The Experts below are selected from a list of 195 Experts worldwide ranked by ideXlab platform
Eric Delwart - One of the best experts on this subject based on the ideXlab platform.
-
A new Gyrovirus in human feces
Virus genes, 2015Co-Authors: Tung Gia Phan, Antonio Charlys Da Costa, Wen Zhang, Pierre Pothier, Katia Ambert-balay, Xutao Deng, Eric DelwartAbstract:A novel Gyrovirus genome found in the feces of an adult with diarrhea is described. The genome shows the three expected main ORFs encoding a structural protein (VP1), nonstructural protein (VP2), and Apoptin protein (VP3), which shared identities of 41, 42, and 38 % with those of the most closely related Gyrovirus proteins, respectively. Given the high divergence in its genome, this Gyrovirus may be considered the prototype for a new viral species (GyV9) in the Gyrovirus genus. Because the closest relatives of this Gyrovirus infect chicken, a possible dietary origin for the presence of this virus in human feces is discussed.
-
what is for dinner viral metagenomics of us store bought beef pork and chicken
Virology, 2014Co-Authors: Wen Zhang, Xutao Deng, Eric Delwart, Beatrix KapusinszkyAbstract:We describe here the metagenomics-derived viral sequences detected in beef, pork, and chicken purchased from stores in San Francisco. In beef we detected four previously reported viruses (two parvoviruses belonging to different genera, an anellovirus, and one circovirus-like virus) and one novel bovine polyomavirus species (BPyV2-SF) whose closest relatives infect primates. Detection of porcine hokovirus in beef indicated that this parvovirus can infect both ungulate species. In pork we detected four known parvoviruses from three genera, an anellovirus, and pig circovirus 2. Chicken meat contained numerous Gyrovirus sequences including those of chicken anemia virus and of a novel Gyrovirus species (GyV7-SF). Our results provide an initial characterization of some of the viruses commonly found in US store-bought meats which included a diverse group of parvoviruses and viral families with small circular DNA genomes. Whether any of these viruses can infect humans will require testing human sera for specific antibodies.
-
divergent Gyroviruses in the feces of tunisian children
Virology, 2013Co-Authors: Tung Gia Phan, Pierre Pothier, Xutao Deng, Eric Delwart, Khira Sdiriloulizi, Mahjoub Aouni, Katia AmbertbalayAbstract:The Gyrovirus genus consists of the immunosuppressive Chicken Anemia Virus (CAV) prototype and since 2011 three other viral species found in sera/tissues of chickens, human feces, and on human skin. Here the genomes of two other Gyrovirus species were characterized in diarrhea samples from Tunisian children whose main ORFs shared amino acid identity of 46–59% with those of the previously characterized Gyroviruses and were provisionally named GyV5 and GyV6. All currently known Gyroviruses grouped into two clades with distinct genomic features including replacement of the VP2 overlapping Apoptin gene with a distinct ORF of unknown function. Previous reports of Gyrovirus DNA in human blood and on human skins warrant studies of possible human tropisms for these newly characterized Gyroviruses.
-
a third Gyrovirus species in human faeces
Journal of General Virology, 2012Co-Authors: Christian M. Leutenegger, Tung Gia Phan, Eric Delwart, Miguel G Oryan, Hector D Cortes, Nora Mamani, Isidore Juste O Bonkoungou, Chunling WangAbstract:Until 2011 the genus Gyrovirus in the family Circoviridae consisted of a single virus (Chicken anemia virus or CAV) causing a common immunosuppressive disease in chickens when a second Gyrovirus (HGyV) was reported on the skin of 4 % of healthy humans. HGyV is very closely related to a recently described chicken Gyrovirus, AGV2, suggesting that they belong to the same viral species. During a viral metagenomic analysis of 100 human faeces from children with diarrhoea in Chile we identified multiple known human pathogens (adenoviruses, enteroviruses, astroviruses, sapoviruses, noroviruses, parechoviruses and rotaviruses) and a novel Gyrovirus species we named GyV3 sharing <63 % similarity with other Gyrovirus proteins with evidence of recombination with CAV in its UTR. Gyroviridae consensus PCR revealed a high prevalence of CAV DNA in diarrhoea and normal faeces from Chilean children and faeces of USA cats and dogs, which may reflect consumption of CAV-infected/vaccinated chickens. Whether GyV3 can infect humans and/or chickens requires further studies.
Tung Gia Phan - One of the best experts on this subject based on the ideXlab platform.
