The Experts below are selected from a list of 174 Experts worldwide ranked by ideXlab platform
Yvon Berland - One of the best experts on this subject based on the ideXlab platform.
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Effect of uremia and hemodialysis on soluble L-selectin and leukocyte surface CD11b and L-selectin
American Journal of Kidney Diseases, 1998Co-Authors: Philippe Brunet, Françoise Dignat-george, José Sampol, Yvon BerlandAbstract:Abstract Leukocyte Mac-1 (CD11b/CD18) and L-selectin (CD62L) are implicated in leukocyte adhesion to endothelial cells. In this study, L-selectin and CD11b expression on leukocytes and soluble L-selectin (sL-selectin) serum levels were investigated in 17 nondialyzed patients with chronic renal failure (CRF), in 28 chronic hemodialysis patients before hemodialysis (basal state), and in 32 healthy subjects. These parameters were also monitored during hemodialysis with cuprophane and cellulose diacetate membranes in a crossover study in five patients. Granulocytes from CRF patients displayed lower expression of L-selectin and higher expression of CD11b than Granulocytes from healthy subjects. On the other hand, baseline expression of L-selectin and CD11b on leukocytes from hemodialysis patients did not differ from that of healthy subjects. In CRF and hemodialysis patients, sL-selectin levels were significantly lower than in healthy subjects. During hemodialysis, cuprophane membrane induced an upregulation of Granulocyte CD11b, a decrease in Granulocyte L-selectin, and an increase in sL-selectin serum levels. Conversely, cellulose diacetate caused only a transient increase in Granulocyte CD11b and did not modify Granulocyte L-selectin and sL-selectin serum levels. High CD11b and low L-selectin expression on Granulocytes in CRF patients suggests an activation state, which was not found in hemodialysis patients at the basal state. The lack of activation in hemodialysis patients could reflect the elimination of a uremic toxin by dialysis or a loss of Granulocyte responsiveness because of the repetitive stimulation by hemodialysis treatment. The low serum levels of sL-selectin in CRF and hemodialysis patients also suggest Granulocyte dysfunction. (Am J Kidney Dis 1998 Jan;31(1):67-73)
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Effect of uremia and hemodialysis on soluble L-selectin and leukocyte surface CD11b and L-selectin
American Journal of Kidney Diseases, 1998Co-Authors: Laetitia Dou, Philippe Brunet, Françoise Dignat-george, José Sampol, Yvon BerlandAbstract:Leukocyte Mac-1 (CD11b/CD18) and L-selectin (CD62L) are implicated in leukocyte adhesion to endothelial cells. In this study, L-selectin and CD11b expression on leukocytes and soluble L-selectin (sL-selectin) serum levels were investigated in 17 nondialyzed patients with chronic renal failure (CRF), in 28 chronic hemodialysis patients before hemodialysis (basal state), and in 32 healthy subjects. These parameters were also monitored during hemodialysis with cuprophane and cellulose diacetate membranes in a crossover study in five patients. Granulocytes from CRF patients displayed lower expression of L-selectin and higher expression of CD11b than Granulocytes from healthy subjects. On the other hand, baseline expression of L-selectin and CD11b on leukocytes from hemodialysis patients did not differ from that of healthy subjects. In CRF and hemodialysis patients, sL-selectin levels were significantly lower than in healthy subjects. During hemodialysis, cuprophane membrane induced an upregulation of Granulocyte CD11b, a decrease in Granulocyte L-selectin, and an increase in sL-selectin serum levels. Conversely, cellulose diacetate caused only a transient increase in Granulocyte CD11b and did not modify Granulocyte L-selectin and sL-selectin serum levels. High CD11b and low L-selectin expression on Granulocytes in CRF patients suggests an activation state, which was not found in hemodialysis patients at the basal state. The lack of activation in hemodialysis patients could reflect the elimination of a uremic toxin by dialysis or a loss of Granulocyte responsiveness because of the repetitive stimulation by hemodialysis treatment. The low serum levels of sL-selectin in CRF and hemodialysis patients also suggest Granulocyte dysfunction. (C) 1998 by the National Kidney Foundation, Inc.
