The Experts below are selected from a list of 363 Experts worldwide ranked by ideXlab platform
Sueg-geun Lee - One of the best experts on this subject based on the ideXlab platform.
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Communications Pulsed Field Gradient Long-range COSY Experiment: Combined Use of Gradient and Fixed Delay for the Detection of Long-range Couplings
2001Co-Authors: Sueg-geun LeeAbstract:Since the Gradients probe-heads have been commercially available in 1991, many Gradient-based experiments are spanning a range of applications which are useful in resolving many chemical problems.1-3 Among the many advantages of using Gradient in NMR experiments, the most significant aspect is the coherence pathway selection using Gradient pulses instead of phase cycling.4 Therefore, the data acquisition time can be significantly decreased for the properly concen-trated samples. The Gradient experiments are well establish-ed for the normal vicinal coupling constants.5-6 However, the long-range coupling experiments (> 3J) are rarely applied in the field of Gradient-based experiments. The earlier experi-ment of the weaker long-range 4J and 5J proton connec-tivities7 has only been observed in Gradient COSY experi-ment by the changes of Gradient strength and Gradient time.
Marky Luis - One of the best experts on this subject based on the ideXlab platform.
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Binding of ethidium to DNA measured using a 2D diffusion-modulated Gradient COSY NMR experiment
Federation of European Biochemical Societies. Published by Elsevier B.V., 2000Co-Authors: Gmeiner, William H., Hudalla, Christopher J., Soto, Ana Maria, Marky LuisAbstract:AbstractThe binding of ethidium bromide to a DNA hairpin (dU5-hairpin) was investigated using a novel 2D diffusion-modulated Gradient correlation spectroscopy (DMG-COSY) experiment to evaluate the applicability of this technique for studying the binding of drugs to DNA. The DMG-COSY experiment includes a preparation period during which coherent magnetization is attenuated due to molecular self-diffusion. Magnetization then evolves due to scalar coupling during an evolution delay, and is detected using Gradient pulses for coherence selection. The time-domain data are processed in an analogous manner as for Gradient-selected COSY experiments. The diffusion coefficient for uridine in DMSO solution was determined from the H5–H6 crosspeak intensities for a series of 2D DMG-COSY experiments that differed in the magnitude of the Gradient pulses applied during the preparation period of the DMG-COSY experiment. The diffusion coefficient for uridine calculated from the DMG-COSY experiments was identical (within experimental error) to that determined from 1D diffusion experiments (5.24×10−6 cm2/s at 26°C). The diffusion coefficients for ethidium bromide and for the dU5-hairpin were first measured separately using the DMG-COSY experiment, and then measured in the putative complex. The diffusion coefficient for free ethidium bromide (4.15×10−6 cm2/s at 26°C) was considerably larger than for the dU5-hairpin (1.60×10−6 cm2/s at 26°C), as expected for the smaller molecule. The diffusion coefficient for ethidium was markedly decreased upon addition of the dU5-hairpin, consistent with complex formation (1.22×10−6 cm2/s at 26°C). Complex formation of 1:1 stoichiometry between ethidium and the stem of the dU5-hairpin was verified independently by fluorescence spectroscopy. These results demonstrate the utility of the DMG-COSY experiment for investigating the binding of drugs to DNA in aqueous solution
Gmeiner, William H. - One of the best experts on this subject based on the ideXlab platform.
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Binding of ethidium to DNA measured using a 2D diffusion-modulated Gradient COSY NMR experiment
Federation of European Biochemical Societies. Published by Elsevier B.V., 2000Co-Authors: Gmeiner, William H., Hudalla, Christopher J., Soto, Ana Maria, Marky LuisAbstract:AbstractThe binding of ethidium bromide to a DNA hairpin (dU5-hairpin) was investigated using a novel 2D diffusion-modulated Gradient correlation spectroscopy (DMG-COSY) experiment to evaluate the applicability of this technique for studying the binding of drugs to DNA. The DMG-COSY experiment includes a preparation period during which coherent magnetization is attenuated due to molecular self-diffusion. Magnetization then evolves due to scalar coupling during an evolution delay, and is detected using Gradient pulses for coherence selection. The time-domain data are processed in an analogous manner as for Gradient-selected COSY experiments. The diffusion coefficient for uridine in DMSO solution was determined from the H5–H6 crosspeak intensities for a series of 2D DMG-COSY experiments that differed in the magnitude of the Gradient pulses applied during the preparation period of the DMG-COSY experiment. The diffusion coefficient for uridine calculated from the DMG-COSY experiments was identical (within experimental error) to that determined from 1D diffusion experiments (5.24×10−6 cm2/s at 26°C). The diffusion coefficients for ethidium bromide and for the dU5-hairpin were first measured separately using the DMG-COSY experiment, and then measured in the putative complex. The diffusion coefficient for free ethidium bromide (4.15×10−6 cm2/s at 26°C) was considerably larger than for the dU5-hairpin (1.60×10−6 cm2/s at 26°C), as expected for the smaller molecule. The diffusion coefficient for ethidium was markedly decreased upon addition of the dU5-hairpin, consistent with complex formation (1.22×10−6 cm2/s at 26°C). Complex formation of 1:1 stoichiometry between ethidium and the stem of the dU5-hairpin was verified independently by fluorescence spectroscopy. These results demonstrate the utility of the DMG-COSY experiment for investigating the binding of drugs to DNA in aqueous solution
Hudalla, Christopher J. - One of the best experts on this subject based on the ideXlab platform.
