The Experts below are selected from a list of 6090 Experts worldwide ranked by ideXlab platform
Anna Katarzyna Wronska - One of the best experts on this subject based on the ideXlab platform.
-
Harman and norHarman metabolites of the entomopathogenic fungus conidiobolus coronatus entomophthorales affect the serotonin levels and phagocytic activity of hemocytes insect immunocompetent cells in galleria mellonella lepidoptera
Cell & Bioscience, 2019Co-Authors: Anna Katarzyna Wronska, Mieczyslawa I BoguśAbstract:Background Although the β-carboline alkaloids Harman and norHarman are considered as plant metabolites, they can also be secreted by fungi such as the entomopathogen Conidiobolus coronatus. NorHarman and Harman are also known to be reversible competitive monamine oxidase inhibitors, which increase serotonin concentrations in tissues. In addition, these alkaloids are able to bind to serotonin receptors, an important immune regulatory molecule in both vertebrates and invertebrates. In insects, serotonin modulates hemocyte phagocytosis, nodule formation and the populations of hemocyte classes. The present study examines whether Harman and norHarman may influence the phagocytic activity of insect hemocytes by regulating serotonin levels.
-
Harman and norHarman metabolites of the entomopathogenic fungus conidiobolus coronatus entomophthorales affect the serotonin levels and phagocytic activity of hemocytes insect immunocompetent cells in galleria mellonella lepidoptera
Cell & Bioscience, 2019Co-Authors: Anna Katarzyna WronskaAbstract:Although the β-carboline alkaloids Harman and norHarman are considered as plant metabolites, they can also be secreted by fungi such as the entomopathogen Conidiobolus coronatus. NorHarman and Harman are also known to be reversible competitive monamine oxidase inhibitors, which increase serotonin concentrations in tissues. In addition, these alkaloids are able to bind to serotonin receptors, an important immune regulatory molecule in both vertebrates and invertebrates. In insects, serotonin modulates hemocyte phagocytosis, nodule formation and the populations of hemocyte classes. The present study examines whether Harman and norHarman may influence the phagocytic activity of insect hemocytes by regulating serotonin levels. Significantly greater serotonin levels and hemocyte phagocytic activity were observed after 24 h of exposure to food contaminated with Harman and norHarman. Similar responses were noticed 1 h after topical application or addition to in vitro hemocyte cultures. Observations and measurements performed 24 h later revealed decreased responses, suggesting decomposition and/or exertion of alkaloids and/or serotonin. Harman and norHarman influenced the activity of Galleria mellonella plasmatocytes and the granulocyte cytoskeleton. Disturbances in hemocyte network formation, abnormal cell shape, naked nuclei, cell aggregates, fragments of disintegrated cells, interrupted cell membrane continuity and actin condensation in cells were observed. Our findings may have a considerable impact on research concerning insect physiology, parasitology, immunology and biocontrol of pests. They confirm for the first time that Harman and norHarman (metabolites of the entomopathogenic fungus C. coronatus) elevate serotonin levels in G. mellonella hemocytes, thus potentially stimulating their phagocytic activity. Our studies shed light on the mechanisms underlying the interaction between innate insect immune responses and entomopathogen metabolites.
-
Harman and norHarman metabolites of entomopathogenic fungus conidiobolus coronatus entomopthorales disorganize development of galleria mellonella lepidoptera and affect serotonin regulating enzymes
PLOS ONE, 2018Co-Authors: Anna Katarzyna Wronska, Agata Kaczmarek, Michalina KazekAbstract:: Naturally occurring entomopathogenic fungi such as Conidiobolus coronatus are important regulatory factors of insect populations. GC-MS analysis of fungal cell-free filtrates showed that C. coronatus synthesizes two β- carboline alkaloids: Harman and norHarman. Significantly higher levels of both alkaloids are produced by C. coronatus in minimal postincubation medium than in rich medium. The beta-carboline alkaloids may have an effect on the nervous system of insects and their behavior. Harman and norHarman were applied to Galleria mellonella larvae (a parasite of honeybees) either topically or mixed with food. Larvae received alkaloids in three concentrations: 750, 1000 or 1250 ppm. The effect on the survival and further development of larvae was examined. Both Harman and norHarman delayed pupation and adult eclosion, and inhibit total monoamine oxidase activity. In addition, they increased the serotonin concentration and decreased the monoamine oxidase A level in the heads of the moths. It is likely that the alkaloids were metabolized by the insects, as their effect wore off 24 hours after topical application. This is the first study to show that C. coronatus produces alkaloids. Its aim was to identify the actions of β-carboline alkaloids on insect development and serotonin-regulating enzymes. Knowledge of the potential role of Harman and norHarman in the process of fungal infection might lead to the development of more effective and environmentally-friendly means of controlling insect pests.
