The Experts below are selected from a list of 1566 Experts worldwide ranked by ideXlab platform
Markku Maki - One of the best experts on this subject based on the ideXlab platform.
-
tissue transglutaminase autoAntibody enzyme linked immunosorbent assay in detecting celiac disease
Gastroenterology, 1998Co-Authors: S Sulkanen, Tuula Halttunen, Kaija Laurila, Ilma Rita Korponayszabo, Annikki Sarnesto, Pekka Collin, Kaija-leena Kolho, Erkki Savilahti, Markku MakiAbstract:BACKGROUND & AIMS: Tissue transglutaminase has been reported to be the target for endomysial antibodies in celiac disease. We sought to establish whether immunoglobulin (Ig) A class tissue transglutaminase autoantibodies can be considered specific for celiac disease. METHODS: Serum samples from 136 patients with untreated celiac disease (diagnosed according to the criteria of the European Society for Pediatric Gastroenterology, Hepatology and Nutrition) and 207 disease controls were studied. Enzyme-linked immunosorbent assay (ELISA) and Western blots were performed using calcium-treated and untreated tissue transglutaminase as antigen. Reticulin, endomysial, and mouse monoclonal tissue transglutaminase antibodies were studied by an indirect immunofluorescence method and Gliadin antibodies with ELISA. RESULTS: The calcium-activated tissue transglutaminase autoAntibody ELISA was highly sensitive (129 of 136) and specific (194 of 207) in detecting celiac disease. The new autoAntibody ELISA test correlated well with the endomysial Antibody test. Tissue transglutaminase autoAntibody ELISA showed a clearly better predictive potential than the IgA class Gliadin Antibody ELISA. Immunoblots and ELISA blocking studies showed that calcium is needed for the specific antigen-Antibody reaction to occur. Double immunofluorescence staining in human umbilical cord with sera from patients with celiac disease and with monoclonal tissue transglutaminase antibodies showed complete overlap. CONCLUSIONS: Calcium-activated tissue transglutaminase autoAntibody ELISA is highly accurate in detecting untreated celiac disease. Tissue transglutaminase seems to be the target self-antigen for endomysial antibodies.
-
tissue transglutaminase autoAntibody enzyme linked immunosorbent assay in detecting celiac disease
Gastroenterology, 1998Co-Authors: S Sulkanen, Tuula Halttunen, Kaija Laurila, Ilma Rita Korponayszabo, Annikki Sarnesto, Pekka Collin, Kaija-leena Kolho, Erkki Savilahti, Markku MakiAbstract:Abstract Background & Aims: Tissue transglutaminase has been reported to be the target for endomysial antibodies in celiac disease. We sought to establish whether immunoglobulin (Ig) A class tissue transglutaminase autoantibodies can be considered specific for celiac disease. Methods: Serum samples from 136 patients with untreated celiac disease (diagnosed according to the criteria of the European Society for Pediatric Gastroenterology, Hepatology and Nutrition) and 207 disease controls were studied. Enzyme-linked immunosorbent assay (ELISA) and Western blots were performed using calcium-treated and untreated tissue transglutaminase as antigen. Reticulin, endomysial, and mouse monoclonal tissue transglutaminase antibodies were studied by an indirect immunofluorescence method and Gliadin antibodies with ELISA. Results: The calcium-activated tissue transglutaminase autoAntibody ELISA was highly sensitive (129 of 136) and specific (194 of 207) in detecting celiac disease. The new autoAntibody ELISA test correlated well with the endomysial Antibody test. Tissue transglutaminase autoAntibody ELISA showed a clearly better predictive potential than the IgA class Gliadin Antibody ELISA. Immunoblots and ELISA blocking studies showed that calcium is needed for the specific antigen-Antibody reaction to occur. Double immunofluorescence staining in human umbilical cord with sera from patients with celiac disease and with monoclonal tissue transglutaminase antibodies showed complete overlap. Conclusions: Calcium-activated tissue transglutaminase autoAntibody ELISA is highly accurate in detecting untreated celiac disease. Tissue transglutaminase seems to be the target self-antigen for endomysial antibodies. GASTROENTEROLOGY 1998;115:1322-1328
-
tissue transglutaminase autoAntibody enzyme linked immunosorbent assay in detecting celiac disease
Gastroenterology, 1998Co-Authors: S Sulkanen, Tuula Halttunen, Kaija Laurila, Ilma Rita Korponayszabo, Annikki Sarnesto, Pekka Collin, Kaija-leena Kolho, Erkki Savilahti, Markku MakiAbstract:BACKGROUND & AIMS: Tissue transglutaminase has been reported to be the target for endomysial antibodies in celiac disease. We sought to establish whether immunoglobulin (Ig) A class tissue transglutaminase autoantibodies can be considered specific for celiac disease. METHODS: Serum samples from 136 patients with untreated celiac disease (diagnosed according to the criteria of the European Society for Pediatric Gastroenterology, Hepatology and Nutrition) and 207 disease controls were studied. Enzyme-linked immunosorbent assay (ELISA) and Western blots were performed using calcium-treated and untreated tissue transglutaminase as antigen. Reticulin, endomysial, and mouse monoclonal tissue transglutaminase antibodies were studied by an indirect immunofluorescence method and Gliadin antibodies with ELISA. RESULTS: The calcium-activated tissue transglutaminase autoAntibody ELISA was highly sensitive (129 of 136) and specific (194 of 207) in detecting celiac disease. The new autoAntibody ELISA test correlated well with the endomysial Antibody test. Tissue transglutaminase autoAntibody ELISA showed a clearly better predictive potential than the IgA class Gliadin Antibody ELISA. Immunoblots and ELISA blocking studies showed that calcium is needed for the specific antigen-Antibody reaction to occur. Double immunofluorescence staining in human umbilical cord with sera from patients with celiac disease and with monoclonal tissue transglutaminase antibodies showed complete overlap. CONCLUSIONS: Calcium-activated tissue transglutaminase autoAntibody ELISA is highly accurate in detecting untreated celiac disease. Tissue transglutaminase seems to be the target self-antigen for endomysial antibodies.
