Heart Edema

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Anna Gorąca - One of the best experts on this subject based on the ideXlab platform.

  • Influence of NADPH oxidase inhibition on oxidative stress parameters in rat Hearts
    Pharmacological Reports, 2013
    Co-Authors: Paulina Kleniewska, Marta Michalska, Anna Gorąca
    Abstract:

    Background The aim of this study was to assess whether apocynin, an nicotinamide adenine dinucleotide phosphate (NADPH) oxidase blocker, influences lipid peroxidation TBARS, hydrogen peroxide (H_2O_2) content, protein level, Heart Edema, tumor necrosis factor a (TNF-α) concentration or the glutathione redox system in Heart homogenates obtained from endothelin 1 (ET-1)-induced oxidative stress rats. Methods Experiments were carried out on adult male Wistar-Kyoto rats. The animals were divided into 4 groups: Group I: saline-treated control; Group II: saline followed by ET-1 (3 μg/kg b.w., iv); Group III: apocynin (5 mg/kg b.w., iv) administered half an hour before saline; Group IV: apocynin (5 mg/kg b.w., iv) administered half an hour before ET-1 (3 μg/kg b.w., iv). Results Injection of ET-1 alone showed a significant (p < 0.001) increase in thiobarbituric acid reactive substances (TBARS) and the hydrogen peroxide level (p < 0.01) vs. control, as well as a decrease (p < 0.001) in the GSH level. Apocynin significantly decreased TBARS (p < 0.001) and H_2O_2 (p < 0.05) level (vs. control) as well as improved protein level (p < 0.001) in the Heart. Apocynin also prevented ET-1-induced Heart Edema (p < 0.05). The presence of ET-1 increased the concentration of TNF-α (p < 0.05) while apocynin decreased it (p < 0.05). Our results indicate that ET-1 may induce oxidative stress in Heart tissue by reducing the GSH/GSSG ratio, stimulating lipid peroxidation and increasing TNF-α concentration. Apocynin diminished these measures of oxidative stress and TNF-α. Conclusion ET-1-induced formation of ROS in the Heart is at least partially regulated via NADPH oxidase.

  • Influence of NADPH oxidase inhibition on oxidative stress parameters in rat Hearts
    Pharmacological Reports, 2013
    Co-Authors: Paulina Kleniewska, Marta Michalska, Anna Gorąca
    Abstract:

    Abstract Background The aim of this study was to assess whether apocynin, an nicotinamide adenine dinucleotide phosphate (NADPH) oxidase blocker, influences lipid peroxidation TBARS, hydrogen peroxide (H 2 O 2 ) content, protein level, Heart Edema, tumor necrosis factor a (TNF-α) concentration or the glutathione redox system in Heart homogenates obtained from endothelin 1 (ET-1)-induced oxidative stress rats. Methods Experiments were carried out on adult male Wistar-Kyoto rats. The animals were divided into 4 groups: Group I: saline-treated control; Group II: saline followed by ET-1 (3 μg/kg b.w., iv ); Group III: apocynin (5 mg/kg b.w., iv ) administered half an hour before saline; Group IV: apocynin (5 mg/kg b.w., iv ) administered half an hour before ET-1 (3 μg/kg b.w., iv ). Results Injection of ET-1 alone showed a significant (p  vs. control, as well as a decrease (p  2 O 2 (p  vs . control) as well as improved protein level (p  Conclusion ET-1-induced formation of ROS in the Heart is at least partially regulated via NADPH oxidase.

Paulina Kleniewska - One of the best experts on this subject based on the ideXlab platform.

  • Influence of NADPH oxidase inhibition on oxidative stress parameters in rat Hearts
    Pharmacological Reports, 2013
    Co-Authors: Paulina Kleniewska, Marta Michalska, Anna Gorąca
    Abstract:

    Background The aim of this study was to assess whether apocynin, an nicotinamide adenine dinucleotide phosphate (NADPH) oxidase blocker, influences lipid peroxidation TBARS, hydrogen peroxide (H_2O_2) content, protein level, Heart Edema, tumor necrosis factor a (TNF-α) concentration or the glutathione redox system in Heart homogenates obtained from endothelin 1 (ET-1)-induced oxidative stress rats. Methods Experiments were carried out on adult male Wistar-Kyoto rats. The animals were divided into 4 groups: Group I: saline-treated control; Group II: saline followed by ET-1 (3 μg/kg b.w., iv); Group III: apocynin (5 mg/kg b.w., iv) administered half an hour before saline; Group IV: apocynin (5 mg/kg b.w., iv) administered half an hour before ET-1 (3 μg/kg b.w., iv). Results Injection of ET-1 alone showed a significant (p < 0.001) increase in thiobarbituric acid reactive substances (TBARS) and the hydrogen peroxide level (p < 0.01) vs. control, as well as a decrease (p < 0.001) in the GSH level. Apocynin significantly decreased TBARS (p < 0.001) and H_2O_2 (p < 0.05) level (vs. control) as well as improved protein level (p < 0.001) in the Heart. Apocynin also prevented ET-1-induced Heart Edema (p < 0.05). The presence of ET-1 increased the concentration of TNF-α (p < 0.05) while apocynin decreased it (p < 0.05). Our results indicate that ET-1 may induce oxidative stress in Heart tissue by reducing the GSH/GSSG ratio, stimulating lipid peroxidation and increasing TNF-α concentration. Apocynin diminished these measures of oxidative stress and TNF-α. Conclusion ET-1-induced formation of ROS in the Heart is at least partially regulated via NADPH oxidase.

