The Experts below are selected from a list of 210 Experts worldwide ranked by ideXlab platform
J Kopecký - One of the best experts on this subject based on the ideXlab platform.
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Transgenic UCP1 in white adipocytes modulates mitochondrial membrane potential.
FEBS Letters, 1999Co-Authors: F Baumruk, P Flachs, M Horáková, D Floryk, J KopeckýAbstract:To test if mitochondrial uncoupling in white adipocytes is responsible for obesity resistance of the aP2-Ucp transgenic mice expressing ectopic uncoupling protein 1 (UCPI) in white Fat, mitochondrial membrane potential (delta psi(m)) was estimated by flow cytometry in adipocytes isolated from Gonadal Fat. Ectopic UCP1 (approximately 0.8 mol UCP1/mol respiratory chain) decreased the delta psi(m) and rendered the potential sensitive to GDP and Fatty acids. These ligands of UCP1 had no effect on delta psi(m) in white adipocytes from non-transgenic mice, suggesting that the function of endogenous UCP2 in adipocytes was not affected. The results support the hypothesis that mitochondrial uncoupling in white Fat may prevent development of obesity.
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Transgenic UCP1 in white adipocytes modulates mitochondrial membrane potential.
FEBS Letters, 1999Co-Authors: F Baumruk, P Flachs, M Horáková, D Floryk, J KopeckýAbstract:Abstract To test if mitochondrial uncoupling in white adipocytes is responsible for obesity resistance of the aP2-Ucp transgenic mice expressing ectopic uncoupling protein 1 (UCP1) in white Fat, mitochondrial membrane potential (ΔΨm) was estimated by flow cytometry in adipocytes isolated from Gonadal Fat. Ectopic UCP1 (approximately 0.8 mol UCP1/mol respiratory chain) decreased the ΔΨm and rendered the potential sensitive to GDP and Fatty acids. These ligands of UCP1 had no effect on ΔΨm in white adipocytes from non-transgenic mice, suggesting that the function of endogenous UCP2 in adipocytes was not affected. The results support the hypothesis that mitochondrial uncoupling in white Fat may prevent development of obesity.
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expression of the mitochondrial uncoupling protein gene from the ap2 gene promoter prevents genetic obesity
Journal of Clinical Investigation, 1995Co-Authors: J Kopecký, George Clarke, Sven Enerback, Bruce M Spiegelman, Leslie P KozakAbstract:The brown Fat-specific mitochondrial uncoupling protein (UCP) provides a mechanism for generating heat by uncoupling respiration and oxidative phosphorylation. It has been suggested that this system of thermogenesis can provide a defense against obesity. To test this idea, we created a transgenic mouse in which the Fat-specific aP2 gene promoter directed Ucp expression in white Fat and provided for the constitutive expression of Ucp in brown Fat. Transgenic mice showed both Ucp mRNA and immunoreactive UCP in white Fat at 2-10% the level normally measured in brown Fat. A reduction in subcutaneous Fat of aP2-Ucp C57BL/6J mice was observed at 3 mo of age. When the transgene was expressed in Avy genetically obese mice reductions in total body weight and subcutaneous Fat stores were observed. Female transgenic Avy mice at 13 mo of age weighed 35 grams, a weight indistinguishable from nontransgenic C57BL/6J mice. Gonadal Fat showed an increase in a novel adipocyte derivative that did not accumulate lipids and that constituted approximately 80% of the mass of the tissue in Avy transgenic. A major effect of aP2-Ucp in brown Fat was to reduce endogenous gene expression by as much as 95%. The results suggest that UCP synthesized from the aP2 gene promoter is thermogenically active and capable of reducing Fat stores.
F Baumruk - One of the best experts on this subject based on the ideXlab platform.
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Transgenic UCP1 in white adipocytes modulates mitochondrial membrane potential.