-
small bowel mucosal inflammation in reticulin or Gliadin Antibody positive patients without villous atrophy
Scandinavian Journal of Gastroenterology, 1998Co-Authors: Katri Kaukinen, Annaliisa Karvonen, Pekka Collin, K Holm, P Pikkarainen, Markku MakiAbstract:Background: We investigated whether individuals with positive coeliac disease antibodies but without small-bowel villous atrophy have mucosal inflammation implicating gluten-sensitivity. Methods: Small-bowel mucosal morphology: CD3 + , αβ + , and γδ + T-cell receptor-bearing intraepithelial lymphocytes: and mucosal HLA-DR expression were studied in 96 IgA-class antireticulin or antiGliadin Antibody-positive adults suspected of having coeliac disease and in 27 control subjects. Results: Villous atrophy compatible with coeliac disease was found in altogether 29 patients, in 18 of 21 (86%) patients with both antireticulin and antiGliadin antibodies, in 9 of 15 (60%) patients with antireticulin antibodies only, and in 2 of 60 (3%) with antiGliadin antibodies only. In 67 Antibody-positive patients with normal villous architecture the densities of CD3 - . αβ + , and γδ + intraepithelial lymphocytes were significantly higher than in non-coeliac control subjects. Ten patients with initially increased densities of γδ + T cells but normal villous structure underwent a follow-up biopsy after 4-18 months, which showed villous atrophy in five patients. Conclusions: IgA-class antireticulin or antiGliadin Antibody-positive patients with normal small-bowel mucosal morphology frequently have immunohistochemical markers of coeliac disease latency. Together with our follow-up data this implies that they may be gluten-sensitive.
-
small bowel mucosal inflammation in reticulin or Gliadin Antibody positive patients without villous atrophy
Scandinavian Journal of Gastroenterology, 1998Co-Authors: Katri Kaukinen, Annaliisa Karvonen, Pekka Collin, K Holm, P Pikkarainen, Markku MakiAbstract:BACKGROUND We investigated whether individuals with positive coeliac disease antibodies but without small-bowel villous atrophy have mucosal inflammation implicating gluten-sensitivity. METHODS Small-bowel mucosal morphology; CD3+, alphabeta+, and gammadelta+ T-cell receptor-bearing intraepithelial lymphocytes; and mucosal HLA-DR expression were studied in 96 IgA-class antireticulin or antiGliadin Antibody-positive adults suspected of having coeliac disease and in 27 control subjects. RESULTS Villous atrophy compatible with coeliac disease was found in altogether 29 patients, in 18 of 21 (86%) patients with both antireticulin and antiGliadin antibodies, in 9 of 15 (60%) patients with antireticulin antibodies only, and in 2 of 60 (3%) with antiGliadin antibodies only. In 67 Antibody-positive patients with normal villous architecture the densities of CD3+, alphabeta+, and gammadelta+ intraepithelial lymphocytes were significantly higher than in non-coeliac control subjects. Ten patients with initially increased densities of gammadelta+ T cells but normal villous structure underwent a follow-up biopsy after 4-18 months, which showed villous atrophy in five patients. CONCLUSIONS IgA-class antireticulin or antiGliadin Antibody-positive patients with normal small-bowel mucosal morphology frequently have immunohistochemical markers of coeliac disease latency. Together with our follow-up data this implies that they may be gluten-sensitive.
Pekka Collin - One of the best experts on this subject based on the ideXlab platform.