  • Influence of NADPH oxidase inhibition on oxidative stress parameters in rat Hearts
    Pharmacological Reports, 2013
    Co-Authors: Paulina Kleniewska, Marta Michalska, Anna Gorąca
    Abstract:

    Abstract Background The aim of this study was to assess whether apocynin, an nicotinamide adenine dinucleotide phosphate (NADPH) oxidase blocker, influences lipid peroxidation TBARS, hydrogen peroxide (H 2 O 2 ) content, protein level, Heart Edema, tumor necrosis factor a (TNF-α) concentration or the glutathione redox system in Heart homogenates obtained from endothelin 1 (ET-1)-induced oxidative stress rats. Methods Experiments were carried out on adult male Wistar-Kyoto rats. The animals were divided into 4 groups: Group I: saline-treated control; Group II: saline followed by ET-1 (3 μg/kg b.w., iv ); Group III: apocynin (5 mg/kg b.w., iv ) administered half an hour before saline; Group IV: apocynin (5 mg/kg b.w., iv ) administered half an hour before ET-1 (3 μg/kg b.w., iv ). Results Injection of ET-1 alone showed a significant (p  vs. control, as well as a decrease (p  2 O 2 (p  vs . control) as well as improved protein level (p  Conclusion ET-1-induced formation of ROS in the Heart is at least partially regulated via NADPH oxidase.

Marta Michalska - One of the best experts on this subject based on the ideXlab platform.

  • Influence of NADPH oxidase inhibition on oxidative stress parameters in rat Hearts
    Pharmacological Reports, 2013
    Co-Authors: Paulina Kleniewska, Marta Michalska, Anna Gorąca
    Abstract:

    Background The aim of this study was to assess whether apocynin, an nicotinamide adenine dinucleotide phosphate (NADPH) oxidase blocker, influences lipid peroxidation TBARS, hydrogen peroxide (H_2O_2) content, protein level, Heart Edema, tumor necrosis factor a (TNF-α) concentration or the glutathione redox system in Heart homogenates obtained from endothelin 1 (ET-1)-induced oxidative stress rats. Methods Experiments were carried out on adult male Wistar-Kyoto rats. The animals were divided into 4 groups: Group I: saline-treated control; Group II: saline followed by ET-1 (3 μg/kg b.w., iv); Group III: apocynin (5 mg/kg b.w., iv) administered half an hour before saline; Group IV: apocynin (5 mg/kg b.w., iv) administered half an hour before ET-1 (3 μg/kg b.w., iv). Results Injection of ET-1 alone showed a significant (p < 0.001) increase in thiobarbituric acid reactive substances (TBARS) and the hydrogen peroxide level (p < 0.01) vs. control, as well as a decrease (p < 0.001) in the GSH level. Apocynin significantly decreased TBARS (p < 0.001) and H_2O_2 (p < 0.05) level (vs. control) as well as improved protein level (p < 0.001) in the Heart. Apocynin also prevented ET-1-induced Heart Edema (p < 0.05). The presence of ET-1 increased the concentration of TNF-α (p < 0.05) while apocynin decreased it (p < 0.05). Our results indicate that ET-1 may induce oxidative stress in Heart tissue by reducing the GSH/GSSG ratio, stimulating lipid peroxidation and increasing TNF-α concentration. Apocynin diminished these measures of oxidative stress and TNF-α. Conclusion ET-1-induced formation of ROS in the Heart is at least partially regulated via NADPH oxidase.

