The Experts below are selected from a list of 489 Experts worldwide ranked by ideXlab platform
Toshimasa Nishiyama - One of the best experts on this subject based on the ideXlab platform.
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the in vitro estrogenic activity of the crude drugs found in japanese herbal medicines prescribed for menopausal syndrome was enhanced by combining them
BMC Complementary and Alternative Medicine, 2018Co-Authors: Zeyun Wang, Dambajamts Enkhundraa, Takaki Shimono, Seiji Kanda, Toshimasa NishiyamaAbstract:Japanese herbal medicines can be used as alternatives to estrogen therapy and are sometimes prescribed for menopausal syndrome because they have fewer side effects and are associated with better compliance than estrogen therapy, but little is known about the pharmacological mechanisms of such treatments. This study aimed to explore the mechanisms responsible for the estrogen-like effects of five widely prescribed Japanese herbal medicines (unkeito, kamishoyosan, nyoshinsan, keishibukuryogan, and tokishakuyakusan). We evaluated the estrogenic activity of these five Japanese herbal medicines and their metabolites using an estrogen receptor (ER)-dependent cell proliferation bioassay and an ER-dependent reporter assay. We also investigated the estrogenic activity of the crude drugs within the medicines and attempted to detect inter-crude drug synergistic effects using the ER-dependent reporter assay. We found that unkeito, kamishoyosan, and nyoshinsan exhibited estrogenic activity, and they displayed stronger estrogenic activity after being metabolized. Then, we focused on investigating the estrogenic activity of the crude drugs present within unkeito. We found that Glycyrrhizae Radix, cinnamomi cortex, evodiae fructus, and zingiberis rhizoma demonstrated ERβ-dependent estrogenic activity. The combined use of evodiae fructus and Glycyrrhizae Radix, or evodiae fructus and cinnamomi cortex produced synergistic ERβ-dependent estrogenic activity. It was suggested that unkeito, kamishoyosan, and nyoshinsan exert estrogenic activity, and hence, might be useful for treating menopausal syndrome. Furthermore, synergistic estrogenic effects were detected between some of the crude drugs present within unkeito.
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The in vitro estrogenic activity of the crude drugs found in Japanese herbal medicines prescribed for menopausal syndrome was enhanced by combining them
BMC Complementary and Alternative Medicine, 2018Co-Authors: Zeyun Wang, Takaki Shimono, Seiji Kanda, Dambajamts Enkh-undraa, Toshimasa NishiyamaAbstract:Background Japanese herbal medicines can be used as alternatives to estrogen therapy and are sometimes prescribed for menopausal syndrome because they have fewer side effects and are associated with better compliance than estrogen therapy, but little is known about the pharmacological mechanisms of such treatments. This study aimed to explore the mechanisms responsible for the estrogen-like effects of five widely prescribed Japanese herbal medicines (unkeito , kamishoyosan , nyoshinsan , keishibukuryogan , and tokishakuyakusan ). Methods We evaluated the estrogenic activity of these five Japanese herbal medicines and their metabolites using an estrogen receptor (ER)-dependent cell proliferation bioassay and an ER-dependent reporter assay. We also investigated the estrogenic activity of the crude drugs within the medicines and attempted to detect inter-crude drug synergistic effects using the ER-dependent reporter assay. Results We found that unkeito , kamishoyosan , and nyoshinsan exhibited estrogenic activity, and they displayed stronger estrogenic activity after being metabolized. Then, we focused on investigating the estrogenic activity of the crude drugs present within unkeito . We found that Glycyrrhizae Radix, cinnamomi cortex, evodiae fructus, and zingiberis rhizoma demonstrated ERβ-dependent estrogenic activity. The combined use of evodiae fructus and Glycyrrhizae Radix, or evodiae fructus and cinnamomi cortex produced synergistic ERβ-dependent estrogenic activity. Conclusion It was suggested that unkeito , kamishoyosan , and nyoshinsan exert estrogenic activity, and hence, might be useful for treating menopausal syndrome. Furthermore, synergistic estrogenic effects were detected between some of the crude drugs present within unkeito .