Mieczyslawa I Boguś - One of the best experts on this subject based on the ideXlab platform.
-
Harman and norHarman metabolites of the entomopathogenic fungus conidiobolus coronatus entomophthorales affect the serotonin levels and phagocytic activity of hemocytes insect immunocompetent cells in galleria mellonella lepidoptera
Cell & Bioscience, 2019Co-Authors: Anna Katarzyna Wronska, Mieczyslawa I BoguśAbstract:Background Although the β-carboline alkaloids Harman and norHarman are considered as plant metabolites, they can also be secreted by fungi such as the entomopathogen Conidiobolus coronatus. NorHarman and Harman are also known to be reversible competitive monamine oxidase inhibitors, which increase serotonin concentrations in tissues. In addition, these alkaloids are able to bind to serotonin receptors, an important immune regulatory molecule in both vertebrates and invertebrates. In insects, serotonin modulates hemocyte phagocytosis, nodule formation and the populations of hemocyte classes. The present study examines whether Harman and norHarman may influence the phagocytic activity of insect hemocytes by regulating serotonin levels.
Aruna Asista, Npm : 168090033 - One of the best experts on this subject based on the ideXlab platform.
-
KAJIAN PRAGMATIK TERHADAP KESANTUNAN BERBAHASA BERBASIS KEARIFAN LOKAL DALAM HARIAN UMUM PALEMBANG POS SEBAGAI UPAYA PENYUSUNAN BAHAN AJAR MENGANALISIS ISI STRUKTUR TEKS NEGOSIASI BAHASA INDONESIA KELAS X DI SMK
2019Co-Authors: Aruna Asista, Npm : 168090033Abstract:Asista, Aruna. 2019. Kajian Pragmatik terhadap Kesantunan Berbahasa Berbasis Kearifan Lokal dalam Harian Umum Palembang Pos sebagai Upaya Penyusunan Bahan Ajar Menganalisis Isi Struktur Teks Negosiasi Bahasa Indonesia Kelas X Di SMK. Tesis pada Program Studi Magister Pendidikan Bahasa Indonesia, Program Pascasarjana Universitas Pasundan Bandung. Pembimbing (1) Dr. Hj. Titin Nurhayatin, M.Pd. (2) Dr. H. Kunkun K Harnadi, M,Pd. Kata Kunci: rubrik, pragmatik, kesantunan berbahasa, kearifan lokal, modul Rubrik “Mang Juhai” harian umum Palembang Pos tidak semuanya dapat diterima oleh masyarakat. Hal ini tidak terlepas dengan budaya atau nilai kesantunan yang digunakan masyarakat. Penelitian kesantunan dipandang sebagai pengaitan bahasa dengan jenis tindak tutur yang sesuai dengan konteks sehingga komunikatif. Tujuan penelitian ini untuk mendeskripsikan kajian pragmatik terhadap kesantunan berbahasa berbasis kearifan lokal dalam harian umum palembang pos sebagai upaya penyusunan bahan ajar menganalisis isi struktur teks negosiasi bahasa Indonesia kelas X di SMK. Penelitian menggunakan metode deskriptif analisis. Berikut temuan dalam penulisan tesis ini, yaitu: (1) kajian pragmatik yaitu jenis tindak tutur yang terdapat dalam rubrik “Mang Juhai” harian umum Palembang Pos, yang meliputi lokusi, ilokusi, dan perlokusi, (2) prinsip kesantunan yang digunakan dalam rubrik “Mang Juhai” harian umum Palembang Pos, yang meliputi kearifan, kedermawanan, pujian, kerendahan hati, kesepakatan, dan kesimpatian, (3) nilai kearifan lokal Melayu masyarakat Palembang dalam rubrik “Mang Juhai harian umum Palembang Pos, yang meliputi Nilai Manusia terhadap Pribadi (NMP), Nilai Manusia terhadap Tuhan (NMT), Nilai Manusia terhadap Manusia (NMM), Nilai Manusia terhadap Alam (NMA), Nilai Manusia terhadap Waktu (NMW), dan Nilai Manusia terhadap Lahir-Batin (NMLB) dan (4) hasil kajian pragmatik, prinsip kesantunan berbahasa, dan nilai kearifan lokal Melayu pada rubrik “Mang Juhai” harian umum Palembang Pos selanjutnya dimanfaatkan sebagai modul bahan ajar kebahasaan sesuai Kurikulum 2013 Edisi Revisi 2016. Setelah melaui tahap validasi, modul dinyatakan layak untuk digunakan dalam kegiatan pengajaran kebahasaan di SMK
Asista Aruna - One of the best experts on this subject based on the ideXlab platform.
