The Experts below are selected from a list of 6312 Experts worldwide ranked by ideXlab platform
Massimiliano Beltramo - One of the best experts on this subject based on the ideXlab platform.
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The kisspeptin analog C6 is a possible alternative to PMSG (pregnant mare serum gonadotropin) for triggering synchronized and fertile ovulations in the Alpine Goat
PLoS ONE, 2019Co-Authors: Caroline Decourt, Vincent Robert, Didier Lomet, Karine Anger, Marion Georgelin, Kévin Poissenot, Maria-teresa Pellicer-rubio, Vincent Aucagne, Massimiliano BeltramoAbstract:In temperate regions Goat's reproduction is seasonal. To obtain year-round Breeding, hormonal treatments are currently applied. These treatments usually combine a progesterone analog with the pregnant mare serum gonadotropin (PMSG). However, their use has significant ethical and environmental drawbacks. Therefore, alternative methods to manage reproduction are needed. The discovery that in mammals the neuropeptide kisspeptin is a major positive regulator of hypothalamo-pituitary gonadal axis offered an attractive alternative strategy to control reproduction. We have previously designed a kisspeptin analog, called C6, which offers pharmacological advantages over endogenous kisspeptin. These include a longer lasting effect and enhanced activity following intramuscular injection. In the present work, we evaluated C6 effect on LH and FSH plasma concentrations in the Alpine Goat Breed and tested whether C6 could replace PMSG to trigger ovulation. An intramuscular injection of C6 (15 nmol/doe) given 24 hours after the end of progestogen treatment induced a surge-like peak of both LH and FSH. This was followed by an increase of progesterone, a hallmark of ovulation induction and corpus luteus formation. These results were obtained at three different time of the year: during the Breeding season, the non-Breeding season and at the onset of the Breeding season. Furthermore, we compared the efficacy of C6 and PMSG to induce fertile ovulations when these treatments are given at the onset of the Breeding season and are followed by artificial insemination. The results of this first attempt were extremely promising with gestation rates of 45% and 64% for C6 and PMSG respectively. Pending optimization of the treatment procedure in order to improve efficacy, kisspeptin analogs could be the long sought-after alternative to PMSG.
Kangle Qin - One of the best experts on this subject based on the ideXlab platform.
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a screen for key genes and pathways involved in high quality brush hair in the yangtze river delta white Goat
PLOS ONE, 2017Co-Authors: Haiyan Guo, Guohu Cheng, Hao Zhang, Kangle QinAbstract:The Yangtze River Delta White Goat is the only Goat Breed that produces high-quality brush hair, or type III hair, which is specialized for use in top-grade writing brushes. There has been little research, especially molecular research, on the traits that result in high-quality brush hair in the Yangtze River Delta White Goat. To explore the molecular mechanisms of the formation of high-quality brush hair, High-throughput RNA-Seq technology was used to compare skin samples from Yangtze River Delta White Goats that produce high-quality hair and non high-quality hair for identification of the important genes and related pathways that might influence the hair quality traits. The results showed that 295 genes were expressed differentially between the Goats with higher and lower hair quality, respectively. Of those genes, 132 were up-regulated, 62 were down-regulated, and 101 were expressed exclusively in the Goats with high-quality brush hair. Gene Ontology and Metabolic Pathway Significant Enrichment analyses of the differentially expressed genes indicated that the MAP3K1, DUSP1, DUSP6 and the MAPK signaling pathway might play important roles in the traits important for high-quality brush hair.
Haiyan Guo - One of the best experts on this subject based on the ideXlab platform.
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methylation analysis of cmtm3 and dusp1 gene promoters in high quality brush hair in the yangtze river delta white Goat
Gene, 2018Co-Authors: Wang Qiang, Haiyan Guo, Jianfei Shi, Xiuyuan YinAbstract:The Yangtze River delta white Goat is the only Goat Breed that produces high-quality brush hair, which is specifically used in top-grade writing brushes. Previous studies have indicated that the CMTM3 and DUSP1 genes are involved in the growth and cycle of high-quality brush hair, and these genes are thought to be involved in the formation of high-quality brush hair traits. In this study, we investigated the relationship between methylation of CMTM3 and DUSP1 and such traits. The results indicated that the relative expression levels of the CMTM3 and DUSP1 genes were higher in non-high-quality brush hair than in high-quality brush hair. Furthermore, the CpG sites of the DUSP1 gene were not methylated, and the methylation level of CMTM3 was negatively correlated with the gene expression level. We believe that the DUSP1 gene regulates the formation of high-quality brush hair by non-methylated, and that methylation of the CMTM3 gene results in a decrease in its expression, causing an increase in the activity of the androgen receptor and the level of androgen. This high androgen level promotes the growth of high-quality brush hair. These study results provide a theoretical basis for further elucidating the molecular mechanism of the formation of high-quality brush hair characteristics, and provide scientific reference for the molecular Breeding of high-quality brush hair.
