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Francesc Piferrer - One of the best experts on this subject based on the ideXlab platform.
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Induction of Gynogenesis in the turbot (Scophthalmus maximus):: Effects of UV irradiation on sperm motility, the Hertwig effect and viability during the first 6 months of age
Aquaculture, 2004Co-Authors: Francesc Piferrer, Rosa Cal, Castora Gómez, Blanca Álvarez-blázquez, J. Castro, Paulino MartínezAbstract:Abstract Fish in which Gynogenesis has been induced have all their chromosomes inherited from the mother and, if females are the homogametic sex, they usually are all females. Because turbot females grow faster than males, the production of all-female populations is highly desirable. The sperm of turbot is of poor quality and its larvae are small and fragile. These circumstances represent a challenge for the induction of Gynogenesis in the turbot. As a first step towards this goal, effective conditions for the induction of Gynogenesis through UV irradiation of sperm followed by a cold shock were established. When diluted 1:10 with Ringer-200 saline solution and placed in a thin layer (∼0.3 mm), a dose-dependent effect of UV light on sperm motility was found. The dose at which both the amount of motile sperm and the duration of sperm motility was reduced to 50% of the original value (ID 50 ) was ∼28,000 erg mm −2 . A typical Hertwig effect was elicited with a dose of 30,000 erg mm −2 . The resulting embryos exhibited the typical “haploid syndrome” and died shortly after hatching. Application of a cold shock (−1 to 0°C for 25 min starting at 6.5 min after fertilization) to activated eggs with UV-irradiated (30,000 erg mm −2 ) and diluted (1:10) sperms restored diploidy and resulted in the production of gynogenetic diploids (2 n =44 chromosomes). These conditions were used in a pilot-scale experiment and found effective in inducing Gynogenesis in ∼47,000 eggs. The rate of Gynogenesis induction was 100% as verified by an analysis with microsatellite DNA markers. Survival of the gynogenetics was approximately 10% of diploids at 6 months of age, although growth was similar during this period. If this species turns out to have female homogamety, as is the case in most pleuronectiformes examined so far, the method presented here is the first necessary step for the production of all-female populations of this economically important species.
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Induction of Gynogenesis in the turbot (Scophthalmus maximus):
Aquaculture, 2004Co-Authors: Francesc Piferrer, Castora Gómez, Blanca Álvarez-blázquez, J. Castro, Rosa M. Cal, Paulino Martı́nezAbstract:17 figures, 8 pages, 1 table.-- Printed version published Sep 2004.Fish in which Gynogenesis has been induced have all their chromosomes inherited from the mother and, if females are the homogametic sex, they usually are all females. Because turbot females grow faster than males, the production of all-female populations is highly desirable. The sperm of turbot is of poor quality and its larvae are small and fragile. These circumstances represent a challenge for the induction of Gynogenesis in the turbot. As a first step towards this goal, effective conditions for the induction of Gynogenesis through UV irradiation of sperm followed by a cold shock were established. When diluted 1:10 with Ringer-200 saline solution and placed in a thin layer (~0.3 mm), a dose-dependent effect of UV light on sperm motility was found. The dose at which both the amount of motile sperm and the duration of sperm motility was reduced to 50% of the original value (ID-50) was ~28,000 erg mm−2. A typical Hertwig effect was elicited with a dose of 30,000 erg mm−2. The resulting embryos exhibited the typical “haploid syndrome” and died shortly after hatching. Application of a cold shock (−1 to 0°C for 25 min starting at 6.5 min after fertilization) to activated eggs with UV-irradiated (30,000 erg mm−2) and diluted (1:10) sperms restored diploidy and resulted in the production of gynogenetic diploids (2n=44 chromosomes). These conditions were used in a pilot-scale experiment and found effective in inducing Gynogenesis in ~47,000 eggs. The rate of Gynogenesis induction was 100% as verified by an analysis with microsatellite DNA markers. Survival of the gynogenetics was approximately 10% of diploids at 6 months of age, although growth was similar during this period. If this species turns out to have female homogamety, as is the case in most pleuronectiformes examined so far, the method presented here is the first necessary step for the production of all-female populations of this economically important species.Research funded by Spanish Government CICYT grant MAR95-1855 to P.M.Peer reviewe
Jian-fang Gui - One of the best experts on this subject based on the ideXlab platform.
