The Experts below are selected from a list of 81 Experts worldwide ranked by ideXlab platform

Ian Kitchen - One of the best experts on this subject based on the ideXlab platform.

  • G-protein coupling of delta-opioid receptors in brains of mu-opioid receptor knockout mice.
    European journal of pharmacology, 2003
    Co-Authors: Sarah M. Oakley, Anna Borsodi, Brigitte L Kieffer, Géza Tóth, Ian Kitchen
    Abstract:

    Mu-opioid receptor knockout mice have been reported to show loss of some delta-opioid receptor function. We hypothesised that this is due to some delta-opioid receptors being uncoupled from G-proteins in the absence of the mu-opioid receptor. To address this possibility, we have used quantitative autoradiography to determine the binding of three delta-opioid receptor agonist ligands ([3H]deltorphin I, [3H] [R-Atc(3), Ile(5,6)]deltorphin II, [3H] 4-[(alpaR)-alpha-((2S,5R)-4-propyl-2,5-dimethyl-1-piperazinyl)-3-methoxybenzyl]-N,N-diethylbenzamide (SNC-121)) and the delta-opioid receptor antagonist, [3H]naltrindole in the presence and absence of a GTP analogue, Guanylylimidodiphosphate (GMPPNP) in the brains of mice lacking the mu-opioid receptor gene. Guanylylimidodiphosphate caused a decrease in the binding of the agonist ligands (to differing extents) and an increase in binding for the antagonist in wild-type controls. Overall, there were no major differences in the effects of Guanylylimidodiphosphate for the agonist ligands in mu-knockout mice although a few structures showed a smaller sensitivity to the effects of this GTP analogue most notably for [3H]naltrindole. These findings suggest that in the majority of brain regions, G-protein coupling is unaltered in mu-opioid receptor knockout mice.

  • G-protein coupling of δ-opioid receptors in brains of μ-opioid receptor knockout mice
    European Journal of Pharmacology, 2003
    Co-Authors: Sarah M. Oakley, Anna Borsodi, Brigitte L Kieffer, Géza Tóth, Ian Kitchen
    Abstract:

    Abstract μ-opioid receptor knockout mice have been reported to show loss of some δ-opioid receptor function. We hypothesised that this is due to some δ-opioid receptors being uncoupled from G-proteins in the absence of the μ-opioid receptor. To address this possibility, we have used quantitative autoradiography to determine the binding of three δ-opioid receptor agonist ligands ([ 3 H]deltorphin I, [ 3 H] [R-Atc 3 , Ile 5,6 ]deltorphin II, [ 3 H] 4-[(alpaR)-alpha-((2S,5R)-4-propyl-2,5-dimethyl-1-piperazinyl)-3-methoxybenzyl]- N , N -diethylbenzamide (SNC-121)) and the δ-opioid receptor antagonist, [ 3 H]naltrindole in the presence and absence of a GTP analogue, Guanylylimidodiphosphate (GMPPNP) in the brains of mice lacking the μ-opioid receptor gene. Guanylylimidodiphosphate caused a decrease in the binding of the agonist ligands (to differing extents) and an increase in binding for the antagonist in wild-type controls. Overall, there were no major differences in the effects of Guanylylimidodiphosphate for the agonist ligands in μ-knockout mice although a few structures showed a smaller sensitivity to the effects of this GTP analogue most notably for [ 3 H]naltrindole. These findings suggest that in the majority of brain regions, G-protein coupling is unaltered in μ-opioid receptor knockout mice.

Sarah M. Oakley - One of the best experts on this subject based on the ideXlab platform.

  • G-protein coupling of delta-opioid receptors in brains of mu-opioid receptor knockout mice.
    European journal of pharmacology, 2003
    Co-Authors: Sarah M. Oakley, Anna Borsodi, Brigitte L Kieffer, Géza Tóth, Ian Kitchen
    Abstract:

