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P H Rosenberg - One of the best experts on this subject based on the ideXlab platform.

  • hepatocellular integrity in liver donors and recipients indicated by Glutathione Transferase Alpha
    Transplantation, 1996
    Co-Authors: P Tiainen, K Hockerstedt, P H Rosenberg
    Abstract:

    Glutathione Transferase Alpha (GSTA) is a sensitive indicator of hepatocellular integrity. Its reference range is low (0.7-14 microgram/L) and its half-life is short (1 hr) in serum. We evaluated the changes in GSTA concentration in 18 recipients during and after liver transplantation. The respective liver donors were also included in 13 cases. The baseline GSTA concentrations were normal or slightly elevated in all donors, 1.2-79 micrograms/L (median 5.1 micrograms/L) and recipients, 1.1-34 micrograms/L (median 6.4 micrograms/L). Surgical dissection of donor liver caused a moderate or even large increase in GSTA concentration, peak 80-6500 microgram/L (median 800 micrograms/L). In the recipients the peak of GSTA concentrations varied from 1400 to 47000 micrograms/L (median 5000 micrograms/L), and it was always observed within 45 min after reperfusion of the graft. The highest GSTA values were observed after long cold ischemia and in patients transplanted for acute liver failure. However, they were not associated with early graft dysfunction. There was a correlation between the AUC of GSTA and cold ischemia time in the recipients with chronic nonalcoholic liver failure (r=0.94). There was no correlation between GSTA values in the donors and recipients (r=0.14). The apparent half-life of GSTA in serum was 56 min (median). Perioperative GSTA concentrations in the donors had no obvious predictive value. In the recipients an exceptionally long apparent half-life of GSTA immediately after transplantation or a large second increase in GSTA were predictors of postoperative complications.

  • comparison of the effects of sevoflurane and halothane on the quality of anaesthesia and serum Glutathione Transferase Alpha and fluoride in paediatric patients
    BJA: British Journal of Anaesthesia, 1994
    Co-Authors: T Taivainen, P Tiainen, O A Meretoja, L Raiha, P H Rosenberg
    Abstract:

    We have compared sevoflurane and halothane anaesthesia in paediatric patients with reference to induction and recovery. We also assessed hepato-cellular integrity by measurement of serum gluta-thione Transferase Alpha (GSTA) concentration and sevoflurane metabolism by serum fluoride concentration. Fifty unpremedicated 5–12-yr-old children were allocated randomly to induction of anaesthesia via a face mask with 66% nitrous oxide in oxygen and sevoflurane (up to 7%) or halothane (up to 3.5%). Anaesthesia was maintained for 1.8 h at 1–1.2 MAC of the volatile agent. Children receiving sevoflurane had significantly faster induction and recovery variables than those receiving halothane. There was a small postanaesthetic increase in GSTA in both groups, suggesting that halothane and sevoflurane may disturb hepato-cellular integrity. Serum concentrations of fluoride were significantly greater after sevoflurane than after halothane anaesthesia. There were no clinical signs or symptoms of hepatic or renal disturbance. Children tolerated sevoflurane better than halothane, which may have been because of the non-pungency of sevoflurane and the rapid psycho-motor recovery after anaesthesia.

K K Karhi - One of the best experts on this subject based on the ideXlab platform.

  • ultrasensitive time resolved immunofluorometric assay of Glutathione Transferase Alpha in serum
    Clinical Chemistry, 1994
    Co-Authors: Pekka Tiainen, K K Karhi
    Abstract:

    Glutathione Transferase Alpha (GSTA) is a very sensitive marker of acute centrilobular liver damage. We purified Glutathione Transferases from human liver and separated the isoenzymes. Polyclonal rabbit antibodies specific for Alpha-class isoenzymes were produced and labeled with Eu(3+)-chelate. We set up a sandwich-type time-resolved immunofluorometric assay (TR-IFMA) to measure GSTA in serum. The detection limit was 0.03 microgram/L, and measuring range was from 0.03 to 100 micrograms/L. The reference range for serum GSTA in this assay was 0.7-6.0 micrograms/L for women and 0.7-14 micrograms/L for men. This TR-IFMA is practical, and the modified rapid assay can be completed in 3 h. Therefore it is applicable for diagnostic purposes in acute liver damage, e.g., associated with drug toxicity or hypoperfusion of the liver.

Bengt Mannervik - One of the best experts on this subject based on the ideXlab platform.

