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Burkhard Schmidt - One of the best experts on this subject based on the ideXlab platform.
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metabolism of the herbicide Glufosinate ammonium in plant cell cultures of transgenic rhizomania resistant and non transgenic sugarbeet beta vulgaris carrot daucus carota purple foxglove digitalis purpurea and thorn apple datura stramonium
Pest Management Science, 2001Co-Authors: Boris P Muller, Andreas Zumdick, Ingolf Schuphan, Burkhard SchmidtAbstract:The metabolism of the herbicide Glufosinate-ammonium was investigated in heterotrophic cell suspension and callus cultures of transgenic (bar-gene) and non-transgenic sugarbeet (Beta vulgaris). Similar studies were performed with suspensions of carrot (Daucus carota), purple foxglove (Digitalis purpurea) and thorn apple (Datura stramonium). 14C-labelled chemicals were the (racemic) Glufosinate, L-Glufosinate, and D-Glufosinate, as well as the metabolites N-acetyl L-Glufosinate and 3-(hydroxymethylphosphinyl)propionic acid (MPP). Cellular absorption was generally low, but depended noticeably on plant species, substance and enantiomer. Portions of non-extractable residues ranged from 0.1% to 1.2% of applied 14C. Amounts of soluble metabolites resulting from Glufosinate or L-Glufosinate were between 0.0% and 26.7% of absorbed 14C in non-transgenic cultures and 28.2% and 59.9% in transgenic sugarbeet. D-Glufosinate, MPP and N-acetyl L-Glufosinate proved to be stable. The main metabolite in transgenic sugarbeet was N-acetyl L-Glufosinate, besides traces of MPP and 4-(hydroxymethylphosphinyl)butanoic acid (MPB). In non-transgenic sugarbeet, Glufosinate was transformed to a limited extent to MPP and trace amounts of MPB. In carrot, D stramonium and D purpurea, MPP was also the main product; MPB was identified as a further trace metabolite in D stramonium and D purpurea. © 2001 Society of Chemical Industry
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metabolism of the herbicide Glufosinate ammonium in plant cell cultures of transgenic rhizomania resistant and non transgenic sugarbeet beta vulgaris carrot daucus carota purple foxglove digitalis purpurea and thorn apple datura stramonium
Pest Management Science, 2001Co-Authors: Boris P Muller, Andreas Zumdick, Ingolf Schuphan, Burkhard SchmidtAbstract:The metabolism of the herbicide Glufosinate-ammonium was investigated in heterotrophic cell suspension and callus cultures of transgenic (bar-gene) and non-transgenic sugarbeet (Beta vulgaris). Similar studies were performed with suspensions of carrot (Daucus carota), purple foxglove (Digitalis purpurea) and thorn apple (Datura stramonium). 14C-labelled chemicals were the (racemic) Glufosinate, L-Glufosinate, and D-Glufosinate, as well as the metabolites N-acetyl L-Glufosinate and 3-(hydroxymethylphosphinyl)propionic acid (MPP). Cellular absorption was generally low, but depended noticeably on plant species, substance and enantiomer. Portions of non-extractable residues ranged from 0.1% to 1.2% of applied 14C. Amounts of soluble metabolites resulting from Glufosinate or L-Glufosinate were between 0.0% and 26.7% of absorbed 14C in non-transgenic cultures and 28.2% and 59.9% in transgenic sugarbeet. D-Glufosinate, MPP and N-acetyl L-Glufosinate proved to be stable. The main metabolite in transgenic sugarbeet was N-acetyl L-Glufosinate, besides traces of MPP and 4-(hydroxymethylphosphinyl)butanoic acid (MPB). In non-transgenic sugarbeet, Glufosinate was transformed to a limited extent to MPP and trace amounts of MPB. In carrot, D stramonium and D purpurea, MPP was also the main product; MPB was identified as a further trace metabolite in D stramonium and D purpurea.
Boris P Muller - One of the best experts on this subject based on the ideXlab platform.
