression. Overall, these results have identified a novel molecular mechanism for EEGS in the treatment of colon cancer and might provide a theoretical basis for the potential therapeutic use of EEGS in the treatment of malignancies.
Yegao Chen - One of the best experts on this subject based on the ideXlab platform.
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A new neolignan from the thorns of Gleditsia japonica var. delavayi.
Natural product research, 2018Co-Authors: Hongmei Zhang, Yong-kang Zhang, Yanxi Wang, Rui Zhan, Yegao ChenAbstract:A new neolignan, 5-(3″-acetoxypropyl)-2-(4′-hydroxy-3′-methoxyphenyl)-7-methoxy-3-methylbenzofuran (1) along with nine analogues were isolated from the thorns of Gleditsia japonica var. delavayi by...