The Experts below are selected from a list of 312 Experts worldwide ranked by ideXlab platform
Eric E Allen - One of the best experts on this subject based on the ideXlab platform.
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microbial ecology of atlantic salmon salmo salar Hatcheries impacts of the built environment on fish mucosal microbiota
Applied and Environmental Microbiology, 2020Co-Authors: Jj Minich, Jp Bowman, Greg D Poore, Khattapan Jantawongsri, Colin Johnston, Kate Bowie, Rob Knight, B F Nowak, Eric E AllenAbstract:Successful rearing of fish in Hatcheries is critical for conservation, recreational fishing, commercial fishing through wild stock enhancements, and aquaculture production. Flow through (FT) Hatcheries require more water than Recirculating-Aquaculture-Systems (RAS) which enable up to 99% of water to be recycled thus significantly reducing environmental impacts. Here, we evaluated the biological and physical microbiome interactions of three Atlantic salmon Hatcheries (RAS n=2, FT n=1). Gill, skin, and digesta from six juvenile fish along with tank biofilms and water were sampled from tanks in each of the Hatcheries (60 fish across 10 tanks) to assess the built environment and mucosal microbiota using 16S rRNA gene sequencing. The water and tank biofilm had more microbial richness than fish mucus while skin and digesta from RAS fish had 2x the richness of FT fish. Body sites each had unique microbiomes (P IMPORTANCE Atlantic salmon, Salmo salar, is the most farmed marine fish worldwide with an annual production of 2,248 million metric tonnes in 2016. Salmon Hatcheries are increasingly changing from flow through towards RAS design to accommodate more control over production along with improved environmental sustainability due to lower impacts on water consumption. To date, microbiome studies on Hatcheries have focused either on the fish mucosal microbiota or the built environment microbiota, but have not combined the two to understand interactions. Our study evaluates how water and tank biofilm microbiota influences fish microbiota across three mucosal environments (gill, skin, and digesta). Results from this study highlight how the built environment is a unique source of microbes to colonize fish mucus and furthermore how this can influence fish health. Further studies can use this knowledge to engineer built environments to modulate fish microbiota for beneficial phenotypes.
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microbial ecology of atlantic salmon salmo salar Hatcheries impacts of the built environment on fish mucosal microbiota
bioRxiv, 2019Co-Authors: Jj Minich, Jp Bowman, Khattapan Jantawongsri, Colin Johnston, Kate Bowie, Rob Knight, B F Nowak, Eric E AllenAbstract:Successful rearing of fish in Hatcheries is critical for conservation, recreational fishing, and commercial fishing through wild stock enhancements, and aquaculture production. Flow through (FT) Hatcheries require more water than Recirculating-Aquaculture-Systems (RAS) which enable up to 99% of water to be recycled thus significantly reducing environmental impacts. Here, we evaluated the biological and physical microbiome interactions of the built environment of a hatchery from three Atl salmon Hatcheries (RAS n=2, FT n=1). Six juvenile fish were sampled from tanks in each of the Hatcheries for a total of 60 fish across 10 tanks. Water and tank side biofilm samples were collected from each of the tanks along with three salmon body sites (gill, skin, and digesta) to assess mucosal microbiota using 16S rRNA sequencing. The water and tank biofilm had more microbial richness than fish mucus while skin and digesta from RAS fish had 2x the richness of FT fish. Body sites each had unique microbial communities (P<0.001) and were influenced by the various hatchery systems (P<0.001) with RAS systems more similar. Water and especially tank biofilm richness was positively correlated with skin and digesta richness. Strikingly, the gill, skin and digesta communities were more similar to the origin tank biofilm vs. all other experimental tanks suggesting that the tank biofilm has a direct influence on fish-associated microbial communities. The results from this study provide evidence for a link between the tank microbiome and the fish microbiome with the skin microbiome as an important intermediate.nnIMPORTANCEAtlantic salmon, Salmo salar, is the most farmed marine fish worldwide with an annual production of 2,248 million metric tonnes in 2016. Salmon Hatcheries are increasingly changing from flow through towards RAS design to accommodate more control over production along with improved environmental sustainability due to lower impacts on water consumption. To date, microbiome studies on Hatcheries have focused either on the fish mucosal microbiota or the built environment microbiota, but have not combined the two to understand interactions. Our study evaluates how water and tank biofilm microbiota influences fish microbiota across three mucosal environments (gill, skin, and digesta). Results from this study highlight how the built environment is a unique source of microbes to colonize fish mucus and furthermore how this can influence the fish health. Further studies can use this knowledge to engineer built environments to modulate fish microbiota for a beneficial phenotype.