Zeyun Wang - One of the best experts on this subject based on the ideXlab platform.
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the in vitro estrogenic activity of the crude drugs found in japanese herbal medicines prescribed for menopausal syndrome was enhanced by combining them
BMC Complementary and Alternative Medicine, 2018Co-Authors: Zeyun Wang, Dambajamts Enkhundraa, Takaki Shimono, Seiji Kanda, Toshimasa NishiyamaAbstract:Japanese herbal medicines can be used as alternatives to estrogen therapy and are sometimes prescribed for menopausal syndrome because they have fewer side effects and are associated with better compliance than estrogen therapy, but little is known about the pharmacological mechanisms of such treatments. This study aimed to explore the mechanisms responsible for the estrogen-like effects of five widely prescribed Japanese herbal medicines (unkeito, kamishoyosan, nyoshinsan, keishibukuryogan, and tokishakuyakusan). We evaluated the estrogenic activity of these five Japanese herbal medicines and their metabolites using an estrogen receptor (ER)-dependent cell proliferation bioassay and an ER-dependent reporter assay. We also investigated the estrogenic activity of the crude drugs within the medicines and attempted to detect inter-crude drug synergistic effects using the ER-dependent reporter assay. We found that unkeito, kamishoyosan, and nyoshinsan exhibited estrogenic activity, and they displayed stronger estrogenic activity after being metabolized. Then, we focused on investigating the estrogenic activity of the crude drugs present within unkeito. We found that Glycyrrhizae Radix, cinnamomi cortex, evodiae fructus, and zingiberis rhizoma demonstrated ERβ-dependent estrogenic activity. The combined use of evodiae fructus and Glycyrrhizae Radix, or evodiae fructus and cinnamomi cortex produced synergistic ERβ-dependent estrogenic activity. It was suggested that unkeito, kamishoyosan, and nyoshinsan exert estrogenic activity, and hence, might be useful for treating menopausal syndrome. Furthermore, synergistic estrogenic effects were detected between some of the crude drugs present within unkeito.
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The in vitro estrogenic activity of the crude drugs found in Japanese herbal medicines prescribed for menopausal syndrome was enhanced by combining them
BMC Complementary and Alternative Medicine, 2018Co-Authors: Zeyun Wang, Takaki Shimono, Seiji Kanda, Dambajamts Enkh-undraa, Toshimasa NishiyamaAbstract:Background Japanese herbal medicines can be used as alternatives to estrogen therapy and are sometimes prescribed for menopausal syndrome because they have fewer side effects and are associated with better compliance than estrogen therapy, but little is known about the pharmacological mechanisms of such treatments. This study aimed to explore the mechanisms responsible for the estrogen-like effects of five widely prescribed Japanese herbal medicines (unkeito , kamishoyosan , nyoshinsan , keishibukuryogan , and tokishakuyakusan ). Methods We evaluated the estrogenic activity of these five Japanese herbal medicines and their metabolites using an estrogen receptor (ER)-dependent cell proliferation bioassay and an ER-dependent reporter assay. We also investigated the estrogenic activity of the crude drugs within the medicines and attempted to detect inter-crude drug synergistic effects using the ER-dependent reporter assay. Results We found that unkeito , kamishoyosan , and nyoshinsan exhibited estrogenic activity, and they displayed stronger estrogenic activity after being metabolized. Then, we focused on investigating the estrogenic activity of the crude drugs present within unkeito . We found that Glycyrrhizae Radix, cinnamomi cortex, evodiae fructus, and zingiberis rhizoma demonstrated ERβ-dependent estrogenic activity. The combined use of evodiae fructus and Glycyrrhizae Radix, or evodiae fructus and cinnamomi cortex produced synergistic ERβ-dependent estrogenic activity. Conclusion It was suggested that unkeito , kamishoyosan , and nyoshinsan exert estrogenic activity, and hence, might be useful for treating menopausal syndrome. Furthermore, synergistic estrogenic effects were detected between some of the crude drugs present within unkeito .