    Mu-opioid receptor knockout mice have been reported to show loss of some delta-opioid receptor function. We hypothesised that this is due to some delta-opioid receptors being uncoupled from G-proteins in the absence of the mu-opioid receptor. To address this possibility, we have used quantitative autoradiography to determine the binding of three delta-opioid receptor agonist ligands ([3H]deltorphin I, [3H] [R-Atc(3), Ile(5,6)]deltorphin II, [3H] 4-[(alpaR)-alpha-((2S,5R)-4-propyl-2,5-dimethyl-1-piperazinyl)-3-methoxybenzyl]-N,N-diethylbenzamide (SNC-121)) and the delta-opioid receptor antagonist, [3H]naltrindole in the presence and absence of a GTP analogue, Guanylylimidodiphosphate (GMPPNP) in the brains of mice lacking the mu-opioid receptor gene. Guanylylimidodiphosphate caused a decrease in the binding of the agonist ligands (to differing extents) and an increase in binding for the antagonist in wild-type controls. Overall, there were no major differences in the effects of Guanylylimidodiphosphate for the agonist ligands in mu-knockout mice although a few structures showed a smaller sensitivity to the effects of this GTP analogue most notably for [3H]naltrindole. These findings suggest that in the majority of brain regions, G-protein coupling is unaltered in mu-opioid receptor knockout mice.

  • G-protein coupling of δ-opioid receptors in brains of μ-opioid receptor knockout mice
    European Journal of Pharmacology, 2003
    Co-Authors: Sarah M. Oakley, Anna Borsodi, Brigitte L Kieffer, Géza Tóth, Ian Kitchen
    Abstract:

    Abstract μ-opioid receptor knockout mice have been reported to show loss of some δ-opioid receptor function. We hypothesised that this is due to some δ-opioid receptors being uncoupled from G-proteins in the absence of the μ-opioid receptor. To address this possibility, we have used quantitative autoradiography to determine the binding of three δ-opioid receptor agonist ligands ([ 3 H]deltorphin I, [ 3 H] [R-Atc 3 , Ile 5,6 ]deltorphin II, [ 3 H] 4-[(alpaR)-alpha-((2S,5R)-4-propyl-2,5-dimethyl-1-piperazinyl)-3-methoxybenzyl]- N , N -diethylbenzamide (SNC-121)) and the δ-opioid receptor antagonist, [ 3 H]naltrindole in the presence and absence of a GTP analogue, Guanylylimidodiphosphate (GMPPNP) in the brains of mice lacking the μ-opioid receptor gene. Guanylylimidodiphosphate caused a decrease in the binding of the agonist ligands (to differing extents) and an increase in binding for the antagonist in wild-type controls. Overall, there were no major differences in the effects of Guanylylimidodiphosphate for the agonist ligands in μ-knockout mice although a few structures showed a smaller sensitivity to the effects of this GTP analogue most notably for [ 3 H]naltrindole. These findings suggest that in the majority of brain regions, G-protein coupling is unaltered in μ-opioid receptor knockout mice.

Géza Tóth - One of the best experts on this subject based on the ideXlab platform.

  • G-protein coupling of delta-opioid receptors in brains of mu-opioid receptor knockout mice.
    European journal of pharmacology, 2003
    Co-Authors: Sarah M. Oakley, Anna Borsodi, Brigitte L Kieffer, Géza Tóth, Ian Kitchen
    Abstract:

    Mu-opioid receptor knockout mice have been reported to show loss of some delta-opioid receptor function. We hypothesised that this is due to some delta-opioid receptors being uncoupled from G-proteins in the absence of the mu-opioid receptor. To address this possibility, we have used quantitative autoradiography to determine the binding of three delta-opioid receptor agonist ligands ([3H]deltorphin I, [3H] [R-Atc(3), Ile(5,6)]deltorphin II, [3H] 4-[(alpaR)-alpha-((2S,5R)-4-propyl-2,5-dimethyl-1-piperazinyl)-3-methoxybenzyl]-N,N-diethylbenzamide (SNC-121)) and the delta-opioid receptor antagonist, [3H]naltrindole in the presence and absence of a GTP analogue, Guanylylimidodiphosphate (GMPPNP) in the brains of mice lacking the mu-opioid receptor gene. Guanylylimidodiphosphate caused a decrease in the binding of the agonist ligands (to differing extents) and an increase in binding for the antagonist in wild-type controls. Overall, there were no major differences in the effects of Guanylylimidodiphosphate for the agonist ligands in mu-knockout mice although a few structures showed a smaller sensitivity to the effects of this GTP analogue most notably for [3H]naltrindole. These findings suggest that in the majority of brain regions, G-protein coupling is unaltered in mu-opioid receptor knockout mice.

