The Experts below are selected from a list of 244623 Experts worldwide ranked by ideXlab platform
Hideaki Higashino - One of the best experts on this subject based on the ideXlab platform.
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Reduction of Cardiac Norepinephrine Improves Postischemic Heart Function in Stroke-prone Spontaneously Hypertensive Rats
Journal of cardiovascular pharmacology, 2001Co-Authors: Hong Chen, Kana Maeda, Hideaki Higashino, Zhiwei Zhang, Yoshio Ohta, Zhong Wang, Wen-jun YuanAbstract:Summary: Although mammalian ventricle is richly supplied with adrenergic nerves, endogenous norepinephrine is not essential to the intrinsic contractility of the normal Heart. However, it is not clear whether acute changes in cardiac norepinephrine could alter Heart Function in genetically hypertensive rats. The purpose of this study was to examine the effect of cardiac norepinephrine reduction on basal and postischemic Heart Function in stroke-prone spontaneously hypertensive rats (SHRSPs) using an isolated working Heart preparation. Hypertrophied Hearts of SHRSPs showed higher cardiac norepinephrine content and impaired Heart Function at 4 months of age as compared with normal Wistar-Kyoto rats. Poor postischemic recovery of Heart Function observed in SHRSPs was accompanied by large amounts of coronary norepinephrine overflow. Cardiac norepinephrine reduction or depletion did not affect basal Heart Function in SHRSPs. Considerable reduction in cardiac norepinephrine with acute reserpine injection (5 mg/kg) in SHRSPs significantly improved postischemic recovery of cardiac output, coronary flow, and rate-pressure product. However, complete norepinephrine depletion with reserpine (10 mg/kg) was detrimental to myocardial automaticity and limited the postischemic recovery of systolic Function in the hypertrophied Hearts. These results suggest that acute reduction in cardiac norepinephrine may be of potential therapeutic importance to postischemic dysFunction in the hypertrophied Hearts.
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Impaired Heart Function and noradrenaline release after ischaemia in stroke-prone spontaneously hypertensive rats.
Clinical and experimental pharmacology & physiology, 2000Co-Authors: Hong Chen, Makiko Azuma, Kana Maeda, Noriyoshi Kajimoto, Hideaki HigashinoAbstract:SUMMARY 1. Stroke-prone spontaneously hypertensive rats (SHRSP) are a strain of rat that exhibit severely high blood pressure and stroke attacks at an early age, but their Heart Function in vitro has seldom been studied in detail. Although the activity of the sympathetic nervous system is known to increase after myocardial ischaemia, there is little information about the cardiac release of noradrenaline (NA) associated with Heart Function after ischaemia in SHRSP. The aim of the present study was to examine Heart Function and cardiac NA release after ischaemia in SHRSP. 2. Isolated Hearts of 4- and 8-month-old SHRSP and age-matched Wistar-Kyoto (WKY) rats were perfused in a working Heart preparation and were subjected to 30 min ischaemia followed by 30 min reperfusion. Heart Function and coronary flow were monitored throughout the experiment. Coronary effluent was collected for determination of NA using high- performance liquid chromatography coupled with electrochemical detection. 3. Under baseline conditions, cardiac output of 4-month-old SHRSP was slightly but significantly decreased compared with that of WKY rats (P < 0.05), although coronary flow was maintained normally at this age. Eight-month-old SHRSP showed a further impairment of systolic Heart Function, with lower coronary flow and higher coronary vascular resistance under baseline conditions. Elevated left ventricular end-diastolic pressure was evident in SHRSP at both ages before ischaemia. Heart Function was severely damaged after 30 min global ischaemia in SHRSP from both age groups. Stroke-prone spontaneously hypertensive rats also showed lower coronary flow and higher coronary vascular resistance during reperfusion. 4. Coronary NA was not detectable in WKY rats or SHRSP at 4 months of age under baseline conditions. In 8-month-old SHRSP, pre-ischaemic NA release was significantly higher than that in age-matched WKY rat controls. The concentration of NA in the coronary effluent of SHRSP during reperfusion was also significantly higher than that of WKY rats at both ages. 5. These data demonstrate that SHRSP have early impairment of both systolic and diastolic Heart Function compared with WKY rats. Severe damage of Heart Function and coronary flow after ischaemia in SHRSP was accompanied with an increased release of NA, which may play a harmful role in Heart Function impairment in SHRSP after ischaemia.
Hong Chen - One of the best experts on this subject based on the ideXlab platform.
