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Maria Bryszewska - One of the best experts on this subject based on the ideXlab platform.

  • Stability of dendriplexes formed by anti-HIV genetic material and poly(propylene imine) dendrimers in the presence of Glucosaminoglycans.
    The journal of physical chemistry. B, 2012
    Co-Authors: Michal Szewczyk, Joanna Drzewinska, Volha Dzmitruk, Dzmitry Shcharbin, Barbara Klajnert, Dietmar Appelhans, Maria Bryszewska
    Abstract:

    There are several barriers to the application of dendriplexes formed by poly(propylene imine) dendrimers and genetic material for gene therapy. One limitation is their interaction with extracellular matrix components such as Glucosaminoglycans. These can displace the genetic material from the dendriplexes, affecting their transfection activity. In this study, we analyzed the interaction between dendriplexes and the four main Glucosaminoglycans (heparin, heparan sulfate, chondroitin sulfate, and hyaluronic acid) by fluorescence polarization and gel electrophoresis. Dendriplexes were formed by combining three anti-HIV antisense oligodeoxynucleotides with three poly(propylene imine) dendrimers of the fourth generation: unmodified and partially modified with maltose and maltotriose (open shell glycodendrimers). The data showed that the effect of Glucosaminoglycans on dendriplexes depends on the Glucosaminoglycan type and the oligosaccharide serving as the surface group of the dendrimer. Heparin at physiological concentrations destroys dendriplexes formed by open shell glycodendrimers, but dendriplexes based on unmodified poly(propylene imine) dendrimers are stable in its presence. The other Glucosaminoglycans at physiological concentrations cannot destroy dendriplexes formed by any of the dendrimers studied.

  • Stability of Dendriplexes Formed by Anti-HIV Genetic Material and Poly(propylene imine) Dendrimers in the Presence of Glucosaminoglycans
    2012
    Co-Authors: Michal Szewczyk, Joanna Drzewinska, Volha Dzmitruk, Dzmitry Shcharbin, Barbara Klajnert, Dietmar Appelhans, Maria Bryszewska
    Abstract:

    There are several barriers to the application of dendriplexes formed by poly­(propylene imine) dendrimers and genetic material for gene therapy. One limitation is their interaction with extracellular matrix components such as Glucosaminoglycans. These can displace the genetic material from the dendriplexes, affecting their transfection activity. In this study, we analyzed the interaction between dendriplexes and the four main Glucosaminoglycans (heparin, heparan sulfate, chondroitin sulfate, and hyaluronic acid) by fluorescence polarization and gel electrophoresis. Dendriplexes were formed by combining three anti-HIV antisense oligodeoxynucleotides with three poly­(propylene imine) dendrimers of the fourth generation: unmodified and partially modified with maltose and maltotriose (open shell glycodendrimers). The data showed that the effect of Glucosaminoglycans on dendriplexes depends on the Glucosaminoglycan type and the oligosaccharide serving as the surface group of the dendrimer. Heparin at physiological concentrations destroys dendriplexes formed by open shell glycodendrimers, but dendriplexes based on unmodified poly­(propylene imine) dendrimers are stable in its presence. The other Glucosaminoglycans at physiological concentrations cannot destroy dendriplexes formed by any of the dendrimers studied

Michal Szewczyk - One of the best experts on this subject based on the ideXlab platform.

  • Stability of dendriplexes formed by anti-HIV genetic material and poly(propylene imine) dendrimers in the presence of Glucosaminoglycans.
    The journal of physical chemistry. B, 2012
    Co-Authors: Michal Szewczyk, Joanna Drzewinska, Volha Dzmitruk, Dzmitry Shcharbin, Barbara Klajnert, Dietmar Appelhans, Maria Bryszewska
    Abstract:

