The Experts below are selected from a list of 222 Experts worldwide ranked by ideXlab platform
Herbert Steinbeisser - One of the best experts on this subject based on the ideXlab platform.
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Negative Autoregulation of the Organizer-specific Homeobox Gene Goosecoid
Journal of Biological Chemistry, 1998Co-Authors: Vlatko Danilov, Martin Blum, Axel Schweickert, Marina Campione, Herbert SteinbeisserAbstract:The homeobox gene Goosecoid has been implicated to play a central role in the Spemann organizer tissue of the vertebrate embryo. Misexpression of Goosecoid on the ventral side of a Xenopus laevis gastrula embryo was shown to result in a partial duplication of the primary body axis, reminiscent of the Spemann organizer graft. Normal embryonic development thus requires tight temporal and spatial control of genes instrumental for organizer function. In the present study we investigated the transcriptional control of Goosecoid gene expression. Sequence analysis of the mouse and human promoter region revealed the presence of two palindromic binding elements for homeobox genes of the prd type to which Goosecoid belongs. We show that Goosecoid Protein can bind to these sites in vitro. By using reporter gene constructs of the human and mouse promoter, we demonstrate that Goosecoid can act as a repressor of its own promoter activity in transient co-transfection experiments in mouse P19 cells and in Xenopus embryos. Autorepression depends on the presence of the homeodomain and is mediated through the prd element more proximal to the transcriptional start site. Our results suggest a role for Goosecoid in restricting organizer activity in the vertebrate gastrula embryo.
Vlatko Danilov - One of the best experts on this subject based on the ideXlab platform.
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Negative Autoregulation of the Organizer-specific Homeobox Gene Goosecoid
Journal of Biological Chemistry, 1998Co-Authors: Vlatko Danilov, Martin Blum, Axel Schweickert, Marina Campione, Herbert SteinbeisserAbstract:The homeobox gene Goosecoid has been implicated to play a central role in the Spemann organizer tissue of the vertebrate embryo. Misexpression of Goosecoid on the ventral side of a Xenopus laevis gastrula embryo was shown to result in a partial duplication of the primary body axis, reminiscent of the Spemann organizer graft. Normal embryonic development thus requires tight temporal and spatial control of genes instrumental for organizer function. In the present study we investigated the transcriptional control of Goosecoid gene expression. Sequence analysis of the mouse and human promoter region revealed the presence of two palindromic binding elements for homeobox genes of the prd type to which Goosecoid belongs. We show that Goosecoid Protein can bind to these sites in vitro. By using reporter gene constructs of the human and mouse promoter, we demonstrate that Goosecoid can act as a repressor of its own promoter activity in transient co-transfection experiments in mouse P19 cells and in Xenopus embryos. Autorepression depends on the presence of the homeodomain and is mediated through the prd element more proximal to the transcriptional start site. Our results suggest a role for Goosecoid in restricting organizer activity in the vertebrate gastrula embryo.
Martin Blum - One of the best experts on this subject based on the ideXlab platform.
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Goosecoid inhibits erythrocyte differentiation by competing with Rb for PU.1 binding in murine cells
Oncogene, 1999Co-Authors: Yuko Konishi, Martin Blum, Mari Tominaga, Yoshiya Watanabe, Fumiya Imamura, Adam Goldfarb, Richard Maki, Eddy M De Robertis, Akira TominagaAbstract:Misexpression of the dorsal mesodermal patterning factor Goosecoid on the ventral side of amphibian embryos results in inhibition of blood formation in early embryogenesis. To investigate the mechanism of this inhibition, we ectopically expressed Goosecoid in erythroleukemia cells. While erythroid differentiation of these cells can be induced by activin, Goosecoid expressing cells were unresponsive to activin. We demonstrate an in vitro interaction between the oncogene PU.1, an ets family transcription factor thought to play a role in erythropoiesis, and the Goosecoid Protein (GSC). Interaction with PU.1 was specific as GSC did not bind to the ets family members, Fli-1 or Ets-2. The ability of Goosecoid expressing erythroleukemia cells to differentiate in response to activin was rescued by coexpression of the GSC-binding N-terminal portion of PU.1. The N-terminal portion of PU.1 was co-immunoprecipitated with anti-GSC antibodies as well. The N-terminal domain of PU.1 is the region recognized by the retinoblastoma Protein (Rb), a tumor suppressor gene presumably involved in erythroid differentiation. We show that GSC competitively inhibits binding of Rb to PU.1. Our data suggest that the suppression of blood formation by GSC could, at least in part, be mediated by binding to PU.1.
