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Jorg Overmann - One of the best experts on this subject based on the ideXlab platform.
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Luteitalea pratensis gen. nov., sp. nov. a new member of subdivision 6 Acidobacteria isolated from temperate Grassland Soil.
International journal of systematic and evolutionary microbiology, 2017Co-Authors: Selma Vieira, Manja Luckner, Gerhard Wanner, Jorg OvermannAbstract:Albeit being widespread and abundant in Soils worldwide, bacteria of the phylum Acidobacteria have remained grossly understudied due to difficulties in their cultivation and isolation. To date, only 48 species have been validly described, including a single member of the phylogenetically diverse Acidobacteria subdivision 6. Here, we report the polyphasic characterization of strain HEG_-6_39T, a novel representative of Acidobacteria subdivision 6 isolated from a Grassland Soil in Thuringia, Germany. Cells of HEG_-6_39T are Gram-stain-negative, non-motile, non-spore-forming, non-capsulated short rods that form small dark yellow colonies. This slow growing bacterium is psychrotolerant and grows between 0 and 36 °C. It displays a narrower pH tolerance (5.3-8.3) than most acidobacteria. The strain is an aerobe that grows chemo-organotrophically utilizing mostly sugars and proteinaceous substrates such as peptone, yeast extract, casein hydrolysate and casamino acids as substrates. Diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylglycerol and two unknown phospholipids are identified as polar lipids. Major fatty acids are iso-C15 : 0, summed feature 3 (C16 : 1ω6c/C16 : 1ω7c), C18 : 1ω9c and iso-C17 : 1ω9c. The major respiratory quinone is MK-8. The G+C content of the genomic DNA is 64.7 mol%. 16S rRNA gene sequence analysis indicated that this bacterium was related to Vicinamibacter silvestris Ac_5_C6T with 93.6 % sequence similarity. Based on the present taxonomic characterization, strain HEG_-6_39T represents a new species of a novel genus for which the name Luteitalea pratensis gen. nov., sp. nov., is proposed. The type strain of the type species is HEG_-6_39T (=DSM 100886T=KCTC 52215T).
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luteitalea pratensis gen nov sp nov a new member of subdivision 6 acidobacteria isolated from temperate Grassland Soil
International Journal of Systematic and Evolutionary Microbiology, 2017Co-Authors: Selma Vieira, Jorg Overmann, Gerhard Wanner, Manja LucknerAbstract:Albeit being widespread and abundant in Soils worldwide, bacteria of the phylum Acidobacteria have remained grossly understudied due to difficulties in their cultivation and isolation. To date, only 48 species have been validly described, including a single member of the phylogenetically diverse Acidobacteria subdivision 6. Here, we report the polyphasic characterization of strain HEG_-6_39(T), a novel representative of Acidobacteria subdivision 6 isolated from a Grassland Soil in Thuringia, Germany. Cells of HEG_-6_39(T) are Gram-stain-negative, non-motile, non-spore-forming, non-capsulated short rods that form small dark yellow colonies. This slow growing bacterium is psychrotolerant and grows between 0 and 36 degrees C. It displays a narrower pH tolerance (5.3-8.3) than most acidobacteria. The strain is an aerobe that grows chemo-organotrophically utilizing mostly sugars and proteinaceous substrates such as peptone, yeast extract, casein hydrolysate and casamino acids as substrates. Diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylglycerol and two unknown phospholipids are identified as polar lipids. Major fatty acids are iso-C-15 : 0, summed feature 3 (C-16 : 1 omega 6c/C-16 : 1 omega 7c), C-18 : 1 omega 9c and iso-C-17 : 1 omega 9c. The major respiratory quinone is MK-8. The G+C content of the genomic DNA is 64.7 mol%. 16S rRNA gene sequence analysis indicated that this bacterium was related to Vicinamibacter silvestris Ac_5_C6(T) with 93.6% sequence similarity. Based on the present taxonomic characterization, strain HEG_-6_39(T) represents a new species of a novel genus for which the name Luteitalea pratensis gen. nov., sp. nov., is proposed. The type strain of the type species is HEG_6_39(T) (=DSM 100886(T)=KCTC 52215(T)).
Sarah J Kemmitt - One of the best experts on this subject based on the ideXlab platform.
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Soil acidification used as a management strategy to reduce nitrate losses from agricultural land
Soil Biology & Biochemistry, 2005Co-Authors: Sarah J Kemmitt, David L Wright, David L JonesAbstract:Abstract pH is known to be a primary regulator of nutrient cycling in Soil. Increasing Soil acidity in agricultural systems has the potential to slow down N cycling and reduce N losses from leaching thereby enhancing sustainability and reducing pollution. We conducted a field experiment to investigate the impact of acidity on N leaching in arable and Grassland agricultural systems. The results showed that nitrate (NO 3 − ) concentrations in Soil water were greater under arable than under Grassland. Soil acidification significantly lowered NO 3 − concentrations in Soil water over winter and spring under Grassland, whilst in cereal plots a similar effect was only observed in spring. Our results suggest that Soil acidification decreased nitrification causing an accumulation of NH 4 + which was not subject to leaching. Dissolved organic nitrogen (DON) concentrations in Soil water were significantly greater under arable than Grassland. Soil acidification lowered concentrations of DON in Soil water, usually to a greater extent in Grassland than in arable plots. It was concluded that it may be possible to use careful Soil pH management as a tool to control NO 3 − leaching without compromising the quality of drainage water, and that this may be more effective on Grassland than on arable crops.