-
tissue transglutaminase autoAntibody enzyme linked immunosorbent assay in detecting celiac disease
Gastroenterology, 1998Co-Authors: S Sulkanen, Tuula Halttunen, Kaija Laurila, Ilma Rita Korponayszabo, Annikki Sarnesto, Pekka Collin, Kaija-leena Kolho, Erkki Savilahti, Markku MakiAbstract:BACKGROUND & AIMS: Tissue transglutaminase has been reported to be the target for endomysial antibodies in celiac disease. We sought to establish whether immunoglobulin (Ig) A class tissue transglutaminase autoantibodies can be considered specific for celiac disease. METHODS: Serum samples from 136 patients with untreated celiac disease (diagnosed according to the criteria of the European Society for Pediatric Gastroenterology, Hepatology and Nutrition) and 207 disease controls were studied. Enzyme-linked immunosorbent assay (ELISA) and Western blots were performed using calcium-treated and untreated tissue transglutaminase as antigen. Reticulin, endomysial, and mouse monoclonal tissue transglutaminase antibodies were studied by an indirect immunofluorescence method and Gliadin antibodies with ELISA. RESULTS: The calcium-activated tissue transglutaminase autoAntibody ELISA was highly sensitive (129 of 136) and specific (194 of 207) in detecting celiac disease. The new autoAntibody ELISA test correlated well with the endomysial Antibody test. Tissue transglutaminase autoAntibody ELISA showed a clearly better predictive potential than the IgA class Gliadin Antibody ELISA. Immunoblots and ELISA blocking studies showed that calcium is needed for the specific antigen-Antibody reaction to occur. Double immunofluorescence staining in human umbilical cord with sera from patients with celiac disease and with monoclonal tissue transglutaminase antibodies showed complete overlap. CONCLUSIONS: Calcium-activated tissue transglutaminase autoAntibody ELISA is highly accurate in detecting untreated celiac disease. Tissue transglutaminase seems to be the target self-antigen for endomysial antibodies.
-
tissue transglutaminase autoAntibody enzyme linked immunosorbent assay in detecting celiac disease
Gastroenterology, 1998Co-Authors: S Sulkanen, Tuula Halttunen, Kaija Laurila, Ilma Rita Korponayszabo, Annikki Sarnesto, Pekka Collin, Kaija-leena Kolho, Erkki Savilahti, Markku MakiAbstract:Abstract Background & Aims: Tissue transglutaminase has been reported to be the target for endomysial antibodies in celiac disease. We sought to establish whether immunoglobulin (Ig) A class tissue transglutaminase autoantibodies can be considered specific for celiac disease. Methods: Serum samples from 136 patients with untreated celiac disease (diagnosed according to the criteria of the European Society for Pediatric Gastroenterology, Hepatology and Nutrition) and 207 disease controls were studied. Enzyme-linked immunosorbent assay (ELISA) and Western blots were performed using calcium-treated and untreated tissue transglutaminase as antigen. Reticulin, endomysial, and mouse monoclonal tissue transglutaminase antibodies were studied by an indirect immunofluorescence method and Gliadin antibodies with ELISA. Results: The calcium-activated tissue transglutaminase autoAntibody ELISA was highly sensitive (129 of 136) and specific (194 of 207) in detecting celiac disease. The new autoAntibody ELISA test correlated well with the endomysial Antibody test. Tissue transglutaminase autoAntibody ELISA showed a clearly better predictive potential than the IgA class Gliadin Antibody ELISA. Immunoblots and ELISA blocking studies showed that calcium is needed for the specific antigen-Antibody reaction to occur. Double immunofluorescence staining in human umbilical cord with sera from patients with celiac disease and with monoclonal tissue transglutaminase antibodies showed complete overlap. Conclusions: Calcium-activated tissue transglutaminase autoAntibody ELISA is highly accurate in detecting untreated celiac disease. Tissue transglutaminase seems to be the target self-antigen for endomysial antibodies. GASTROENTEROLOGY 1998;115:1322-1328
-
tissue transglutaminase autoAntibody enzyme linked immunosorbent assay in detecting celiac disease
Gastroenterology, 1998Co-Authors: S Sulkanen, Tuula Halttunen, Kaija Laurila, Ilma Rita Korponayszabo, Annikki Sarnesto, Pekka Collin, Kaija-leena Kolho, Erkki Savilahti, Markku MakiAbstract:BACKGROUND & AIMS: Tissue transglutaminase has been reported to be the target for endomysial antibodies in celiac disease. We sought to establish whether immunoglobulin (Ig) A class tissue transglutaminase autoantibodies can be considered specific for celiac disease. METHODS: Serum samples from 136 patients with untreated celiac disease (diagnosed according to the criteria of the European Society for Pediatric Gastroenterology, Hepatology and Nutrition) and 207 disease controls were studied. Enzyme-linked immunosorbent assay (ELISA) and Western blots were performed using calcium-treated and untreated tissue transglutaminase as antigen. Reticulin, endomysial, and mouse monoclonal tissue transglutaminase antibodies were studied by an indirect immunofluorescence method and Gliadin antibodies with ELISA. RESULTS: The calcium-activated tissue transglutaminase autoAntibody ELISA was highly sensitive (129 of 136) and specific (194 of 207) in detecting celiac disease. The new autoAntibody ELISA test correlated well with the endomysial Antibody test. Tissue transglutaminase autoAntibody ELISA showed a clearly better predictive potential than the IgA class Gliadin Antibody ELISA. Immunoblots and ELISA blocking studies showed that calcium is needed for the specific antigen-Antibody reaction to occur. Double immunofluorescence staining in human umbilical cord with sera from patients with celiac disease and with monoclonal tissue transglutaminase antibodies showed complete overlap. CONCLUSIONS: Calcium-activated tissue transglutaminase autoAntibody ELISA is highly accurate in detecting untreated celiac disease. Tissue transglutaminase seems to be the target self-antigen for endomysial antibodies.