  • Influence of NADPH oxidase inhibition on oxidative stress parameters in rat Hearts
    Pharmacological Reports, 2013
    Co-Authors: Paulina Kleniewska, Marta Michalska, Anna Gorąca
    Abstract:

    Abstract Background The aim of this study was to assess whether apocynin, an nicotinamide adenine dinucleotide phosphate (NADPH) oxidase blocker, influences lipid peroxidation TBARS, hydrogen peroxide (H 2 O 2 ) content, protein level, Heart Edema, tumor necrosis factor a (TNF-α) concentration or the glutathione redox system in Heart homogenates obtained from endothelin 1 (ET-1)-induced oxidative stress rats. Methods Experiments were carried out on adult male Wistar-Kyoto rats. The animals were divided into 4 groups: Group I: saline-treated control; Group II: saline followed by ET-1 (3 μg/kg b.w., iv ); Group III: apocynin (5 mg/kg b.w., iv ) administered half an hour before saline; Group IV: apocynin (5 mg/kg b.w., iv ) administered half an hour before ET-1 (3 μg/kg b.w., iv ). Results Injection of ET-1 alone showed a significant (p  vs. control, as well as a decrease (p  2 O 2 (p  vs . control) as well as improved protein level (p  Conclusion ET-1-induced formation of ROS in the Heart is at least partially regulated via NADPH oxidase.

Nguyen Dinh Thang - One of the best experts on this subject based on the ideXlab platform.

  • Toxicological and melanin synthesis effects of Polygonum multiflorum root extracts on zebrafish embryos and human melanocytes
    Biomedical Research and Therapy, 2016
    Co-Authors: Dang Thi Hoai Thanh, Nguyen Lai Thanh, Nguyen Dinh Thang
    Abstract:

    Polygonum multiflorum (PM) has been commmonly used as folk medicine for treatment of various conditions, such as early graying of hair in humans. However, there have been limited studies which have evaluated the toxicological and biological effects of PM in vitro as well as in vivo. In this study, PM root extracts in ethyl acetate (PM-E) and in distilled water (PM-W) were examined for their effects on the development of teratogenic defects/deaths. Additionally, they were evaluated for their effects on melanin formation in human melanocytes and pigmentation in embryos/larvae of wild type strain AB zebrafish (Danio rerio). Our results showed that PM root extracts at concentrations of 40 mg/L and 105 mg/L induced the development of teratogenic defects, including yolk sac Edema (or Heart Edema), hemovascular defects, necrosis and abnormal trunk in zebrafish embryos at 4 days post fertilization; teratogenic indexes (TIs) were 1.43 and 0.63 for ethyl acetate extract and distilled water extract, respectively. Our results also demonstrated that PM-W significantly increased the pigmentation level of embryos/larvae and induced melanin formation in human melanocytes. The amount of melanin in PM-W-exposed embryos/larvae was 2.2-fold and 1.71-fold greater than those in the control embryos/larvae and control melanocytes, respectively. Our study also showed that the increased level of pigmentation in PM-W embryos/larvae or melanin biosynthesis in melanocytes were both regulated by activation of tyrosinase. Conclusively, our study suggests that PM root extracts could be used as potential agents for treatment of early hair graying as well as various other diseases related to loss of pigmentation. However, these PM root extracts may also have some negative effects on embryos; therefore it should be careful when using for women during pregnancy.

  • Toxicological and melanin synthesis effects of Polygonum multiflorum root extracts on zebrafish embryos and human melanocytes
    Biomedical Research and Therapy, 2016
    Co-Authors: Dang Thi Hoai Thanh, Nguyen Lai Thanh, Nguyen Dinh Thang
    Abstract:

    Introduction: Polygonum multiflorum (PM) has been commonly used as folk medicine for treatment of various conditions, such as early graying of hair in humans. However, there have been limited studies which have evaluated the toxicological and biological effects of PM in vitro as well as in vivo. In this study, PM root extracts in ethyl acetate (PM-E) and in distilled water (PM-W) were examined for their effects on the development of teratogenic defects/deaths. Additionally, they were evaluated for their effects on melanin formation in human melanocytes and pigmentation in embryos/larvae of wild type strain AB zebrafish (Danio rerio). Methods: Phytochemicals in PM roots were extracted in several solvents with increasing in their polarities. Chemical exposure tests were performed to investigate the toxic effects of PM root extracts on Zerbrafish embryos/larvae. Total melanins formed in zebrafish embryos/larvae as well as in human melanocytes were measured by spectrophotometer using dual wavelengths of 415 and 400 nm. Gene expressions were analyzed by realtime PCR. All experiments were independently repeated three times and statistical analyzed. Results: Our results showed that PM root extracts at concentrations of 40 mg/L and 105 mg/L induced the development of teratogenic defects, including yolk sac Edema (or Heart Edema), hemovascular defects, necrosis and abnormal trunk in zebrafish embryos at 4 days post fertilization; teratogenic indexes (TIs) were 1.43 and 0.63 for ethyl acetate extract and distilled water extract, respectively. Our results also demonstrated that PM-W significantly increased the pigmentation level of embryos/larvae and induced melanin formation in human melanocytes. The amount of melanin in PM-W-exposed embryos/larvae was 2.2-fold and 1.71-fold greater than those in the control embryos/larvae and control melanocytes, respectively. Conclusion: Our study also showed that the increased level of pigmentation in PM-W embryos/larvae or melanin biosynthesis in melanocytes were both regulated by activation of tyrosinase. Conclusively, our study suggests that PM root extracts could be used as potential agents for treatment of early hair graying as well as various other diseases related to loss of pigmentation. However, these PM root extracts may also have some negative effects on embryos; therefore it should be careful when using for women during pregnancy.