Jin Sook Kim - One of the best experts on this subject based on the ideXlab platform.
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anti angiogenic effect of eghb010 a standardized herbal formula of paeoniae Radix and Glycyrrhizae Radix
Pakistan Journal of Pharmaceutical Sciences, 2020Co-Authors: Eunsoo Jung, Chan-sik Kim, Jin Sook Kim, Wookwon Jung, Subin Park, Jung Hyun KimAbstract:EGHB010 is a standardized herbal formula of the rhizome mixture of Paeonia lactiflora Pallas and Glycyrrhiza uralensis Fisch. Neovascularization in the retina is a common pathophysiology of diabetic retinal microvasculopathy and exudative macular degeneration. In this study, we evaluated the inhibitory effects of EGHB010 on abnormal retinal angiogenesis in a hyperoxia-induced neovascular retinopathy model. Vascular endothelial growth factor (VEGF)-mediated vascular tube formation was assayed in human umbilical vascular endothelial cells (HUVECs). Experimental angiogenesis in the retinas was induced by exposing C57BL/6 pups to hyperoxic environment (75% oxygen) on postnatal day 7 (P7) and then returning them to normal oxygen pressure on P12. EGHB010 (50 and 100 mg/kg/day) was administered intraperitoneally for 5 days (P12 - P16). Retinal flat mounts were prepared to measure the extent of retinal neovascularization on P17. The incubation of HUVECs with EGHB010 (1-25 μg/mL) resulted in the inhibition of VEGF-mediated tube formation in a dose-dependent manner. EGHB010 at doses of 50 and 100 mg/kg/day inhibited the formation of retinal neovascular tufts by 31.15±2.28% and 59.83±2.92%, respectively. Together, our results indicate that EGHB010 is a potent anti-angiogenic agent and may have potential for the control of abnormal retinal vessel growth in patients with ischemic retinopathy.
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KIOM-79 protects AGE-induced retinal pericyte apoptosis via inhibition of NF-kappaB activation
2016Co-Authors: Jung Hyun Kim, Eunjin Sohn, Chan-sik Kim, Yun Mi Lee, Jin Sook KimAbstract:KIOM-79 is an herbal mixture of parched Puerariae Radix, gingered Magnoliae cortex, Glycyrrhizae Radix and Euphorbiae Radix. In the present study, we determined the efficacy and possible mechanism of KIOM-79 on the advanced glycation end product (AGE)-modified bovine serum albumin (BSA)-induced apoptosis of cultured bovine retinal pericytes and rat retinal pericytes in Zucker diabetic fatty (ZDF) rats. Seven-week-old male ZDF rats were treated with KIOM-79 (50 mg/kg body weight) once a day orally for 13 weeks. KIOM-79 significantly inhibited pericyte apoptosis which were induced by the AGE-BSA treatment. The KIOM-79 treatment markedly suppressed the activation of nuclear factor-kappaB (NF-kB) through the inhibition of inhibitory kB kinase complex. In addition, the oral administration of KIOM-79 inhibited the changes in retinal vasculature (vascular hyperpermeability, acellular capillary). KIOM-79 strongly inhibited pericyte apoptosis, NF-kB activation and the expression of pro-apoptotic Bax and tumor necrosis factor-a. Our results suggest that KIOM-79 may exert inhibitory effects on AGE-induced pericyte apoptosis by blocking NF-kB activation, thereby ameliorating retinal microvascular dysfunction
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Research Article Effect of KIOM-79 on Diabetes-Induced Myocardial Fibrosis in Zucker Diabetic Fatty Rats