-
small bowel mucosal inflammation in reticulin or Gliadin Antibody positive patients without villous atrophy
Scandinavian Journal of Gastroenterology, 1998Co-Authors: Katri Kaukinen, Annaliisa Karvonen, Pekka Collin, K Holm, P Pikkarainen, Markku MakiAbstract:Background: We investigated whether individuals with positive coeliac disease antibodies but without small-bowel villous atrophy have mucosal inflammation implicating gluten-sensitivity. Methods: Small-bowel mucosal morphology: CD3 + , αβ + , and γδ + T-cell receptor-bearing intraepithelial lymphocytes: and mucosal HLA-DR expression were studied in 96 IgA-class antireticulin or antiGliadin Antibody-positive adults suspected of having coeliac disease and in 27 control subjects. Results: Villous atrophy compatible with coeliac disease was found in altogether 29 patients, in 18 of 21 (86%) patients with both antireticulin and antiGliadin antibodies, in 9 of 15 (60%) patients with antireticulin antibodies only, and in 2 of 60 (3%) with antiGliadin antibodies only. In 67 Antibody-positive patients with normal villous architecture the densities of CD3 - . αβ + , and γδ + intraepithelial lymphocytes were significantly higher than in non-coeliac control subjects. Ten patients with initially increased densities of γδ + T cells but normal villous structure underwent a follow-up biopsy after 4-18 months, which showed villous atrophy in five patients. Conclusions: IgA-class antireticulin or antiGliadin Antibody-positive patients with normal small-bowel mucosal morphology frequently have immunohistochemical markers of coeliac disease latency. Together with our follow-up data this implies that they may be gluten-sensitive.
-
small bowel mucosal inflammation in reticulin or Gliadin Antibody positive patients without villous atrophy
Scandinavian Journal of Gastroenterology, 1998Co-Authors: Katri Kaukinen, Annaliisa Karvonen, Pekka Collin, K Holm, P Pikkarainen, Markku MakiAbstract:BACKGROUND We investigated whether individuals with positive coeliac disease antibodies but without small-bowel villous atrophy have mucosal inflammation implicating gluten-sensitivity. METHODS Small-bowel mucosal morphology; CD3+, alphabeta+, and gammadelta+ T-cell receptor-bearing intraepithelial lymphocytes; and mucosal HLA-DR expression were studied in 96 IgA-class antireticulin or antiGliadin Antibody-positive adults suspected of having coeliac disease and in 27 control subjects. RESULTS Villous atrophy compatible with coeliac disease was found in altogether 29 patients, in 18 of 21 (86%) patients with both antireticulin and antiGliadin antibodies, in 9 of 15 (60%) patients with antireticulin antibodies only, and in 2 of 60 (3%) with antiGliadin antibodies only. In 67 Antibody-positive patients with normal villous architecture the densities of CD3+, alphabeta+, and gammadelta+ intraepithelial lymphocytes were significantly higher than in non-coeliac control subjects. Ten patients with initially increased densities of gammadelta+ T cells but normal villous structure underwent a follow-up biopsy after 4-18 months, which showed villous atrophy in five patients. CONCLUSIONS IgA-class antireticulin or antiGliadin Antibody-positive patients with normal small-bowel mucosal morphology frequently have immunohistochemical markers of coeliac disease latency. Together with our follow-up data this implies that they may be gluten-sensitive.
Ilma Rita Korponayszabo - One of the best experts on this subject based on the ideXlab platform.
