The Experts below are selected from a list of 138 Experts worldwide ranked by ideXlab platform
Michael J Conlon - One of the best experts on this subject based on the ideXlab platform.
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the alyteserins two families of antimicrobial peptides from the skin secretions of the midwife toad alytes obstetricans alytidae
Peptides, 2009Co-Authors: Michael J Conlon, Anni Demandt, Per F. Nielsen, Hubert Vaudry, Jérôme Leprince, Douglas C. WoodhamsAbstract:Abstract Two families of structurally related C-terminally α-amidated antimicrobial peptides have been identified in norepinephrine-stimulated skin secretions of the midwife toad Alytes obstetricans (Alytidae). The alyteserin-1 peptides (Gly-Leu-Lys-(Asp/Glu)-Ile-Phe-Lys-Ala-Gly-Leu-Gly-Ser-Leu-Val-Lys-(Gly/Asn)-Ile-Ala-Ala-His-Val-Ala-(Asn/Ser).NH 2 ) show limited structural similarity to the ascaphins from the skins of frogs of the family Leiopelmatidae. Alyteserin-2a (Ile-Leu-Gly-Lys-Leu-Leu-Ser-Thr-Ala-Ala-Gly-Leu-Leu-Ser-Asn-Leu.NH 2 ) and alyteserin-2b and -2c (Ile-Leu-Gly-Ala-Ile-Leu-Pro-Leu-Val-Ser-Gly-Leu-Leu-Ser-(Asn/Ser)-Lys-Leu.NH 2 ) show limited sequence identity with bombinin H6, present in the skins of frogs of the family Bombinatoridae. The alyteserin-1 peptides show selective growth inhibitory activity against the Gram-negative bacteria Escherichia coli (MIC = 25 μM) whereas alyteserin-2a is more potent against the Gram-positive bacteria Staphylococcus aureus (MIC = 50 μM). The hemolytic activity against human erythrocytes of all peptides tested is relatively weak (LC 50 > 100 μM). The data demonstrate that the frogs belonging to the family Alytidae are among those producing dermal antimicrobial peptides that may represent a component of the animal's system of innate immunity.
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purification and characterization of galanin and scyliorhinin i from the hybrid sturgeon scaphirhynchus platorynchus scaphirhynchus albus acipenseriformes
General and Comparative Endocrinology, 1999Co-Authors: Yuqi Wang, Per F. Nielsen, Bruce A Barton, Michael J ConlonAbstract:Abstract The sturgeons (order Acipenseriformes) are extant representatives of a group of ancient Actinopterygian (ray-finned) fish. Galanin and scyliorhinin I (a tachykinin with limited structural similarity to mammalian substance P) have been isolated from an extract of the gastrointestinal tract of a sturgeon (an F1 hybrid between the shovelnose sturgeon, Scaphirhynchus platorynchus, and the pallid sturgeon, Scaphirhynchus albus ). The primary structure of sturgeon galanin (Gly-Trp-Thr-Leu-Asn-Ser-Ala-Gly-Tyr-Leu 10 -Leu-Gly-Pro-His-Ala-Val-Asp-Gly-His-Arg 20 -Ser-Leu-Ser-Asp-Lys-His-Gly-Leu-Pro·NH 2 ) contains only two amino acid substitutions (Ser 23 → Asn and Pro 29 → Ala) compared with galanin from the bowfin, Amia calva (Amiiformes), but five amino acid substitutions compared with galanin from the trout (Teleostei). Similarly, the sturgeon tachykinin (Ser-Lys-Tyr-His-Gln-Phe-Tyr-Gly-Leu-Met·NH 2 ) contains only one amino acid substitution (Tyr 3 → Ser) compared with scyliorhinin I previously isolated from bowfin stomach but five amino acid substitutions compared with trout substance P. The data support the hypothesis that the Acipenseriformes and the basal Neopterygians (gars and bowfin) share a close phylogenetic relationship.
Per F. Nielsen - One of the best experts on this subject based on the ideXlab platform.
