The Experts below are selected from a list of 60 Experts worldwide ranked by ideXlab platform

Justin Sardi - One of the best experts on this subject based on the ideXlab platform.

  • analysis of the three dimensional structure of the kidney Glomerulus Capillary network
    Scientific Reports, 2020
    Co-Authors: Mark Terasaki, Jason Cory Brunson, Justin Sardi
    Abstract:

    The Capillary network of the kidney Glomerulus filters small molecules from the blood. The glomerular 3D structure should help to understand its function, but it is poorly characterized. We therefore devised a new approach in which an automated tape collecting microtome (ATUM) was used to collect 0.5 μm thick serial sections from fixed mouse kidneys. The sections were imaged by scanning electron microscopy at ~ 50 nm/pixel resolution. With this approach, 12 glomeruli were reconstructed at an x-y-z resolution ~ 10 × higher than that of paraffin sections. We found a previously undescribed no-cross zone between afferent and efferent branches on the vascular pole side; connections here would allow blood to exit without being adequately filtered. The Capillary diameters throughout the Glomerulus appeared to correspond with the amount of blood flow within them. The shortest path (minimum number of branches to travel from afferent to efferent arterioles) is relatively independent of glomerular size and is present primarily on the vascular pole size. This suggests that new branches and longer paths form on the urinary pole side. Network analysis indicates that the glomerular network does not form by repetitive longitudinal splitting of capillaries. Thus the 3D structure of the glomerular Capillary network provides useful information with which to understand glomerular function. Other tissue structures in the body may benefit from this new three dimensional approach.

  • Analysis of the three dimensional structure of the kidney Glomerulus Capillary network
    bioRxiv, 2019
    Co-Authors: Mark Terasaki, Jason Corey Brunson, Justin Sardi
    Abstract:

    The Capillary network of the kidney Glomerulus filters small molecules from the blood. The glomerular 3D structure should help to understand its function, but it is poorly characterized. We therefore devised a new approach in which an automated tape collecting microtome (ATUM) was used to collect 0.5 micron thick serial sections from fixed mouse kidneys. The sections were imaged by scanning electron microscopy at ~50 nm/pixel resolution. With this approach, 12 glomeruli were reconstructed at an x-y-z resolution ~10x higher than that of paraffin sections. We found a no-cross zone between afferent and efferent branches on the vascular pole side; connections here could allow blood to exit without being adequately filtered. Network analysis indicates that the glomerular network does not form by repetitive longitudinal splitting of capillaries. It also suggests that capillaries vary their diameter to make flow more efficient. The shortest path (minimum number of branches to travel from afferent to efferent arterioles) is relatively independent of glomerular size and is present primarily on the vascular pole size. This suggests that the shortest path is established on the vascular pole side, after which new branches and longer paths form on the urinary pole side. Thus the 3D structure of the glomerular Capillary network provides useful information with which to understand glomerular function. Other tissue structures in the body may benefit from this new three dimensional approach.

Mark Terasaki - One of the best experts on this subject based on the ideXlab platform.

  • analysis of the three dimensional structure of the kidney Glomerulus Capillary network
    Scientific Reports, 2020
    Co-Authors: Mark Terasaki, Jason Cory Brunson, Justin Sardi
    Abstract:

    The Capillary network of the kidney Glomerulus filters small molecules from the blood. The glomerular 3D structure should help to understand its function, but it is poorly characterized. We therefore devised a new approach in which an automated tape collecting microtome (ATUM) was used to collect 0.5 μm thick serial sections from fixed mouse kidneys. The sections were imaged by scanning electron microscopy at ~ 50 nm/pixel resolution. With this approach, 12 glomeruli were reconstructed at an x-y-z resolution ~ 10 × higher than that of paraffin sections. We found a previously undescribed no-cross zone between afferent and efferent branches on the vascular pole side; connections here would allow blood to exit without being adequately filtered. The Capillary diameters throughout the Glomerulus appeared to correspond with the amount of blood flow within them. The shortest path (minimum number of branches to travel from afferent to efferent arterioles) is relatively independent of glomerular size and is present primarily on the vascular pole size. This suggests that new branches and longer paths form on the urinary pole side. Network analysis indicates that the glomerular network does not form by repetitive longitudinal splitting of capillaries. Thus the 3D structure of the glomerular Capillary network provides useful information with which to understand glomerular function. Other tissue structures in the body may benefit from this new three dimensional approach.