-
tissue transglutaminase autoAntibody enzyme linked immunosorbent assay in detecting celiac disease
Gastroenterology, 1998Co-Authors: S Sulkanen, Tuula Halttunen, Kaija Laurila, Ilma Rita Korponayszabo, Annikki Sarnesto, Pekka Collin, Kaija-leena Kolho, Erkki Savilahti, Markku MakiAbstract:BACKGROUND & AIMS: Tissue transglutaminase has been reported to be the target for endomysial antibodies in celiac disease. We sought to establish whether immunoglobulin (Ig) A class tissue transglutaminase autoantibodies can be considered specific for celiac disease. METHODS: Serum samples from 136 patients with untreated celiac disease (diagnosed according to the criteria of the European Society for Pediatric Gastroenterology, Hepatology and Nutrition) and 207 disease controls were studied. Enzyme-linked immunosorbent assay (ELISA) and Western blots were performed using calcium-treated and untreated tissue transglutaminase as antigen. Reticulin, endomysial, and mouse monoclonal tissue transglutaminase antibodies were studied by an indirect immunofluorescence method and Gliadin antibodies with ELISA. RESULTS: The calcium-activated tissue transglutaminase autoAntibody ELISA was highly sensitive (129 of 136) and specific (194 of 207) in detecting celiac disease. The new autoAntibody ELISA test correlated well with the endomysial Antibody test. Tissue transglutaminase autoAntibody ELISA showed a clearly better predictive potential than the IgA class Gliadin Antibody ELISA. Immunoblots and ELISA blocking studies showed that calcium is needed for the specific antigen-Antibody reaction to occur. Double immunofluorescence staining in human umbilical cord with sera from patients with celiac disease and with monoclonal tissue transglutaminase antibodies showed complete overlap. CONCLUSIONS: Calcium-activated tissue transglutaminase autoAntibody ELISA is highly accurate in detecting untreated celiac disease. Tissue transglutaminase seems to be the target self-antigen for endomysial antibodies.
-
tissue transglutaminase autoAntibody enzyme linked immunosorbent assay in detecting celiac disease
Gastroenterology, 1998Co-Authors: S Sulkanen, Tuula Halttunen, Kaija Laurila, Ilma Rita Korponayszabo, Annikki Sarnesto, Pekka Collin, Kaija-leena Kolho, Erkki Savilahti, Markku MakiAbstract:Abstract Background & Aims: Tissue transglutaminase has been reported to be the target for endomysial antibodies in celiac disease. We sought to establish whether immunoglobulin (Ig) A class tissue transglutaminase autoantibodies can be considered specific for celiac disease. Methods: Serum samples from 136 patients with untreated celiac disease (diagnosed according to the criteria of the European Society for Pediatric Gastroenterology, Hepatology and Nutrition) and 207 disease controls were studied. Enzyme-linked immunosorbent assay (ELISA) and Western blots were performed using calcium-treated and untreated tissue transglutaminase as antigen. Reticulin, endomysial, and mouse monoclonal tissue transglutaminase antibodies were studied by an indirect immunofluorescence method and Gliadin antibodies with ELISA. Results: The calcium-activated tissue transglutaminase autoAntibody ELISA was highly sensitive (129 of 136) and specific (194 of 207) in detecting celiac disease. The new autoAntibody ELISA test correlated well with the endomysial Antibody test. Tissue transglutaminase autoAntibody ELISA showed a clearly better predictive potential than the IgA class Gliadin Antibody ELISA. Immunoblots and ELISA blocking studies showed that calcium is needed for the specific antigen-Antibody reaction to occur. Double immunofluorescence staining in human umbilical cord with sera from patients with celiac disease and with monoclonal tissue transglutaminase antibodies showed complete overlap. Conclusions: Calcium-activated tissue transglutaminase autoAntibody ELISA is highly accurate in detecting untreated celiac disease. Tissue transglutaminase seems to be the target self-antigen for endomysial antibodies. GASTROENTEROLOGY 1998;115:1322-1328
-
tissue transglutaminase autoAntibody enzyme linked immunosorbent assay in detecting celiac disease
Gastroenterology, 1998Co-Authors: S Sulkanen, Tuula Halttunen, Kaija Laurila, Ilma Rita Korponayszabo, Annikki Sarnesto, Pekka Collin, Kaija-leena Kolho, Erkki Savilahti, Markku MakiAbstract:BACKGROUND & AIMS: Tissue transglutaminase has been reported to be the target for endomysial antibodies in celiac disease. We sought to establish whether immunoglobulin (Ig) A class tissue transglutaminase autoantibodies can be considered specific for celiac disease. METHODS: Serum samples from 136 patients with untreated celiac disease (diagnosed according to the criteria of the European Society for Pediatric Gastroenterology, Hepatology and Nutrition) and 207 disease controls were studied. Enzyme-linked immunosorbent assay (ELISA) and Western blots were performed using calcium-treated and untreated tissue transglutaminase as antigen. Reticulin, endomysial, and mouse monoclonal tissue transglutaminase antibodies were studied by an indirect immunofluorescence method and Gliadin antibodies with ELISA. RESULTS: The calcium-activated tissue transglutaminase autoAntibody ELISA was highly sensitive (129 of 136) and specific (194 of 207) in detecting celiac disease. The new autoAntibody ELISA test correlated well with the endomysial Antibody test. Tissue transglutaminase autoAntibody ELISA showed a clearly better predictive potential than the IgA class Gliadin Antibody ELISA. Immunoblots and ELISA blocking studies showed that calcium is needed for the specific antigen-Antibody reaction to occur. Double immunofluorescence staining in human umbilical cord with sera from patients with celiac disease and with monoclonal tissue transglutaminase antibodies showed complete overlap. CONCLUSIONS: Calcium-activated tissue transglutaminase autoAntibody ELISA is highly accurate in detecting untreated celiac disease. Tissue transglutaminase seems to be the target self-antigen for endomysial antibodies.