Thomas H. Price - One of the best experts on this subject based on the ideXlab platform.
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Granulocyte transfusion in the G-CSF era.
International Journal of Hematology, 2002Co-Authors: Thomas H. PriceAbstract:Granulocyte transfusions have been used since the 1960s with varying degrees of clinical success in the treatment of infection in patients with neutropenia or inherited Granulocyte disorders. A number of studies have indicated that efficacy may well be associated with the dose of Granulocytes delivered. Collection of Granulocytes using modern apheresis machines and corticosteroid administration yields approximately 20∼30×109 neutrophils, unlikely to be adequate for treating an established infection. The administration of G-CSF to healthy donors has resulted in average Granulocyte yields up to 8×1010 cells. Normal or near normal blood neutrophil counts are often attained when these concentrates are transfused to neutropenic recipients, and these levels are sustained for up to 24 h. G-CSF-primed Granulocytes appear to be functionally normal by both in vitro and in vivo measurements. Adverse effects experienced by recipients are similar to those seen with traditional doses of Granulocytes. G-CSF administration to donors is well tolerated. Controlled clinical trials are needed to determine the therapeutic efficacy of G-CSF-primed Granulocyte transfusions.
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Granulocyte transfusion in the G-CSF era.
International journal of hematology, 2002Co-Authors: Thomas H. PriceAbstract:Granulocyte transfusions have been used since the 1960s with varying degrees of clinical success in the treatment of infection in patients with neutropenia or inherited Granulocyte disorders. A number of studies have indicated that efficacy may well be associated with the dose of Granulocytes delivered. Collection of Granulocytes using modern apheresis machines and corticosteroid administration yields approximately 20 to approximately 30 x 10(9) neutrophils, unlikely to be adequate for treating an established infection. The administration of G-CSF to healthy donors has resulted in average Granulocyte yields up to 8 x 10(10) cells. Normal or near normal blood neutrophil counts are often attained when these concentrates are transfused to neutropenic recipients, and these levels are sustained for up to 24 h. G-CSF-primed Granulocytes appear to be functionally normal by both in vitro and in vivo measurements. Adverse effects experienced by recipients are similar to those seen with traditional doses of Granulocytes. G-CSF administration to donors is well tolerated. Controlled clinical trials are needed to determine the therapeutic efficacy of G-CSF-primed Granulocyte transfusions.
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Granulocyte colony-stimulating factor-mobilized Granulocyte concentrate transfusions
Current opinion in hematology, 1998Co-Authors: Thomas H. PriceAbstract:Granulocyte transfusion therapy has been used infrequently in the last 10 to 15 years, in large part because its efficacy in the treatment of infected neutropenic patients has not been impressive. This perceived lack of efficacy has been attributed primarily to the fact that the dose of Granulocytes obtainable with standard leukapheresis techniques has been inadequate. With the availability of recombinant Granulocyte colony-stimulating factor (G-CSF) to stimulate neutrophilia in normal donors and increase the number of Granulocytes that can be collected, there is now renewed interest in this form of transfusion therapy. Recent studies have shown that stimulation with G-CSF, with or without corticosteroids, is well tolerated by normal donors and that Granulocyte yields are increased three- to four-fold. Blood neutrophil counts in patients receiving these large cell doses rise substantially, often to normal or near normal levels, and commonly remain elevated for 24 hours or more. In vitro and in vivo measurements have shown that the functional capabilities of Granulocytes collected from G-CSF stimulated donors appear to be normal. Although early reports have been encouraging, the clinical efficacy of this new level of Granulocyte transfusion therapy has been yet to be determined.
Richard H. Gomer - One of the best experts on this subject based on the ideXlab platform.
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Serum amyloid P inhibits Granulocyte adhesion.
