The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Wanchai Maleewong - One of the best experts on this subject based on the ideXlab platform.
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Growth and Development of gnathostoma spinigerum nematoda gnathostomatidae larvae in mesocyclops aspericornis cyclopoida cyclopidae
Parasites & Vectors, 2011Co-Authors: Penchom Janwan, Luxkhana Sadaow, Oranuch Sanpool, Tongjit Thanchomnang, Wanchai MaleewongAbstract:Background Gnathostoma spinigerum larva is pathogenic, causing gnathostomiasis in humans and certain animals, and is prevalent mainly in Asia. Growth and Development of Gnathostoma spinigerum larvae in the cyclopoid copepod Mesocyclops aspericornis, the first intermediate host, were examined.
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Growth and Development of gnathostoma spinigerum early third stage larvae in vitro
Journal of Helminthology, 1997Co-Authors: Wanchai Maleewong, Kosol Ieamviteevanich, Chaisiri WongkhamAbstract:Early third-stage larvae of Gnathostoma spinigerum were cultured in RPMI-1640 supplemented with 25mM NaHCO 3 ,100 units/ml penicillin G, 100 μg/ml of streptomycin, 5 μg/ml of amphotericin B and 10% foetal calf serum at 37°C under 5% CO 2 in air for 60 days. After 3 days of cultivation, the larvae moulted. Body sizes increased from 0.49 ± 0.09 × 0.07 ± 0.01 mm in length and width to 4.08 ± 0.48 × 0.32 ± 0.04 mm after 60 days of cultivation. The maximum body length and width of these larvae were 4.94 mm and 0.35 mm, respectively. The survival rate (67.5 %) of the worms was observed at the end of cultivation. The addition of foetal calf serum was found to be essential for Growth and Development.
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gnathostoma spinigerum Growth and Development of third stage larvae in vitro
Journal of Parasitology, 1995Co-Authors: Wanchai Maleewong, Jeerapa Khempila, Suwin Wongwajana, Chaisiri WongkhamAbstract:Advanced third-stage larvae of Gnathostoma spinigerum were cultured in RPMI-1640, with various supplements at 37 C under 5% CO2 in air for 300 days. The most suitable medium supplement for worm Development was 10% fetal calf serum, 1% dog serum, and 0.25% dog hemolysate. After approximately 180 days of cultivation, some larvae molted to the fourth stage as distinguished by 8 transverse rows of cephalic hooklets and well differentiated sex organs. The maximum body length and width of these larvae were 18.6 mm and 1.1 mm, respectively. Six of 50 larvae (12%) developed to the fourth stage, with a 32% survival rate at the end of cultivation. Although the highest survival rate (70%) of the worms was observed in the medium supplemented with 25 mM NaHCO 3 , only 4% developed into fourth stage larvae. The addition of fetal calf serum, dog serum, and dog hemolysate was essential for Growth and Development.
Gabor Galiba - One of the best experts on this subject based on the ideXlab platform.
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redox control of plant Growth and Development
Plant Science, 2013Co-Authors: Gabor Kocsy, Irma Tari, Radomira Vankova, Bernd Zechmann, Zsolt Gulyas, Peter Poor, Gabor GalibaAbstract:Redox changes determined by genetic and environmental factors display well-organized interactions in the control of plant Growth and Development. Diurnal and seasonal changes in the environmental conditions are important for the normal course of these physiological processes and, similarly to their mild irregular alterations, for stress adaptation. However, fast or large-scale environmental changes may lead to damage or death of sensitive plants. The spatial and temporal redox changes influence Growth and Development due to the reprogramming of metabolism. In this process reactive oxygen and nitrogen species and antioxidants are involved as components of signalling networks. The control of Growth, Development and flowering by reactive oxygen and nitrogen species and antioxidants in interaction with hormones at organ, tissue, cellular and subcellular level will be discussed in the present review. Unsolved problems of the field, among others the need for identification of new components and interactions in the redox regulatory network at various organization levels using systems biology approaches will be also indicated.