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Binding of ethidium to DNA measured using a 2D diffusion-modulated Gradient COSY NMR experiment
Federation of European Biochemical Societies. Published by Elsevier B.V., 2000Co-Authors: Gmeiner, William H., Hudalla, Christopher J., Soto, Ana Maria, Marky LuisAbstract:AbstractThe binding of ethidium bromide to a DNA hairpin (dU5-hairpin) was investigated using a novel 2D diffusion-modulated Gradient correlation spectroscopy (DMG-COSY) experiment to evaluate the applicability of this technique for studying the binding of drugs to DNA. The DMG-COSY experiment includes a preparation period during which coherent magnetization is attenuated due to molecular self-diffusion. Magnetization then evolves due to scalar coupling during an evolution delay, and is detected using Gradient pulses for coherence selection. The time-domain data are processed in an analogous manner as for Gradient-selected COSY experiments. The diffusion coefficient for uridine in DMSO solution was determined from the H5–H6 crosspeak intensities for a series of 2D DMG-COSY experiments that differed in the magnitude of the Gradient pulses applied during the preparation period of the DMG-COSY experiment. The diffusion coefficient for uridine calculated from the DMG-COSY experiments was identical (within experimental error) to that determined from 1D diffusion experiments (5.24×10−6 cm2/s at 26°C). The diffusion coefficients for ethidium bromide and for the dU5-hairpin were first measured separately using the DMG-COSY experiment, and then measured in the putative complex. The diffusion coefficient for free ethidium bromide (4.15×10−6 cm2/s at 26°C) was considerably larger than for the dU5-hairpin (1.60×10−6 cm2/s at 26°C), as expected for the smaller molecule. The diffusion coefficient for ethidium was markedly decreased upon addition of the dU5-hairpin, consistent with complex formation (1.22×10−6 cm2/s at 26°C). Complex formation of 1:1 stoichiometry between ethidium and the stem of the dU5-hairpin was verified independently by fluorescence spectroscopy. These results demonstrate the utility of the DMG-COSY experiment for investigating the binding of drugs to DNA in aqueous solution
Soto, Ana Maria - One of the best experts on this subject based on the ideXlab platform.
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Binding of ethidium to DNA measured using a 2D diffusion-modulated Gradient COSY NMR experiment
Federation of European Biochemical Societies. Published by Elsevier B.V., 2000Co-Authors: Gmeiner, William H., Hudalla, Christopher J., Soto, Ana Maria, Marky LuisAbstract:AbstractThe binding of ethidium bromide to a DNA hairpin (dU5-hairpin) was investigated using a novel 2D diffusion-modulated Gradient correlation spectroscopy (DMG-COSY) experiment to evaluate the applicability of this technique for studying the binding of drugs to DNA. The DMG-COSY experiment includes a preparation period during which coherent magnetization is attenuated due to molecular self-diffusion. Magnetization then evolves due to scalar coupling during an evolution delay, and is detected using Gradient pulses for coherence selection. The time-domain data are processed in an analogous manner as for Gradient-selected COSY experiments. The diffusion coefficient for uridine in DMSO solution was determined from the H5–H6 crosspeak intensities for a series of 2D DMG-COSY experiments that differed in the magnitude of the Gradient pulses applied during the preparation period of the DMG-COSY experiment. The diffusion coefficient for uridine calculated from the DMG-COSY experiments was identical (within experimental error) to that determined from 1D diffusion experiments (5.24×10−6 cm2/s at 26°C). The diffusion coefficients for ethidium bromide and for the dU5-hairpin were first measured separately using the DMG-COSY experiment, and then measured in the putative complex. The diffusion coefficient for free ethidium bromide (4.15×10−6 cm2/s at 26°C) was considerably larger than for the dU5-hairpin (1.60×10−6 cm2/s at 26°C), as expected for the smaller molecule. The diffusion coefficient for ethidium was markedly decreased upon addition of the dU5-hairpin, consistent with complex formation (1.22×10−6 cm2/s at 26°C). Complex formation of 1:1 stoichiometry between ethidium and the stem of the dU5-hairpin was verified independently by fluorescence spectroscopy. These results demonstrate the utility of the DMG-COSY experiment for investigating the binding of drugs to DNA in aqueous solution
