The Experts below are selected from a list of 33 Experts worldwide ranked by ideXlab platform
Raj Kumar Singh - One of the best experts on this subject based on the ideXlab platform.
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Detection of bluetongue virus Group-Specific Antigen using monoclonal antibody based sandwich ELISA
Virologica Sinica, 2010Co-Authors: Pradeep Narayan Gandhale, Veerakyathappa Bhanuprakash, Vinayagamurthy Balamurugan, Madhusudhan Hosamani, Gnanavel Venkatesan, Raj Kumar SinghAbstract:A monoclonal antibody (MAb) specific for the bluetongue virus (BTV) group specific Antigen (VP7) was characterized for its reactivity with purified virus and recombinant BTV VP7 (rVP7) protein and its suitability for use in the sandwich ELISA. The MAb, designated as 5B5 was specific to VP7 and belongs to IgG2a subclass and was selected for the development of the sELISA in this study. The MAb had a titer of 1:25 with BTV and 1:2 with the rVP7 protein. The sELISA is based on capturing of BTV Antigen with VP7 specific MAb followed by detection using BTV polyclonal antiserum raised in rabbits. The assay was evaluated with six cell culture adapted serotypes of BTV that have been isolated from India, 1, 2, 15, 17, 18 and 23. The assay could detect BTV Antigen as early as day 8 in blood. It was also successfully applied for the detection of BTV group specific Antigen in clinical samples of blood, washed RBCs, buffy coat and plasma. A total of 102 field samples from animals, suspected of being infected with BTV, were tested and 29.42% were positive. The blood samples were also amplified in cell culture which improved the sensitivity of the assay. Results confirmed that the sELISA is rapid and specific.
Pradeep Narayan Gandhale - One of the best experts on this subject based on the ideXlab platform.
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Detection of bluetongue virus Group-Specific Antigen using monoclonal antibody based sandwich ELISA
Virologica Sinica, 2010Co-Authors: Pradeep Narayan Gandhale, Veerakyathappa Bhanuprakash, Vinayagamurthy Balamurugan, Madhusudhan Hosamani, Gnanavel Venkatesan, Raj Kumar SinghAbstract:A monoclonal antibody (MAb) specific for the bluetongue virus (BTV) group specific Antigen (VP7) was characterized for its reactivity with purified virus and recombinant BTV VP7 (rVP7) protein and its suitability for use in the sandwich ELISA. The MAb, designated as 5B5 was specific to VP7 and belongs to IgG2a subclass and was selected for the development of the sELISA in this study. The MAb had a titer of 1:25 with BTV and 1:2 with the rVP7 protein. The sELISA is based on capturing of BTV Antigen with VP7 specific MAb followed by detection using BTV polyclonal antiserum raised in rabbits. The assay was evaluated with six cell culture adapted serotypes of BTV that have been isolated from India, 1, 2, 15, 17, 18 and 23. The assay could detect BTV Antigen as early as day 8 in blood. It was also successfully applied for the detection of BTV group specific Antigen in clinical samples of blood, washed RBCs, buffy coat and plasma. A total of 102 field samples from animals, suspected of being infected with BTV, were tested and 29.42% were positive. The blood samples were also amplified in cell culture which improved the sensitivity of the assay. Results confirmed that the sELISA is rapid and specific.
Veerakyathappa Bhanuprakash - One of the best experts on this subject based on the ideXlab platform.