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metabolism of the herbicide Glufosinate ammonium in plant cell cultures of transgenic rhizomania resistant and non transgenic sugarbeet beta vulgaris carrot daucus carota purple foxglove digitalis purpurea and thorn apple datura stramonium
Pest Management Science, 2001Co-Authors: Boris P Muller, Andreas Zumdick, Ingolf Schuphan, Burkhard SchmidtAbstract:The metabolism of the herbicide Glufosinate-ammonium was investigated in heterotrophic cell suspension and callus cultures of transgenic (bar-gene) and non-transgenic sugarbeet (Beta vulgaris). Similar studies were performed with suspensions of carrot (Daucus carota), purple foxglove (Digitalis purpurea) and thorn apple (Datura stramonium). 14C-labelled chemicals were the (racemic) Glufosinate, L-Glufosinate, and D-Glufosinate, as well as the metabolites N-acetyl L-Glufosinate and 3-(hydroxymethylphosphinyl)propionic acid (MPP). Cellular absorption was generally low, but depended noticeably on plant species, substance and enantiomer. Portions of non-extractable residues ranged from 0.1% to 1.2% of applied 14C. Amounts of soluble metabolites resulting from Glufosinate or L-Glufosinate were between 0.0% and 26.7% of absorbed 14C in non-transgenic cultures and 28.2% and 59.9% in transgenic sugarbeet. D-Glufosinate, MPP and N-acetyl L-Glufosinate proved to be stable. The main metabolite in transgenic sugarbeet was N-acetyl L-Glufosinate, besides traces of MPP and 4-(hydroxymethylphosphinyl)butanoic acid (MPB). In non-transgenic sugarbeet, Glufosinate was transformed to a limited extent to MPP and trace amounts of MPB. In carrot, D stramonium and D purpurea, MPP was also the main product; MPB was identified as a further trace metabolite in D stramonium and D purpurea. © 2001 Society of Chemical Industry
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metabolism of the herbicide Glufosinate ammonium in plant cell cultures of transgenic rhizomania resistant and non transgenic sugarbeet beta vulgaris carrot daucus carota purple foxglove digitalis purpurea and thorn apple datura stramonium
Pest Management Science, 2001Co-Authors: Boris P Muller, Andreas Zumdick, Ingolf Schuphan, Burkhard SchmidtAbstract:The metabolism of the herbicide Glufosinate-ammonium was investigated in heterotrophic cell suspension and callus cultures of transgenic (bar-gene) and non-transgenic sugarbeet (Beta vulgaris). Similar studies were performed with suspensions of carrot (Daucus carota), purple foxglove (Digitalis purpurea) and thorn apple (Datura stramonium). 14C-labelled chemicals were the (racemic) Glufosinate, L-Glufosinate, and D-Glufosinate, as well as the metabolites N-acetyl L-Glufosinate and 3-(hydroxymethylphosphinyl)propionic acid (MPP). Cellular absorption was generally low, but depended noticeably on plant species, substance and enantiomer. Portions of non-extractable residues ranged from 0.1% to 1.2% of applied 14C. Amounts of soluble metabolites resulting from Glufosinate or L-Glufosinate were between 0.0% and 26.7% of absorbed 14C in non-transgenic cultures and 28.2% and 59.9% in transgenic sugarbeet. D-Glufosinate, MPP and N-acetyl L-Glufosinate proved to be stable. The main metabolite in transgenic sugarbeet was N-acetyl L-Glufosinate, besides traces of MPP and 4-(hydroxymethylphosphinyl)butanoic acid (MPB). In non-transgenic sugarbeet, Glufosinate was transformed to a limited extent to MPP and trace amounts of MPB. In carrot, D stramonium and D purpurea, MPP was also the main product; MPB was identified as a further trace metabolite in D stramonium and D purpurea.
Ingolf Schuphan - One of the best experts on this subject based on the ideXlab platform.