Saengchan Senapin - One of the best experts on this subject based on the ideXlab platform.
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two year surveillance of tilapia lake virus tilv reveals its wide circulation in tilapia farms and Hatcheries from multiple districts of bangladesh
Journal of Fish Diseases, 2020Co-Authors: Partho Pratim Debnath, Jerome Delamaredeboutteville, Mona Dverdal Jansen, Kornsunee Phiwsaiya, Afsana Dalia, Abir Hasan, Saengchan SenapinAbstract:Tilapia lake virus (TiLV) is an emerging pathogen in aquaculture, reportedly affecting farmed tilapia in 16 countries across multiple continents. Following an early warning in 2017 that TiLV might be widespread, we executed a surveillance programme on tilapia grow-out farms and Hatcheries from 10 districts of Bangladesh in 2017 and 2019. Among farms experiencing unusual mortality, eight out of 11 farms tested positive for TiLV in 2017, and two out of seven tested positive in 2019. Investigation of asymptomatic broodstock collected from 16 tilapia Hatcheries revealed that six Hatcheries tested positive for TiLV. Representative samples subjected to histopathology confirmed pathognomonic lesions of syncytial hepatitis. We recovered three complete genomes of TiLV from infected fish, one from 2017 and two from 2019. Phylogenetic analyses based on both the concatenated coding sequences of 10 segments and only segment 1 consistently revealed that Bangladeshi TiLV isolates formed a unique cluster within Thai clade, suggesting a close genetic relation. In summary, this study revealed the circulation of TiLV in 10 farms and six Hatcheries located in eight districts of Bangladesh. We recommend continuing TiLV-targeted surveillance efforts to identify contaminated sources to minimize the countrywide spread and severity of TiLV infection.
Jongsheek Kim - One of the best experts on this subject based on the ideXlab platform.
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a taqman real time pcr assay for quantifying white spot syndrome virus wssv infections in wild broodstock and hatchery reared postlarvae of fleshy shrimp fenneropenaeus chinensis
Aquaculture, 2009Co-Authors: Inkwon Jang, Xianhong Meng, Hyungchul Seo, Yeongrok Cho, Bongrae Kim, Gopalakannan Ayyaru, Jongsheek KimAbstract:Abstract In the present study, a highly sensitive and specific TaqMan real-time PCR was developed to quantify white spot syndrome virus (WSSV) infections in wild broodstock and hatchery-reared postlarvae of fleshy shrimp, Fenneropenaeus chinensis . A total of 159 individuals of wild F. chinensis brooders from 3 locations were captured and 210 postlarvae (PL 1–8 ) were obtained from seven commercial Hatcheries in 2007 in South Korea. The WSSV infections in 3 broodstocks showed a wide range, from 0 to 2.28 × 10 6 (with a mean of 1.50 × 10 4 ) copies ng − 1 of DNA. Out of 159 brooders assayed, 39 (24.5%) were negative and 120 (75.5%) were positive; 153 (96.2%) showed less than 100 copies (mean 10.2 copies), 111 (69.8%) showed less than 10 copies and only 6 individuals (3.8%) showed high infections with a range of 2.36 × 10 2 to 2.28 × 10 6 copies ng − 1 of DNA. In 210 postlarvae, a range of 2.6 to 713.6 (with a mean of 220) copies g − 1 of DNA was observed. The mean WSSV copy number in the postlarvae was 7.9 × 10 5 , which was equivalent to 8.5 × 10 5 copies mg − 1 of postlarvae weight. A total of 87.1% of postlarvae were WSSV positive and except for two Hatcheries (H 4 and H 7 ), the postlarvae of all the other five Hatcheries were positive. Even postlarvae from the same hatchery, especially of Hatcheries H 4 and H 5 , showed a wide range of WSSV infection resulting in higher infections than other Hatcheries. There were 34.3% of the postlarvae assayed in the present study that showed very low infection, with less than 10 copies ng − 1 of DNA. Based on our results, it is recommended to pre-screen broodstock or larvae for selective breeding, stocking in production systems or stock enhancement.