2015Co-Authors: Jung Hyun Kim, Eunjin Sohn, Chan-sik Kim, Yun Mi Lee, Jin Sook KimAbstract:which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. KIOM-79, a herbal mixture of parched Puerariae Radix, gingered Magnoliae cortex, Glycyrrhizae Radix, and Euphorbiae Radix, has a strong inhibitory effect on advanced glycation end products (AGEs) formation. We investigated the beneficial effects of KIOM-79 on cardiac fibrosis in Zucker diabetic fatty (ZDF) rats. KIOM-79 (50 or 500mg/kg/day) was orally administered for 13 weeks. AGEs formation and collagen expression in the myocardium were assessed by immunohistochemistry. The expression levels of the receptor for AGEs (RAGE), transforming growth factor
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Effect of KIOM-79 on Diabetes-Induced Myocardial Fibrosis in Zucker Diabetic Fatty Rats
Evidence-based complementary and alternative medicine : eCAM, 2013Co-Authors: Jung Hyun Kim, Eunjin Sohn, Chan-sik Kim, Yun Mi Lee, Jin Sook KimAbstract:KIOM-79, a herbal mixture of parched Puerariae Radix, gingered Magnoliae cortex, Glycyrrhizae Radix, and Euphorbiae Radix, has a strong inhibitory effect on advanced glycation end products (AGEs) formation. We investigated the beneficial effects of KIOM-79 on cardiac fibrosis in Zucker diabetic fatty (ZDF) rats. KIOM-79 (50 or 500 mg/kg/day) was orally administered for 13 weeks. AGEs formation and collagen expression in the myocardium were assessed by immunohistochemistry. The expression levels of the receptor for AGEs (RAGE), transforming growth factor-β1 (TGF-β1), collagen IV, fibronectin, urotensin II, and urotensin II receptor were examined in the myocardial tissue of ZDF rats. KIOM-79 treatment at 500 mg/kg inhibited the accumulation of AGEs, reduced RAGE mRNA and protein expression, and reduced the upregulation of cardiac fibrogenic factors, such as fibronectin and collagen IV, in heart of ZDF rats. Additionally, KIOM-79 ameliorated urotensin II/receptor gene expression in the cardiac tissue of ZDF rats. Our findings indicate that KIOM-79 diminishes cardiac fibrosis in ZDF rats by preventing AGEs accumulation and RAGE overexpression and by modulating the cardiac urotensin II/receptor pathway, which decreases the amount of profibrotic factors, such as TGF-β1, fibronectin, and collagen in cardiac tissue.
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Copyright � The Korean Academy of Medical Sciences Effects of Mixed Herbal Extracts from Parched Puerariae Radix, Gingered Magnoliae Cortex, Glycyrrhizae Radix and Euphorbiae Radix (KIOM-79) on Cardiac Ion Channels and Action Potentials
2013Co-Authors: Su Jung Park, Jin Sook Kim, Kwan Seok Choi, Dong Hoon Shin, Dae Sik Jang, Jae Beom Youm, Han Choe, Yung E Earm, Sung Joon KimAbstract:KIOM-79, a mixture of ethanol extracts from four herbs (parched Puerariae Radix, gingered Magnoliae cortex, Glycyrrhizae Radix and Euphorbiae Radix), has been developed for the potential therapeutic application to diabetic symptoms. Because screening of unexpected cardiac arrhythmia is compulsory for the new drug development, we investigated the effects of KIOM-79 on the action potential (AP) and various ion channel currents in cardiac myocytes. KIOM-79 decreased the upstroke velocity (Vmax) and plateau potential while slightly increased the duration of action potential (APD). Consistent with the decreased Vmax and plateau potential, the peak amplitude of Na + current (INa) and Ca 2+ current (ICa,L) were decreased by KIOM-79. KIOM-79 showed dual effects on hERG K + current; increase of depolarization phase current (Idepol) and decreased tail current at repolarization phase (Itail). The increase of APD was suspected due to the decreased Itail. In computer simulation, the change of cardiac action potential could be well simulated based on the effects of KIOM-79 on various membrane currents. As a whole, the influence of KIOM-79 on cardiac ion channels are minor at concentrations effective for the diabetic models (0.1-10 μg
Takaki Shimono - One of the best experts on this subject based on the ideXlab platform.