-
KAJIAN PRAGMATIK TERHADAP KESANTUNAN BERBAHASA BERBASIS KEARIFAN LOKAL DALAM HARIAN UMUM PALEMBANG POS SEBAGAI UPAYA PENYUSUNAN BAHAN AJAR MENGANALISIS ISI STRUKTUR TEKS NEGOSIASI BAHASA INDONESIA KELAS X DI SMK
Program Studi Magister Pendidikan Bahasa Indonesia Program Pascasarjana Universitas Pasundan Bandung, 2020Co-Authors: Asista ArunaAbstract:Rubrik “Mang Juhai” harian umum Palembang Pos tidak semuanya dapat diterima oleh masyarakat. Hal ini tidak terlepas dengan budaya atau nilai kesantunan yang digunakan masyarakat. Penelitian kesantunan dipandang sebagai pengaitan bahasa dengan jenis tindak tutur yang sesuai dengan konteks sehingga komunikatif. Tujuan penelitian ini untuk mendeskripsikan kajian pragmatik terhadap kesantunan berbahasa berbasis kearifan lokal dalam harian umum palembang pos sebagai upaya penyusunan bahan ajar menganalisis isi struktur teks negosiasi bahasa Indonesia kelas X di SMK. Penelitian menggunakan metode deskriptif analisis. Berikut temuan dalam penulisan tesis ini, yaitu: (1) kajian pragmatik yaitu jenis tindak tutur yang terdapat dalam rubrik “Mang Juhai” harian umum Palembang Pos, yang meliputi lokusi, ilokusi, dan perlokusi, (2) prinsip kesantunan yang digunakan dalam rubrik “Mang Juhai” harian umum Palembang Pos, yang meliputi kearifan, kedermawanan, pujian, kerendahan hati, kesepakatan, dan kesimpatian, (3) nilai kearifan lokal Melayu masyarakat Palembang dalam rubrik “Mang Juhai harian umum Palembang Pos, yang meliputi Nilai Manusia terhadap Pribadi (NMP), Nilai Manusia terhadap Tuhan (NMT), Nilai Manusia terhadap Manusia (NMM), Nilai Manusia terhadap Alam (NMA), Nilai Manusia terhadap Waktu (NMW), dan Nilai Manusia terhadap Lahir-Batin (NMLB) dan (4) hasil kajian pragmatik, prinsip kesantunan berbahasa, dan nilai kearifan lokal Melayu pada rubrik “Mang Juhai” harian umum Palembang Pos selanjutnya dimanfaatkan sebagai modul bahan ajar kebahasaan sesuai Kurikulum 2013 Edisi Revisi 2016. Setelah melaui tahap validasi, modul dinyatakan layak untuk digunakan dalam kegiatan pengajaran kebahasaan di SMK
Michalina Kazek - One of the best experts on this subject based on the ideXlab platform.
-
Harman and norHarman metabolites of entomopathogenic fungus conidiobolus coronatus entomopthorales disorganize development of galleria mellonella lepidoptera and affect serotonin regulating enzymes
PLOS ONE, 2018Co-Authors: Anna Katarzyna Wronska, Agata Kaczmarek, Michalina KazekAbstract:: Naturally occurring entomopathogenic fungi such as Conidiobolus coronatus are important regulatory factors of insect populations. GC-MS analysis of fungal cell-free filtrates showed that C. coronatus synthesizes two β- carboline alkaloids: Harman and norHarman. Significantly higher levels of both alkaloids are produced by C. coronatus in minimal postincubation medium than in rich medium. The beta-carboline alkaloids may have an effect on the nervous system of insects and their behavior. Harman and norHarman were applied to Galleria mellonella larvae (a parasite of honeybees) either topically or mixed with food. Larvae received alkaloids in three concentrations: 750, 1000 or 1250 ppm. The effect on the survival and further development of larvae was examined. Both Harman and norHarman delayed pupation and adult eclosion, and inhibit total monoamine oxidase activity. In addition, they increased the serotonin concentration and decreased the monoamine oxidase A level in the heads of the moths. It is likely that the alkaloids were metabolized by the insects, as their effect wore off 24 hours after topical application. This is the first study to show that C. coronatus produces alkaloids. Its aim was to identify the actions of β-carboline alkaloids on insect development and serotonin-regulating enzymes. Knowledge of the potential role of Harman and norHarman in the process of fungal infection might lead to the development of more effective and environmentally-friendly means of controlling insect pests.