FEBS Letters, 1999Co-Authors: F Baumruk, P Flachs, M Horáková, D Floryk, J KopeckýAbstract:To test if mitochondrial uncoupling in white adipocytes is responsible for obesity resistance of the aP2-Ucp transgenic mice expressing ectopic uncoupling protein 1 (UCPI) in white Fat, mitochondrial membrane potential (delta psi(m)) was estimated by flow cytometry in adipocytes isolated from Gonadal Fat. Ectopic UCP1 (approximately 0.8 mol UCP1/mol respiratory chain) decreased the delta psi(m) and rendered the potential sensitive to GDP and Fatty acids. These ligands of UCP1 had no effect on delta psi(m) in white adipocytes from non-transgenic mice, suggesting that the function of endogenous UCP2 in adipocytes was not affected. The results support the hypothesis that mitochondrial uncoupling in white Fat may prevent development of obesity.
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Transgenic UCP1 in white adipocytes modulates mitochondrial membrane potential.
FEBS Letters, 1999Co-Authors: F Baumruk, P Flachs, M Horáková, D Floryk, J KopeckýAbstract:Abstract To test if mitochondrial uncoupling in white adipocytes is responsible for obesity resistance of the aP2-Ucp transgenic mice expressing ectopic uncoupling protein 1 (UCP1) in white Fat, mitochondrial membrane potential (ΔΨm) was estimated by flow cytometry in adipocytes isolated from Gonadal Fat. Ectopic UCP1 (approximately 0.8 mol UCP1/mol respiratory chain) decreased the ΔΨm and rendered the potential sensitive to GDP and Fatty acids. These ligands of UCP1 had no effect on ΔΨm in white adipocytes from non-transgenic mice, suggesting that the function of endogenous UCP2 in adipocytes was not affected. The results support the hypothesis that mitochondrial uncoupling in white Fat may prevent development of obesity.
M Horáková - One of the best experts on this subject based on the ideXlab platform.
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Transgenic UCP1 in white adipocytes modulates mitochondrial membrane potential.
FEBS Letters, 1999Co-Authors: F Baumruk, P Flachs, M Horáková, D Floryk, J KopeckýAbstract:To test if mitochondrial uncoupling in white adipocytes is responsible for obesity resistance of the aP2-Ucp transgenic mice expressing ectopic uncoupling protein 1 (UCPI) in white Fat, mitochondrial membrane potential (delta psi(m)) was estimated by flow cytometry in adipocytes isolated from Gonadal Fat. Ectopic UCP1 (approximately 0.8 mol UCP1/mol respiratory chain) decreased the delta psi(m) and rendered the potential sensitive to GDP and Fatty acids. These ligands of UCP1 had no effect on delta psi(m) in white adipocytes from non-transgenic mice, suggesting that the function of endogenous UCP2 in adipocytes was not affected. The results support the hypothesis that mitochondrial uncoupling in white Fat may prevent development of obesity.
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Transgenic UCP1 in white adipocytes modulates mitochondrial membrane potential.
FEBS Letters, 1999Co-Authors: F Baumruk, P Flachs, M Horáková, D Floryk, J KopeckýAbstract:Abstract To test if mitochondrial uncoupling in white adipocytes is responsible for obesity resistance of the aP2-Ucp transgenic mice expressing ectopic uncoupling protein 1 (UCP1) in white Fat, mitochondrial membrane potential (ΔΨm) was estimated by flow cytometry in adipocytes isolated from Gonadal Fat. Ectopic UCP1 (approximately 0.8 mol UCP1/mol respiratory chain) decreased the ΔΨm and rendered the potential sensitive to GDP and Fatty acids. These ligands of UCP1 had no effect on ΔΨm in white adipocytes from non-transgenic mice, suggesting that the function of endogenous UCP2 in adipocytes was not affected. The results support the hypothesis that mitochondrial uncoupling in white Fat may prevent development of obesity.
D Floryk - One of the best experts on this subject based on the ideXlab platform.
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Transgenic UCP1 in white adipocytes modulates mitochondrial membrane potential.