Fibrogenesis & Tissue Repair, 2013Co-Authors: Anu S. Maharjan, David Roife, Derrick Brazill, Richard H. GomerAbstract:Background The extravasation of Granulocytes (such as neutrophils) at a site of inflammation is a key aspect of the innate immune system. Signals from the site of inflammation upregulate Granulocyte adhesion to the endothelium to initiate extravasation, and also enhance Granulocyte adhesion to extracellular matrix proteins to facilitate Granulocyte movement through the inflamed tissue. During the resolution of inflammation, other signals inhibit Granulocyte adhesion to slow and ultimately stop Granulocyte influx into the tissue. In a variety of inflammatory diseases such as acute respiratory distress syndrome, an excess infiltration of Granulocytes into a tissue causes undesired collateral damage, and being able to reduce Granulocyte adhesion and influx could reduce this damage.
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Serum amyloid P inhibits Granulocyte adhesion.
Fibrogenesis & tissue repair, 2013Co-Authors: Anu S. Maharjan, David Roife, Derrick Brazill, Richard H. GomerAbstract:The extravasation of Granulocytes (such as neutrophils) at a site of inflammation is a key aspect of the innate immune system. Signals from the site of inflammation upregulate Granulocyte adhesion to the endothelium to initiate extravasation, and also enhance Granulocyte adhesion to extracellular matrix proteins to facilitate Granulocyte movement through the inflamed tissue. During the resolution of inflammation, other signals inhibit Granulocyte adhesion to slow and ultimately stop Granulocyte influx into the tissue. In a variety of inflammatory diseases such as acute respiratory distress syndrome, an excess infiltration of Granulocytes into a tissue causes undesired collateral damage, and being able to reduce Granulocyte adhesion and influx could reduce this damage. We found that serum amyloid P (SAP), a constitutive protein component of the blood, inhibits Granulocyte spreading and Granulocyte adhesion to extracellular matrix components. This indicates that in addition to Granulocyte adhesion inhibitors that are secreted during the resolution of inflammation, a Granulocyte adhesion inhibitor is present at all times in the blood. Although SAP affects adhesion, it does not affect the Granulocyte adhesion molecules CD11b, CD62L, CD18, or CD44. SAP also has no effect on the production of hydrogen peroxide by resting or stimulated Granulocytes, or N-formyl-methionine-leucine-phenylalanine (fMLP)-induced Granulocyte migration. In mice treated with intratracheal bleomycin to induce Granulocyte accumulation in the lungs, SAP injections reduced the number of Granulocytes in the lungs. We found that SAP, a constitutive component of blood, is a Granulocyte adhesion inhibitor. We hypothesize that SAP allows Granulocytes to sense whether they are in the blood or in a tissue.
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Serum amyloid P inhibits Granulocyte adhesion
Fibrogenesis & Tissue Repair, 2013Co-Authors: Anu S. Maharjan, David Roife, Derrick Brazill, Richard H. GomerAbstract:Background The extravasation of Granulocytes (such as neutrophils) at a site of inflammation is a key aspect of the innate immune system. Signals from the site of inflammation upregulate Granulocyte adhesion to the endothelium to initiate extravasation, and also enhance Granulocyte adhesion to extracellular matrix proteins to facilitate Granulocyte movement through the inflamed tissue. During the resolution of inflammation, other signals inhibit Granulocyte adhesion to slow and ultimately stop Granulocyte influx into the tissue. In a variety of inflammatory diseases such as acute respiratory distress syndrome, an excess infiltration of Granulocytes into a tissue causes undesired collateral damage, and being able to reduce Granulocyte adhesion and influx could reduce this damage. Results We found that serum amyloid P (SAP), a constitutive protein component of the blood, inhibits Granulocyte spreading and Granulocyte adhesion to extracellular matrix components. This indicates that in addition to Granulocyte adhesion inhibitors that are secreted during the resolution of inflammation, a Granulocyte adhesion inhibitor is present at all times in the blood. Although SAP affects adhesion, it does not affect the Granulocyte adhesion molecules CD11b, CD62L, CD18, or CD44. SAP also has no effect on the production of hydrogen peroxide by resting or stimulated Granulocytes, or N -formyl-methionine-leucine-phenylalanine (fMLP)-induced Granulocyte migration. In mice treated with intratracheal bleomycin to induce Granulocyte accumulation in the lungs, SAP injections reduced the number of Granulocytes in the lungs. Conclusions We found that SAP, a constitutive component of blood, is a Granulocyte adhesion inhibitor. We hypothesize that SAP allows Granulocytes to sense whether they are in the blood or in a tissue.