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genetic identification of a newly synthetic allopolyploid strain with 206 chromosomes in polyploid gibel carp
Aquaculture Research, 2018Co-Authors: Zhongwei Wang, Li Zhou, Jian-fang GuiAbstract:A newly synthetic allopolyploid strain (SAS) was selected and established from gynogenetic offspring of gibel carp clone A(+) with 156 chromosomes induced by common carp sperm with 50 chromosomes. In this study, the allopolyploid strain was detected to contain 206 chromosomes, and the growth trait was evaluated to have 25.15% growth faster than that of clone A(+). Genetic marker analyses of transferrin (Tf) alleles, ITS1 sequences and mtDNA D-loop sequences indicated that the allopolyploid strain was synthetized from maternal gibel carp clone A(+) and paternal common carp, and the synthetic chromosome sets were further confirmed by genomic insitu hybridization (GISH) and chromosome localization of 45S rDNA. Significantly, the synthetic allopolyploid strain has tended to be stable by five successive generations of Gynogenesis, because it still keeps unisexual reproduction mode of Gynogenesis. Therefore, it will become a novel variety for gibel carp aquaculture in future.
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meiosis completion and various sperm responses lead to unisexual and sexual reproduction modes in one clone of polyploid carassius gibelio
Scientific Reports, 2015Co-Authors: Jun Zhang, Li Zhou, Min Sun, Zhen Liu, Xiaoli Liu, Wei Liu, Jian-fang GuiAbstract:Unisexual polyploid vertebrates are commonly known to reproduce by Gynogenesis, parthenogenesis, or hybridogenesis. One clone of polyploid Carassius gibelio has been revealed to possess multiple modes of unisexual Gynogenesis and sexual reproduction, but the cytological and developmental mechanisms have remained unknown. In this study, normal meiosis completion was firstly confirmed by spindle co-localization of β-tubulin and Spindlin. Moreover, three types of various nuclear events and development behaviors were revealed by DAPI staining and BrdU-incorporated immunofluorescence detection during the first mitosis in the fertilized eggs by three kinds of different sperms. They include normal sexual reproduction in response to sperm from the same clone male, typical unisexual Gynogenesis in response to sperm from the male of another species Cyprinus carpio, and an unusual hybrid-similar development mode in response to sperm from another different clone male. Based on these findings, we have discussed cytological and developmental mechanisms on multiple reproduction modes in the polyploid fish, and highlighted evolutionary significance of meiosis completion and evolutionary consequences of reproduction mode diversity in polyploid vertebrates.
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a novel nucleo cytoplasmic hybrid clone formed via androgenesis in polyploid gibel carp
BMC Research Notes, 2011Co-Authors: Zhongwei Wang, Li Zhou, Huaping Zhu, Da Wang, Fangfang Jiang, Wei Guo, Jian-fang GuiAbstract:Background Unisexual vertebrates have been demonstrated to reproduce by Gynogenesis, hybridogenesis, parthenogenesis, or kleptogenesis, however, it is uncertain how the reproduction mode contributes to the clonal diversity. Recently, polyploid gibel carp has been revealed to possess coexisting dual modes of unisexual Gynogenesis and sexual reproduction and to have numerous various clones. Using sexual reproduction mating between clone D female and clone A male and subsequent 7 generation multiplying of unisexual Gynogenesis, we have created a novel clone strain with more than several hundred millions of individuals. Here, we attempt to identify genetic background of the novel clone and to explore the significant implication for clonal diversity contribution.