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the alyteserins two families of antimicrobial peptides from the skin secretions of the midwife toad alytes obstetricans alytidae
Peptides, 2009Co-Authors: Michael J Conlon, Anni Demandt, Per F. Nielsen, Hubert Vaudry, Jérôme Leprince, Douglas C. WoodhamsAbstract:Abstract Two families of structurally related C-terminally α-amidated antimicrobial peptides have been identified in norepinephrine-stimulated skin secretions of the midwife toad Alytes obstetricans (Alytidae). The alyteserin-1 peptides (Gly-Leu-Lys-(Asp/Glu)-Ile-Phe-Lys-Ala-Gly-Leu-Gly-Ser-Leu-Val-Lys-(Gly/Asn)-Ile-Ala-Ala-His-Val-Ala-(Asn/Ser).NH 2 ) show limited structural similarity to the ascaphins from the skins of frogs of the family Leiopelmatidae. Alyteserin-2a (Ile-Leu-Gly-Lys-Leu-Leu-Ser-Thr-Ala-Ala-Gly-Leu-Leu-Ser-Asn-Leu.NH 2 ) and alyteserin-2b and -2c (Ile-Leu-Gly-Ala-Ile-Leu-Pro-Leu-Val-Ser-Gly-Leu-Leu-Ser-(Asn/Ser)-Lys-Leu.NH 2 ) show limited sequence identity with bombinin H6, present in the skins of frogs of the family Bombinatoridae. The alyteserin-1 peptides show selective growth inhibitory activity against the Gram-negative bacteria Escherichia coli (MIC = 25 μM) whereas alyteserin-2a is more potent against the Gram-positive bacteria Staphylococcus aureus (MIC = 50 μM). The hemolytic activity against human erythrocytes of all peptides tested is relatively weak (LC 50 > 100 μM). The data demonstrate that the frogs belonging to the family Alytidae are among those producing dermal antimicrobial peptides that may represent a component of the animal's system of innate immunity.
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Purification and Characterization of Galanin and Scyliorhinin I from the Hybrid Sturgeon,Scaphirhynchus platorynchus×Scaphirhynchus albus(Acipenseriformes) ☆
General and Comparative Endocrinology, 1999Co-Authors: Yuqi Wang, Per F. Nielsen, Bruce A Barton, J. Michael ConlonAbstract:Abstract The sturgeons (order Acipenseriformes) are extant representatives of a group of ancient Actinopterygian (ray-finned) fish. Galanin and scyliorhinin I (a tachykinin with limited structural similarity to mammalian substance P) have been isolated from an extract of the gastrointestinal tract of a sturgeon (an F1 hybrid between the shovelnose sturgeon, Scaphirhynchus platorynchus, and the pallid sturgeon, Scaphirhynchus albus ). The primary structure of sturgeon galanin (Gly-Trp-Thr-Leu-Asn-Ser-Ala-Gly-Tyr-Leu 10 -Leu-Gly-Pro-His-Ala-Val-Asp-Gly-His-Arg 20 -Ser-Leu-Ser-Asp-Lys-His-Gly-Leu-Pro·NH 2 ) contains only two amino acid substitutions (Ser 23 → Asn and Pro 29 → Ala) compared with galanin from the bowfin, Amia calva (Amiiformes), but five amino acid substitutions compared with galanin from the trout (Teleostei). Similarly, the sturgeon tachykinin (Ser-Lys-Tyr-His-Gln-Phe-Tyr-Gly-Leu-Met·NH 2 ) contains only one amino acid substitution (Tyr 3 → Ser) compared with scyliorhinin I previously isolated from bowfin stomach but five amino acid substitutions compared with trout substance P. The data support the hypothesis that the Acipenseriformes and the basal Neopterygians (gars and bowfin) share a close phylogenetic relationship.
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purification and characterization of galanin and scyliorhinin i from the hybrid sturgeon scaphirhynchus platorynchus scaphirhynchus albus acipenseriformes
General and Comparative Endocrinology, 1999Co-Authors: Yuqi Wang, Per F. Nielsen, Bruce A Barton, Michael J ConlonAbstract:Abstract The sturgeons (order Acipenseriformes) are extant representatives of a group of ancient Actinopterygian (ray-finned) fish. Galanin and scyliorhinin I (a tachykinin with limited structural similarity to mammalian substance P) have been isolated from an extract of the gastrointestinal tract of a sturgeon (an F1 hybrid between the shovelnose sturgeon, Scaphirhynchus platorynchus, and the pallid sturgeon, Scaphirhynchus albus ). The primary structure of sturgeon galanin (Gly-Trp-Thr-Leu-Asn-Ser-Ala-Gly-Tyr-Leu 10 -Leu-Gly-Pro-His-Ala-Val-Asp-Gly-His-Arg 20 -Ser-Leu-Ser-Asp-Lys-His-Gly-Leu-Pro·NH 2 ) contains only two amino acid substitutions (Ser 23 → Asn and Pro 29 → Ala) compared with galanin from the bowfin, Amia calva (Amiiformes), but five amino acid substitutions compared with galanin from the trout (Teleostei). Similarly, the sturgeon tachykinin (Ser-Lys-Tyr-His-Gln-Phe-Tyr-Gly-Leu-Met·NH 2 ) contains only one amino acid substitution (Tyr 3 → Ser) compared with scyliorhinin I previously isolated from bowfin stomach but five amino acid substitutions compared with trout substance P. The data support the hypothesis that the Acipenseriformes and the basal Neopterygians (gars and bowfin) share a close phylogenetic relationship.
