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Ali Heidarianpour - One of the best experts on this subject based on the ideXlab platform.
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Endurance exercise training restores diabetes-induced alteration in circulating Glycosylphosphatidylinositol-specific phospholipase D levels in rats
Diabetology & Metabolic Syndrome, 2020Co-Authors: Farzad Abdolmaleki, Ali HeidarianpourAbstract:Glycosylphosphatidylinositol-specific phospholipase D (GPLD1) is responsible for cleaving membrane-associated glycosylphosphatidylinositol (GPI) molecules, which is affected by diabetes. We aimed to examine the effect of 14 weeks treadmill running on serum GPLD1 levels and its association with glycemic indexes and serum Glypican-4 (GPC-4), a novel GPI-anchored adipokine, in streptozotocin-nicotinamide-induced diabetic rats. Thirty-six male Wister rats were randomly divided into three groups of twelve animals each, involving sedentary control (SC), sedentary diabetic (SD), and trained diabetic (TD) groups. The diabetes was induced through intraperitoneal injection of 120 mg/kg nicotinamide 15 min prior to intraperitoneal injection of 65 mg/kg streptozotocin in SD and TD groups. The TD group was exercised on a treadmill for 60 min/days, 5 days/wk at 26 m/min, and zero grade for 14 weeks. Following the experiment period, blood samples were taken from all animals and analyzed for experimental indexes via sandwich ELISA. Exercise training caused a significant decrease in the elevated blood glucose levels and a significant increase in the lowered blood insulin levels in TD rats (both p
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Endurance exercise training restores diabetes-induced alteration in circulating Glycosylphosphatidylinositol-specific phospholipase D levels in rats
Diabetology & Metabolic Syndrome, 2020Co-Authors: Farzad Abdolmaleki, Ali HeidarianpourAbstract:Background Glycosylphosphatidylinositol-specific phospholipase D (GPLD1) is responsible for cleaving membrane-associated glycosylphosphatidylinositol (GPI) molecules, which is affected by diabetes. We aimed to examine the effect of 14 weeks treadmill running on serum GPLD1 levels and its association with glycemic indexes and serum Glypican-4 (GPC-4), a novel GPI-anchored adipokine, in streptozotocin-nicotinamide-induced diabetic rats. Methods Thirty-six male Wister rats were randomly divided into three groups of twelve animals each, involving sedentary control (SC), sedentary diabetic (SD), and trained diabetic (TD) groups. The diabetes was induced through intraperitoneal injection of 120 mg/kg nicotinamide 15 min prior to intraperitoneal injection of 65 mg/kg streptozotocin in SD and TD groups. The TD group was exercised on a treadmill for 60 min/days, 5 days/wk at 26 m/min, and zero grade for 14 weeks. Following the experiment period, blood samples were taken from all animals and analyzed for experimental indexes via sandwich ELISA. Results Exercise training caused a significant decrease in the elevated blood glucose levels and a significant increase in the lowered blood insulin levels in TD rats (both p
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The response of serum Glypican-4 levels and its potential regulatory mechanism to endurance training and chamomile flowers’ hydroethanolic extract in streptozotocin–nicotinamide-induced diabetic rats
Acta Diabetologica, 2018Co-Authors: Farzad Abdolmaleki, Ali HeidarianpourAbstract:Aims Glypican-4 (GPC-4) is a novel adipomyokine that enhances insulin signaling. Glycosylphosphatidylinositol-specific phospholipase D (GPLD1) is thought to release GPC-4 and is itself an insulin-regulated enzyme. Beneficial effects of exercise training and chamomile flowers extract (CFE) are shown through activation of PPARγ, which is a promising drug target in diabetes and associated with GPC-4 synthesis. This study investigated the effects of 14-week treadmill running and CFE on serum GPC-4, GPLD1, and insulin levels in streptozotocin–nicotinamide (STZ–NA)-induced diabetic rats. Methods Thirty-two STZ–NA-induced diabetic male Wistar rats were randomly assigned to four groups: control (C), training (T), CFE treatment (CFE), and training plus CFE treatment (TCFE) groups. The training groups were exercised on treadmill 5 days/week and the treating groups were fed with 200 mg/kg/day CFE in drinking water for 14 weeks. Finally, serum GPC-4, GPLD1, and insulin levels were analyzed via sandwich ELISA. Results Compared to the control group, serum insulin levels were significantly higher in the T, CFE, and TCFE groups ( p
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the response of serum Glypican 4 levels and its potential regulatory mechanism to endurance training and chamomile flowers hydroethanolic extract in streptozotocin nicotinamide induced diabetic rats
Acta Diabetologica, 2018Co-Authors: Farzad Abdolmaleki, Ali HeidarianpourAbstract:Aims Glypican-4 (GPC-4) is a novel adipomyokine that enhances insulin signaling. Glycosylphosphatidylinositol-specific phospholipase D (GPLD1) is thought to release GPC-4 and is itself an insulin-regulated enzyme. Beneficial effects of exercise training and chamomile flowers extract (CFE) are shown through activation of PPARγ, which is a promising drug target in diabetes and associated with GPC-4 synthesis. This study investigated the effects of 14-week treadmill running and CFE on serum GPC-4, GPLD1, and insulin levels in streptozotocin–nicotinamide (STZ–NA)-induced diabetic rats.