    There are several barriers to the application of dendriplexes formed by poly(propylene imine) dendrimers and genetic material for gene therapy. One limitation is their interaction with extracellular matrix components such as Glucosaminoglycans. These can displace the genetic material from the dendriplexes, affecting their transfection activity. In this study, we analyzed the interaction between dendriplexes and the four main Glucosaminoglycans (heparin, heparan sulfate, chondroitin sulfate, and hyaluronic acid) by fluorescence polarization and gel electrophoresis. Dendriplexes were formed by combining three anti-HIV antisense oligodeoxynucleotides with three poly(propylene imine) dendrimers of the fourth generation: unmodified and partially modified with maltose and maltotriose (open shell glycodendrimers). The data showed that the effect of Glucosaminoglycans on dendriplexes depends on the Glucosaminoglycan type and the oligosaccharide serving as the surface group of the dendrimer. Heparin at physiological concentrations destroys dendriplexes formed by open shell glycodendrimers, but dendriplexes based on unmodified poly(propylene imine) dendrimers are stable in its presence. The other Glucosaminoglycans at physiological concentrations cannot destroy dendriplexes formed by any of the dendrimers studied.

  • Stability of Dendriplexes Formed by Anti-HIV Genetic Material and Poly(propylene imine) Dendrimers in the Presence of Glucosaminoglycans
    2012
    Co-Authors: Michal Szewczyk, Joanna Drzewinska, Volha Dzmitruk, Dzmitry Shcharbin, Barbara Klajnert, Dietmar Appelhans, Maria Bryszewska
    Abstract:

    There are several barriers to the application of dendriplexes formed by poly­(propylene imine) dendrimers and genetic material for gene therapy. One limitation is their interaction with extracellular matrix components such as Glucosaminoglycans. These can displace the genetic material from the dendriplexes, affecting their transfection activity. In this study, we analyzed the interaction between dendriplexes and the four main Glucosaminoglycans (heparin, heparan sulfate, chondroitin sulfate, and hyaluronic acid) by fluorescence polarization and gel electrophoresis. Dendriplexes were formed by combining three anti-HIV antisense oligodeoxynucleotides with three poly­(propylene imine) dendrimers of the fourth generation: unmodified and partially modified with maltose and maltotriose (open shell glycodendrimers). The data showed that the effect of Glucosaminoglycans on dendriplexes depends on the Glucosaminoglycan type and the oligosaccharide serving as the surface group of the dendrimer. Heparin at physiological concentrations destroys dendriplexes formed by open shell glycodendrimers, but dendriplexes based on unmodified poly­(propylene imine) dendrimers are stable in its presence. The other Glucosaminoglycans at physiological concentrations cannot destroy dendriplexes formed by any of the dendrimers studied

Dietmar Appelhans - One of the best experts on this subject based on the ideXlab platform.

  • Stability of dendriplexes formed by anti-HIV genetic material and poly(propylene imine) dendrimers in the presence of Glucosaminoglycans.
    The journal of physical chemistry. B, 2012
    Co-Authors: Michal Szewczyk, Joanna Drzewinska, Volha Dzmitruk, Dzmitry Shcharbin, Barbara Klajnert, Dietmar Appelhans, Maria Bryszewska
    Abstract:

    There are several barriers to the application of dendriplexes formed by poly(propylene imine) dendrimers and genetic material for gene therapy. One limitation is their interaction with extracellular matrix components such as Glucosaminoglycans. These can displace the genetic material from the dendriplexes, affecting their transfection activity. In this study, we analyzed the interaction between dendriplexes and the four main Glucosaminoglycans (heparin, heparan sulfate, chondroitin sulfate, and hyaluronic acid) by fluorescence polarization and gel electrophoresis. Dendriplexes were formed by combining three anti-HIV antisense oligodeoxynucleotides with three poly(propylene imine) dendrimers of the fourth generation: unmodified and partially modified with maltose and maltotriose (open shell glycodendrimers). The data showed that the effect of Glucosaminoglycans on dendriplexes depends on the Glucosaminoglycan type and the oligosaccharide serving as the surface group of the dendrimer. Heparin at physiological concentrations destroys dendriplexes formed by open shell glycodendrimers, but dendriplexes based on unmodified poly(propylene imine) dendrimers are stable in its presence. The other Glucosaminoglycans at physiological concentrations cannot destroy dendriplexes formed by any of the dendrimers studied.