Yuqi Wang - One of the best experts on this subject based on the ideXlab platform.
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Purification and Characterization of Galanin and Scyliorhinin I from the Hybrid Sturgeon,Scaphirhynchus platorynchus×Scaphirhynchus albus(Acipenseriformes) ☆
General and Comparative Endocrinology, 1999Co-Authors: Yuqi Wang, Per F. Nielsen, Bruce A Barton, J. Michael ConlonAbstract:Abstract The sturgeons (order Acipenseriformes) are extant representatives of a group of ancient Actinopterygian (ray-finned) fish. Galanin and scyliorhinin I (a tachykinin with limited structural similarity to mammalian substance P) have been isolated from an extract of the gastrointestinal tract of a sturgeon (an F1 hybrid between the shovelnose sturgeon, Scaphirhynchus platorynchus, and the pallid sturgeon, Scaphirhynchus albus ). The primary structure of sturgeon galanin (Gly-Trp-Thr-Leu-Asn-Ser-Ala-Gly-Tyr-Leu 10 -Leu-Gly-Pro-His-Ala-Val-Asp-Gly-His-Arg 20 -Ser-Leu-Ser-Asp-Lys-His-Gly-Leu-Pro·NH 2 ) contains only two amino acid substitutions (Ser 23 → Asn and Pro 29 → Ala) compared with galanin from the bowfin, Amia calva (Amiiformes), but five amino acid substitutions compared with galanin from the trout (Teleostei). Similarly, the sturgeon tachykinin (Ser-Lys-Tyr-His-Gln-Phe-Tyr-Gly-Leu-Met·NH 2 ) contains only one amino acid substitution (Tyr 3 → Ser) compared with scyliorhinin I previously isolated from bowfin stomach but five amino acid substitutions compared with trout substance P. The data support the hypothesis that the Acipenseriformes and the basal Neopterygians (gars and bowfin) share a close phylogenetic relationship.
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purification and characterization of galanin and scyliorhinin i from the hybrid sturgeon scaphirhynchus platorynchus scaphirhynchus albus acipenseriformes
General and Comparative Endocrinology, 1999Co-Authors: Yuqi Wang, Per F. Nielsen, Bruce A Barton, Michael J ConlonAbstract:Abstract The sturgeons (order Acipenseriformes) are extant representatives of a group of ancient Actinopterygian (ray-finned) fish. Galanin and scyliorhinin I (a tachykinin with limited structural similarity to mammalian substance P) have been isolated from an extract of the gastrointestinal tract of a sturgeon (an F1 hybrid between the shovelnose sturgeon, Scaphirhynchus platorynchus, and the pallid sturgeon, Scaphirhynchus albus ). The primary structure of sturgeon galanin (Gly-Trp-Thr-Leu-Asn-Ser-Ala-Gly-Tyr-Leu 10 -Leu-Gly-Pro-His-Ala-Val-Asp-Gly-His-Arg 20 -Ser-Leu-Ser-Asp-Lys-His-Gly-Leu-Pro·NH 2 ) contains only two amino acid substitutions (Ser 23 → Asn and Pro 29 → Ala) compared with galanin from the bowfin, Amia calva (Amiiformes), but five amino acid substitutions compared with galanin from the trout (Teleostei). Similarly, the sturgeon tachykinin (Ser-Lys-Tyr-His-Gln-Phe-Tyr-Gly-Leu-Met·NH 2 ) contains only one amino acid substitution (Tyr 3 → Ser) compared with scyliorhinin I previously isolated from bowfin stomach but five amino acid substitutions compared with trout substance P. The data support the hypothesis that the Acipenseriformes and the basal Neopterygians (gars and bowfin) share a close phylogenetic relationship.