Farzad Abdolmaleki - One of the best experts on this subject based on the ideXlab platform.
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Endurance exercise training restores diabetes-induced alteration in circulating Glycosylphosphatidylinositol-specific phospholipase D levels in rats
Diabetology & Metabolic Syndrome, 2020Co-Authors: Farzad Abdolmaleki, Ali HeidarianpourAbstract:Glycosylphosphatidylinositol-specific phospholipase D (GPLD1) is responsible for cleaving membrane-associated glycosylphosphatidylinositol (GPI) molecules, which is affected by diabetes. We aimed to examine the effect of 14 weeks treadmill running on serum GPLD1 levels and its association with glycemic indexes and serum Glypican-4 (GPC-4), a novel GPI-anchored adipokine, in streptozotocin-nicotinamide-induced diabetic rats. Thirty-six male Wister rats were randomly divided into three groups of twelve animals each, involving sedentary control (SC), sedentary diabetic (SD), and trained diabetic (TD) groups. The diabetes was induced through intraperitoneal injection of 120 mg/kg nicotinamide 15 min prior to intraperitoneal injection of 65 mg/kg streptozotocin in SD and TD groups. The TD group was exercised on a treadmill for 60 min/days, 5 days/wk at 26 m/min, and zero grade for 14 weeks. Following the experiment period, blood samples were taken from all animals and analyzed for experimental indexes via sandwich ELISA. Exercise training caused a significant decrease in the elevated blood glucose levels and a significant increase in the lowered blood insulin levels in TD rats (both p
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Endurance exercise training restores diabetes-induced alteration in circulating Glycosylphosphatidylinositol-specific phospholipase D levels in rats
Diabetology & Metabolic Syndrome, 2020Co-Authors: Farzad Abdolmaleki, Ali HeidarianpourAbstract:Background Glycosylphosphatidylinositol-specific phospholipase D (GPLD1) is responsible for cleaving membrane-associated glycosylphosphatidylinositol (GPI) molecules, which is affected by diabetes. We aimed to examine the effect of 14 weeks treadmill running on serum GPLD1 levels and its association with glycemic indexes and serum Glypican-4 (GPC-4), a novel GPI-anchored adipokine, in streptozotocin-nicotinamide-induced diabetic rats. Methods Thirty-six male Wister rats were randomly divided into three groups of twelve animals each, involving sedentary control (SC), sedentary diabetic (SD), and trained diabetic (TD) groups. The diabetes was induced through intraperitoneal injection of 120 mg/kg nicotinamide 15 min prior to intraperitoneal injection of 65 mg/kg streptozotocin in SD and TD groups. The TD group was exercised on a treadmill for 60 min/days, 5 days/wk at 26 m/min, and zero grade for 14 weeks. Following the experiment period, blood samples were taken from all animals and analyzed for experimental indexes via sandwich ELISA. Results Exercise training caused a significant decrease in the elevated blood glucose levels and a significant increase in the lowered blood insulin levels in TD rats (both p
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The response of serum Glypican-4 levels and its potential regulatory mechanism to endurance training and chamomile flowers’ hydroethanolic extract in streptozotocin–nicotinamide-induced diabetic rats
Acta Diabetologica, 2018Co-Authors: Farzad Abdolmaleki, Ali HeidarianpourAbstract:Aims Glypican-4 (GPC-4) is a novel adipomyokine that enhances insulin signaling. Glycosylphosphatidylinositol-specific phospholipase D (GPLD1) is thought to release GPC-4 and is itself an insulin-regulated enzyme. Beneficial effects of exercise training and chamomile flowers extract (CFE) are shown through activation of PPARγ, which is a promising drug target in diabetes and associated with GPC-4 synthesis. This study investigated the effects of 14-week treadmill running and CFE on serum GPC-4, GPLD1, and insulin levels in streptozotocin–nicotinamide (STZ–NA)-induced diabetic rats. Methods Thirty-two STZ–NA-induced diabetic male Wistar rats were randomly assigned to four groups: control (C), training (T), CFE treatment (CFE), and training plus CFE treatment (TCFE) groups. The training groups were exercised on treadmill 5 days/week and the treating groups were fed with 200 mg/kg/day CFE in drinking water for 14 weeks. Finally, serum GPC-4, GPLD1, and insulin levels were analyzed via sandwich ELISA. Results Compared to the control group, serum insulin levels were significantly higher in the T, CFE, and TCFE groups ( p