Philippe Brunet - One of the best experts on this subject based on the ideXlab platform.
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Effect of uremia and hemodialysis on soluble L-selectin and leukocyte surface CD11b and L-selectin
American Journal of Kidney Diseases, 1998Co-Authors: Philippe Brunet, Françoise Dignat-george, José Sampol, Yvon BerlandAbstract:Abstract Leukocyte Mac-1 (CD11b/CD18) and L-selectin (CD62L) are implicated in leukocyte adhesion to endothelial cells. In this study, L-selectin and CD11b expression on leukocytes and soluble L-selectin (sL-selectin) serum levels were investigated in 17 nondialyzed patients with chronic renal failure (CRF), in 28 chronic hemodialysis patients before hemodialysis (basal state), and in 32 healthy subjects. These parameters were also monitored during hemodialysis with cuprophane and cellulose diacetate membranes in a crossover study in five patients. Granulocytes from CRF patients displayed lower expression of L-selectin and higher expression of CD11b than Granulocytes from healthy subjects. On the other hand, baseline expression of L-selectin and CD11b on leukocytes from hemodialysis patients did not differ from that of healthy subjects. In CRF and hemodialysis patients, sL-selectin levels were significantly lower than in healthy subjects. During hemodialysis, cuprophane membrane induced an upregulation of Granulocyte CD11b, a decrease in Granulocyte L-selectin, and an increase in sL-selectin serum levels. Conversely, cellulose diacetate caused only a transient increase in Granulocyte CD11b and did not modify Granulocyte L-selectin and sL-selectin serum levels. High CD11b and low L-selectin expression on Granulocytes in CRF patients suggests an activation state, which was not found in hemodialysis patients at the basal state. The lack of activation in hemodialysis patients could reflect the elimination of a uremic toxin by dialysis or a loss of Granulocyte responsiveness because of the repetitive stimulation by hemodialysis treatment. The low serum levels of sL-selectin in CRF and hemodialysis patients also suggest Granulocyte dysfunction. (Am J Kidney Dis 1998 Jan;31(1):67-73)
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Effect of uremia and hemodialysis on soluble L-selectin and leukocyte surface CD11b and L-selectin
American Journal of Kidney Diseases, 1998Co-Authors: Laetitia Dou, Philippe Brunet, Françoise Dignat-george, José Sampol, Yvon BerlandAbstract:Leukocyte Mac-1 (CD11b/CD18) and L-selectin (CD62L) are implicated in leukocyte adhesion to endothelial cells. In this study, L-selectin and CD11b expression on leukocytes and soluble L-selectin (sL-selectin) serum levels were investigated in 17 nondialyzed patients with chronic renal failure (CRF), in 28 chronic hemodialysis patients before hemodialysis (basal state), and in 32 healthy subjects. These parameters were also monitored during hemodialysis with cuprophane and cellulose diacetate membranes in a crossover study in five patients. Granulocytes from CRF patients displayed lower expression of L-selectin and higher expression of CD11b than Granulocytes from healthy subjects. On the other hand, baseline expression of L-selectin and CD11b on leukocytes from hemodialysis patients did not differ from that of healthy subjects. In CRF and hemodialysis patients, sL-selectin levels were significantly lower than in healthy subjects. During hemodialysis, cuprophane membrane induced an upregulation of Granulocyte CD11b, a decrease in Granulocyte L-selectin, and an increase in sL-selectin serum levels. Conversely, cellulose diacetate caused only a transient increase in Granulocyte CD11b and did not modify Granulocyte L-selectin and sL-selectin serum levels. High CD11b and low L-selectin expression on Granulocytes in CRF patients suggests an activation state, which was not found in hemodialysis patients at the basal state. The lack of activation in hemodialysis patients could reflect the elimination of a uremic toxin by dialysis or a loss of Granulocyte responsiveness because of the repetitive stimulation by hemodialysis treatment. The low serum levels of sL-selectin in CRF and hemodialysis patients also suggest Granulocyte dysfunction. (C) 1998 by the National Kidney Foundation, Inc.