Baskaralingam Vaseeharan - One of the best experts on this subject based on the ideXlab platform.
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in vitro susceptibility of antibiotics against vibrio spp and aeromonas spp isolated from penaeus monodon Hatcheries and ponds
International Journal of Antimicrobial Agents, 2005Co-Authors: Baskaralingam Vaseeharan, P Ramasamy, T Murugan, Jiannchu ChenAbstract:Susceptibility patterns to 16 different antibiotics were investigated against pathogenic Vibrio spp. and Aeromonas spp. isolated from shrimp culture Hatcheries and ponds in India. Thirteen species of Vibrio (N = 90) and two species of Aeromonas (N = 7) isolates were tested by agar disk diffusion. The results show that 100% of the isolates were resistant to ampicillin, and that 43.2% and 47.4% were sensitive to chlortetracycline and erythromycin, respectively. Susceptibility patterns of another 160 isolates belonging to the genera Vibrio and Aeromonas obtained from the water samples of shrimp Hatcheries and ponds were tested against six commonly used antibiotics. Results indicate that isolates from the Hatcheries were more resistant to antimicrobials than isolates from the ponds. The minimum inhibitory concentrations of five antibiotics against the different Vibrio spp. and Aeromonas spp. were determined. Ciprofloxacin was found to be the most effective in controlling the isolates from Hatcheries and ponds compared with the other antibiotics used in the study. Our results reveal that antibiotic-resistant bacteria are widespread in the shrimp culture Hatcheries and ponds in India. Potential risk to human health was not addressed in this study and remains to be elucidated.
Jj Minich - One of the best experts on this subject based on the ideXlab platform.
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microbial ecology of atlantic salmon salmo salar Hatcheries impacts of the built environment on fish mucosal microbiota
Applied and Environmental Microbiology, 2020Co-Authors: Jj Minich, Jp Bowman, Greg D Poore, Khattapan Jantawongsri, Colin Johnston, Kate Bowie, Rob Knight, B F Nowak, Eric E AllenAbstract:Successful rearing of fish in Hatcheries is critical for conservation, recreational fishing, commercial fishing through wild stock enhancements, and aquaculture production. Flow through (FT) Hatcheries require more water than Recirculating-Aquaculture-Systems (RAS) which enable up to 99% of water to be recycled thus significantly reducing environmental impacts. Here, we evaluated the biological and physical microbiome interactions of three Atlantic salmon Hatcheries (RAS n=2, FT n=1). Gill, skin, and digesta from six juvenile fish along with tank biofilms and water were sampled from tanks in each of the Hatcheries (60 fish across 10 tanks) to assess the built environment and mucosal microbiota using 16S rRNA gene sequencing. The water and tank biofilm had more microbial richness than fish mucus while skin and digesta from RAS fish had 2x the richness of FT fish. Body sites each had unique microbiomes (P IMPORTANCE Atlantic salmon, Salmo salar, is the most farmed marine fish worldwide with an annual production of 2,248 million metric tonnes in 2016. Salmon Hatcheries are increasingly changing from flow through towards RAS design to accommodate more control over production along with improved environmental sustainability due to lower impacts on water consumption. To date, microbiome studies on Hatcheries have focused either on the fish mucosal microbiota or the built environment microbiota, but have not combined the two to understand interactions. Our study evaluates how water and tank biofilm microbiota influences fish microbiota across three mucosal environments (gill, skin, and digesta). Results from this study highlight how the built environment is a unique source of microbes to colonize fish mucus and furthermore how this can influence fish health. Further studies can use this knowledge to engineer built environments to modulate fish microbiota for beneficial phenotypes.
