The Experts below are selected from a list of 90 Experts worldwide ranked by ideXlab platform
Jan Schmidt - One of the best experts on this subject based on the ideXlab platform.
-
reduction of ischemia reperfusion injury by antithrombin iii after experimental pancreas transplantation
American Journal of Surgery, 2005Co-Authors: Thilo Hackert, Jens Werner, Marthamaria Gebhard, Markus W Buchler, Jan SchmidtAbstract:Abstract Background Graft Pancreatitis is a major complication after pancreas transplantation. Antithrombin III (AT III) is an anticoagulatory and anti-inflammatory substance. The aim of our study was to evaluate a prophylactic application of AT III in experimental pancreas transplantation. Methods Pancreas transplantation was performed in rats. Cold ischemia time (University of Wisconsin solution at 4°C) was 12 hours. After 4 hours of reperfusion, pancreatic enzymes were assessed and the pancreas was evaluated by intravital microscopy and histologic and immunohistochemical examination. Recipients were allocated randomly to 2 groups: 1 control group (n = 6) and 1 group in which recipients received 125 IU AT III/kg 30 minutes before reperfusion (n = 6). Six animals that did not undergo transplantation served as healthy controls. Results Enzyme levels showed no differences between the 2 transplantation groups but were significantly ( P Conclusions AT III pretreatment decreases tissue damage by attenuating microcirculatory disturbances and leukocyte adherence in experimental Graft Pancreatitis by its anti-inflammatory and anticoagulatory properties.
-
characterization and reduction of ischemia reperfusion injury after experimental pancreas transplantation
Journal of Gastrointestinal Surgery, 1999Co-Authors: H Mayer, Marthamaria Gebhard, Jan Schmidt, J C Thies, Eduard Ryschich, Ch Herfarth, E KlarAbstract:Reperfusion injury after pancreas transplantation is a cause of early Graft Pancreatitis. The aim of this study was to quantify pancreatic microcirculation after pancreas transplantation in correlation with cold ischemia time. In a second step the effect of N-acetylcysteine on reperfusion damage was tested. Pancreas transplantation was performed in three different groups of male Lewis rats. Groups 1 and 2 received no special treatment. Cold ischemia time was 1.5 hours in group 1 and 16 hours in groups 2 and 3. In group 3 donor and recipient were both treated with N-acetylcysteine (300 mg/kg) 1.5 hours after reperfusion Graft microcirculation was quantified by means of intravital microscopy. Rhodamine-labeled leukocytes, fluoroscein isothiocyanate-labeled erythrocytes, and fluoroscein isothiocyanate-albumin were used as fluorochromes. After a cold ischemia time of 16 hours, functional capillary density, erythrocyte velocity, and leukocyte-endothelium interaction were reduced significantly compared to a cold ischemia time of 1.5 hours (P <0.05). After 16 hours of cold ischemia, treatment with N-acetylcysteine improved all of these parameters (P ≤ 0.05). Ischemia/reperfusion injury after experimental pancreas transplantation is characterized by a disturbance of the pancreatic microcirculation exhibiting a correlation with the duration of cold ischemia. Treatment of donor and recipient with N-acetylcysteine resulted in prevention of cold ischemia-induced microcirculatory disturbance.
Yoshikazu Kuroda - One of the best experts on this subject based on the ideXlab platform.
-
possible role of heat shock protein 60 in reducing ischemic reperfusion injury in canine pancreas Grafts after preservation by the two layer method
Pancreas, 2001Co-Authors: Yasuhiro Fujino, Yasuyuki Suzuki, Yasuki Tanioka, Toshiaki Tsujimura, Tsuyoshi Takahashi, Masahiro Tominaga, Yoshikazu KurodaAbstract:Introduction: Recently, results of the clinical application of the two-layer method have shown the morphologic quality of the human pancreas Grafts after reperfusion to be excellent, although ischemia-reperfusion injury is related to early Graft loss in pancreas transplantation. However, some reports have indicated that heat shock proteins (HSPs) have important functions in response to the stress-related events. Aim: To examine whether the two-layer method reduced ischemia-reperfusion injury in a canine pancreas autotransplantation model by investigating the expression of HSPs. Methodology: There were three experimental groups in which dogs received segmental autoGrafts after preservation by the two-layer method using University of Wisconsin solution (UW) (group 1), simple storage in UW (group 2) for 24 hours, or no preservation (group 3). Results: In group 1, pancreatic tissue perfusions were high, and pancreatic exocrine functions were well preserved after 1, 2, and 4 hours of reperfusion with low incidence of Graft Pancreatitis or vessel thrombosis compared with that in group 2. Moreover, ATP rapidly recovered, and HSP 60 was strongly enhanced after reperfusion in group 1. On the other hand, ATP recovery and the enhancement of HSP 60 were weak after reperfusion in group 2. Conclusion: The two-layer method reduced ischemia-reperfusion injury compared with UW simple storage in canine pancreas autotransplantation with a strong expression of HSP 60.