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Negative Autoregulation of the Organizer-specific Homeobox Gene Goosecoid
Journal of Biological Chemistry, 1998Co-Authors: Vlatko Danilov, Martin Blum, Axel Schweickert, Marina Campione, Herbert SteinbeisserAbstract:The homeobox gene Goosecoid has been implicated to play a central role in the Spemann organizer tissue of the vertebrate embryo. Misexpression of Goosecoid on the ventral side of a Xenopus laevis gastrula embryo was shown to result in a partial duplication of the primary body axis, reminiscent of the Spemann organizer graft. Normal embryonic development thus requires tight temporal and spatial control of genes instrumental for organizer function. In the present study we investigated the transcriptional control of Goosecoid gene expression. Sequence analysis of the mouse and human promoter region revealed the presence of two palindromic binding elements for homeobox genes of the prd type to which Goosecoid belongs. We show that Goosecoid Protein can bind to these sites in vitro. By using reporter gene constructs of the human and mouse promoter, we demonstrate that Goosecoid can act as a repressor of its own promoter activity in transient co-transfection experiments in mouse P19 cells and in Xenopus embryos. Autorepression depends on the presence of the homeodomain and is mediated through the prd element more proximal to the transcriptional start site. Our results suggest a role for Goosecoid in restricting organizer activity in the vertebrate gastrula embryo.
Axel Schweickert - One of the best experts on this subject based on the ideXlab platform.
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Negative Autoregulation of the Organizer-specific Homeobox Gene Goosecoid
Journal of Biological Chemistry, 1998Co-Authors: Vlatko Danilov, Martin Blum, Axel Schweickert, Marina Campione, Herbert SteinbeisserAbstract:The homeobox gene Goosecoid has been implicated to play a central role in the Spemann organizer tissue of the vertebrate embryo. Misexpression of Goosecoid on the ventral side of a Xenopus laevis gastrula embryo was shown to result in a partial duplication of the primary body axis, reminiscent of the Spemann organizer graft. Normal embryonic development thus requires tight temporal and spatial control of genes instrumental for organizer function. In the present study we investigated the transcriptional control of Goosecoid gene expression. Sequence analysis of the mouse and human promoter region revealed the presence of two palindromic binding elements for homeobox genes of the prd type to which Goosecoid belongs. We show that Goosecoid Protein can bind to these sites in vitro. By using reporter gene constructs of the human and mouse promoter, we demonstrate that Goosecoid can act as a repressor of its own promoter activity in transient co-transfection experiments in mouse P19 cells and in Xenopus embryos. Autorepression depends on the presence of the homeodomain and is mediated through the prd element more proximal to the transcriptional start site. Our results suggest a role for Goosecoid in restricting organizer activity in the vertebrate gastrula embryo.
Marina Campione - One of the best experts on this subject based on the ideXlab platform.
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Negative Autoregulation of the Organizer-specific Homeobox Gene Goosecoid
Journal of Biological Chemistry, 1998Co-Authors: Vlatko Danilov, Martin Blum, Axel Schweickert, Marina Campione, Herbert SteinbeisserAbstract:The homeobox gene Goosecoid has been implicated to play a central role in the Spemann organizer tissue of the vertebrate embryo. Misexpression of Goosecoid on the ventral side of a Xenopus laevis gastrula embryo was shown to result in a partial duplication of the primary body axis, reminiscent of the Spemann organizer graft. Normal embryonic development thus requires tight temporal and spatial control of genes instrumental for organizer function. In the present study we investigated the transcriptional control of Goosecoid gene expression. Sequence analysis of the mouse and human promoter region revealed the presence of two palindromic binding elements for homeobox genes of the prd type to which Goosecoid belongs. We show that Goosecoid Protein can bind to these sites in vitro. By using reporter gene constructs of the human and mouse promoter, we demonstrate that Goosecoid can act as a repressor of its own promoter activity in transient co-transfection experiments in mouse P19 cells and in Xenopus embryos. Autorepression depends on the presence of the homeodomain and is mediated through the prd element more proximal to the transcriptional start site. Our results suggest a role for Goosecoid in restricting organizer activity in the vertebrate gastrula embryo.