Selma Vieira - One of the best experts on this subject based on the ideXlab platform.
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Luteitalea pratensis gen. nov., sp. nov. a new member of subdivision 6 Acidobacteria isolated from temperate Grassland Soil.
International journal of systematic and evolutionary microbiology, 2017Co-Authors: Selma Vieira, Manja Luckner, Gerhard Wanner, Jorg OvermannAbstract:Albeit being widespread and abundant in Soils worldwide, bacteria of the phylum Acidobacteria have remained grossly understudied due to difficulties in their cultivation and isolation. To date, only 48 species have been validly described, including a single member of the phylogenetically diverse Acidobacteria subdivision 6. Here, we report the polyphasic characterization of strain HEG_-6_39T, a novel representative of Acidobacteria subdivision 6 isolated from a Grassland Soil in Thuringia, Germany. Cells of HEG_-6_39T are Gram-stain-negative, non-motile, non-spore-forming, non-capsulated short rods that form small dark yellow colonies. This slow growing bacterium is psychrotolerant and grows between 0 and 36 °C. It displays a narrower pH tolerance (5.3-8.3) than most acidobacteria. The strain is an aerobe that grows chemo-organotrophically utilizing mostly sugars and proteinaceous substrates such as peptone, yeast extract, casein hydrolysate and casamino acids as substrates. Diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylglycerol and two unknown phospholipids are identified as polar lipids. Major fatty acids are iso-C15 : 0, summed feature 3 (C16 : 1ω6c/C16 : 1ω7c), C18 : 1ω9c and iso-C17 : 1ω9c. The major respiratory quinone is MK-8. The G+C content of the genomic DNA is 64.7 mol%. 16S rRNA gene sequence analysis indicated that this bacterium was related to Vicinamibacter silvestris Ac_5_C6T with 93.6 % sequence similarity. Based on the present taxonomic characterization, strain HEG_-6_39T represents a new species of a novel genus for which the name Luteitalea pratensis gen. nov., sp. nov., is proposed. The type strain of the type species is HEG_-6_39T (=DSM 100886T=KCTC 52215T).
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luteitalea pratensis gen nov sp nov a new member of subdivision 6 acidobacteria isolated from temperate Grassland Soil
International Journal of Systematic and Evolutionary Microbiology, 2017Co-Authors: Selma Vieira, Jorg Overmann, Gerhard Wanner, Manja LucknerAbstract:Albeit being widespread and abundant in Soils worldwide, bacteria of the phylum Acidobacteria have remained grossly understudied due to difficulties in their cultivation and isolation. To date, only 48 species have been validly described, including a single member of the phylogenetically diverse Acidobacteria subdivision 6. Here, we report the polyphasic characterization of strain HEG_-6_39(T), a novel representative of Acidobacteria subdivision 6 isolated from a Grassland Soil in Thuringia, Germany. Cells of HEG_-6_39(T) are Gram-stain-negative, non-motile, non-spore-forming, non-capsulated short rods that form small dark yellow colonies. This slow growing bacterium is psychrotolerant and grows between 0 and 36 degrees C. It displays a narrower pH tolerance (5.3-8.3) than most acidobacteria. The strain is an aerobe that grows chemo-organotrophically utilizing mostly sugars and proteinaceous substrates such as peptone, yeast extract, casein hydrolysate and casamino acids as substrates. Diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylglycerol and two unknown phospholipids are identified as polar lipids. Major fatty acids are iso-C-15 : 0, summed feature 3 (C-16 : 1 omega 6c/C-16 : 1 omega 7c), C-18 : 1 omega 9c and iso-C-17 : 1 omega 9c. The major respiratory quinone is MK-8. The G+C content of the genomic DNA is 64.7 mol%. 16S rRNA gene sequence analysis indicated that this bacterium was related to Vicinamibacter silvestris Ac_5_C6(T) with 93.6% sequence similarity. Based on the present taxonomic characterization, strain HEG_-6_39(T) represents a new species of a novel genus for which the name Luteitalea pratensis gen. nov., sp. nov., is proposed. The type strain of the type species is HEG_6_39(T) (=DSM 100886(T)=KCTC 52215(T)).
Penny R. Hirsch - One of the best experts on this subject based on the ideXlab platform.