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Reduction of Cardiac Norepinephrine Improves Postischemic Heart Function in Stroke-prone Spontaneously Hypertensive Rats
Journal of cardiovascular pharmacology, 2001Co-Authors: Hong Chen, Kana Maeda, Hideaki Higashino, Zhiwei Zhang, Yoshio Ohta, Zhong Wang, Wen-jun YuanAbstract:Summary: Although mammalian ventricle is richly supplied with adrenergic nerves, endogenous norepinephrine is not essential to the intrinsic contractility of the normal Heart. However, it is not clear whether acute changes in cardiac norepinephrine could alter Heart Function in genetically hypertensive rats. The purpose of this study was to examine the effect of cardiac norepinephrine reduction on basal and postischemic Heart Function in stroke-prone spontaneously hypertensive rats (SHRSPs) using an isolated working Heart preparation. Hypertrophied Hearts of SHRSPs showed higher cardiac norepinephrine content and impaired Heart Function at 4 months of age as compared with normal Wistar-Kyoto rats. Poor postischemic recovery of Heart Function observed in SHRSPs was accompanied by large amounts of coronary norepinephrine overflow. Cardiac norepinephrine reduction or depletion did not affect basal Heart Function in SHRSPs. Considerable reduction in cardiac norepinephrine with acute reserpine injection (5 mg/kg) in SHRSPs significantly improved postischemic recovery of cardiac output, coronary flow, and rate-pressure product. However, complete norepinephrine depletion with reserpine (10 mg/kg) was detrimental to myocardial automaticity and limited the postischemic recovery of systolic Function in the hypertrophied Hearts. These results suggest that acute reduction in cardiac norepinephrine may be of potential therapeutic importance to postischemic dysFunction in the hypertrophied Hearts.
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Impaired Heart Function and noradrenaline release after ischaemia in stroke-prone spontaneously hypertensive rats.
Clinical and experimental pharmacology & physiology, 2000Co-Authors: Hong Chen, Makiko Azuma, Kana Maeda, Noriyoshi Kajimoto, Hideaki HigashinoAbstract:SUMMARY 1. Stroke-prone spontaneously hypertensive rats (SHRSP) are a strain of rat that exhibit severely high blood pressure and stroke attacks at an early age, but their Heart Function in vitro has seldom been studied in detail. Although the activity of the sympathetic nervous system is known to increase after myocardial ischaemia, there is little information about the cardiac release of noradrenaline (NA) associated with Heart Function after ischaemia in SHRSP. The aim of the present study was to examine Heart Function and cardiac NA release after ischaemia in SHRSP. 2. Isolated Hearts of 4- and 8-month-old SHRSP and age-matched Wistar-Kyoto (WKY) rats were perfused in a working Heart preparation and were subjected to 30 min ischaemia followed by 30 min reperfusion. Heart Function and coronary flow were monitored throughout the experiment. Coronary effluent was collected for determination of NA using high- performance liquid chromatography coupled with electrochemical detection. 3. Under baseline conditions, cardiac output of 4-month-old SHRSP was slightly but significantly decreased compared with that of WKY rats (P < 0.05), although coronary flow was maintained normally at this age. Eight-month-old SHRSP showed a further impairment of systolic Heart Function, with lower coronary flow and higher coronary vascular resistance under baseline conditions. Elevated left ventricular end-diastolic pressure was evident in SHRSP at both ages before ischaemia. Heart Function was severely damaged after 30 min global ischaemia in SHRSP from both age groups. Stroke-prone spontaneously hypertensive rats also showed lower coronary flow and higher coronary vascular resistance during reperfusion. 4. Coronary NA was not detectable in WKY rats or SHRSP at 4 months of age under baseline conditions. In 8-month-old SHRSP, pre-ischaemic NA release was significantly higher than that in age-matched WKY rat controls. The concentration of NA in the coronary effluent of SHRSP during reperfusion was also significantly higher than that of WKY rats at both ages. 5. These data demonstrate that SHRSP have early impairment of both systolic and diastolic Heart Function compared with WKY rats. Severe damage of Heart Function and coronary flow after ischaemia in SHRSP was accompanied with an increased release of NA, which may play a harmful role in Heart Function impairment in SHRSP after ischaemia.
Kana Maeda - One of the best experts on this subject based on the ideXlab platform.