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the in vitro estrogenic activity of the crude drugs found in japanese herbal medicines prescribed for menopausal syndrome was enhanced by combining them
BMC Complementary and Alternative Medicine, 2018Co-Authors: Zeyun Wang, Dambajamts Enkhundraa, Takaki Shimono, Seiji Kanda, Toshimasa NishiyamaAbstract:Japanese herbal medicines can be used as alternatives to estrogen therapy and are sometimes prescribed for menopausal syndrome because they have fewer side effects and are associated with better compliance than estrogen therapy, but little is known about the pharmacological mechanisms of such treatments. This study aimed to explore the mechanisms responsible for the estrogen-like effects of five widely prescribed Japanese herbal medicines (unkeito, kamishoyosan, nyoshinsan, keishibukuryogan, and tokishakuyakusan). We evaluated the estrogenic activity of these five Japanese herbal medicines and their metabolites using an estrogen receptor (ER)-dependent cell proliferation bioassay and an ER-dependent reporter assay. We also investigated the estrogenic activity of the crude drugs within the medicines and attempted to detect inter-crude drug synergistic effects using the ER-dependent reporter assay. We found that unkeito, kamishoyosan, and nyoshinsan exhibited estrogenic activity, and they displayed stronger estrogenic activity after being metabolized. Then, we focused on investigating the estrogenic activity of the crude drugs present within unkeito. We found that Glycyrrhizae Radix, cinnamomi cortex, evodiae fructus, and zingiberis rhizoma demonstrated ERβ-dependent estrogenic activity. The combined use of evodiae fructus and Glycyrrhizae Radix, or evodiae fructus and cinnamomi cortex produced synergistic ERβ-dependent estrogenic activity. It was suggested that unkeito, kamishoyosan, and nyoshinsan exert estrogenic activity, and hence, might be useful for treating menopausal syndrome. Furthermore, synergistic estrogenic effects were detected between some of the crude drugs present within unkeito.
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The in vitro estrogenic activity of the crude drugs found in Japanese herbal medicines prescribed for menopausal syndrome was enhanced by combining them
BMC Complementary and Alternative Medicine, 2018Co-Authors: Zeyun Wang, Takaki Shimono, Seiji Kanda, Dambajamts Enkh-undraa, Toshimasa NishiyamaAbstract:Background Japanese herbal medicines can be used as alternatives to estrogen therapy and are sometimes prescribed for menopausal syndrome because they have fewer side effects and are associated with better compliance than estrogen therapy, but little is known about the pharmacological mechanisms of such treatments. This study aimed to explore the mechanisms responsible for the estrogen-like effects of five widely prescribed Japanese herbal medicines (unkeito , kamishoyosan , nyoshinsan , keishibukuryogan , and tokishakuyakusan ). Methods We evaluated the estrogenic activity of these five Japanese herbal medicines and their metabolites using an estrogen receptor (ER)-dependent cell proliferation bioassay and an ER-dependent reporter assay. We also investigated the estrogenic activity of the crude drugs within the medicines and attempted to detect inter-crude drug synergistic effects using the ER-dependent reporter assay. Results We found that unkeito , kamishoyosan , and nyoshinsan exhibited estrogenic activity, and they displayed stronger estrogenic activity after being metabolized. Then, we focused on investigating the estrogenic activity of the crude drugs present within unkeito . We found that Glycyrrhizae Radix, cinnamomi cortex, evodiae fructus, and zingiberis rhizoma demonstrated ERβ-dependent estrogenic activity. The combined use of evodiae fructus and Glycyrrhizae Radix, or evodiae fructus and cinnamomi cortex produced synergistic ERβ-dependent estrogenic activity. Conclusion It was suggested that unkeito , kamishoyosan , and nyoshinsan exert estrogenic activity, and hence, might be useful for treating menopausal syndrome. Furthermore, synergistic estrogenic effects were detected between some of the crude drugs present within unkeito .
