The Experts below are selected from a list of 231 Experts worldwide ranked by ideXlab platform
Nicoletta Sacchi - One of the best experts on this subject based on the ideXlab platform.
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the single step method of rna isolation by acid Guanidinium Thiocyanate phenol chloroform extraction twenty something years on
Nature Protocols, 2006Co-Authors: Piotr Chomczynski, Nicoletta SacchiAbstract:The single-step method of RNA isolation by acid Guanidinium Thiocyanate–phenol–chloroform extraction: twenty-something years on
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the single step method of rna isolation by acid Guanidinium Thiocyanate phenol chloroform extraction twenty something years on
Nature Protocols, 2006Co-Authors: Piotr Chomczynski, Nicoletta SacchiAbstract:Since its introduction, the 'single-step' method has become widely used for isolating total RNA from biological samples of different sources. The principle at the basis of the method is that RNA is separated from DNA after extraction with an acidic solution containing Guanidinium Thiocyanate, sodium acetate, phenol and chloroform, followed by centrifugation. Under acidic conditions, total RNA remains in the upper aqueous phase, while most of DNA and proteins remain either in the interphase or in the lower organic phase. Total RNA is then recovered by precipitation with isopropanol and can be used for several applications. The original protocol, enabling the isolation of RNA from cells and tissues in less than 4 hours, greatly advanced the analysis of gene expression in plant and animal models as well as in pathological samples, as demonstrated by the overwhelming number of citations the paper gained over 20 years.
Piotr Chomczynski - One of the best experts on this subject based on the ideXlab platform.
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the single step method of rna isolation by acid Guanidinium Thiocyanate phenol chloroform extraction twenty something years on
Nature Protocols, 2006Co-Authors: Piotr Chomczynski, Nicoletta SacchiAbstract:The single-step method of RNA isolation by acid Guanidinium Thiocyanate–phenol–chloroform extraction: twenty-something years on
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the single step method of rna isolation by acid Guanidinium Thiocyanate phenol chloroform extraction twenty something years on
Nature Protocols, 2006Co-Authors: Piotr Chomczynski, Nicoletta SacchiAbstract:Since its introduction, the 'single-step' method has become widely used for isolating total RNA from biological samples of different sources. The principle at the basis of the method is that RNA is separated from DNA after extraction with an acidic solution containing Guanidinium Thiocyanate, sodium acetate, phenol and chloroform, followed by centrifugation. Under acidic conditions, total RNA remains in the upper aqueous phase, while most of DNA and proteins remain either in the interphase or in the lower organic phase. Total RNA is then recovered by precipitation with isopropanol and can be used for several applications. The original protocol, enabling the isolation of RNA from cells and tissues in less than 4 hours, greatly advanced the analysis of gene expression in plant and animal models as well as in pathological samples, as demonstrated by the overwhelming number of citations the paper gained over 20 years.
Dirk M. Guldi - One of the best experts on this subject based on the ideXlab platform.
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iodine pseudohalogen ionic liquid based electrolytes for quasi solid state dye sensitized solar cells
ACS Applied Materials & Interfaces, 2017Co-Authors: Annkatrin Lennert, Michelle Sternberg, Rubén D. Costa, Karsten Meyer, Dirk M. GuldiAbstract:In the current work, novel symmetrically alkyl-substituted imidazolium-based ionic liquids have been synthesized featuring either iodide (I–) or selenocyanate (SeCN–) as counteranions. Physicochemical assays based on spectroscopy and electrochemistry techniques have been performed to identify the best ionic liquid for application as electrolytes in quasi-solid-state dye-sensitized solar cells (qssDSSC). The latter were mixed with additives such as 4-tert-butylpyridine (4tbpy) and Guanidinium Thiocyanate (GuSCN) to optimize electrode surface coverage, ionic diffusion, and dye regeneration. In addition, we demonstrate that electrolytes containing a mixture of I2 and (SeCN)2 enhance the open-circuit voltage of the final quasi-solid-state device by up to 70 mV. As such, iodine-pseudohalogen electrolytes reveal in qssDSSCs a good balance between dye regeneration and hole transport and, in turn, enhance the overall solar energy conversion efficiency by 70% with respect to reference qssDSSCs with iodine-based el...
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Iodine-Pseudohalogen Ionic Liquid-Based Electrolytes for Quasi-Solid-State Dye-Sensitized Solar Cells
2017Co-Authors: Annkatrin Lennert, Michelle Sternberg, Rubén D. Costa, Karsten Meyer, Dirk M. GuldiAbstract:In the current work, novel symmetrically alkyl-substituted imidazolium-based ionic liquids have been synthesized featuring either iodide (I–) or selenocyanate (SeCN–) as counteranions. Physicochemical assays based on spectroscopy and electrochemistry techniques have been performed to identify the best ionic liquid for application as electrolytes in quasi-solid-state dye-sensitized solar cells (qssDSSC). The latter were mixed with additives such as 4-tert-butylpyridine (4tbpy) and Guanidinium Thiocyanate (GuSCN) to optimize electrode surface coverage, ionic diffusion, and dye regeneration. In addition, we demonstrate that electrolytes containing a mixture of I2 and (SeCN)2 enhance the open-circuit voltage of the final quasi-solid-state device by up to 70 mV. As such, iodine-pseudohalogen electrolytes reveal in qssDSSCs a good balance between dye regeneration and hole transport and, in turn, enhance the overall solar energy conversion efficiency by 70% with respect to reference qssDSSCs with iodine-based electrolytes. Finally, devices with the iodine-pseudohalogen electrolyte show a 1000 h stable efficiency of 7–8% under outdoor temperature operation conditions and 1 sun illumination
Yuan Lin - One of the best experts on this subject based on the ideXlab platform.