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Microbial ecology of Atlantic salmon (Salmo salar) Hatcheries: impacts of the built environment on fish mucosal microbiota
'American Society for Microbiology', 2020Co-Authors: Jj Minich, Gd Poore, Jantawongsri K, Cj Johnston, Bowie K, Jp Bowman, Knight R, Nowak B, Ee AllenAbstract:Successful rearing of fish in Hatcheries is critical for conservation, recreational fishing, commercial fishing through wild stock enhancements, and aquaculture production. Flowthrough (FT) Hatcheries require more water than recirculating aquaculture systems (RAS), which enable up to 99% of their water to be recycled, thus significantly reducing environmental impacts. Here, we evaluated the biological and physical microbiome interactions of three Atlantic salmon Hatcheries (RAS n = 2, FT n = 1). Gill, skin, and digesta from six juvenile fish along with tank biofilms and water were sampled from tanks in each of the Hatcheries (60 fish across 10 tanks) to assess the built environment and mucosal microbiota using 16S rRNA gene sequencing. The water and tank biofilm had more microbial richness than fish mucus, while skin and digesta from RAS fish had 2 times the richness of FT fish. Body sites each had unique microbiomes (P P
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microbial ecology of atlantic salmon salmo salar Hatcheries impacts of the built environment on fish mucosal microbiota
bioRxiv, 2019Co-Authors: Jj Minich, Jp Bowman, Khattapan Jantawongsri, Colin Johnston, Kate Bowie, Rob Knight, B F Nowak, Eric E AllenAbstract:Successful rearing of fish in Hatcheries is critical for conservation, recreational fishing, and commercial fishing through wild stock enhancements, and aquaculture production. Flow through (FT) Hatcheries require more water than Recirculating-Aquaculture-Systems (RAS) which enable up to 99% of water to be recycled thus significantly reducing environmental impacts. Here, we evaluated the biological and physical microbiome interactions of the built environment of a hatchery from three Atl salmon Hatcheries (RAS n=2, FT n=1). Six juvenile fish were sampled from tanks in each of the Hatcheries for a total of 60 fish across 10 tanks. Water and tank side biofilm samples were collected from each of the tanks along with three salmon body sites (gill, skin, and digesta) to assess mucosal microbiota using 16S rRNA sequencing. The water and tank biofilm had more microbial richness than fish mucus while skin and digesta from RAS fish had 2x the richness of FT fish. Body sites each had unique microbial communities (P<0.001) and were influenced by the various hatchery systems (P<0.001) with RAS systems more similar. Water and especially tank biofilm richness was positively correlated with skin and digesta richness. Strikingly, the gill, skin and digesta communities were more similar to the origin tank biofilm vs. all other experimental tanks suggesting that the tank biofilm has a direct influence on fish-associated microbial communities. The results from this study provide evidence for a link between the tank microbiome and the fish microbiome with the skin microbiome as an important intermediate.nnIMPORTANCEAtlantic salmon, Salmo salar, is the most farmed marine fish worldwide with an annual production of 2,248 million metric tonnes in 2016. Salmon Hatcheries are increasingly changing from flow through towards RAS design to accommodate more control over production along with improved environmental sustainability due to lower impacts on water consumption. To date, microbiome studies on Hatcheries have focused either on the fish mucosal microbiota or the built environment microbiota, but have not combined the two to understand interactions. Our study evaluates how water and tank biofilm microbiota influences fish microbiota across three mucosal environments (gill, skin, and digesta). Results from this study highlight how the built environment is a unique source of microbes to colonize fish mucus and furthermore how this can influence the fish health. Further studies can use this knowledge to engineer built environments to modulate fish microbiota for a beneficial phenotype.