Erkki Savilahti - One of the best experts on this subject based on the ideXlab platform.
-
tissue transglutaminase autoAntibody enzyme linked immunosorbent assay in detecting celiac disease
Gastroenterology, 1998Co-Authors: S Sulkanen, Tuula Halttunen, Kaija Laurila, Ilma Rita Korponayszabo, Annikki Sarnesto, Pekka Collin, Kaija-leena Kolho, Erkki Savilahti, Markku MakiAbstract:BACKGROUND & AIMS: Tissue transglutaminase has been reported to be the target for endomysial antibodies in celiac disease. We sought to establish whether immunoglobulin (Ig) A class tissue transglutaminase autoantibodies can be considered specific for celiac disease. METHODS: Serum samples from 136 patients with untreated celiac disease (diagnosed according to the criteria of the European Society for Pediatric Gastroenterology, Hepatology and Nutrition) and 207 disease controls were studied. Enzyme-linked immunosorbent assay (ELISA) and Western blots were performed using calcium-treated and untreated tissue transglutaminase as antigen. Reticulin, endomysial, and mouse monoclonal tissue transglutaminase antibodies were studied by an indirect immunofluorescence method and Gliadin antibodies with ELISA. RESULTS: The calcium-activated tissue transglutaminase autoAntibody ELISA was highly sensitive (129 of 136) and specific (194 of 207) in detecting celiac disease. The new autoAntibody ELISA test correlated well with the endomysial Antibody test. Tissue transglutaminase autoAntibody ELISA showed a clearly better predictive potential than the IgA class Gliadin Antibody ELISA. Immunoblots and ELISA blocking studies showed that calcium is needed for the specific antigen-Antibody reaction to occur. Double immunofluorescence staining in human umbilical cord with sera from patients with celiac disease and with monoclonal tissue transglutaminase antibodies showed complete overlap. CONCLUSIONS: Calcium-activated tissue transglutaminase autoAntibody ELISA is highly accurate in detecting untreated celiac disease. Tissue transglutaminase seems to be the target self-antigen for endomysial antibodies.
-
tissue transglutaminase autoAntibody enzyme linked immunosorbent assay in detecting celiac disease
Gastroenterology, 1998Co-Authors: S Sulkanen, Tuula Halttunen, Kaija Laurila, Ilma Rita Korponayszabo, Annikki Sarnesto, Pekka Collin, Kaija-leena Kolho, Erkki Savilahti, Markku MakiAbstract:Abstract Background & Aims: Tissue transglutaminase has been reported to be the target for endomysial antibodies in celiac disease. We sought to establish whether immunoglobulin (Ig) A class tissue transglutaminase autoantibodies can be considered specific for celiac disease. Methods: Serum samples from 136 patients with untreated celiac disease (diagnosed according to the criteria of the European Society for Pediatric Gastroenterology, Hepatology and Nutrition) and 207 disease controls were studied. Enzyme-linked immunosorbent assay (ELISA) and Western blots were performed using calcium-treated and untreated tissue transglutaminase as antigen. Reticulin, endomysial, and mouse monoclonal tissue transglutaminase antibodies were studied by an indirect immunofluorescence method and Gliadin antibodies with ELISA. Results: The calcium-activated tissue transglutaminase autoAntibody ELISA was highly sensitive (129 of 136) and specific (194 of 207) in detecting celiac disease. The new autoAntibody ELISA test correlated well with the endomysial Antibody test. Tissue transglutaminase autoAntibody ELISA showed a clearly better predictive potential than the IgA class Gliadin Antibody ELISA. Immunoblots and ELISA blocking studies showed that calcium is needed for the specific antigen-Antibody reaction to occur. Double immunofluorescence staining in human umbilical cord with sera from patients with celiac disease and with monoclonal tissue transglutaminase antibodies showed complete overlap. Conclusions: Calcium-activated tissue transglutaminase autoAntibody ELISA is highly accurate in detecting untreated celiac disease. Tissue transglutaminase seems to be the target self-antigen for endomysial antibodies. GASTROENTEROLOGY 1998;115:1322-1328
-
tissue transglutaminase autoAntibody enzyme linked immunosorbent assay in detecting celiac disease
Gastroenterology, 1998Co-Authors: S Sulkanen, Tuula Halttunen, Kaija Laurila, Ilma Rita Korponayszabo, Annikki Sarnesto, Pekka Collin, Kaija-leena Kolho, Erkki Savilahti, Markku MakiAbstract:BACKGROUND & AIMS: Tissue transglutaminase has been reported to be the target for endomysial antibodies in celiac disease. We sought to establish whether immunoglobulin (Ig) A class tissue transglutaminase autoantibodies can be considered specific for celiac disease. METHODS: Serum samples from 136 patients with untreated celiac disease (diagnosed according to the criteria of the European Society for Pediatric Gastroenterology, Hepatology and Nutrition) and 207 disease controls were studied. Enzyme-linked immunosorbent assay (ELISA) and Western blots were performed using calcium-treated and untreated tissue transglutaminase