  • porcine Glutathione Transferase Alpha 2 2 is a human gst a3 3 analogue that catalyses steroid double bond isomerization
    Biochemical Journal, 2010
    Co-Authors: Natalia Fedulova, Francoise Raffallimathieu, Bengt Mannervik
    Abstract:

    A primary role of GSTs (Glutathione Transferases) is detoxication of electrophilic compounds. In addition to this protective function, hGST (human GST) A3-3, a member of the Alpha class of soluble GSTs, has prominent steroid double-bond isomerase activity. The isomerase reaction is an obligatory step in the biosynthesis of steroid hormones, indicating a special role of hGST A3-3 in steroidogenic tissues. An analogous GST with high steroid isomerase activity has so far not been found in any other biological species. In the present study, we characterized a Sus scrofa (pig) enzyme, pGST A2-2, displaying high steroid isomerase activity. High levels of pGST A2-2 expression were found in ovary, testis and liver. In its functional properties, other than steroid isomerization, pGST A2-2 was most similar to hGST A3-3. The properties of the novel porcine enzyme lend support to the notion that particular GSTs play an important role in steroidogenesis.

P Tiainen - One of the best experts on this subject based on the ideXlab platform.

  • hepatocellular integrity in liver donors and recipients indicated by Glutathione Transferase Alpha
    Transplantation, 1996
    Co-Authors: P Tiainen, K Hockerstedt, P H Rosenberg
    Abstract:

    Glutathione Transferase Alpha (GSTA) is a sensitive indicator of hepatocellular integrity. Its reference range is low (0.7-14 microgram/L) and its half-life is short (1 hr) in serum. We evaluated the changes in GSTA concentration in 18 recipients during and after liver transplantation. The respective liver donors were also included in 13 cases. The baseline GSTA concentrations were normal or slightly elevated in all donors, 1.2-79 micrograms/L (median 5.1 micrograms/L) and recipients, 1.1-34 micrograms/L (median 6.4 micrograms/L). Surgical dissection of donor liver caused a moderate or even large increase in GSTA concentration, peak 80-6500 microgram/L (median 800 micrograms/L). In the recipients the peak of GSTA concentrations varied from 1400 to 47000 micrograms/L (median 5000 micrograms/L), and it was always observed within 45 min after reperfusion of the graft. The highest GSTA values were observed after long cold ischemia and in patients transplanted for acute liver failure. However, they were not associated with early graft dysfunction. There was a correlation between the AUC of GSTA and cold ischemia time in the recipients with chronic nonalcoholic liver failure (r=0.94). There was no correlation between GSTA values in the donors and recipients (r=0.14). The apparent half-life of GSTA in serum was 56 min (median). Perioperative GSTA concentrations in the donors had no obvious predictive value. In the recipients an exceptionally long apparent half-life of GSTA immediately after transplantation or a large second increase in GSTA were predictors of postoperative complications.

  • comparison of the effects of sevoflurane and halothane on the quality of anaesthesia and serum Glutathione Transferase Alpha and fluoride in paediatric patients
    BJA: British Journal of Anaesthesia, 1994
    Co-Authors: T Taivainen, P Tiainen, O A Meretoja, L Raiha, P H Rosenberg
    Abstract:

    We have compared sevoflurane and halothane anaesthesia in paediatric patients with reference to induction and recovery. We also assessed hepato-cellular integrity by measurement of serum gluta-thione Transferase Alpha (GSTA) concentration and sevoflurane metabolism by serum fluoride concentration. Fifty unpremedicated 5–12-yr-old children were allocated randomly to induction of anaesthesia via a face mask with 66% nitrous oxide in oxygen and sevoflurane (up to 7%) or halothane (up to 3.5%). Anaesthesia was maintained for 1.8 h at 1–1.2 MAC of the volatile agent. Children receiving sevoflurane had significantly faster induction and recovery variables than those receiving halothane. There was a small postanaesthetic increase in GSTA in both groups, suggesting that halothane and sevoflurane may disturb hepato-cellular integrity. Serum concentrations of fluoride were significantly greater after sevoflurane than after halothane anaesthesia. There were no clinical signs or symptoms of hepatic or renal disturbance. Children tolerated sevoflurane better than halothane, which may have been because of the non-pungency of sevoflurane and the rapid psycho-motor recovery after anaesthesia.

Pekka Tiainen - One of the best experts on this subject based on the ideXlab platform.

  • ultrasensitive time resolved immunofluorometric assay of Glutathione Transferase Alpha in serum
    Clinical Chemistry, 1994
    Co-Authors: Pekka Tiainen, K K Karhi
    Abstract:

    Glutathione Transferase Alpha (GSTA) is a very sensitive marker of acute centrilobular liver damage. We purified Glutathione Transferases from human liver and separated the isoenzymes. Polyclonal rabbit antibodies specific for Alpha-class isoenzymes were produced and labeled with Eu(3+)-chelate. We set up a sandwich-type time-resolved immunofluorometric assay (TR-IFMA) to measure GSTA in serum. The detection limit was 0.03 microgram/L, and measuring range was from 0.03 to 100 micrograms/L. The reference range for serum GSTA in this assay was 0.7-6.0 micrograms/L for women and 0.7-14 micrograms/L for men. This TR-IFMA is practical, and the modified rapid assay can be completed in 3 h. Therefore it is applicable for diagnostic purposes in acute liver damage, e.g., associated with drug toxicity or hypoperfusion of the liver.