-
a Gyrovirus infecting a sea bird
Archives of Virology, 2015Co-Authors: Patricia A Pesavento, Tung Gia Phan, Wen Zhang, Xutao Deng, Anne M Gaynor, Rebecca S DuerrAbstract:We characterized the genome of a highly divergent Gyrovirus (GyV8) in the spleen and uropygial gland tissues of a diseased northern fulmar (Fulmarus glacialis), a pelagic bird beached in San Francisco, California. No other exogenous viral sequences could be identified using viral metagenomics. The small circular DNA genome shared no significant nucleotide sequence identity, and only 38-42 % amino acid sequence identity in VP1, with any of the previously identified Gyroviruses. GyV8 is the first member of the third major phylogenetic clade of this viral genus and the first Gyrovirus detected in an avian species other than chicken.
-
A new Gyrovirus in human feces
Virus genes, 2015Co-Authors: Tung Gia Phan, Antonio Charlys Da Costa, Wen Zhang, Pierre Pothier, Katia Ambert-balay, Xutao Deng, Eric DelwartAbstract:A novel Gyrovirus genome found in the feces of an adult with diarrhea is described. The genome shows the three expected main ORFs encoding a structural protein (VP1), nonstructural protein (VP2), and Apoptin protein (VP3), which shared identities of 41, 42, and 38 % with those of the most closely related Gyrovirus proteins, respectively. Given the high divergence in its genome, this Gyrovirus may be considered the prototype for a new viral species (GyV9) in the Gyrovirus genus. Because the closest relatives of this Gyrovirus infect chicken, a possible dietary origin for the presence of this virus in human feces is discussed.
-
sequence and phylogenetic analysis identifies a putative novel Gyrovirus 3 genotype in ferret feces
Virus Genes, 2015Co-Authors: Enikő Feher, Tung Gia Phan, Peter Pazar, Gyorgy Lengyel, Krisztian BanyaiAbstract:The genomic sequence of a novel Gyrovirus (GyV) 3 strain was detected from the fecal sample of a pet ferret. The length (2,359 nt) and the basic genomic structure of this strain was very similar to that of the single known GyV3 reference strain, whereas the genome sequence identity between the two strains was only 76 %. Similarly, moderate sequence homology was found within the predicted protein coding regions, VP1 (nt, 72 %; aa, 76 %), VP2 (nt, 84 %; aa, 85 %), and VP3 (nt, 85 %; aa, 73 %). Sequence identities were lower when comparing our strain with other GyV species (48–65 % genome-wide nt identity). Phylogenetic analysis of the coding regions clustered the ferret origin GyV3 strain within Clade A. Although the available whole genomic sequence of novel GyVs permits limited conclusions to be drawn regarding the classification of the Hungarian GyV3 strain, our data indicate that this novel strain may be considered as a new genotype within GyV3. Further investigations are needed to reveal the genetic diversity and biological properties of newly described members of the Gyrovirus genus.
-
divergent Gyroviruses in the feces of tunisian children
Virology, 2013Co-Authors: Tung Gia Phan, Pierre Pothier, Xutao Deng, Eric Delwart, Khira Sdiriloulizi, Mahjoub Aouni, Katia AmbertbalayAbstract:The Gyrovirus genus consists of the immunosuppressive Chicken Anemia Virus (CAV) prototype and since 2011 three other viral species found in sera/tissues of chickens, human feces, and on human skin. Here the genomes of two other Gyrovirus species were characterized in diarrhea samples from Tunisian children whose main ORFs shared amino acid identity of 46–59% with those of the previously characterized Gyroviruses and were provisionally named GyV5 and GyV6. All currently known Gyroviruses grouped into two clades with distinct genomic features including replacement of the VP2 overlapping Apoptin gene with a distinct ORF of unknown function. Previous reports of Gyrovirus DNA in human blood and on human skins warrant studies of possible human tropisms for these newly characterized Gyroviruses.
-
a third Gyrovirus species in human faeces
Journal of General Virology, 2012Co-Authors: Christian M. Leutenegger, Tung Gia Phan, Eric Delwart, Miguel G Oryan, Hector D Cortes, Nora Mamani, Isidore Juste O Bonkoungou, Chunling WangAbstract:Until 2011 the genus Gyrovirus in the family Circoviridae consisted of a single virus (Chicken anemia virus or CAV) causing a common immunosuppressive disease in chickens when a second Gyrovirus (HGyV) was reported on the skin of 4 % of healthy humans. HGyV is very closely related to a recently described chicken Gyrovirus, AGV2, suggesting that they belong to the same viral species. During a viral metagenomic analysis of 100 human faeces from children with diarrhoea in Chile we identified multiple known human pathogens (adenoviruses, enteroviruses, astroviruses, sapoviruses, noroviruses, parechoviruses and rotaviruses) and a novel Gyrovirus species we named GyV3 sharing <63 % similarity with other Gyrovirus proteins with evidence of recombination with CAV in its UTR. Gyroviridae consensus PCR revealed a high prevalence of CAV DNA in diarrhoea and normal faeces from Chilean children and faeces of USA cats and dogs, which may reflect consumption of CAV-infected/vaccinated chickens. Whether GyV3 can infect humans and/or chickens requires further studies.