  • Analysis of the three dimensional structure of the kidney Glomerulus Capillary network
    bioRxiv, 2019
    Co-Authors: Mark Terasaki, Jason Corey Brunson, Justin Sardi
    Abstract:

    The Capillary network of the kidney Glomerulus filters small molecules from the blood. The glomerular 3D structure should help to understand its function, but it is poorly characterized. We therefore devised a new approach in which an automated tape collecting microtome (ATUM) was used to collect 0.5 micron thick serial sections from fixed mouse kidneys. The sections were imaged by scanning electron microscopy at ~50 nm/pixel resolution. With this approach, 12 glomeruli were reconstructed at an x-y-z resolution ~10x higher than that of paraffin sections. We found a no-cross zone between afferent and efferent branches on the vascular pole side; connections here could allow blood to exit without being adequately filtered. Network analysis indicates that the glomerular network does not form by repetitive longitudinal splitting of capillaries. It also suggests that capillaries vary their diameter to make flow more efficient. The shortest path (minimum number of branches to travel from afferent to efferent arterioles) is relatively independent of glomerular size and is present primarily on the vascular pole size. This suggests that the shortest path is established on the vascular pole side, after which new branches and longer paths form on the urinary pole side. Thus the 3D structure of the glomerular Capillary network provides useful information with which to understand glomerular function. Other tissue structures in the body may benefit from this new three dimensional approach.

Miriam Frankenthal Figueira - One of the best experts on this subject based on the ideXlab platform.

  • Diabetic rats present higher urinary loss of proteins and lower renal expression of megalin, cubilin, ClC‐5, and CFTR
    Physiological Reports, 2017
    Co-Authors: Miriam Frankenthal Figueira, Raquel C Castiglione, Carolina M L Barbosa, Felipe M Ornellas, Rodrigo Nunes Da Fonseca, Georgia Da Silva Feltran, Marcelo M. Morales, Jackson Souza-menezes
    Abstract:

    Abstract Diabetic nephropathy (DN) occurs in around 40% of those with diabetes. Proteinuria is the main characteristic of DN and develops as a result of increased permeability of the Glomerulus Capillary wall and/or decreased proximal tubule endocytosis. The goal of this work was to evaluate renal function and the expression of megalin, cubilin, CFTR (cystic fibrosis transmembrane conductance regulator), and ClC‐5 in the proximal tubule and renal cortex of rats with type 1 diabetes. Male Wistar rats were randomly assigned to control (CTRL) and diabetic (DM) groups for 4 weeks. Renal function was assessed in 24‐h urine sample by calculating clearance and fractional excretion of solutes. The RNA and protein contents of ClC‐5, CFTR, megalin, and cubilin were determined in the renal proximal tubule and cortex using real‐time polymerase chain reaction and western blotting techniques, respectively. The results showed higher creatinine clearance and higher urinary excretion of proteins, albumin, and transferrin in the DM group than in the CTRL group. Furthermore, the renal cortex and proximal tubule of diabetic animals showed downregulation of megalin, cubilin, ClC‐5, and CFTR, critical components of the endocytic apparatus. These data suggest dysfunction in proximal tubule low‐molecular‐weight endocytosis and protein Glomerulus filtration in the kidney of diabetic rats.