  • Stability of Dendriplexes Formed by Anti-HIV Genetic Material and Poly(propylene imine) Dendrimers in the Presence of Glucosaminoglycans
    2012
    Co-Authors: Michal Szewczyk, Joanna Drzewinska, Volha Dzmitruk, Dzmitry Shcharbin, Barbara Klajnert, Dietmar Appelhans, Maria Bryszewska
    Abstract:

    There are several barriers to the application of dendriplexes formed by poly­(propylene imine) dendrimers and genetic material for gene therapy. One limitation is their interaction with extracellular matrix components such as Glucosaminoglycans. These can displace the genetic material from the dendriplexes, affecting their transfection activity. In this study, we analyzed the interaction between dendriplexes and the four main Glucosaminoglycans (heparin, heparan sulfate, chondroitin sulfate, and hyaluronic acid) by fluorescence polarization and gel electrophoresis. Dendriplexes were formed by combining three anti-HIV antisense oligodeoxynucleotides with three poly­(propylene imine) dendrimers of the fourth generation: unmodified and partially modified with maltose and maltotriose (open shell glycodendrimers). The data showed that the effect of Glucosaminoglycans on dendriplexes depends on the Glucosaminoglycan type and the oligosaccharide serving as the surface group of the dendrimer. Heparin at physiological concentrations destroys dendriplexes formed by open shell glycodendrimers, but dendriplexes based on unmodified poly­(propylene imine) dendrimers are stable in its presence. The other Glucosaminoglycans at physiological concentrations cannot destroy dendriplexes formed by any of the dendrimers studied

Barbara Klajnert - One of the best experts on this subject based on the ideXlab platform.

  • Stability of dendriplexes formed by anti-HIV genetic material and poly(propylene imine) dendrimers in the presence of Glucosaminoglycans.
    The journal of physical chemistry. B, 2012
    Co-Authors: Michal Szewczyk, Joanna Drzewinska, Volha Dzmitruk, Dzmitry Shcharbin, Barbara Klajnert, Dietmar Appelhans, Maria Bryszewska
    Abstract:

    There are several barriers to the application of dendriplexes formed by poly(propylene imine) dendrimers and genetic material for gene therapy. One limitation is their interaction with extracellular matrix components such as Glucosaminoglycans. These can displace the genetic material from the dendriplexes, affecting their transfection activity. In this study, we analyzed the interaction between dendriplexes and the four main Glucosaminoglycans (heparin, heparan sulfate, chondroitin sulfate, and hyaluronic acid) by fluorescence polarization and gel electrophoresis. Dendriplexes were formed by combining three anti-HIV antisense oligodeoxynucleotides with three poly(propylene imine) dendrimers of the fourth generation: unmodified and partially modified with maltose and maltotriose (open shell glycodendrimers). The data showed that the effect of Glucosaminoglycans on dendriplexes depends on the Glucosaminoglycan type and the oligosaccharide serving as the surface group of the dendrimer. Heparin at physiological concentrations destroys dendriplexes formed by open shell glycodendrimers, but dendriplexes based on unmodified poly(propylene imine) dendrimers are stable in its presence. The other Glucosaminoglycans at physiological concentrations cannot destroy dendriplexes formed by any of the dendrimers studied.

  • Stability of Dendriplexes Formed by Anti-HIV Genetic Material and Poly(propylene imine) Dendrimers in the Presence of Glucosaminoglycans
    2012
    Co-Authors: Michal Szewczyk, Joanna Drzewinska, Volha Dzmitruk, Dzmitry Shcharbin, Barbara Klajnert, Dietmar Appelhans, Maria Bryszewska
    Abstract:

    There are several barriers to the application of dendriplexes formed by poly­(propylene imine) dendrimers and genetic material for gene therapy. One limitation is their interaction with extracellular matrix components such as Glucosaminoglycans. These can displace the genetic material from the dendriplexes, affecting their transfection activity. In this study, we analyzed the interaction between dendriplexes and the four main Glucosaminoglycans (heparin, heparan sulfate, chondroitin sulfate, and hyaluronic acid) by fluorescence polarization and gel electrophoresis. Dendriplexes were formed by combining three anti-HIV antisense oligodeoxynucleotides with three poly­(propylene imine) dendrimers of the fourth generation: unmodified and partially modified with maltose and maltotriose (open shell glycodendrimers). The data showed that the effect of Glucosaminoglycans on dendriplexes depends on the Glucosaminoglycan type and the oligosaccharide serving as the surface group of the dendrimer. Heparin at physiological concentrations destroys dendriplexes formed by open shell glycodendrimers, but dendriplexes based on unmodified poly­(propylene imine) dendrimers are stable in its presence. The other Glucosaminoglycans at physiological concentrations cannot destroy dendriplexes formed by any of the dendrimers studied

Dzmitry Shcharbin - One of the best experts on this subject based on the ideXlab platform.

  • Stability of dendriplexes formed by anti-HIV genetic material and poly(propylene imine) dendrimers in the presence of Glucosaminoglycans.
    The journal of physical chemistry. B, 2012
    Co-Authors: Michal Szewczyk, Joanna Drzewinska, Volha Dzmitruk, Dzmitry Shcharbin, Barbara Klajnert, Dietmar Appelhans, Maria Bryszewska
    Abstract:

    There are several barriers to the application of dendriplexes formed by poly(propylene imine) dendrimers and genetic material for gene therapy. One limitation is their interaction with extracellular matrix components such as Glucosaminoglycans. These can displace the genetic material from the dendriplexes, affecting their transfection activity. In this study, we analyzed the interaction between dendriplexes and the four main Glucosaminoglycans (heparin, heparan sulfate, chondroitin sulfate, and hyaluronic acid) by fluorescence polarization and gel electrophoresis. Dendriplexes were formed by combining three anti-HIV antisense oligodeoxynucleotides with three poly(propylene imine) dendrimers of the fourth generation: unmodified and partially modified with maltose and maltotriose (open shell glycodendrimers). The data showed that the effect of Glucosaminoglycans on dendriplexes depends on the Glucosaminoglycan type and the oligosaccharide serving as the surface group of the dendrimer. Heparin at physiological concentrations destroys dendriplexes formed by open shell glycodendrimers, but dendriplexes based on unmodified poly(propylene imine) dendrimers are stable in its presence. The other Glucosaminoglycans at physiological concentrations cannot destroy dendriplexes formed by any of the dendrimers studied.

  • Stability of Dendriplexes Formed by Anti-HIV Genetic Material and Poly(propylene imine) Dendrimers in the Presence of Glucosaminoglycans
    2012
    Co-Authors: Michal Szewczyk, Joanna Drzewinska, Volha Dzmitruk, Dzmitry Shcharbin, Barbara Klajnert, Dietmar Appelhans, Maria Bryszewska
    Abstract:

    There are several barriers to the application of dendriplexes formed by poly­(propylene imine) dendrimers and genetic material for gene therapy. One limitation is their interaction with extracellular matrix components such as Glucosaminoglycans. These can displace the genetic material from the dendriplexes, affecting their transfection activity. In this study, we analyzed the interaction between dendriplexes and the four main Glucosaminoglycans (heparin, heparan sulfate, chondroitin sulfate, and hyaluronic acid) by fluorescence polarization and gel electrophoresis. Dendriplexes were formed by combining three anti-HIV antisense oligodeoxynucleotides with three poly­(propylene imine) dendrimers of the fourth generation: unmodified and partially modified with maltose and maltotriose (open shell glycodendrimers). The data showed that the effect of Glucosaminoglycans on dendriplexes depends on the Glucosaminoglycan type and the oligosaccharide serving as the surface group of the dendrimer. Heparin at physiological concentrations destroys dendriplexes formed by open shell glycodendrimers, but dendriplexes based on unmodified poly­(propylene imine) dendrimers are stable in its presence. The other Glucosaminoglycans at physiological concentrations cannot destroy dendriplexes formed by any of the dendrimers studied