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the response of serum Glypican 4 levels and its potential regulatory mechanism to endurance training and chamomile flowers hydroethanolic extract in streptozotocin nicotinamide induced diabetic rats
Acta Diabetologica, 2018Co-Authors: Farzad Abdolmaleki, Ali HeidarianpourAbstract:Aims Glypican-4 (GPC-4) is a novel adipomyokine that enhances insulin signaling. Glycosylphosphatidylinositol-specific phospholipase D (GPLD1) is thought to release GPC-4 and is itself an insulin-regulated enzyme. Beneficial effects of exercise training and chamomile flowers extract (CFE) are shown through activation of PPARγ, which is a promising drug target in diabetes and associated with GPC-4 synthesis. This study investigated the effects of 14-week treadmill running and CFE on serum GPC-4, GPLD1, and insulin levels in streptozotocin–nicotinamide (STZ–NA)-induced diabetic rats.
Pablo Lapunzina - One of the best experts on this subject based on the ideXlab platform.
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Simpson-Golabi-Behmel syndrome types I and II
Orphanet Journal of Rare Diseases, 2014Co-Authors: Jair Tenorio, Pedro Arias, Víctor Martínez-glez, Fernando Santos, Sixto García-miñaur, Julián Nevado, Pablo LapunzinaAbstract:El Síndrome de Simpson-Golabi-Behmel (SSGB) es un Síndrome de sobrecrecimiento raro, que se caracteriza clínicamente por múltiples anomalías congénitas, sobrecrecimiento pre y post natal, rasgos craneofaciales distintivos, macrocefalia y organomegalia. Otras características que pueden presentar estos pacientes incluyen anomalías en el aparato esquelético, el corazón, el sistema nervioso central, el riñón y el tracto gastrointestinal. También pueden presentar discapacidad intelectual, retraso motor precoz y retraso en el habla, aunque en su mayoría, estos individuos presentan una inteligencia dentro de los límites normales. Los reordenamientos genómicos y las mutaciones puntuales que incluyen el gen GPC3 (“Glypican-3 gene”) localizado en la región cromosómica Xq26, se han asociado con la aparición del SSGB. Ocasionalmente, estos reordenamientos genómicos pueden incluir el gen GPC4 . Los glipicanos son proteoglicanos de heparán sulfato que actúan controlando el crecimiento y división celular. Aunque se ha descrito una forma letal de este Síndrome (denominada SSGB tipo II) en esta revisión sólo analizamos la forma clásica de este Síndrome y sólo comentaremos algunos aspectos del SSGB tipo II. En este trabajo se presenta una revisión de todos los aspectos clínicos y moleculares de este Síndrome, actualizando algunos aspectos y además se sugiere un esquema de seguimiento de estos pacientes por parte de genetistas y médicos de atención primaria. Simpson-Golabi-Behmel syndrome (SGBS) is a rare overgrowth syndrome clinically characterized by multiple congenital abnormalities, pre/postnatal overgrowth, distinctive craniofacial features, macrocephaly, and organomegaly. Abnormalities of the skeletal system, heart, central nervous system, kidney, and gastrointestinal tract may also be observed. Intellectual disability, early motor milestones and speech delay are sometimes present; however, there are a considerable number of individuals with normal intelligence. Genomic rearrangements and point mutations involving the Glypican-3 gene ( GPC3 ) at Xq26 have been shown to be associated with SGBS. Occasionally, these rearrangements also include the Glypican-4 gene ( GPC4 ). Glypicans are heparan sulfate proteoglycans which have a role in the control of cell growth and cell division. Although a lethal and infrequent form (also known as SGBS type II) has been described, only the classical form of SGBS is reviewed in this work, whereas only some specific features on SGBS type II are commented. We review all clinical and molecular aspects of this rare disorder, updating many topics and suggest a follow-up scheme for geneticists and primary care clinicians.