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nh 2 rich silica nanoparticle as a universal additive in electrolytes for high efficiency quasi solid state dye sensitized solar cells and quantum dot sensitized solar cells
Electrochimica Acta, 2018Co-Authors: Yanyan Fang, Hongbo Cheng, Yanan Wang, Xiaowen Zhou, Shibi Fang, Yuan LinAbstract:Abstract In this paper, a novel kind of functional NH2-rich silica nanoparticle (A-SiO2) as an electrolyte additive is reported, which is employed to assemble high-efficiency quasi-solid-state dye-sensitized solar cells (DSCs) and quantum dot sensitized solar cells (QDSCs), while the additional solidifying character of A-SiO2 makes it superior to the common additives. It is found that the A-SiO2 nanoparticle as an additive for ionic-liquid electrolyte can significantly improve the photovoltaic performance of quasi-solid-state DSCs, especially the open-circuit photovoltage (Voc) and fill factor (FF) through (1) negatively shifting the TiO2 conduction band (CB) edge, (2) effectively facilitating the ions transport and (3) remarkably inhibiting the charge recombination. Notably, DSC fabricated using the A-SiO2 based ionic-liquid gel electrolytes achieves a power conversion efficiency (PCE) of 7.30% under 1 sun illumination (AM 1.5 G, 100 mW cm−2), which is higher than that of DSC with the ionic-liquid electrolyte employing N-methylbenzimidazole (NMBI) and Guanidinium Thiocyanate (GuNCS) as additives (PCE = 6.23%). Moreover, the A-SiO2 additive is of the universality in organic electrolytes for DSCs and polysulfide electrolytes for QDSCs. The PCE of CdS/CdSe co-sensitized QDSCs using A-SiO2 additives is improved by 34.9% due to the enhancement of short-circuit current density (Jsc) and Voc, resulting in a champion PCE of 7.11%, which is one of the best results for CdS/CdSe co-sensitized QDSCs.
Annkatrin Lennert - One of the best experts on this subject based on the ideXlab platform.
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iodine pseudohalogen ionic liquid based electrolytes for quasi solid state dye sensitized solar cells
ACS Applied Materials & Interfaces, 2017Co-Authors: Annkatrin Lennert, Michelle Sternberg, Rubén D. Costa, Karsten Meyer, Dirk M. GuldiAbstract:In the current work, novel symmetrically alkyl-substituted imidazolium-based ionic liquids have been synthesized featuring either iodide (I–) or selenocyanate (SeCN–) as counteranions. Physicochemical assays based on spectroscopy and electrochemistry techniques have been performed to identify the best ionic liquid for application as electrolytes in quasi-solid-state dye-sensitized solar cells (qssDSSC). The latter were mixed with additives such as 4-tert-butylpyridine (4tbpy) and Guanidinium Thiocyanate (GuSCN) to optimize electrode surface coverage, ionic diffusion, and dye regeneration. In addition, we demonstrate that electrolytes containing a mixture of I2 and (SeCN)2 enhance the open-circuit voltage of the final quasi-solid-state device by up to 70 mV. As such, iodine-pseudohalogen electrolytes reveal in qssDSSCs a good balance between dye regeneration and hole transport and, in turn, enhance the overall solar energy conversion efficiency by 70% with respect to reference qssDSSCs with iodine-based el...
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Iodine-Pseudohalogen Ionic Liquid-Based Electrolytes for Quasi-Solid-State Dye-Sensitized Solar Cells
2017Co-Authors: Annkatrin Lennert, Michelle Sternberg, Rubén D. Costa, Karsten Meyer, Dirk M. GuldiAbstract:In the current work, novel symmetrically alkyl-substituted imidazolium-based ionic liquids have been synthesized featuring either iodide (I–) or selenocyanate (SeCN–) as counteranions. Physicochemical assays based on spectroscopy and electrochemistry techniques have been performed to identify the best ionic liquid for application as electrolytes in quasi-solid-state dye-sensitized solar cells (qssDSSC). The latter were mixed with additives such as 4-tert-butylpyridine (4tbpy) and Guanidinium Thiocyanate (GuSCN) to optimize electrode surface coverage, ionic diffusion, and dye regeneration. In addition, we demonstrate that electrolytes containing a mixture of I2 and (SeCN)2 enhance the open-circuit voltage of the final quasi-solid-state device by up to 70 mV. As such, iodine-pseudohalogen electrolytes reveal in qssDSSCs a good balance between dye regeneration and hole transport and, in turn, enhance the overall solar energy conversion efficiency by 70% with respect to reference qssDSSCs with iodine-based electrolytes. Finally, devices with the iodine-pseudohalogen electrolyte show a 1000 h stable efficiency of 7–8% under outdoor temperature operation conditions and 1 sun illumination