  • G-protein coupling of δ-opioid receptors in brains of μ-opioid receptor knockout mice
    European Journal of Pharmacology, 2003
    Co-Authors: Sarah M. Oakley, Anna Borsodi, Brigitte L Kieffer, Géza Tóth, Ian Kitchen
    Abstract:

    Abstract μ-opioid receptor knockout mice have been reported to show loss of some δ-opioid receptor function. We hypothesised that this is due to some δ-opioid receptors being uncoupled from G-proteins in the absence of the μ-opioid receptor. To address this possibility, we have used quantitative autoradiography to determine the binding of three δ-opioid receptor agonist ligands ([ 3 H]deltorphin I, [ 3 H] [R-Atc 3 , Ile 5,6 ]deltorphin II, [ 3 H] 4-[(alpaR)-alpha-((2S,5R)-4-propyl-2,5-dimethyl-1-piperazinyl)-3-methoxybenzyl]- N , N -diethylbenzamide (SNC-121)) and the δ-opioid receptor antagonist, [ 3 H]naltrindole in the presence and absence of a GTP analogue, Guanylylimidodiphosphate (GMPPNP) in the brains of mice lacking the μ-opioid receptor gene. Guanylylimidodiphosphate caused a decrease in the binding of the agonist ligands (to differing extents) and an increase in binding for the antagonist in wild-type controls. Overall, there were no major differences in the effects of Guanylylimidodiphosphate for the agonist ligands in μ-knockout mice although a few structures showed a smaller sensitivity to the effects of this GTP analogue most notably for [ 3 H]naltrindole. These findings suggest that in the majority of brain regions, G-protein coupling is unaltered in μ-opioid receptor knockout mice.

Anna Borsodi - One of the best experts on this subject based on the ideXlab platform.

  • G-protein coupling of delta-opioid receptors in brains of mu-opioid receptor knockout mice.
    European journal of pharmacology, 2003
    Co-Authors: Sarah M. Oakley, Anna Borsodi, Brigitte L Kieffer, Géza Tóth, Ian Kitchen
    Abstract:

    Mu-opioid receptor knockout mice have been reported to show loss of some delta-opioid receptor function. We hypothesised that this is due to some delta-opioid receptors being uncoupled from G-proteins in the absence of the mu-opioid receptor. To address this possibility, we have used quantitative autoradiography to determine the binding of three delta-opioid receptor agonist ligands ([3H]deltorphin I, [3H] [R-Atc(3), Ile(5,6)]deltorphin II, [3H] 4-[(alpaR)-alpha-((2S,5R)-4-propyl-2,5-dimethyl-1-piperazinyl)-3-methoxybenzyl]-N,N-diethylbenzamide (SNC-121)) and the delta-opioid receptor antagonist, [3H]naltrindole in the presence and absence of a GTP analogue, Guanylylimidodiphosphate (GMPPNP) in the brains of mice lacking the mu-opioid receptor gene. Guanylylimidodiphosphate caused a decrease in the binding of the agonist ligands (to differing extents) and an increase in binding for the antagonist in wild-type controls. Overall, there were no major differences in the effects of Guanylylimidodiphosphate for the agonist ligands in mu-knockout mice although a few structures showed a smaller sensitivity to the effects of this GTP analogue most notably for [3H]naltrindole. These findings suggest that in the majority of brain regions, G-protein coupling is unaltered in mu-opioid receptor knockout mice.

  • G-protein coupling of δ-opioid receptors in brains of μ-opioid receptor knockout mice
    European Journal of Pharmacology, 2003
    Co-Authors: Sarah M. Oakley, Anna Borsodi, Brigitte L Kieffer, Géza Tóth, Ian Kitchen
    Abstract:

    Abstract μ-opioid receptor knockout mice have been reported to show loss of some δ-opioid receptor function. We hypothesised that this is due to some δ-opioid receptors being uncoupled from G-proteins in the absence of the μ-opioid receptor. To address this possibility, we have used quantitative autoradiography to determine the binding of three δ-opioid receptor agonist ligands ([ 3 H]deltorphin I, [ 3 H] [R-Atc 3 , Ile 5,6 ]deltorphin II, [ 3 H] 4-[(alpaR)-alpha-((2S,5R)-4-propyl-2,5-dimethyl-1-piperazinyl)-3-methoxybenzyl]- N , N -diethylbenzamide (SNC-121)) and the δ-opioid receptor antagonist, [ 3 H]naltrindole in the presence and absence of a GTP analogue, Guanylylimidodiphosphate (GMPPNP) in the brains of mice lacking the μ-opioid receptor gene. Guanylylimidodiphosphate caused a decrease in the binding of the agonist ligands (to differing extents) and an increase in binding for the antagonist in wild-type controls. Overall, there were no major differences in the effects of Guanylylimidodiphosphate for the agonist ligands in μ-knockout mice although a few structures showed a smaller sensitivity to the effects of this GTP analogue most notably for [ 3 H]naltrindole. These findings suggest that in the majority of brain regions, G-protein coupling is unaltered in μ-opioid receptor knockout mice.

Brigitte L Kieffer - One of the best experts on this subject based on the ideXlab platform.

  • G-protein coupling of delta-opioid receptors in brains of mu-opioid receptor knockout mice.
    European journal of pharmacology, 2003
    Co-Authors: Sarah M. Oakley, Anna Borsodi, Brigitte L Kieffer, Géza Tóth, Ian Kitchen
    Abstract:

    Mu-opioid receptor knockout mice have been reported to show loss of some delta-opioid receptor function. We hypothesised that this is due to some delta-opioid receptors being uncoupled from G-proteins in the absence of the mu-opioid receptor. To address this possibility, we have used quantitative autoradiography to determine the binding of three delta-opioid receptor agonist ligands ([3H]deltorphin I, [3H] [R-Atc(3), Ile(5,6)]deltorphin II, [3H] 4-[(alpaR)-alpha-((2S,5R)-4-propyl-2,5-dimethyl-1-piperazinyl)-3-methoxybenzyl]-N,N-diethylbenzamide (SNC-121)) and the delta-opioid receptor antagonist, [3H]naltrindole in the presence and absence of a GTP analogue, Guanylylimidodiphosphate (GMPPNP) in the brains of mice lacking the mu-opioid receptor gene. Guanylylimidodiphosphate caused a decrease in the binding of the agonist ligands (to differing extents) and an increase in binding for the antagonist in wild-type controls. Overall, there were no major differences in the effects of Guanylylimidodiphosphate for the agonist ligands in mu-knockout mice although a few structures showed a smaller sensitivity to the effects of this GTP analogue most notably for [3H]naltrindole. These findings suggest that in the majority of brain regions, G-protein coupling is unaltered in mu-opioid receptor knockout mice.

  • G-protein coupling of δ-opioid receptors in brains of μ-opioid receptor knockout mice
    European Journal of Pharmacology, 2003
    Co-Authors: Sarah M. Oakley, Anna Borsodi, Brigitte L Kieffer, Géza Tóth, Ian Kitchen
    Abstract:

    Abstract μ-opioid receptor knockout mice have been reported to show loss of some δ-opioid receptor function. We hypothesised that this is due to some δ-opioid receptors being uncoupled from G-proteins in the absence of the μ-opioid receptor. To address this possibility, we have used quantitative autoradiography to determine the binding of three δ-opioid receptor agonist ligands ([ 3 H]deltorphin I, [ 3 H] [R-Atc 3 , Ile 5,6 ]deltorphin II, [ 3 H] 4-[(alpaR)-alpha-((2S,5R)-4-propyl-2,5-dimethyl-1-piperazinyl)-3-methoxybenzyl]- N , N -diethylbenzamide (SNC-121)) and the δ-opioid receptor antagonist, [ 3 H]naltrindole in the presence and absence of a GTP analogue, Guanylylimidodiphosphate (GMPPNP) in the brains of mice lacking the μ-opioid receptor gene. Guanylylimidodiphosphate caused a decrease in the binding of the agonist ligands (to differing extents) and an increase in binding for the antagonist in wild-type controls. Overall, there were no major differences in the effects of Guanylylimidodiphosphate for the agonist ligands in μ-knockout mice although a few structures showed a smaller sensitivity to the effects of this GTP analogue most notably for [ 3 H]naltrindole. These findings suggest that in the majority of brain regions, G-protein coupling is unaltered in μ-opioid receptor knockout mice.