J. P. Sumpter - One of the best experts on this subject based on the ideXlab platform.
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Oocyte Growth and Development in teleosts
Reviews in Fish Biology and Fisheries, 1996Co-Authors: C. R. Tyler, J. P. SumpterAbstract:Oocyte Growth and Development is an important issue in fish and fisheries biology. This paper reviews the information available on oocyte Growth patterns and the rates and dynamics of oocyte Growth in teleosts. In synchronous spawners, the weight of the gonad may represent as much as 40% of the overall body weight of the fish. In asynchronous spawners, the weight of the mature ovary is considerably less than in synchronous ovulators, but the ovary shows a more regular periodicity and may grow repeatedly many times during the breeding season. There is a huge variability in egg size in teleosts, with the largest known measuring up to 8 cm in diameter. Within the limits of variance set by genetic constraints, egg size may vary between populations of the same species. Oocytes in all teleosts undergo the same basic pattern of Growth: oogenesis, primary oocyte Growth, cortical alveolus stage, vitellogenesis, maturation and ovulation. The mechanisms that control oocyte Growth are addressed in this review, albeit that the available information, as in all other vertebrates, is very limited. The main hormones that have been shown to affect ovarian Growth are gonadotrophin, thyroid hormones, Growth hormone, insulin and insulin-like Growth factors. An overview of the determinants of fecundity, with particular reference to oocyte recruitment and atresia, is the focus of the second part of the paper. Genetics and nutrition have major effects on fecundity, and studies so far suggest that the determinants of fecundity usually operate during the early part of gametogenesis. The role of atresia in determining fecundity is less clear. The final part of this review highlights some areas of study that are priorities for research on ovarian Development in fish.
Pamela Hunt - One of the best experts on this subject based on the ideXlab platform.
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RAPID COMMUNICATION Recombinant Human Megakaryocyte Growth and Development Factor
2013Co-Authors: Stimulates Thrombocytopoiesis, Normal Nonhuman Primates, M. Farese, Pamela Hunt, Thomas Boone, Thomas J. MacvittieAbstract:Megakaryocyte Growth and Development factor (MGDF) is a novel cytokine that binds to the c-mpl receptor and stimulates megakaryocyte Development in vitro and in vivo. This report describes the ability of recombinant human (r-Hu) MGDF to affect megakaryocytopoiesis in normal nonhuman primates. r-HuMGDF was administered subcutaneously to normal, male rhesus monkeys once per day for 10 consecutive days at dosages of 2.5,25, or 250 pglkg of body weight. Bone marrow and peripheral blood were assayed for clono-genic activity and peripheral blood counts were monitored. Circulating platelet counts increased significantly (P<,051 for all doses within 6 days of r-HuMGDF administration and reached maximal levels between day 12 and day 14 postcy-tokine administration. The 2.5, 25.0, and 250.0 pglkgld doses elicited peak mean platelet counts that were 592%
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the role of megakaryocyte Growth and Development factor in terminal stages of thrombopoiesis
British Journal of Haematology, 1996Co-Authors: Esther S Choi, Janet L Nichol, Martha M Hokom, Jenli Chen, James D Skrine, Judy Faust, Pamela HuntAbstract:Thrombopoietin (TPO), the ligand for the c-Mpl cytokine receptor, is a recently identified cytokine with potent effects on platelet production. The receptor-binding portion of c-Mpl ligand is encompassed in another molecule known as megakaryocyte Growth and Development factor, or MGDF. Although it is clear that the administration of TPO or MGDF to animals dramatically increases the platelet count, the specific stage(s) of thrombopoiesis during which these molecules are principally active have not been unambiguously determined. Pharmacology studies administering MGDF at doses ranging from 0.1 to 630 micrograms/kg/d to mice revealed a biphasic response in platelet production. Administration of the drug at concentrations from 6 to 60 micrograms/kg/d resulted in platelet counts 5-fold above normal. However, doses > 60 micrograms/kg/d resulted in less-than-optimal platelet production. This phenomenon was investigated in vitro. Using an established culture system for the generation of human megakaryocytes and platelets, MGDF was shown to be optimally and equivalently active in the generation of mature megakaryocytes at concentrations from 10 to 1000 ng/ml. However, the cytokine was not required for proplatelet formation and in fact was inhibitory to that process in a dose-dependent manner. When MGDF was added to human megakaryocytes at concentrations of 200 ng/ml or greater, proplatelet formation was inhibited to 30% of control values. MGDF-mediated inhibition was specific, since the addition of the truncated form of the c-Mpl receptor reversed the inhibition in a dose-dependent manner. Other recombinant factors, interleukin-6, interleukin-11 and erythropoietin had no significant positive or negative effects in this human proplatelet assay. Together, these data suggest that although TPO and MGDF promote the full spectrum of megakaryocyte Growth and Development, they are not necessary for proplatelet formation, and may in part regulate platelet shedding by their absence.
Tongjit Thanchomnang - One of the best experts on this subject based on the ideXlab platform.
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Growth and Development of gnathostoma spinigerum nematoda gnathostomatidae larvae in mesocyclops aspericornis cyclopoida cyclopidae
Parasites & Vectors, 2011Co-Authors: Penchom Janwan, Luxkhana Sadaow, Oranuch Sanpool, Tongjit Thanchomnang, Wanchai MaleewongAbstract:Background Gnathostoma spinigerum larva is pathogenic, causing gnathostomiasis in humans and certain animals, and is prevalent mainly in Asia. Growth and Development of Gnathostoma spinigerum larvae in the cyclopoid copepod Mesocyclops aspericornis, the first intermediate host, were examined.