J. Michael Conlon - One of the best experts on this subject based on the ideXlab platform.
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Purification and Characterization of Galanin and Scyliorhinin I from the Hybrid Sturgeon,Scaphirhynchus platorynchus×Scaphirhynchus albus(Acipenseriformes) ☆
General and Comparative Endocrinology, 1999Co-Authors: Yuqi Wang, Per F. Nielsen, Bruce A Barton, J. Michael ConlonAbstract:Abstract The sturgeons (order Acipenseriformes) are extant representatives of a group of ancient Actinopterygian (ray-finned) fish. Galanin and scyliorhinin I (a tachykinin with limited structural similarity to mammalian substance P) have been isolated from an extract of the gastrointestinal tract of a sturgeon (an F1 hybrid between the shovelnose sturgeon, Scaphirhynchus platorynchus, and the pallid sturgeon, Scaphirhynchus albus ). The primary structure of sturgeon galanin (Gly-Trp-Thr-Leu-Asn-Ser-Ala-Gly-Tyr-Leu 10 -Leu-Gly-Pro-His-Ala-Val-Asp-Gly-His-Arg 20 -Ser-Leu-Ser-Asp-Lys-His-Gly-Leu-Pro·NH 2 ) contains only two amino acid substitutions (Ser 23 → Asn and Pro 29 → Ala) compared with galanin from the bowfin, Amia calva (Amiiformes), but five amino acid substitutions compared with galanin from the trout (Teleostei). Similarly, the sturgeon tachykinin (Ser-Lys-Tyr-His-Gln-Phe-Tyr-Gly-Leu-Met·NH 2 ) contains only one amino acid substitution (Tyr 3 → Ser) compared with scyliorhinin I previously isolated from bowfin stomach but five amino acid substitutions compared with trout substance P. The data support the hypothesis that the Acipenseriformes and the basal Neopterygians (gars and bowfin) share a close phylogenetic relationship.
Bruce A Barton - One of the best experts on this subject based on the ideXlab platform.
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Purification and Characterization of Galanin and Scyliorhinin I from the Hybrid Sturgeon,Scaphirhynchus platorynchus×Scaphirhynchus albus(Acipenseriformes) ☆
General and Comparative Endocrinology, 1999Co-Authors: Yuqi Wang, Per F. Nielsen, Bruce A Barton, J. Michael ConlonAbstract:Abstract The sturgeons (order Acipenseriformes) are extant representatives of a group of ancient Actinopterygian (ray-finned) fish. Galanin and scyliorhinin I (a tachykinin with limited structural similarity to mammalian substance P) have been isolated from an extract of the gastrointestinal tract of a sturgeon (an F1 hybrid between the shovelnose sturgeon, Scaphirhynchus platorynchus, and the pallid sturgeon, Scaphirhynchus albus ). The primary structure of sturgeon galanin (Gly-Trp-Thr-Leu-Asn-Ser-Ala-Gly-Tyr-Leu 10 -Leu-Gly-Pro-His-Ala-Val-Asp-Gly-His-Arg 20 -Ser-Leu-Ser-Asp-Lys-His-Gly-Leu-Pro·NH 2 ) contains only two amino acid substitutions (Ser 23 → Asn and Pro 29 → Ala) compared with galanin from the bowfin, Amia calva (Amiiformes), but five amino acid substitutions compared with galanin from the trout (Teleostei). Similarly, the sturgeon tachykinin (Ser-Lys-Tyr-His-Gln-Phe-Tyr-Gly-Leu-Met·NH 2 ) contains only one amino acid substitution (Tyr 3 → Ser) compared with scyliorhinin I previously isolated from bowfin stomach but five amino acid substitutions compared with trout substance P. The data support the hypothesis that the Acipenseriformes and the basal Neopterygians (gars and bowfin) share a close phylogenetic relationship.
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purification and characterization of galanin and scyliorhinin i from the hybrid sturgeon scaphirhynchus platorynchus scaphirhynchus albus acipenseriformes
General and Comparative Endocrinology, 1999Co-Authors: Yuqi Wang, Per F. Nielsen, Bruce A Barton, Michael J ConlonAbstract:Abstract The sturgeons (order Acipenseriformes) are extant representatives of a group of ancient Actinopterygian (ray-finned) fish. Galanin and scyliorhinin I (a tachykinin with limited structural similarity to mammalian substance P) have been isolated from an extract of the gastrointestinal tract of a sturgeon (an F1 hybrid between the shovelnose sturgeon, Scaphirhynchus platorynchus, and the pallid sturgeon, Scaphirhynchus albus ). The primary structure of sturgeon galanin (Gly-Trp-Thr-Leu-Asn-Ser-Ala-Gly-Tyr-Leu 10 -Leu-Gly-Pro-His-Ala-Val-Asp-Gly-His-Arg 20 -Ser-Leu-Ser-Asp-Lys-His-Gly-Leu-Pro·NH 2 ) contains only two amino acid substitutions (Ser 23 → Asn and Pro 29 → Ala) compared with galanin from the bowfin, Amia calva (Amiiformes), but five amino acid substitutions compared with galanin from the trout (Teleostei). Similarly, the sturgeon tachykinin (Ser-Lys-Tyr-His-Gln-Phe-Tyr-Gly-Leu-Met·NH 2 ) contains only one amino acid substitution (Tyr 3 → Ser) compared with scyliorhinin I previously isolated from bowfin stomach but five amino acid substitutions compared with trout substance P. The data support the hypothesis that the Acipenseriformes and the basal Neopterygians (gars and bowfin) share a close phylogenetic relationship.