José Sampol - One of the best experts on this subject based on the ideXlab platform.
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Effect of uremia and hemodialysis on soluble L-selectin and leukocyte surface CD11b and L-selectin
American Journal of Kidney Diseases, 1998Co-Authors: Philippe Brunet, Françoise Dignat-george, José Sampol, Yvon BerlandAbstract:Abstract Leukocyte Mac-1 (CD11b/CD18) and L-selectin (CD62L) are implicated in leukocyte adhesion to endothelial cells. In this study, L-selectin and CD11b expression on leukocytes and soluble L-selectin (sL-selectin) serum levels were investigated in 17 nondialyzed patients with chronic renal failure (CRF), in 28 chronic hemodialysis patients before hemodialysis (basal state), and in 32 healthy subjects. These parameters were also monitored during hemodialysis with cuprophane and cellulose diacetate membranes in a crossover study in five patients. Granulocytes from CRF patients displayed lower expression of L-selectin and higher expression of CD11b than Granulocytes from healthy subjects. On the other hand, baseline expression of L-selectin and CD11b on leukocytes from hemodialysis patients did not differ from that of healthy subjects. In CRF and hemodialysis patients, sL-selectin levels were significantly lower than in healthy subjects. During hemodialysis, cuprophane membrane induced an upregulation of Granulocyte CD11b, a decrease in Granulocyte L-selectin, and an increase in sL-selectin serum levels. Conversely, cellulose diacetate caused only a transient increase in Granulocyte CD11b and did not modify Granulocyte L-selectin and sL-selectin serum levels. High CD11b and low L-selectin expression on Granulocytes in CRF patients suggests an activation state, which was not found in hemodialysis patients at the basal state. The lack of activation in hemodialysis patients could reflect the elimination of a uremic toxin by dialysis or a loss of Granulocyte responsiveness because of the repetitive stimulation by hemodialysis treatment. The low serum levels of sL-selectin in CRF and hemodialysis patients also suggest Granulocyte dysfunction. (Am J Kidney Dis 1998 Jan;31(1):67-73)
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Effect of uremia and hemodialysis on soluble L-selectin and leukocyte surface CD11b and L-selectin
American Journal of Kidney Diseases, 1998Co-Authors: Laetitia Dou, Philippe Brunet, Françoise Dignat-george, José Sampol, Yvon BerlandAbstract:Leukocyte Mac-1 (CD11b/CD18) and L-selectin (CD62L) are implicated in leukocyte adhesion to endothelial cells. In this study, L-selectin and CD11b expression on leukocytes and soluble L-selectin (sL-selectin) serum levels were investigated in 17 nondialyzed patients with chronic renal failure (CRF), in 28 chronic hemodialysis patients before hemodialysis (basal state), and in 32 healthy subjects. These parameters were also monitored during hemodialysis with cuprophane and cellulose diacetate membranes in a crossover study in five patients. Granulocytes from CRF patients displayed lower expression of L-selectin and higher expression of CD11b than Granulocytes from healthy subjects. On the other hand, baseline expression of L-selectin and CD11b on leukocytes from hemodialysis patients did not differ from that of healthy subjects. In CRF and hemodialysis patients, sL-selectin levels were significantly lower than in healthy subjects. During hemodialysis, cuprophane membrane induced an upregulation of Granulocyte CD11b, a decrease in Granulocyte L-selectin, and an increase in sL-selectin serum levels. Conversely, cellulose diacetate caused only a transient increase in Granulocyte CD11b and did not modify Granulocyte L-selectin and sL-selectin serum levels. High CD11b and low L-selectin expression on Granulocytes in CRF patients suggests an activation state, which was not found in hemodialysis patients at the basal state. The lack of activation in hemodialysis patients could reflect the elimination of a uremic toxin by dialysis or a loss of Granulocyte responsiveness because of the repetitive stimulation by hemodialysis treatment. The low serum levels of sL-selectin in CRF and hemodialysis patients also suggest Granulocyte dysfunction. (C) 1998 by the National Kidney Foundation, Inc.