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genetic basis and breeding application of clonal diversity and dual reproduction modes in polyploid carassius auratus gibelio
Science China-life Sciences, 2010Co-Authors: Jian-fang Gui, Li ZhouAbstract:A unisexual species is generally associated with polyploidy, and reproduced by a unisexual reproduction mode, such as Gynogenesis, hybridogenesis or parthenogenesis. Compared with other unisexual and polyploid species, gibel carp (Carassius auratus gibelio) has a higher ploidy level of hexaploid. It has undergone several successive rounds of genome polyploidy, and experienced an additional, more recent genome duplication event. More significantly, the dual reproduction modes, including Gynogenesis and sexual reproduction, have been demonstrated to coexist in the polyploid gibel carp. This article reviews the genetic basis concerning polyploidy origin, clonal diversity and dual reproduction modes, and outlines the progress in new variety breeding and gene identification involved in the reproduction and early development. The data suggests that gibel carp are under an evolutionary trajectory of diploidization. As a novel evolutionary developmental (Evo-Devo) biology model, this work highlights future perspectives about the functional divergence of duplicated genes and the sexual origin of vertebrate animals.
Paulino Martínez - One of the best experts on this subject based on the ideXlab platform.
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Induction of Gynogenesis in the turbot (Scophthalmus maximus):: Effects of UV irradiation on sperm motility, the Hertwig effect and viability during the first 6 months of age
Aquaculture, 2004Co-Authors: Francesc Piferrer, Rosa Cal, Castora Gómez, Blanca Álvarez-blázquez, J. Castro, Paulino MartínezAbstract:Abstract Fish in which Gynogenesis has been induced have all their chromosomes inherited from the mother and, if females are the homogametic sex, they usually are all females. Because turbot females grow faster than males, the production of all-female populations is highly desirable. The sperm of turbot is of poor quality and its larvae are small and fragile. These circumstances represent a challenge for the induction of Gynogenesis in the turbot. As a first step towards this goal, effective conditions for the induction of Gynogenesis through UV irradiation of sperm followed by a cold shock were established. When diluted 1:10 with Ringer-200 saline solution and placed in a thin layer (∼0.3 mm), a dose-dependent effect of UV light on sperm motility was found. The dose at which both the amount of motile sperm and the duration of sperm motility was reduced to 50% of the original value (ID 50 ) was ∼28,000 erg mm −2 . A typical Hertwig effect was elicited with a dose of 30,000 erg mm −2 . The resulting embryos exhibited the typical “haploid syndrome” and died shortly after hatching. Application of a cold shock (−1 to 0°C for 25 min starting at 6.5 min after fertilization) to activated eggs with UV-irradiated (30,000 erg mm −2 ) and diluted (1:10) sperms restored diploidy and resulted in the production of gynogenetic diploids (2 n =44 chromosomes). These conditions were used in a pilot-scale experiment and found effective in inducing Gynogenesis in ∼47,000 eggs. The rate of Gynogenesis induction was 100% as verified by an analysis with microsatellite DNA markers. Survival of the gynogenetics was approximately 10% of diploids at 6 months of age, although growth was similar during this period. If this species turns out to have female homogamety, as is the case in most pleuronectiformes examined so far, the method presented here is the first necessary step for the production of all-female populations of this economically important species.
Castora Gómez - One of the best experts on this subject based on the ideXlab platform.