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a screen for key genes and pathways involved in high quality brush hair in the yangtze river delta white Goat
PLOS ONE, 2017Co-Authors: Haiyan Guo, Guohu Cheng, Hao Zhang, Kangle QinAbstract:The Yangtze River Delta White Goat is the only Goat Breed that produces high-quality brush hair, or type III hair, which is specialized for use in top-grade writing brushes. There has been little research, especially molecular research, on the traits that result in high-quality brush hair in the Yangtze River Delta White Goat. To explore the molecular mechanisms of the formation of high-quality brush hair, High-throughput RNA-Seq technology was used to compare skin samples from Yangtze River Delta White Goats that produce high-quality hair and non high-quality hair for identification of the important genes and related pathways that might influence the hair quality traits. The results showed that 295 genes were expressed differentially between the Goats with higher and lower hair quality, respectively. Of those genes, 132 were up-regulated, 62 were down-regulated, and 101 were expressed exclusively in the Goats with high-quality brush hair. Gene Ontology and Metabolic Pathway Significant Enrichment analyses of the differentially expressed genes indicated that the MAP3K1, DUSP1, DUSP6 and the MAPK signaling pathway might play important roles in the traits important for high-quality brush hair.
Caroline Decourt - One of the best experts on this subject based on the ideXlab platform.
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The kisspeptin analog C6 is a possible alternative to PMSG (pregnant mare serum gonadotropin) for triggering synchronized and fertile ovulations in the Alpine Goat
PLoS ONE, 2019Co-Authors: Caroline Decourt, Vincent Robert, Didier Lomet, Karine Anger, Marion Georgelin, Kévin Poissenot, Maria-teresa Pellicer-rubio, Vincent Aucagne, Massimiliano BeltramoAbstract:In temperate regions Goat's reproduction is seasonal. To obtain year-round Breeding, hormonal treatments are currently applied. These treatments usually combine a progesterone analog with the pregnant mare serum gonadotropin (PMSG). However, their use has significant ethical and environmental drawbacks. Therefore, alternative methods to manage reproduction are needed. The discovery that in mammals the neuropeptide kisspeptin is a major positive regulator of hypothalamo-pituitary gonadal axis offered an attractive alternative strategy to control reproduction. We have previously designed a kisspeptin analog, called C6, which offers pharmacological advantages over endogenous kisspeptin. These include a longer lasting effect and enhanced activity following intramuscular injection. In the present work, we evaluated C6 effect on LH and FSH plasma concentrations in the Alpine Goat Breed and tested whether C6 could replace PMSG to trigger ovulation. An intramuscular injection of C6 (15 nmol/doe) given 24 hours after the end of progestogen treatment induced a surge-like peak of both LH and FSH. This was followed by an increase of progesterone, a hallmark of ovulation induction and corpus luteus formation. These results were obtained at three different time of the year: during the Breeding season, the non-Breeding season and at the onset of the Breeding season. Furthermore, we compared the efficacy of C6 and PMSG to induce fertile ovulations when these treatments are given at the onset of the Breeding season and are followed by artificial insemination. The results of this first attempt were extremely promising with gestation rates of 45% and 64% for C6 and PMSG respectively. Pending optimization of the treatment procedure in order to improve efficacy, kisspeptin analogs could be the long sought-after alternative to PMSG.
Baldassare Portolano - One of the best experts on this subject based on the ideXlab platform.
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Application of microsatellite markers as potential tools for traceability of Girgentana Goat Breed dairy products
Food Research International, 2015Co-Authors: Maria Teresa Sardina, Lina Tortorici, Salvatore Mastrangelo, Rosalia Di Gerlando, Marco Tolone, Baldassare PortolanoAbstract:Abstract In livestock, Breed assignment may play a key role in the certification of products linked to specific Breeds. Traceability of farm animals and authentication of their products can contribute to improve Breed profitability and sustainability of animal productions with significant impact on the rural economy of particular geographic areas and on Breed and biodiversity conservation. With the goal of developing a Breed genetic traceability system for Girgentana dairy products, the aim of this study was to identify specific microsatellite markers able to discriminate among the most important Sicilian dairy Goat Breeds, in order to detect possible adulteration in Girgentana dairy products. A total of 20 microsatellite markers were analyzed on 338 individual samples from Girgentana, Maltese, and Derivata di Siria Goat Breeds. Specific microsatellite markers useful for traceability of dairy products were identified. Eight microsatellite markers showed alleles present at the same time in Maltese and Derivata di Siria and absent in Girgentana and, therefore, they were tested on DNA pools of the three Breeds. Considering the electropherograms' results, only FCB20, SRCRSP5, and TGLA122 markers were tested on DNA samples extracted from cheeses of Girgentana Goat Breed. These three microsatellite markers could be applied in a Breed genetic traceability system of Girgentana dairy products in order to detect adulteration due to Maltese and Derivata di Siria Goat Breeds.