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metabolism of the herbicide Glufosinate ammonium in plant cell cultures of transgenic rhizomania resistant and non transgenic sugarbeet beta vulgaris carrot daucus carota purple foxglove digitalis purpurea and thorn apple datura stramonium
Pest Management Science, 2001Co-Authors: Boris P Muller, Andreas Zumdick, Ingolf Schuphan, Burkhard SchmidtAbstract:The metabolism of the herbicide Glufosinate-ammonium was investigated in heterotrophic cell suspension and callus cultures of transgenic (bar-gene) and non-transgenic sugarbeet (Beta vulgaris). Similar studies were performed with suspensions of carrot (Daucus carota), purple foxglove (Digitalis purpurea) and thorn apple (Datura stramonium). 14C-labelled chemicals were the (racemic) Glufosinate, L-Glufosinate, and D-Glufosinate, as well as the metabolites N-acetyl L-Glufosinate and 3-(hydroxymethylphosphinyl)propionic acid (MPP). Cellular absorption was generally low, but depended noticeably on plant species, substance and enantiomer. Portions of non-extractable residues ranged from 0.1% to 1.2% of applied 14C. Amounts of soluble metabolites resulting from Glufosinate or L-Glufosinate were between 0.0% and 26.7% of absorbed 14C in non-transgenic cultures and 28.2% and 59.9% in transgenic sugarbeet. D-Glufosinate, MPP and N-acetyl L-Glufosinate proved to be stable. The main metabolite in transgenic sugarbeet was N-acetyl L-Glufosinate, besides traces of MPP and 4-(hydroxymethylphosphinyl)butanoic acid (MPB). In non-transgenic sugarbeet, Glufosinate was transformed to a limited extent to MPP and trace amounts of MPB. In carrot, D stramonium and D purpurea, MPP was also the main product; MPB was identified as a further trace metabolite in D stramonium and D purpurea. © 2001 Society of Chemical Industry
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metabolism of the herbicide Glufosinate ammonium in plant cell cultures of transgenic rhizomania resistant and non transgenic sugarbeet beta vulgaris carrot daucus carota purple foxglove digitalis purpurea and thorn apple datura stramonium
Pest Management Science, 2001Co-Authors: Boris P Muller, Andreas Zumdick, Ingolf Schuphan, Burkhard SchmidtAbstract:The metabolism of the herbicide Glufosinate-ammonium was investigated in heterotrophic cell suspension and callus cultures of transgenic (bar-gene) and non-transgenic sugarbeet (Beta vulgaris). Similar studies were performed with suspensions of carrot (Daucus carota), purple foxglove (Digitalis purpurea) and thorn apple (Datura stramonium). 14C-labelled chemicals were the (racemic) Glufosinate, L-Glufosinate, and D-Glufosinate, as well as the metabolites N-acetyl L-Glufosinate and 3-(hydroxymethylphosphinyl)propionic acid (MPP). Cellular absorption was generally low, but depended noticeably on plant species, substance and enantiomer. Portions of non-extractable residues ranged from 0.1% to 1.2% of applied 14C. Amounts of soluble metabolites resulting from Glufosinate or L-Glufosinate were between 0.0% and 26.7% of absorbed 14C in non-transgenic cultures and 28.2% and 59.9% in transgenic sugarbeet. D-Glufosinate, MPP and N-acetyl L-Glufosinate proved to be stable. The main metabolite in transgenic sugarbeet was N-acetyl L-Glufosinate, besides traces of MPP and 4-(hydroxymethylphosphinyl)butanoic acid (MPB). In non-transgenic sugarbeet, Glufosinate was transformed to a limited extent to MPP and trace amounts of MPB. In carrot, D stramonium and D purpurea, MPP was also the main product; MPB was identified as a further trace metabolite in D stramonium and D purpurea.
Andreas Zumdick - One of the best experts on this subject based on the ideXlab platform.