Seiji Kanda - One of the best experts on this subject based on the ideXlab platform.
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the in vitro estrogenic activity of the crude drugs found in japanese herbal medicines prescribed for menopausal syndrome was enhanced by combining them
BMC Complementary and Alternative Medicine, 2018Co-Authors: Zeyun Wang, Dambajamts Enkhundraa, Takaki Shimono, Seiji Kanda, Toshimasa NishiyamaAbstract:Japanese herbal medicines can be used as alternatives to estrogen therapy and are sometimes prescribed for menopausal syndrome because they have fewer side effects and are associated with better compliance than estrogen therapy, but little is known about the pharmacological mechanisms of such treatments. This study aimed to explore the mechanisms responsible for the estrogen-like effects of five widely prescribed Japanese herbal medicines (unkeito, kamishoyosan, nyoshinsan, keishibukuryogan, and tokishakuyakusan). We evaluated the estrogenic activity of these five Japanese herbal medicines and their metabolites using an estrogen receptor (ER)-dependent cell proliferation bioassay and an ER-dependent reporter assay. We also investigated the estrogenic activity of the crude drugs within the medicines and attempted to detect inter-crude drug synergistic effects using the ER-dependent reporter assay. We found that unkeito, kamishoyosan, and nyoshinsan exhibited estrogenic activity, and they displayed stronger estrogenic activity after being metabolized. Then, we focused on investigating the estrogenic activity of the crude drugs present within unkeito. We found that Glycyrrhizae Radix, cinnamomi cortex, evodiae fructus, and zingiberis rhizoma demonstrated ERβ-dependent estrogenic activity. The combined use of evodiae fructus and Glycyrrhizae Radix, or evodiae fructus and cinnamomi cortex produced synergistic ERβ-dependent estrogenic activity. It was suggested that unkeito, kamishoyosan, and nyoshinsan exert estrogenic activity, and hence, might be useful for treating menopausal syndrome. Furthermore, synergistic estrogenic effects were detected between some of the crude drugs present within unkeito.
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The in vitro estrogenic activity of the crude drugs found in Japanese herbal medicines prescribed for menopausal syndrome was enhanced by combining them
BMC Complementary and Alternative Medicine, 2018Co-Authors: Zeyun Wang, Takaki Shimono, Seiji Kanda, Dambajamts Enkh-undraa, Toshimasa NishiyamaAbstract:Background Japanese herbal medicines can be used as alternatives to estrogen therapy and are sometimes prescribed for menopausal syndrome because they have fewer side effects and are associated with better compliance than estrogen therapy, but little is known about the pharmacological mechanisms of such treatments. This study aimed to explore the mechanisms responsible for the estrogen-like effects of five widely prescribed Japanese herbal medicines (unkeito , kamishoyosan , nyoshinsan , keishibukuryogan , and tokishakuyakusan ). Methods We evaluated the estrogenic activity of these five Japanese herbal medicines and their metabolites using an estrogen receptor (ER)-dependent cell proliferation bioassay and an ER-dependent reporter assay. We also investigated the estrogenic activity of the crude drugs within the medicines and attempted to detect inter-crude drug synergistic effects using the ER-dependent reporter assay. Results We found that unkeito , kamishoyosan , and nyoshinsan exhibited estrogenic activity, and they displayed stronger estrogenic activity after being metabolized. Then, we focused on investigating the estrogenic activity of the crude drugs present within unkeito . We found that Glycyrrhizae Radix, cinnamomi cortex, evodiae fructus, and zingiberis rhizoma demonstrated ERβ-dependent estrogenic activity. The combined use of evodiae fructus and Glycyrrhizae Radix, or evodiae fructus and cinnamomi cortex produced synergistic ERβ-dependent estrogenic activity. Conclusion It was suggested that unkeito , kamishoyosan , and nyoshinsan exert estrogenic activity, and hence, might be useful for treating menopausal syndrome. Furthermore, synergistic estrogenic effects were detected between some of the crude drugs present within unkeito .