FEBS Letters, 1999Co-Authors: F Baumruk, P Flachs, M Horáková, D Floryk, J KopeckýAbstract:To test if mitochondrial uncoupling in white adipocytes is responsible for obesity resistance of the aP2-Ucp transgenic mice expressing ectopic uncoupling protein 1 (UCPI) in white Fat, mitochondrial membrane potential (delta psi(m)) was estimated by flow cytometry in adipocytes isolated from Gonadal Fat. Ectopic UCP1 (approximately 0.8 mol UCP1/mol respiratory chain) decreased the delta psi(m) and rendered the potential sensitive to GDP and Fatty acids. These ligands of UCP1 had no effect on delta psi(m) in white adipocytes from non-transgenic mice, suggesting that the function of endogenous UCP2 in adipocytes was not affected. The results support the hypothesis that mitochondrial uncoupling in white Fat may prevent development of obesity.
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Transgenic UCP1 in white adipocytes modulates mitochondrial membrane potential.
FEBS Letters, 1999Co-Authors: F Baumruk, P Flachs, M Horáková, D Floryk, J KopeckýAbstract:Abstract To test if mitochondrial uncoupling in white adipocytes is responsible for obesity resistance of the aP2-Ucp transgenic mice expressing ectopic uncoupling protein 1 (UCP1) in white Fat, mitochondrial membrane potential (ΔΨm) was estimated by flow cytometry in adipocytes isolated from Gonadal Fat. Ectopic UCP1 (approximately 0.8 mol UCP1/mol respiratory chain) decreased the ΔΨm and rendered the potential sensitive to GDP and Fatty acids. These ligands of UCP1 had no effect on ΔΨm in white adipocytes from non-transgenic mice, suggesting that the function of endogenous UCP2 in adipocytes was not affected. The results support the hypothesis that mitochondrial uncoupling in white Fat may prevent development of obesity.
P Flachs - One of the best experts on this subject based on the ideXlab platform.
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Transgenic UCP1 in white adipocytes modulates mitochondrial membrane potential.
FEBS Letters, 1999Co-Authors: F Baumruk, P Flachs, M Horáková, D Floryk, J KopeckýAbstract:To test if mitochondrial uncoupling in white adipocytes is responsible for obesity resistance of the aP2-Ucp transgenic mice expressing ectopic uncoupling protein 1 (UCPI) in white Fat, mitochondrial membrane potential (delta psi(m)) was estimated by flow cytometry in adipocytes isolated from Gonadal Fat. Ectopic UCP1 (approximately 0.8 mol UCP1/mol respiratory chain) decreased the delta psi(m) and rendered the potential sensitive to GDP and Fatty acids. These ligands of UCP1 had no effect on delta psi(m) in white adipocytes from non-transgenic mice, suggesting that the function of endogenous UCP2 in adipocytes was not affected. The results support the hypothesis that mitochondrial uncoupling in white Fat may prevent development of obesity.
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Transgenic UCP1 in white adipocytes modulates mitochondrial membrane potential.
FEBS Letters, 1999Co-Authors: F Baumruk, P Flachs, M Horáková, D Floryk, J KopeckýAbstract:Abstract To test if mitochondrial uncoupling in white adipocytes is responsible for obesity resistance of the aP2-Ucp transgenic mice expressing ectopic uncoupling protein 1 (UCP1) in white Fat, mitochondrial membrane potential (ΔΨm) was estimated by flow cytometry in adipocytes isolated from Gonadal Fat. Ectopic UCP1 (approximately 0.8 mol UCP1/mol respiratory chain) decreased the ΔΨm and rendered the potential sensitive to GDP and Fatty acids. These ligands of UCP1 had no effect on ΔΨm in white adipocytes from non-transgenic mice, suggesting that the function of endogenous UCP2 in adipocytes was not affected. The results support the hypothesis that mitochondrial uncoupling in white Fat may prevent development of obesity.