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metabolism of the herbicide Glufosinate ammonium in plant cell cultures of transgenic rhizomania resistant and non transgenic sugarbeet beta vulgaris carrot daucus carota purple foxglove digitalis purpurea and thorn apple datura stramonium
Pest Management Science, 2001Co-Authors: Boris P Muller, Andreas Zumdick, Ingolf Schuphan, Burkhard SchmidtAbstract:The metabolism of the herbicide Glufosinate-ammonium was investigated in heterotrophic cell suspension and callus cultures of transgenic (bar-gene) and non-transgenic sugarbeet (Beta vulgaris). Similar studies were performed with suspensions of carrot (Daucus carota), purple foxglove (Digitalis purpurea) and thorn apple (Datura stramonium). 14C-labelled chemicals were the (racemic) Glufosinate, L-Glufosinate, and D-Glufosinate, as well as the metabolites N-acetyl L-Glufosinate and 3-(hydroxymethylphosphinyl)propionic acid (MPP). Cellular absorption was generally low, but depended noticeably on plant species, substance and enantiomer. Portions of non-extractable residues ranged from 0.1% to 1.2% of applied 14C. Amounts of soluble metabolites resulting from Glufosinate or L-Glufosinate were between 0.0% and 26.7% of absorbed 14C in non-transgenic cultures and 28.2% and 59.9% in transgenic sugarbeet. D-Glufosinate, MPP and N-acetyl L-Glufosinate proved to be stable. The main metabolite in transgenic sugarbeet was N-acetyl L-Glufosinate, besides traces of MPP and 4-(hydroxymethylphosphinyl)butanoic acid (MPB). In non-transgenic sugarbeet, Glufosinate was transformed to a limited extent to MPP and trace amounts of MPB. In carrot, D stramonium and D purpurea, MPP was also the main product; MPB was identified as a further trace metabolite in D stramonium and D purpurea. © 2001 Society of Chemical Industry
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metabolism of the herbicide Glufosinate ammonium in plant cell cultures of transgenic rhizomania resistant and non transgenic sugarbeet beta vulgaris carrot daucus carota purple foxglove digitalis purpurea and thorn apple datura stramonium
Pest Management Science, 2001Co-Authors: Boris P Muller, Andreas Zumdick, Ingolf Schuphan, Burkhard SchmidtAbstract:The metabolism of the herbicide Glufosinate-ammonium was investigated in heterotrophic cell suspension and callus cultures of transgenic (bar-gene) and non-transgenic sugarbeet (Beta vulgaris). Similar studies were performed with suspensions of carrot (Daucus carota), purple foxglove (Digitalis purpurea) and thorn apple (Datura stramonium). 14C-labelled chemicals were the (racemic) Glufosinate, L-Glufosinate, and D-Glufosinate, as well as the metabolites N-acetyl L-Glufosinate and 3-(hydroxymethylphosphinyl)propionic acid (MPP). Cellular absorption was generally low, but depended noticeably on plant species, substance and enantiomer. Portions of non-extractable residues ranged from 0.1% to 1.2% of applied 14C. Amounts of soluble metabolites resulting from Glufosinate or L-Glufosinate were between 0.0% and 26.7% of absorbed 14C in non-transgenic cultures and 28.2% and 59.9% in transgenic sugarbeet. D-Glufosinate, MPP and N-acetyl L-Glufosinate proved to be stable. The main metabolite in transgenic sugarbeet was N-acetyl L-Glufosinate, besides traces of MPP and 4-(hydroxymethylphosphinyl)butanoic acid (MPB). In non-transgenic sugarbeet, Glufosinate was transformed to a limited extent to MPP and trace amounts of MPB. In carrot, D stramonium and D purpurea, MPP was also the main product; MPB was identified as a further trace metabolite in D stramonium and D purpurea.
Pierre Leroux - One of the best experts on this subject based on the ideXlab platform.