as antigen. Reticulin, endomysial, and mouse monoclonal tissue transglutaminase antibodies were studied by an indirect immunofluorescence method and Gliadin antibodies with ELISA. RESULTS: The calcium-activated tissue transglutaminase autoAntibody ELISA was highly sensitive (129 of 136) and specific (194 of 207) in detecting celiac disease. The new autoAntibody ELISA test correlated well with the endomysial Antibody test. Tissue transglutaminase autoAntibody ELISA showed a clearly better predictive potential than the IgA class Gliadin Antibody ELISA. Immunoblots and ELISA blocking studies showed that calcium is needed for the specific antigen-Antibody reaction to occur. Double immunofluorescence staining in human umbilical cord with sera from patients with celiac disease and with monoclonal tissue transglutaminase antibodies showed complete overlap. CONCLUSIONS: Calcium-activated tissue transglutaminase autoAntibody ELISA is highly accurate in detecting untreated celiac disease. Tissue transglutaminase seems to be the target self-antigen for endomysial antibodies.
-
iga endomysium antibodies on human umbilical cord an excellent diagnostic tool for celiac disease in childhood
Journal of Pediatric Gastroenterology and Nutrition, 1997Co-Authors: Kaija-leena Kolho, Erkki SavilahtiAbstract:Background: An improvement in screening for celiac disease has recently been described that uses human umbilical cord as a substitute for monkey esophagus to determine IgA endomysium antibodies in adults. As using monkey esophagus is ethically questionable for large-scale screening, we studied whether substitution of umbilical cord would be suitable for pediatric patients as well. Methods: Serum from 53 children with untreated celiac disease, 22 in remission and 13 on challenge, were screened for antiGliadin IgA, antiGliadin IgG, and IgA reticulin antibodies, in addition to IgA endomysium antibodies tested both on monkey esophagus and on human umbilical cord. Controls included 20 patients with cow-milk-sensitive enteropathy, 23 with inflammatory bowel disease, and 23 with diabetes mellitus, and 48 patients who were biopsied to exclude celiac disease either because of positive Gliadin Antibody test or disturbed growth. Results: Sensitivity (0.94) and specificity (1.0) were similar for umbilical cord and esophageal determinations in active celiac disease. Both substrates detected identical positive cases and neither gave false-positive results. In celiac patients on a gluten-free diet, endomysium antibodies with either substrate were positive in seven identical cases and negative in 15 of 22 cases. Correlations with reticulin antibodies were comparable with human umbilical cord and monkey esophagus (0.83 and 0.85, respectively; Spearman Correlation Section Pair-Wise deletion). Conclusions: Human umbilical cord is an excellent substitute for monkey esophagus to determine endomysium antibodies in celiac diagnosis in children and adolescents.
S Sulkanen - One of the best experts on this subject based on the ideXlab platform.
-
tissue transglutaminase autoAntibody enzyme linked immunosorbent assay in detecting celiac disease
Gastroenterology, 1998Co-Authors: S Sulkanen, Tuula Halttunen, Kaija Laurila, Ilma Rita Korponayszabo, Annikki Sarnesto, Pekka Collin, Kaija-leena Kolho, Erkki Savilahti, Markku MakiAbstract:BACKGROUND & AIMS: Tissue transglutaminase has been reported to be the target for endomysial antibodies in celiac disease. We sought to establish whether immunoglobulin (Ig) A class tissue transglutaminase autoantibodies can be considered specific for celiac disease. METHODS: Serum samples from 136 patients with untreated celiac disease (diagnosed according to the criteria of the European Society for Pediatric Gastroenterology, Hepatology and Nutrition) and 207 disease controls were studied. Enzyme-linked immunosorbent assay (ELISA) and Western blots were performed using calcium-treated and untreated tissue transglutaminase as antigen. Reticulin, endomysial, and mouse monoclonal tissue transglutaminase antibodies were studied by an indirect immunofluorescence method and Gliadin antibodies with ELISA. RESULTS: The calcium-activated tissue transglutaminase autoAntibody ELISA was highly sensitive (129 of 136) and specific (194 of 207) in detecting celiac disease. The new autoAntibody ELISA test correlated well with the endomysial Antibody test. Tissue transglutaminase autoAntibody ELISA showed a clearly better predictive potential than the IgA class Gliadin Antibody ELISA. Immunoblots and ELISA blocking studies showed that calcium is needed for the specific antigen-Antibody reaction to occur. Double immunofluorescence staining in human umbilical cord with sera from patients with celiac disease and with monoclonal tissue transglutaminase antibodies showed complete overlap. CONCLUSIONS: Calcium-activated tissue transglutaminase autoAntibody ELISA is highly accurate in detecting untreated celiac disease. Tissue transglutaminase seems to be the target self-antigen for endomysial antibodies.