-
peripancreatic fluid cytology detection of early rejection versus Graft Pancreatitis after canine pancreatic transplantation
World Journal of Surgery, 1997Co-Authors: Yasuyuki Suzuki, Yoshikazu Kuroda, Yasuki Tanioka, S Matsumoto, Yongsik Kim, Ryuko Tsukamoto, Yoichi SaitohAbstract:n= 25) or autoGraft (n= 37) heterotopically in the neck. This study included five groups: alloGrafts without immunosuppression (group A,n= 12), alloGrafts with immunosuppression (group B,n= 13), autoGrafts without immunosuppression (group C, n= 11), autoGrafts with immunosuppression (group D, n= 12), and autoGrafts treated by 45 minutes of pretransplant warm ischemia to induce acute Graft Pancreatitis (group E, n= 14). A closed suction drainage catheter was placed next to the Graft to collect peripancreatic fluid daily after the transplant. PFC was performed using May-Gruenwald-Giemsa double-staining technique and compared to the corresponding histology through the observation period. In analyses of 50 functioning Grafts, PFC performed on day 1 showed similar neutrophil accumulations in all groups. In sharp contrast, on days 3 and 6, group A had dramatically increased mononuclear cell concentrations in PFC, whereas groups B, C, and D showed significantly lower concentrations, the percent of mononuclear cells among total leukocytes being 47.3 ± 23.4%, 11.8 ± 4.9%, 4.3 ± 1.8%, and 6.4 ± 2.4% (day 3); and 32.7 ± 9.8%, 10.5 ± 4.8%, 7.2 ± 4.2%, and 8.6 ± 6.4% (day 6) in groups A, B, C, and D, respectively. On the other hand, in group E numerous degenerating neutrophils with a marked to moderate increase in necrotic tissue fragments were observed by PFC on days 3 and 6. In terms of Graft histology on days 3 and 6, group A showed interstitial mononuclear cell infiltration indicating an acute rejection process, whereas groups B, C, and D had minimal inflammatory cell infiltration. In group E Graft Pancreatitis was histologically confirmed on days 3 and 6. These results suggest that PFC after pancreas transplantation could be a safe, simple, useful diagnostic tool for discriminating early Graft rejection from Graft Pancreatitis.
E Klar - One of the best experts on this subject based on the ideXlab platform.
-
characterization and reduction of ischemia reperfusion injury after experimental pancreas transplantation
Journal of Gastrointestinal Surgery, 1999Co-Authors: H Mayer, Marthamaria Gebhard, Jan Schmidt, J C Thies, Eduard Ryschich, Ch Herfarth, E KlarAbstract:Reperfusion injury after pancreas transplantation is a cause of early Graft Pancreatitis. The aim of this study was to quantify pancreatic microcirculation after pancreas transplantation in correlation with cold ischemia time. In a second step the effect of N-acetylcysteine on reperfusion damage was tested. Pancreas transplantation was performed in three different groups of male Lewis rats. Groups 1 and 2 received no special treatment. Cold ischemia time was 1.5 hours in group 1 and 16 hours in groups 2 and 3. In group 3 donor and recipient were both treated with N-acetylcysteine (300 mg/kg) 1.5 hours after reperfusion Graft microcirculation was quantified by means of intravital microscopy. Rhodamine-labeled leukocytes, fluoroscein isothiocyanate-labeled erythrocytes, and fluoroscein isothiocyanate-albumin were used as fluorochromes. After a cold ischemia time of 16 hours, functional capillary density, erythrocyte velocity, and leukocyte-endothelium interaction were reduced significantly compared to a cold ischemia time of 1.5 hours (P <0.05). After 16 hours of cold ischemia, treatment with N-acetylcysteine improved all of these parameters (P ≤ 0.05). Ischemia/reperfusion injury after experimental pancreas transplantation is characterized by a disturbance of the pancreatic microcirculation exhibiting a correlation with the duration of cold ischemia. Treatment of donor and recipient with N-acetylcysteine resulted in prevention of cold ischemia-induced microcirculatory disturbance.