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Induction of Gynogenesis in the turbot (Scophthalmus maximus):: Effects of UV irradiation on sperm motility, the Hertwig effect and viability during the first 6 months of age
Aquaculture, 2004Co-Authors: Francesc Piferrer, Rosa Cal, Castora Gómez, Blanca Álvarez-blázquez, J. Castro, Paulino MartínezAbstract:Abstract Fish in which Gynogenesis has been induced have all their chromosomes inherited from the mother and, if females are the homogametic sex, they usually are all females. Because turbot females grow faster than males, the production of all-female populations is highly desirable. The sperm of turbot is of poor quality and its larvae are small and fragile. These circumstances represent a challenge for the induction of Gynogenesis in the turbot. As a first step towards this goal, effective conditions for the induction of Gynogenesis through UV irradiation of sperm followed by a cold shock were established. When diluted 1:10 with Ringer-200 saline solution and placed in a thin layer (∼0.3 mm), a dose-dependent effect of UV light on sperm motility was found. The dose at which both the amount of motile sperm and the duration of sperm motility was reduced to 50% of the original value (ID 50 ) was ∼28,000 erg mm −2 . A typical Hertwig effect was elicited with a dose of 30,000 erg mm −2 . The resulting embryos exhibited the typical “haploid syndrome” and died shortly after hatching. Application of a cold shock (−1 to 0°C for 25 min starting at 6.5 min after fertilization) to activated eggs with UV-irradiated (30,000 erg mm −2 ) and diluted (1:10) sperms restored diploidy and resulted in the production of gynogenetic diploids (2 n =44 chromosomes). These conditions were used in a pilot-scale experiment and found effective in inducing Gynogenesis in ∼47,000 eggs. The rate of Gynogenesis induction was 100% as verified by an analysis with microsatellite DNA markers. Survival of the gynogenetics was approximately 10% of diploids at 6 months of age, although growth was similar during this period. If this species turns out to have female homogamety, as is the case in most pleuronectiformes examined so far, the method presented here is the first necessary step for the production of all-female populations of this economically important species.
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Induction of Gynogenesis in the turbot (Scophthalmus maximus):
Aquaculture, 2004Co-Authors: Francesc Piferrer, Castora Gómez, Blanca Álvarez-blázquez, J. Castro, Rosa M. Cal, Paulino Martı́nezAbstract:17 figures, 8 pages, 1 table.-- Printed version published Sep 2004.Fish in which Gynogenesis has been induced have all their chromosomes inherited from the mother and, if females are the homogametic sex, they usually are all females. Because turbot females grow faster than males, the production of all-female populations is highly desirable. The sperm of turbot is of poor quality and its larvae are small and fragile. These circumstances represent a challenge for the induction of Gynogenesis in the turbot. As a first step towards this goal, effective conditions for the induction of Gynogenesis through UV irradiation of sperm followed by a cold shock were established. When diluted 1:10 with Ringer-200 saline solution and placed in a thin layer (~0.3 mm), a dose-dependent effect of UV light on sperm motility was found. The dose at which both the amount of motile sperm and the duration of sperm motility was reduced to 50% of the original value (ID-50) was ~28,000 erg mm−2. A typical Hertwig effect was elicited with a dose of 30,000 erg mm−2. The resulting embryos exhibited the typical “haploid syndrome” and died shortly after hatching. Application of a cold shock (−1 to 0°C for 25 min starting at 6.5 min after fertilization) to activated eggs with UV-irradiated (30,000 erg mm−2) and diluted (1:10) sperms restored diploidy and resulted in the production of gynogenetic diploids (2n=44 chromosomes). These conditions were used in a pilot-scale experiment and found effective in inducing Gynogenesis in ~47,000 eggs. The rate of Gynogenesis induction was 100% as verified by an analysis with microsatellite DNA markers. Survival of the gynogenetics was approximately 10% of diploids at 6 months of age, although growth was similar during this period. If this species turns out to have female homogamety, as is the case in most pleuronectiformes examined so far, the method presented here is the first necessary step for the production of all-female populations of this economically important species.Research funded by Spanish Government CICYT grant MAR95-1855 to P.M.Peer reviewe