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Detection of bluetongue virus Group-Specific Antigen using monoclonal antibody based sandwich ELISA
Virologica Sinica, 2010Co-Authors: Pradeep Narayan Gandhale, Veerakyathappa Bhanuprakash, Vinayagamurthy Balamurugan, Madhusudhan Hosamani, Gnanavel Venkatesan, Raj Kumar SinghAbstract:A monoclonal antibody (MAb) specific for the bluetongue virus (BTV) group specific Antigen (VP7) was characterized for its reactivity with purified virus and recombinant BTV VP7 (rVP7) protein and its suitability for use in the sandwich ELISA. The MAb, designated as 5B5 was specific to VP7 and belongs to IgG2a subclass and was selected for the development of the sELISA in this study. The MAb had a titer of 1:25 with BTV and 1:2 with the rVP7 protein. The sELISA is based on capturing of BTV Antigen with VP7 specific MAb followed by detection using BTV polyclonal antiserum raised in rabbits. The assay was evaluated with six cell culture adapted serotypes of BTV that have been isolated from India, 1, 2, 15, 17, 18 and 23. The assay could detect BTV Antigen as early as day 8 in blood. It was also successfully applied for the detection of BTV group specific Antigen in clinical samples of blood, washed RBCs, buffy coat and plasma. A total of 102 field samples from animals, suspected of being infected with BTV, were tested and 29.42% were positive. The blood samples were also amplified in cell culture which improved the sensitivity of the assay. Results confirmed that the sELISA is rapid and specific.
Vinayagamurthy Balamurugan - One of the best experts on this subject based on the ideXlab platform.
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Detection of bluetongue virus Group-Specific Antigen using monoclonal antibody based sandwich ELISA
Virologica Sinica, 2010Co-Authors: Pradeep Narayan Gandhale, Veerakyathappa Bhanuprakash, Vinayagamurthy Balamurugan, Madhusudhan Hosamani, Gnanavel Venkatesan, Raj Kumar SinghAbstract:A monoclonal antibody (MAb) specific for the bluetongue virus (BTV) group specific Antigen (VP7) was characterized for its reactivity with purified virus and recombinant BTV VP7 (rVP7) protein and its suitability for use in the sandwich ELISA. The MAb, designated as 5B5 was specific to VP7 and belongs to IgG2a subclass and was selected for the development of the sELISA in this study. The MAb had a titer of 1:25 with BTV and 1:2 with the rVP7 protein. The sELISA is based on capturing of BTV Antigen with VP7 specific MAb followed by detection using BTV polyclonal antiserum raised in rabbits. The assay was evaluated with six cell culture adapted serotypes of BTV that have been isolated from India, 1, 2, 15, 17, 18 and 23. The assay could detect BTV Antigen as early as day 8 in blood. It was also successfully applied for the detection of BTV group specific Antigen in clinical samples of blood, washed RBCs, buffy coat and plasma. A total of 102 field samples from animals, suspected of being infected with BTV, were tested and 29.42% were positive. The blood samples were also amplified in cell culture which improved the sensitivity of the assay. Results confirmed that the sELISA is rapid and specific.
Madhusudhan Hosamani - One of the best experts on this subject based on the ideXlab platform.
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Detection of bluetongue virus Group-Specific Antigen using monoclonal antibody based sandwich ELISA
Virologica Sinica, 2010Co-Authors: Pradeep Narayan Gandhale, Veerakyathappa Bhanuprakash, Vinayagamurthy Balamurugan, Madhusudhan Hosamani, Gnanavel Venkatesan, Raj Kumar SinghAbstract:A monoclonal antibody (MAb) specific for the bluetongue virus (BTV) group specific Antigen (VP7) was characterized for its reactivity with purified virus and recombinant BTV VP7 (rVP7) protein and its suitability for use in the sandwich ELISA. The MAb, designated as 5B5 was specific to VP7 and belongs to IgG2a subclass and was selected for the development of the sELISA in this study. The MAb had a titer of 1:25 with BTV and 1:2 with the rVP7 protein. The sELISA is based on capturing of BTV Antigen with VP7 specific MAb followed by detection using BTV polyclonal antiserum raised in rabbits. The assay was evaluated with six cell culture adapted serotypes of BTV that have been isolated from India, 1, 2, 15, 17, 18 and 23. The assay could detect BTV Antigen as early as day 8 in blood. It was also successfully applied for the detection of BTV group specific Antigen in clinical samples of blood, washed RBCs, buffy coat and plasma. A total of 102 field samples from animals, suspected of being infected with BTV, were tested and 29.42% were positive. The blood samples were also amplified in cell culture which improved the sensitivity of the assay. Results confirmed that the sELISA is rapid and specific.