  • diabetic rats present higher urinary loss of proteins and lower renal expression of megalin cubilin clc 5 and cftr
    Physiological Reports, 2017
    Co-Authors: Miriam Frankenthal Figueira, Raquel C Castiglione, Carolina M L Barbosa, Felipe M Ornellas, Rodrigo Nunes Da Fonseca, Georgia Da Silva Feltran, Marcelo M. Morales, Jackson Souzamenezes
    Abstract:

    Abstract Diabetic nephropathy (DN) occurs in around 40% of those with diabetes. Proteinuria is the main characteristic of DN and develops as a result of increased permeability of the Glomerulus Capillary wall and/or decreased proximal tubule endocytosis. The goal of this work was to evaluate renal function and the expression of megalin, cubilin, CFTR (cystic fibrosis transmembrane conductance regulator), and ClC‐5 in the proximal tubule and renal cortex of rats with type 1 diabetes. Male Wistar rats were randomly assigned to control (CTRL) and diabetic (DM) groups for 4 weeks. Renal function was assessed in 24‐h urine sample by calculating clearance and fractional excretion of solutes. The RNA and protein contents of ClC‐5, CFTR, megalin, and cubilin were determined in the renal proximal tubule and cortex using real‐time polymerase chain reaction and western blotting techniques, respectively. The results showed higher creatinine clearance and higher urinary excretion of proteins, albumin, and transferrin in the DM group than in the CTRL group. Furthermore, the renal cortex and proximal tubule of diabetic animals showed downregulation of megalin, cubilin, ClC‐5, and CFTR, critical components of the endocytic apparatus. These data suggest dysfunction in proximal tubule low‐molecular‐weight endocytosis and protein Glomerulus filtration in the kidney of diabetic rats.

  • Dataset for: Diabetic rats present higher urinary loss of proteins and lower renal expression of megalin, cubilin, ClC-5 and CFTR
    2017
    Co-Authors: Miriam Frankenthal Figueira, Raquel C Castiglione, Felipe M Ornellas, Rodrigo Nunes Da Fonseca, Georgia Da Silva Feltran, Marcelo M. Morales, Carolina Monteiro De Lemos Barbosa, Jackson Souza-menezes
    Abstract:

    Diabetic nephropathy (DN) occurs in around 40% of those with diabetes. Proteinuria is the main characteristic of DN and develops as a result of increased permeability of the Glomerulus Capillary wall and/or decreased proximal tubule endocytosis. The goal of this work was to evaluate renal function and the expression of megalin, cubilin, CFTR (cystic fibrosis transmembrane conductance regulator) and ClC-5 in the proximal tubule and renal cortex of rats with type 1 diabetes. Male Wistar rats were randomly assigned to control (CTRL) and diabetic (DM) groups for 4 weeks. Renal function was assessed in 24-h urine by calculating clearance and fractional excretion of solutes. The RNA and protein content of ClC-5, CFTR, megalin and cubilin was determined in the renal proximal tubule and cortex using real-time polymerase chain reaction and western blotting techniques, respectively. The results showed higher creatinine clearance and higher urinary excretion of proteins, albumin and transferrin in the DM group than in the CTRL group. Furthermore, the renal cortex and proximal tubule of diabetic animals showed downregulation of megalin, cubilin, ClC-5 and CFTR, critical components of the endocytic apparatus. These data suggest dysfunction in proximal tubule low-molecular-weight endocytosis and protein Glomerulus filtration in the kidney of diabetic rats

Jackson Souza-menezes - One of the best experts on this subject based on the ideXlab platform.

  • Diabetic rats present higher urinary loss of proteins and lower renal expression of megalin, cubilin, ClC‐5, and CFTR
    Physiological Reports, 2017
    Co-Authors: Miriam Frankenthal Figueira, Raquel C Castiglione, Carolina M L Barbosa, Felipe M Ornellas, Rodrigo Nunes Da Fonseca, Georgia Da Silva Feltran, Marcelo M. Morales, Jackson Souza-menezes
    Abstract:

    Abstract Diabetic nephropathy (DN) occurs in around 40% of those with diabetes. Proteinuria is the main characteristic of DN and develops as a result of increased permeability of the Glomerulus Capillary wall and/or decreased proximal tubule endocytosis. The goal of this work was to evaluate renal function and the expression of megalin, cubilin, CFTR (cystic fibrosis transmembrane conductance regulator), and ClC‐5 in the proximal tubule and renal cortex of rats with type 1 diabetes. Male Wistar rats were randomly assigned to control (CTRL) and diabetic (DM) groups for 4 weeks. Renal function was assessed in 24‐h urine sample by calculating clearance and fractional excretion of solutes. The RNA and protein contents of ClC‐5, CFTR, megalin, and cubilin were determined in the renal proximal tubule and cortex using real‐time polymerase chain reaction and western blotting techniques, respectively. The results showed higher creatinine clearance and higher urinary excretion of proteins, albumin, and transferrin in the DM group than in the CTRL group. Furthermore, the renal cortex and proximal tubule of diabetic animals showed downregulation of megalin, cubilin, ClC‐5, and CFTR, critical components of the endocytic apparatus. These data suggest dysfunction in proximal tubule low‐molecular‐weight endocytosis and protein Glomerulus filtration in the kidney of diabetic rats.

  • Dataset for: Diabetic rats present higher urinary loss of proteins and lower renal expression of megalin, cubilin, ClC-5 and CFTR
    2017
    Co-Authors: Miriam Frankenthal Figueira, Raquel C Castiglione, Felipe M Ornellas, Rodrigo Nunes Da Fonseca, Georgia Da Silva Feltran, Marcelo M. Morales, Carolina Monteiro De Lemos Barbosa, Jackson Souza-menezes
    Abstract:

    Diabetic nephropathy (DN) occurs in around 40% of those with diabetes. Proteinuria is the main characteristic of DN and develops as a result of increased permeability of the Glomerulus Capillary wall and/or decreased proximal tubule endocytosis. The goal of this work was to evaluate renal function and the expression of megalin, cubilin, CFTR (cystic fibrosis transmembrane conductance regulator) and ClC-5 in the proximal tubule and renal cortex of rats with type 1 diabetes. Male Wistar rats were randomly assigned to control (CTRL) and diabetic (DM) groups for 4 weeks. Renal function was assessed in 24-h urine by calculating clearance and fractional excretion of solutes. The RNA and protein content of ClC-5, CFTR, megalin and cubilin was determined in the renal proximal tubule and cortex using real-time polymerase chain reaction and western blotting techniques, respectively. The results showed higher creatinine clearance and higher urinary excretion of proteins, albumin and transferrin in the DM group than in the CTRL group. Furthermore, the renal cortex and proximal tubule of diabetic animals showed downregulation of megalin, cubilin, ClC-5 and CFTR, critical components of the endocytic apparatus. These data suggest dysfunction in proximal tubule low-molecular-weight endocytosis and protein Glomerulus filtration in the kidney of diabetic rats

Raquel C Castiglione - One of the best experts on this subject based on the ideXlab platform.

  • Diabetic rats present higher urinary loss of proteins and lower renal expression of megalin, cubilin, ClC‐5, and CFTR
    Physiological Reports, 2017
    Co-Authors: Miriam Frankenthal Figueira, Raquel C Castiglione, Carolina M L Barbosa, Felipe M Ornellas, Rodrigo Nunes Da Fonseca, Georgia Da Silva Feltran, Marcelo M. Morales, Jackson Souza-menezes
    Abstract:

    Abstract Diabetic nephropathy (DN) occurs in around 40% of those with diabetes. Proteinuria is the main characteristic of DN and develops as a result of increased permeability of the Glomerulus Capillary wall and/or decreased proximal tubule endocytosis. The goal of this work was to evaluate renal function and the expression of megalin, cubilin, CFTR (cystic fibrosis transmembrane conductance regulator), and ClC‐5 in the proximal tubule and renal cortex of rats with type 1 diabetes. Male Wistar rats were randomly assigned to control (CTRL) and diabetic (DM) groups for 4 weeks. Renal function was assessed in 24‐h urine sample by calculating clearance and fractional excretion of solutes. The RNA and protein contents of ClC‐5, CFTR, megalin, and cubilin were determined in the renal proximal tubule and cortex using real‐time polymerase chain reaction and western blotting techniques, respectively. The results showed higher creatinine clearance and higher urinary excretion of proteins, albumin, and transferrin in the DM group than in the CTRL group. Furthermore, the renal cortex and proximal tubule of diabetic animals showed downregulation of megalin, cubilin, ClC‐5, and CFTR, critical components of the endocytic apparatus. These data suggest dysfunction in proximal tubule low‐molecular‐weight endocytosis and protein Glomerulus filtration in the kidney of diabetic rats.