J. Castro - One of the best experts on this subject based on the ideXlab platform.
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Induction of Gynogenesis in the turbot (Scophthalmus maximus):: Effects of UV irradiation on sperm motility, the Hertwig effect and viability during the first 6 months of age
Aquaculture, 2004Co-Authors: Francesc Piferrer, Rosa Cal, Castora Gómez, Blanca Álvarez-blázquez, J. Castro, Paulino MartínezAbstract:Abstract Fish in which Gynogenesis has been induced have all their chromosomes inherited from the mother and, if females are the homogametic sex, they usually are all females. Because turbot females grow faster than males, the production of all-female populations is highly desirable. The sperm of turbot is of poor quality and its larvae are small and fragile. These circumstances represent a challenge for the induction of Gynogenesis in the turbot. As a first step towards this goal, effective conditions for the induction of Gynogenesis through UV irradiation of sperm followed by a cold shock were established. When diluted 1:10 with Ringer-200 saline solution and placed in a thin layer (∼0.3 mm), a dose-dependent effect of UV light on sperm motility was found. The dose at which both the amount of motile sperm and the duration of sperm motility was reduced to 50% of the original value (ID 50 ) was ∼28,000 erg mm −2 . A typical Hertwig effect was elicited with a dose of 30,000 erg mm −2 . The resulting embryos exhibited the typical “haploid syndrome” and died shortly after hatching. Application of a cold shock (−1 to 0°C for 25 min starting at 6.5 min after fertilization) to activated eggs with UV-irradiated (30,000 erg mm −2 ) and diluted (1:10) sperms restored diploidy and resulted in the production of gynogenetic diploids (2 n =44 chromosomes). These conditions were used in a pilot-scale experiment and found effective in inducing Gynogenesis in ∼47,000 eggs. The rate of Gynogenesis induction was 100% as verified by an analysis with microsatellite DNA markers. Survival of the gynogenetics was approximately 10% of diploids at 6 months of age, although growth was similar during this period. If this species turns out to have female homogamety, as is the case in most pleuronectiformes examined so far, the method presented here is the first necessary step for the production of all-female populations of this economically important species.
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Induction of Gynogenesis in the turbot (Scophthalmus maximus):
Aquaculture, 2004Co-Authors: Francesc Piferrer, Castora Gómez, Blanca Álvarez-blázquez, J. Castro, Rosa M. Cal, Paulino Martı́nezAbstract:17 figures, 8 pages, 1 table.-- Printed version published Sep 2004.Fish in which Gynogenesis has been induced have all their chromosomes inherited from the mother and, if females are the homogametic sex, they usually are all females. Because turbot females grow faster than males, the production of all-female populations is highly desirable. The sperm of turbot is of poor quality and its larvae are small and fragile. These circumstances represent a challenge for the induction of Gynogenesis in the turbot. As a first step towards this goal, effective conditions for the induction of Gynogenesis through UV irradiation of sperm followed by a cold shock were established. When diluted 1:10 with Ringer-200 saline solution and placed in a thin layer (~0.3 mm), a dose-dependent effect of UV light on sperm motility was found. The dose at which both the amount of motile sperm and the duration of sperm motility was reduced to 50% of the original value (ID-50) was ~28,000 erg mm−2. A typical Hertwig effect was elicited with a dose of 30,000 erg mm−2. The resulting embryos exhibited the typical “haploid syndrome” and died shortly after hatching. Application of a cold shock (−1 to 0°C for 25 min starting at 6.5 min after fertilization) to activated eggs with UV-irradiated (30,000 erg mm−2) and diluted (1:10) sperms restored diploidy and resulted in the production of gynogenetic diploids (2n=44 chromosomes). These conditions were used in a pilot-scale experiment and found effective in inducing Gynogenesis in ~47,000 eggs. The rate of Gynogenesis induction was 100% as verified by an analysis with microsatellite DNA markers. Survival of the gynogenetics was approximately 10% of diploids at 6 months of age, although growth was similar during this period. If this species turns out to have female homogamety, as is the case in most pleuronectiformes examined so far, the method presented here is the first necessary step for the production of all-female populations of this economically important species.Research funded by Spanish Government CICYT grant MAR95-1855 to P.M.Peer reviewe