-
tissue transglutaminase autoAntibody enzyme linked immunosorbent assay in detecting celiac disease
Gastroenterology, 1998Co-Authors: S Sulkanen, Tuula Halttunen, Kaija Laurila, Ilma Rita Korponayszabo, Annikki Sarnesto, Pekka Collin, Kaija-leena Kolho, Erkki Savilahti, Markku MakiAbstract:Abstract Background & Aims: Tissue transglutaminase has been reported to be the target for endomysial antibodies in celiac disease. We sought to establish whether immunoglobulin (Ig) A class tissue transglutaminase autoantibodies can be considered specific for celiac disease. Methods: Serum samples from 136 patients with untreated celiac disease (diagnosed according to the criteria of the European Society for Pediatric Gastroenterology, Hepatology and Nutrition) and 207 disease controls were studied. Enzyme-linked immunosorbent assay (ELISA) and Western blots were performed using calcium-treated and untreated tissue transglutaminase as antigen. Reticulin, endomysial, and mouse monoclonal tissue transglutaminase antibodies were studied by an indirect immunofluorescence method and Gliadin antibodies with ELISA. Results: The calcium-activated tissue transglutaminase autoAntibody ELISA was highly sensitive (129 of 136) and specific (194 of 207) in detecting celiac disease. The new autoAntibody ELISA test correlated well with the endomysial Antibody test. Tissue transglutaminase autoAntibody ELISA showed a clearly better predictive potential than the IgA class Gliadin Antibody ELISA. Immunoblots and ELISA blocking studies showed that calcium is needed for the specific antigen-Antibody reaction to occur. Double immunofluorescence staining in human umbilical cord with sera from patients with celiac disease and with monoclonal tissue transglutaminase antibodies showed complete overlap. Conclusions: Calcium-activated tissue transglutaminase autoAntibody ELISA is highly accurate in detecting untreated celiac disease. Tissue transglutaminase seems to be the target self-antigen for endomysial antibodies. GASTROENTEROLOGY 1998;115:1322-1328
-
tissue transglutaminase autoAntibody enzyme linked immunosorbent assay in detecting celiac disease
Gastroenterology, 1998Co-Authors: S Sulkanen, Tuula Halttunen, Kaija Laurila, Ilma Rita Korponayszabo, Annikki Sarnesto, Pekka Collin, Kaija-leena Kolho, Erkki Savilahti, Markku MakiAbstract:BACKGROUND & AIMS: Tissue transglutaminase has been reported to be the target for endomysial antibodies in celiac disease. We sought to establish whether immunoglobulin (Ig) A class tissue transglutaminase autoantibodies can be considered specific for celiac disease. METHODS: Serum samples from 136 patients with untreated celiac disease (diagnosed according to the criteria of the European Society for Pediatric Gastroenterology, Hepatology and Nutrition) and 207 disease controls were studied. Enzyme-linked immunosorbent assay (ELISA) and Western blots were performed using calcium-treated and untreated tissue transglutaminase as antigen. Reticulin, endomysial, and mouse monoclonal tissue transglutaminase antibodies were studied by an indirect immunofluorescence method and Gliadin antibodies with ELISA. RESULTS: The calcium-activated tissue transglutaminase autoAntibody ELISA was highly sensitive (129 of 136) and specific (194 of 207) in detecting celiac disease. The new autoAntibody ELISA test correlated well with the endomysial Antibody test. Tissue transglutaminase autoAntibody ELISA showed a clearly better predictive potential than the IgA class Gliadin Antibody ELISA. Immunoblots and ELISA blocking studies showed that calcium is needed for the specific antigen-Antibody reaction to occur. Double immunofluorescence staining in human umbilical cord with sera from patients with celiac disease and with monoclonal tissue transglutaminase antibodies showed complete overlap. CONCLUSIONS: Calcium-activated tissue transglutaminase autoAntibody ELISA is highly accurate in detecting untreated celiac disease. Tissue transglutaminase seems to be the target self-antigen for endomysial antibodies.