Dang Thi Hoai Thanh - One of the best experts on this subject based on the ideXlab platform.

  • Toxicological and melanin synthesis effects of Polygonum multiflorum root extracts on zebrafish embryos and human melanocytes
    Biomedical Research and Therapy, 2016
    Co-Authors: Dang Thi Hoai Thanh, Nguyen Lai Thanh, Nguyen Dinh Thang
    Abstract:

    Polygonum multiflorum (PM) has been commmonly used as folk medicine for treatment of various conditions, such as early graying of hair in humans. However, there have been limited studies which have evaluated the toxicological and biological effects of PM in vitro as well as in vivo. In this study, PM root extracts in ethyl acetate (PM-E) and in distilled water (PM-W) were examined for their effects on the development of teratogenic defects/deaths. Additionally, they were evaluated for their effects on melanin formation in human melanocytes and pigmentation in embryos/larvae of wild type strain AB zebrafish (Danio rerio). Our results showed that PM root extracts at concentrations of 40 mg/L and 105 mg/L induced the development of teratogenic defects, including yolk sac Edema (or Heart Edema), hemovascular defects, necrosis and abnormal trunk in zebrafish embryos at 4 days post fertilization; teratogenic indexes (TIs) were 1.43 and 0.63 for ethyl acetate extract and distilled water extract, respectively. Our results also demonstrated that PM-W significantly increased the pigmentation level of embryos/larvae and induced melanin formation in human melanocytes. The amount of melanin in PM-W-exposed embryos/larvae was 2.2-fold and 1.71-fold greater than those in the control embryos/larvae and control melanocytes, respectively. Our study also showed that the increased level of pigmentation in PM-W embryos/larvae or melanin biosynthesis in melanocytes were both regulated by activation of tyrosinase. Conclusively, our study suggests that PM root extracts could be used as potential agents for treatment of early hair graying as well as various other diseases related to loss of pigmentation. However, these PM root extracts may also have some negative effects on embryos; therefore it should be careful when using for women during pregnancy.

  • Toxicological and melanin synthesis effects of Polygonum multiflorum root extracts on zebrafish embryos and human melanocytes
    Biomedical Research and Therapy, 2016
    Co-Authors: Dang Thi Hoai Thanh, Nguyen Lai Thanh, Nguyen Dinh Thang
    Abstract:

    Introduction: Polygonum multiflorum (PM) has been commonly used as folk medicine for treatment of various conditions, such as early graying of hair in humans. However, there have been limited studies which have evaluated the toxicological and biological effects of PM in vitro as well as in vivo. In this study, PM root extracts in ethyl acetate (PM-E) and in distilled water (PM-W) were examined for their effects on the development of teratogenic defects/deaths. Additionally, they were evaluated for their effects on melanin formation in human melanocytes and pigmentation in embryos/larvae of wild type strain AB zebrafish (Danio rerio). Methods: Phytochemicals in PM roots were extracted in several solvents with increasing in their polarities. Chemical exposure tests were performed to investigate the toxic effects of PM root extracts on Zerbrafish embryos/larvae. Total melanins formed in zebrafish embryos/larvae as well as in human melanocytes were measured by spectrophotometer using dual wavelengths of 415 and 400 nm. Gene expressions were analyzed by realtime PCR. All experiments were independently repeated three times and statistical analyzed. Results: Our results showed that PM root extracts at concentrations of 40 mg/L and 105 mg/L induced the development of teratogenic defects, including yolk sac Edema (or Heart Edema), hemovascular defects, necrosis and abnormal trunk in zebrafish embryos at 4 days post fertilization; teratogenic indexes (TIs) were 1.43 and 0.63 for ethyl acetate extract and distilled water extract, respectively. Our results also demonstrated that PM-W significantly increased the pigmentation level of embryos/larvae and induced melanin formation in human melanocytes. The amount of melanin in PM-W-exposed embryos/larvae was 2.2-fold and 1.71-fold greater than those in the control embryos/larvae and control melanocytes, respectively. Conclusion: Our study also showed that the increased level of pigmentation in PM-W embryos/larvae or melanin biosynthesis in melanocytes were both regulated by activation of tyrosinase. Conclusively, our study suggests that PM root extracts could be used as potential agents for treatment of early hair graying as well as various other diseases related to loss of pigmentation. However, these PM root extracts may also have some negative effects on embryos; therefore it should be careful when using for women during pregnancy.

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