Sang Ouk Chin - One of the best experts on this subject based on the ideXlab platform.
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correlation of Glypican 4 level with basal active glucagon like peptide 1 level in patients with type 2 diabetes mellitus
Endocrinology and Metabolism, 2016Co-Authors: Sang Ouk ChinAbstract:BACKGROUND: Previous studies have reported that Glypican-4 (GPC4) regulates insulin signaling by interacting with insulin receptor and through adipocyte differentiation. However, GPC4 has not been studied with regard to its effects on clinical factors in patients with type 2 diabetes mellitus (T2DM). We aimed to identify factors associated with GPC4 level in T2DM. METHODS: Between January 2010 and December 2013, we selected 152 subjects with T2DM and collected serum and plasma into tubes pretreated with aprotinin and dipeptidyl peptidase-4 inhibitor to preserve active gastric inhibitory polypeptide (GIP) and glucagon-like peptide 1 (GLP-1). GPC4, active GLP-1, active GIP, and other factors were measured in these plasma samples. We performed a linear regression analysis to identify factors associated with GPC4 level. RESULTS: The subjects had a mean age of 58.1 years, were mildly obese (mean body mass index [BMI], 26.1 kg/m²), had T2DM of long-duration (mean, 101.3 months), glycated hemoglobin 7.5%, low insulin secretion, and low insulin resistance (mean homeostatic model assessment of insulin resistance [HOMA-IR], 1.2). Their mean GPC4 was 2.0±0.2 ng/mL. In multivariate analysis, GPC4 was independently associated with age (β=0.224, P=0.009), and levels of active GLP-1 (β=0.171, P=0.049) and aspartate aminotransferase (AST; β=-0.176, P=0.043) after being adjusted for other clinical factors. CONCLUSION: GPC4 was independently associated with age, active GLP-1, and AST in T2DM patients, but was not associated with HOMA-IR and BMI, which are well known factors related to GPC4. Further study is needed to identify the mechanisms of the association between GPC4 and basal active GLP-1 levels.
Wilhelm Ansorge - One of the best experts on this subject based on the ideXlab platform.
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comparative characteristics of mesenchymal stem cells from human bone marrow adipose tissue and umbilical cord blood
Experimental Hematology, 2005Co-Authors: Wolfgang Wagner, Frederik Wein, Anja Seckinger, Maria Frankhauser, Ute Wirkner, Ulf Krause, Jonathon Blake, Christian Schwager, Volker Eckstein, Wilhelm AnsorgeAbstract:Objective Various preparative protocols have been proposed for the acquisition and cultivation of mesenchymal stem cells (MSC). Whereas surface antigen markers have failed to precisely define this population, microarray analysis might provide a better tool for characterization of MSC. Methods In this study, we have analyzed global gene expression profiles of human MSC isolated from adipose tissue (AT), from umbilical cord blood (CB), and from bone marrow (BM) under two growth conditions and have compared them to terminally differentiated human fibroblasts (HS68). Profiles were compared using our Human Genome Microarray representing 51.144 different cDNA clones. Results Cultured with the appropriate conditions, osteogenic and adipogenic differentiation could be confirmed in all MSC preparations but not in fibroblasts. No phenotypic differences were observed by flow cytometry using a panel of 22 surface antigen markers. Whereas MSC derived from different donors using the same culture procedure yielded a consistent and reproducible gene expression profile, many genes were differentially expressed in MSC from different ontogenetic sources or from different culture conditions. Twenty-five genes were overlapping and upregulated in all MSC preparations from AT, CB, and BM as compared to HS68 fibroblasts. These genes included fibronectin , ECM2 , Glypican-4 , ID1 , NF1B , HOXA5 , and HOXB6 . Many genes upregulated in MSC are involved in extracellular matrix, morphogenesis, and development, whereas several inhibitors of the Wnt pathway (DKK1 , DKK3 , SFRP1) were highly expressed in fibroblasts. Conclusion Our results have provided a foundation for a more reproducible and reliable quality control using genotypic analysis for defining MSC.