  • diabetic rats present higher urinary loss of proteins and lower renal expression of megalin cubilin clc 5 and cftr
    Physiological Reports, 2017
    Co-Authors: Miriam Frankenthal Figueira, Raquel C Castiglione, Carolina M L Barbosa, Felipe M Ornellas, Rodrigo Nunes Da Fonseca, Georgia Da Silva Feltran, Marcelo M. Morales, Jackson Souzamenezes
    Abstract:

    Abstract Diabetic nephropathy (DN) occurs in around 40% of those with diabetes. Proteinuria is the main characteristic of DN and develops as a result of increased permeability of the Glomerulus Capillary wall and/or decreased proximal tubule endocytosis. The goal of this work was to evaluate renal function and the expression of megalin, cubilin, CFTR (cystic fibrosis transmembrane conductance regulator), and ClC‐5 in the proximal tubule and renal cortex of rats with type 1 diabetes. Male Wistar rats were randomly assigned to control (CTRL) and diabetic (DM) groups for 4 weeks. Renal function was assessed in 24‐h urine sample by calculating clearance and fractional excretion of solutes. The RNA and protein contents of ClC‐5, CFTR, megalin, and cubilin were determined in the renal proximal tubule and cortex using real‐time polymerase chain reaction and western blotting techniques, respectively. The results showed higher creatinine clearance and higher urinary excretion of proteins, albumin, and transferrin in the DM group than in the CTRL group. Furthermore, the renal cortex and proximal tubule of diabetic animals showed downregulation of megalin, cubilin, ClC‐5, and CFTR, critical components of the endocytic apparatus. These data suggest dysfunction in proximal tubule low‐molecular‐weight endocytosis and protein Glomerulus filtration in the kidney of diabetic rats.

  • Dataset for: Diabetic rats present higher urinary loss of proteins and lower renal expression of megalin, cubilin, ClC-5 and CFTR
    2017
    Co-Authors: Miriam Frankenthal Figueira, Raquel C Castiglione, Felipe M Ornellas, Rodrigo Nunes Da Fonseca, Georgia Da Silva Feltran, Marcelo M. Morales, Carolina Monteiro De Lemos Barbosa, Jackson Souza-menezes
    Abstract:

    Diabetic nephropathy (DN) occurs in around 40% of those with diabetes. Proteinuria is the main characteristic of DN and develops as a result of increased permeability of the Glomerulus Capillary wall and/or decreased proximal tubule endocytosis. The goal of this work was to evaluate renal function and the expression of megalin, cubilin, CFTR (cystic fibrosis transmembrane conductance regulator) and ClC-5 in the proximal tubule and renal cortex of rats with type 1 diabetes. Male Wistar rats were randomly assigned to control (CTRL) and diabetic (DM) groups for 4 weeks. Renal function was assessed in 24-h urine by calculating clearance and fractional excretion of solutes. The RNA and protein content of ClC-5, CFTR, megalin and cubilin was determined in the renal proximal tubule and cortex using real-time polymerase chain reaction and western blotting techniques, respectively. The results showed higher creatinine clearance and higher urinary excretion of proteins, albumin and transferrin in the DM group than in the CTRL group. Furthermore, the renal cortex and proximal tubule of diabetic animals showed downregulation of megalin, cubilin, ClC-5 and CFTR, critical components of the endocytic apparatus. These data suggest dysfunction in proximal tubule low-molecular-weight endocytosis and protein Glomerulus filtration in the kidney of diabetic rats