Marthamaria Gebhard - One of the best experts on this subject based on the ideXlab platform.
-
reduction of ischemia reperfusion injury by antithrombin iii after experimental pancreas transplantation
American Journal of Surgery, 2005Co-Authors: Thilo Hackert, Jens Werner, Marthamaria Gebhard, Markus W Buchler, Jan SchmidtAbstract:Abstract Background Graft Pancreatitis is a major complication after pancreas transplantation. Antithrombin III (AT III) is an anticoagulatory and anti-inflammatory substance. The aim of our study was to evaluate a prophylactic application of AT III in experimental pancreas transplantation. Methods Pancreas transplantation was performed in rats. Cold ischemia time (University of Wisconsin solution at 4°C) was 12 hours. After 4 hours of reperfusion, pancreatic enzymes were assessed and the pancreas was evaluated by intravital microscopy and histologic and immunohistochemical examination. Recipients were allocated randomly to 2 groups: 1 control group (n = 6) and 1 group in which recipients received 125 IU AT III/kg 30 minutes before reperfusion (n = 6). Six animals that did not undergo transplantation served as healthy controls. Results Enzyme levels showed no differences between the 2 transplantation groups but were significantly ( P Conclusions AT III pretreatment decreases tissue damage by attenuating microcirculatory disturbances and leukocyte adherence in experimental Graft Pancreatitis by its anti-inflammatory and anticoagulatory properties.
-
characterization and reduction of ischemia reperfusion injury after experimental pancreas transplantation
Journal of Gastrointestinal Surgery, 1999Co-Authors: H Mayer, Marthamaria Gebhard, Jan Schmidt, J C Thies, Eduard Ryschich, Ch Herfarth, E KlarAbstract:Reperfusion injury after pancreas transplantation is a cause of early Graft Pancreatitis. The aim of this study was to quantify pancreatic microcirculation after pancreas transplantation in correlation with cold ischemia time. In a second step the effect of N-acetylcysteine on reperfusion damage was tested. Pancreas transplantation was performed in three different groups of male Lewis rats. Groups 1 and 2 received no special treatment. Cold ischemia time was 1.5 hours in group 1 and 16 hours in groups 2 and 3. In group 3 donor and recipient were both treated with N-acetylcysteine (300 mg/kg) 1.5 hours after reperfusion Graft microcirculation was quantified by means of intravital microscopy. Rhodamine-labeled leukocytes, fluoroscein isothiocyanate-labeled erythrocytes, and fluoroscein isothiocyanate-albumin were used as fluorochromes. After a cold ischemia time of 16 hours, functional capillary density, erythrocyte velocity, and leukocyte-endothelium interaction were reduced significantly compared to a cold ischemia time of 1.5 hours (P <0.05). After 16 hours of cold ischemia, treatment with N-acetylcysteine improved all of these parameters (P ≤ 0.05). Ischemia/reperfusion injury after experimental pancreas transplantation is characterized by a disturbance of the pancreatic microcirculation exhibiting a correlation with the duration of cold ischemia. Treatment of donor and recipient with N-acetylcysteine resulted in prevention of cold ischemia-induced microcirculatory disturbance.
J Hauss - One of the best experts on this subject based on the ideXlab platform.
-
endothelina receptor blockade reduces ischemia reperfusion injury in pig pancreas transplantation
Annals of Surgery, 2003Co-Authors: Helmut Witzigmann, Stefan Ludwig, Barbara Armann, Gabor Gabel, Daniel Teupser, Jurgen Kratzsch, Uta Carolin Pietsch, Andrea Tannapfel, F Geissler, J HaussAbstract:Objective: The effect of prophylactic administration of a selective endothelinA receptor antagonist (ETA-RA) on ischemia/reperfusion injury in an experimental model of Graft Pancreatitis after pancreas transplantation was evaluated.