Youcheng Hseu - One of the best experts on this subject based on the ideXlab platform.
-
high yield expression in a recombinant e coli of a codon optimized chicken anemia virus capsid protein vp1 useful for vaccine development
Microbial Cell Factories, 2011Co-Authors: Youcheng Hseu, Wente Chang, Hsijien Chen, Chihung Huang, Minying Wang, Yiyang LienAbstract:Background Chicken anemia virus (CAV), the causative agent chicken anemia, is the only member of the genus Gyrovirus of the Circoviridae family. CAV is an immune suppressive virus and causes anemia, lymph organ atrophy and immunodeficiency. The production and biochemical characterization of VP1 protein and its use in a subunit vaccine or as part of a diagnostic kit would be useful to CAV infection prevention.
-
High yield expression in a recombinant E. coli of a codon optimized chicken anemia virus capsid protein VP1 useful for vaccine development
Microbial Cell Factories, 2011Co-Authors: Mengshiou Lee, Youcheng Hseu, Wente Chang, Hsijien Chen, Chihung Huang, Minying Wang, Mengshiunn Lee, Guan-hua Lai, Jung-yie Kao, Bang-jau YouAbstract:Background Chicken anemia virus (CAV), the causative agent chicken anemia, is the only member of the genus Gyrovirus of the Circoviridae family. CAV is an immune suppressive virus and causes anemia, lymph organ atrophy and immunodeficiency. The production and biochemical characterization of VP1 protein and its use in a subunit vaccine or as part of a diagnostic kit would be useful to CAV infection prevention. Results Significantly increased expression of the recombinant full-length VP1 capsid protein from chicken anemia virus was demonstrated using an E. coli expression system. The VP1 gene was cloned into various different expression vectors and then these were expressed in a number of different E. coli strains. The expression of CAV VP1 in E. coli was significantly increased when VP1 was fused with GST protein rather than a His-tag. By optimizing the various rare amino acid codons within the N-terminus of the VP1 protein, the expression level of the VP1 protein in E. coli BL21(DE3)-pLysS was further increased significantly. The highest protein expression level obtained was 17.5 g/L per liter of bacterial culture after induction with 0.1 mM IPTG for 2 h. After purification by GST affinity chromatography, the purified full-length VP1 protein produced in this way was demonstrated to have good antigenicity and was able to be recognized by CAV-positive chicken serum in an ELISA assay. Conclusions Purified recombinant VP1 protein with the gene's codons optimized in the N-terminal region has potential as chimeric protein that, when expressed in E. coli , may be useful in the future for the development of subunit vaccines and diagnostic tests.
Paulo Michel Roehe - One of the best experts on this subject based on the ideXlab platform.
-
viral metagenomics in brazilian pekin ducks identifies two Gyrovirus including a new species and the potentially pathogenic duck circovirus
Virology, 2020Co-Authors: Samuel Paulo Cibulski, Helton Fernandes Dos Santos, Thais Fumaco Teixeira, Ana Paula Muterle Varela, Francisco Esmaile De Sales Lima, Caroline Tochetto, Diane Alves De Lima, Fabiana Quoos Mayer, Matheus Nunes Weber, Paulo Michel RoeheAbstract:Viral metagenomics coupled to high-throughput sequencing has provided a powerful tool for large-scale detection of known and unknown viruses associated to distinct hosts and environments. Using this approach, known and novel viruses have been characterized from sylvatic and commercial avian hosts, increasing our understanding of the viral diversity in these species. In the present work we applied an exploratory viral metagenomics on organs (spleen, liver and bursa of Fabricious) of Pekin ducks from Southern Brazil. The virome contained sequences related to a known duck pathogen (duck circovirus) and a number of other circular ssDNA viruses. Additionally, we detected avian Gyrovirus 9 (to date detected only in human feces) and one new avian Gyrovirus species, to which is proposed the name avian Gyrovirus 13 (GyV13). This study is expected to contribute to the knowledge of the viral diversity in Pekin ducks.