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Structure, fluctuation and magnitude of a natural Grassland Soil metagenome
The ISME Journal, 2012Co-Authors: Tom O. Delmont, Michael Faubladier, Kevin Patrick Keegan, Patrick Robe, Penny R. Hirsch, Folker Meyer, Emmanuel Prestat, Ian M. Clark, Eric Pelletier, Jack A. GilbertAbstract:The Soil ecosystem is critical for human health, affecting aspects of the environment from key agricultural and edaphic parameters to critical influence on climate change. Soil has more unknown biodiversity than any other ecosystem. We have applied diverse DNA extraction methods coupled with high throughput pyrosequencing to explore 4.88 × 109 bp of metagenomic sequence data from the longest continually studied Soil environment (Park Grass experiment at Rothamsted Research in the UK). Results emphasize important DNA extraction biases and unexpectedly low seasonal and vertical Soil metagenomic functional class variations. Clustering-based subsystems and carbohydrate metabolism had the largest quantity of annotated reads assigned although
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structure fluctuation and magnitude of a natural Grassland Soil metagenome
The ISME Journal, 2012Co-Authors: Tom O. Delmont, Michael Faubladier, Kevin Patrick Keegan, Patrick Robe, Emmanuel Prestat, Eric Pelletier, Ian Clark, Penny R. HirschAbstract:The Soil ecosystem is critical for human health, affecting aspects of the environment from key agricultural and edaphic parameters to critical influence on climate change. Soil has more unknown biodiversity than any other ecosystem. We have applied diverse DNA extraction methods coupled with high throughput pyrosequencing to explore 4.88 × 10(9) bp of metagenomic sequence data from the longest continually studied Soil environment (Park Grass experiment at Rothamsted Research in the UK). Results emphasize important DNA extraction biases and unexpectedly low seasonal and vertical Soil metagenomic functional class variations. Clustering-based subsystems and carbohydrate metabolism had the largest quantity of annotated reads assigned although <50% of reads were assigned at an E value cutoff of 10(-5). In addition, with the more detailed subsystems, cAMP signaling in bacteria (3.24±0.27% of the annotated reads) and the Ton and Tol transport systems (1.69±0.11%) were relatively highly represented. The most highly represented genome from the database was that for a Bradyrhizobium species. The metagenomic variance created by integrating natural and methodological fluctuations represents a global picture of the Rothamsted Soil metagenome that can be used for specific questions and future inter-environmental metagenomic comparisons. However, only 1% of annotated sequences correspond to already sequenced genomes at 96% similarity and E values of <10(-5), thus, considerable genomic reconstructions efforts still have to be performed.
Eric Pelletier - One of the best experts on this subject based on the ideXlab platform.
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Structure, fluctuation and magnitude of a natural Grassland Soil metagenome
The ISME Journal, 2012Co-Authors: Tom O. Delmont, Michael Faubladier, Kevin Patrick Keegan, Patrick Robe, Penny R. Hirsch, Folker Meyer, Emmanuel Prestat, Ian M. Clark, Eric Pelletier, Jack A. GilbertAbstract:The Soil ecosystem is critical for human health, affecting aspects of the environment from key agricultural and edaphic parameters to critical influence on climate change. Soil has more unknown biodiversity than any other ecosystem. We have applied diverse DNA extraction methods coupled with high throughput pyrosequencing to explore 4.88 × 109 bp of metagenomic sequence data from the longest continually studied Soil environment (Park Grass experiment at Rothamsted Research in the UK). Results emphasize important DNA extraction biases and unexpectedly low seasonal and vertical Soil metagenomic functional class variations. Clustering-based subsystems and carbohydrate metabolism had the largest quantity of annotated reads assigned although
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structure fluctuation and magnitude of a natural Grassland Soil metagenome
The ISME Journal, 2012Co-Authors: Tom O. Delmont, Michael Faubladier, Kevin Patrick Keegan, Patrick Robe, Emmanuel Prestat, Eric Pelletier, Ian Clark, Penny R. HirschAbstract:The Soil ecosystem is critical for human health, affecting aspects of the environment from key agricultural and edaphic parameters to critical influence on climate change. Soil has more unknown biodiversity than any other ecosystem. We have applied diverse DNA extraction methods coupled with high throughput pyrosequencing to explore 4.88 × 10(9) bp of metagenomic sequence data from the longest continually studied Soil environment (Park Grass experiment at Rothamsted Research in the UK). Results emphasize important DNA extraction biases and unexpectedly low seasonal and vertical Soil metagenomic functional class variations. Clustering-based subsystems and carbohydrate metabolism had the largest quantity of annotated reads assigned although <50% of reads were assigned at an E value cutoff of 10(-5). In addition, with the more detailed subsystems, cAMP signaling in bacteria (3.24±0.27% of the annotated reads) and the Ton and Tol transport systems (1.69±0.11%) were relatively highly represented. The most highly represented genome from the database was that for a Bradyrhizobium species. The metagenomic variance created by integrating natural and methodological fluctuations represents a global picture of the Rothamsted Soil metagenome that can be used for specific questions and future inter-environmental metagenomic comparisons. However, only 1% of annotated sequences correspond to already sequenced genomes at 96% similarity and E values of <10(-5), thus, considerable genomic reconstructions efforts still have to be performed.