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influence du ph d acides amines et de diverses substances organiques sur la fongitoxicite du pyrimethanil du Glufosinate du captafol du cymoxanil et du fenpiclonil vis a vis de certaines souches de botrytis cinerea
Agronomie, 1994Co-Authors: Pierre LerouxAbstract:L'effet du pyrimethanil, du fenpiclonil, du captafol, du Glufosinate et du cymoxanil sur l'elongation des filaments germinatifs des spores de Botrytis cinerea est etudie dans diverses conditions culturales du champignon. Si le fenpiclonil presente un niveau d'activite stable entre pH 3,6 et 7,3, en revanche les autres pesticides voient leur toxicite chuter dans des milieux acides (ex pyrimethanil) ou neutres a faiblement basiques (ex captafol, cymoxanil, Glufosinate). L'adjonction d'extrait de levure, de peptone ou d'hydrolysat de caseine reduit notablement l'activite du pyrimethanil, du captafol, du Glufosinate et du cymoxanil mais pas celle du fenpiclonil. Une analyse detaillee des acides amines indique que ceux possedant un atome de soufre sont plus ou moins antagonistes du pyrimethanil, du cymoxanil, du Glufosinate et du captafol. Si, pour les 3 premiers pesticides, cet effet protecteur pourrait etre correle avec une inhibition de la biosynthese d'acides amines soufres, en revanche, pour le captafol c'est une detoxication par les groupements thiols qui intervient. Parmi les autres acides amines entrainant un net antagonisme il y a la glutamine vis-a-vis du Glufosinate, la valine et la leucine vis-a-vis du pyrimethanil ou la glycine et la serine vis-a-vis du cymoxanil. Les relations entre les effets protecteurs des acides amines et le mode d'action de ces pesticides chez B cinerea sont evoquees. Les implications de ces resultats dans l'elaboration de methodes de surveillance de la resistance a certains nouveaux fongicides (ex fenpiclonil, pyrimethanil) sont egalement discutees
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Influence du pH, d'acides aminés et de diverses substances organiques sur la fongitoxicité du pyriméthanil, du Glufosinate, du captafol, du cymoxanil et du fenpiclonil vis-à-vis de certaines souches de Botrytis cinerea
EDP Sciences, 1994Co-Authors: Pierre LerouxAbstract:L'effet du pyriméthanil, du fenpiclonil, du captafol, du Glufosinate et du cymoxanil sur l'élongation des filaments germinatifs des spores de Botrytis cinerea est étudié dans diverses conditions culturales du champignon. Si le fenpiclonil présente un niveau d'activité stable entre pH 3,6 et 7,3, en revanche les autres pesticides voient leur toxicité chuter dans des milieux acides (ex pyriméthanil) ou neutres à faiblement basiques (ex captafol, cymoxanil, Glufosinate). L'adjonction d'extrait de levure, de peptone ou d'hydrolysat de caséine réduit notablement l'activité du pyriméthanil, du captafol, du Glufosinate et du cymoxanil mais pas celle du fenpiclonil. Une analyse détaillée des acides aminés indique que ceux possédant un atome de soufre sont plus ou moins antagonistes du pyriméthanil, du cymoxanil, du Glufosinate et du captafol. Si, pour les 3 premiers pesticides, cet effet protecteur pourrait être corrélé avec une inhibition de la biosynthèse d'acides aminés soufrés, en revanche, pour le captafol c'est une détoxication par les groupements thiols qui intervient. Parmi les autres acides aminés entraînant un net antagonisme il y a la glutamine vis-à-vis du Glufosinate, la valine et la leucine vis-à-vis du pyriméthanil ou la glycine et la sérine vis-à-vis du cymoxanil. Les relations entre les effets protecteurs des acides aminés et le mode d'action de ces pesticides chez B cinerea sont évoquées. Les implications de ces résultats dans l'élaboration de méthodes de surveillance de la résistance à certains nouveaux fongicides (ex fenpiclonil, pyriméthanil) sont également discutées.Effect of pH, amino acids and various organic compounds on the fungitoxicity of pyrimethanil, Glufosinate, captafol, cymoxanil and fenpiclonil in Botrytis cinerea. The inhibition of germ-tube elongation by pyrimethanil, fenpiclonil, captafol, Glufosinate and cymoxanil was studied in Botrytis cinerea cultivated on various media. The activity of fenpiclonil was not influenced by pH (between 3.6 and 7.3) whereas that of the other pesticides was reduced either in acidic conditions (eg, pyrimethanil) or at pH values greater than 6 (eg, Glufosinate, captafol, cymoxanil). The addition of yeast extract, peptone or casein-hydrolysate relieved pyrimethanil, Glufosinate, cymoxanil and captafol inhibition but not that of fenpiclonil. A detailed study conducted with amino acids indicated that those containing sulphur were antagonistic towards pyrimethanil, cymoxanil, Glufosinate and captafol. For the first 3 pesticides, this reversal could be related to an inhibition in the biosynthesis of sulphur-containing amino acids whereas for captafol it was probably due to its reaction with thiols. Among the other amino acids that exhibit alleviatory effects we can mention glutamine for Glufosinate, valine and leucine for pyrimethanil or glycine, and serine for cymoxanil. The relationship between the antagonistic effects of amino acids and the biochemical mode of action of the tested pesticides in B cinerea are evoked. The consequences of the results upon the development of suitable methods for fungicide-resistance monitoring are also discussed