-
Chicken anemia virus and avian Gyrovirus 2 as contaminants in poultry vaccines
Biologicals : journal of the International Association of Biological Standardization, 2014Co-Authors: Ana Paula Muterle Varela, Samuel Paulo Cibulski, Helton Fernandes Dos Santos, Ana Cláudia Franco, Camila Mengue Scheffer, Candice Schmidt, Francisco Esmaile De Sales Lima, Alessandra D'avila Da Silva, Paulo Augusto Esteves, Paulo Michel RoeheAbstract:This study focuses on the detection of chicken anemia virus (CAV) and avian Gyrovirus 2 (AGV2) genomes in commercially available poultry vaccines. A duplex quantitative real-time PCR (dqPCR), capable of identifying genomes of both viruses in a single assay, was employed to determine the viral loads of these agents in commercially available vaccines. Thirty five vaccines from eight manufacturers (32 prepared with live and 3 with inactivated microorganisms) were examined. Genomes of CAV were detected as contaminants in 6/32 live vaccines and in 1/3 inactivated vaccines. The CAV genome loads ranged from 6.4 to 173.4 per 50 ng of vaccine DNA (equivalent to 0.07 to 0.69 genome copies per dose of vaccine). Likewise, AGV2 genomes were detected in 9/32 live vaccines, with viral loads ranging from 93 to 156,187 per 50 ng of vaccine DNA (equivalent to 0.28e9176 genome copies per dose of vaccine). These findings provide evidence for the possibility of contamination of poultry vaccines with CAV and AGV2 and they also emphasize the need of searching for these agents in vaccines in order to ensure the absence of such potential contaminants.
-
discovery of a genome of a distant relative of chicken anemia virus reveals a new member of the genus Gyrovirus
Archives of Virology, 2011Co-Authors: Franciscus Antonius Maria Rijsewijk, Samuel Paulo Cibulski, Helton Fernandes Dos Santos, Thais Fumaco Teixeira, Ana Paula Muterle Varela, Ana Cláudia Franco, Diogenes Dezen, Paulo Michel RoeheAbstract:A 2.4-kb phi29 polymerase amplification product from serum of a diseased chicken was cloned and sequenced. The 2383-nucleotide sequence showed about 40% identity to a representative genome of chicken anemia virus (CAV), the only member of the genus Gyrovirus, family Circoviridae. The new genome had an organization similar to that of CAV: a putative 5′ untranscribed region of about 400 nt followed by three partially overlapping open reading frames encoding VP1, VP2 and VP3 homologs. The amino acid identities between these homologs and those of CAV were 38.8%, 40.3%, and 32.2%, respectively. Based on these limited similarities, it is proposed that the newly identified virus is a member of a new species in the genus Gyrovirus. For this new species, the name Avian Gyrovirus 2 (AGV2) is proposed.
Bang-jau You - One of the best experts on this subject based on the ideXlab platform.
-
High yield expression in a recombinant E. coli of a codon optimized chicken anemia virus capsid protein VP1 useful for vaccine development
Microbial Cell Factories, 2011Co-Authors: Mengshiou Lee, Youcheng Hseu, Wente Chang, Hsijien Chen, Chihung Huang, Minying Wang, Mengshiunn Lee, Guan-hua Lai, Jung-yie Kao, Bang-jau YouAbstract:Background Chicken anemia virus (CAV), the causative agent chicken anemia, is the only member of the genus Gyrovirus of the Circoviridae family. CAV is an immune suppressive virus and causes anemia, lymph organ atrophy and immunodeficiency. The production and biochemical characterization of VP1 protein and its use in a subunit vaccine or as part of a diagnostic kit would be useful to CAV infection prevention. Results Significantly increased expression of the recombinant full-length VP1 capsid protein from chicken anemia virus was demonstrated using an E. coli expression system. The VP1 gene was cloned into various different expression vectors and then these were expressed in a number of different E. coli strains. The expression of CAV VP1 in E. coli was significantly increased when VP1 was fused with GST protein rather than a His-tag. By optimizing the various rare amino acid codons within the N-terminus of the VP1 protein, the expression level of the VP1 protein in E. coli BL21(DE3)-pLysS was further increased significantly. The highest protein expression level obtained was 17.5 g/L per liter of bacterial culture after induction with 0.1 mM IPTG for 2 h. After purification by GST affinity chromatography, the purified full-length VP1 protein produced in this way was demonstrated to have good antigenicity and was able to be recognized by CAV-positive chicken serum in an ELISA assay. Conclusions Purified recombinant VP1 protein with the gene's codons optimized in the N-terminal region has potential as chimeric protein that, when expressed in E. coli , may be useful in the future for the development of subunit vaccines and diagnostic tests.