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Reduction of Cardiac Norepinephrine Improves Postischemic Heart Function in Stroke-prone Spontaneously Hypertensive Rats
Journal of cardiovascular pharmacology, 2001Co-Authors: Hong Chen, Kana Maeda, Hideaki Higashino, Zhiwei Zhang, Yoshio Ohta, Zhong Wang, Wen-jun YuanAbstract:Summary: Although mammalian ventricle is richly supplied with adrenergic nerves, endogenous norepinephrine is not essential to the intrinsic contractility of the normal Heart. However, it is not clear whether acute changes in cardiac norepinephrine could alter Heart Function in genetically hypertensive rats. The purpose of this study was to examine the effect of cardiac norepinephrine reduction on basal and postischemic Heart Function in stroke-prone spontaneously hypertensive rats (SHRSPs) using an isolated working Heart preparation. Hypertrophied Hearts of SHRSPs showed higher cardiac norepinephrine content and impaired Heart Function at 4 months of age as compared with normal Wistar-Kyoto rats. Poor postischemic recovery of Heart Function observed in SHRSPs was accompanied by large amounts of coronary norepinephrine overflow. Cardiac norepinephrine reduction or depletion did not affect basal Heart Function in SHRSPs. Considerable reduction in cardiac norepinephrine with acute reserpine injection (5 mg/kg) in SHRSPs significantly improved postischemic recovery of cardiac output, coronary flow, and rate-pressure product. However, complete norepinephrine depletion with reserpine (10 mg/kg) was detrimental to myocardial automaticity and limited the postischemic recovery of systolic Function in the hypertrophied Hearts. These results suggest that acute reduction in cardiac norepinephrine may be of potential therapeutic importance to postischemic dysFunction in the hypertrophied Hearts.
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Impaired Heart Function and noradrenaline release after ischaemia in stroke-prone spontaneously hypertensive rats.
Clinical and experimental pharmacology & physiology, 2000Co-Authors: Hong Chen, Makiko Azuma, Kana Maeda, Noriyoshi Kajimoto, Hideaki HigashinoAbstract:SUMMARY 1. Stroke-prone spontaneously hypertensive rats (SHRSP) are a strain of rat that exhibit severely high blood pressure and stroke attacks at an early age, but their Heart Function in vitro has seldom been studied in detail. Although the activity of the sympathetic nervous system is known to increase after myocardial ischaemia, there is little information about the cardiac release of noradrenaline (NA) associated with Heart Function after ischaemia in SHRSP. The aim of the present study was to examine Heart Function and cardiac NA release after ischaemia in SHRSP. 2. Isolated Hearts of 4- and 8-month-old SHRSP and age-matched Wistar-Kyoto (WKY) rats were perfused in a working Heart preparation and were subjected to 30 min ischaemia followed by 30 min reperfusion. Heart Function and coronary flow were monitored throughout the experiment. Coronary effluent was collected for determination of NA using high- performance liquid chromatography coupled with electrochemical detection. 3. Under baseline conditions, cardiac output of 4-month-old SHRSP was slightly but significantly decreased compared with that of WKY rats (P < 0.05), although coronary flow was maintained normally at this age. Eight-month-old SHRSP showed a further impairment of systolic Heart Function, with lower coronary flow and higher coronary vascular resistance under baseline conditions. Elevated left ventricular end-diastolic pressure was evident in SHRSP at both ages before ischaemia. Heart Function was severely damaged after 30 min global ischaemia in SHRSP from both age groups. Stroke-prone spontaneously hypertensive rats also showed lower coronary flow and higher coronary vascular resistance during reperfusion. 4. Coronary NA was not detectable in WKY rats or SHRSP at 4 months of age under baseline conditions. In 8-month-old SHRSP, pre-ischaemic NA release was significantly higher than that in age-matched WKY rat controls. The concentration of NA in the coronary effluent of SHRSP during reperfusion was also significantly higher than that of WKY rats at both ages. 5. These data demonstrate that SHRSP have early impairment of both systolic and diastolic Heart Function compared with WKY rats. Severe damage of Heart Function and coronary flow after ischaemia in SHRSP was accompanied with an increased release of NA, which may play a harmful role in Heart Function impairment in SHRSP after ischaemia.
Wengong Wang - One of the best experts on this subject based on the ideXlab platform.
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the g4 resolvase rhau modulates mrna translation and stability to sustain postnatal Heart Function and regeneration
Journal of Biological Chemistry, 2021Co-Authors: Mingyang Jiang, Ke Zhao, Xinyi Huang, Yingchao Shi, Yunyun Yue, Junwei Nie, Wengong Wang, Zhongzhou YangAbstract:Post-transcriptional regulation of mRNA translation and stability is primarily achieved by RNA-binding proteins, which are of increasing importance for Heart Function. Furthermore, G-quadruplex (G4) and G4 resolvase activity are involved in a variety of biological processes. However, the role of G4 resolvase activity in Heart Function remains unknown. The present study aims to investigate the role of RNA helicase associated with adenylate- and uridylate-rich element (RHAU), an RNA-binding protein with G4 resolvase activity in postnatal Heart Function through deletion of Rhau in the cardiomyocytes of postnatal mice. RHAU-deficient mice displayed progressive pathological remodeling leading to Heart failure and mortality and impaired neonatal Heart regeneration. RHAU ablation reduced the protein levels but enhanced mRNA levels of Yap1 and Hexim1 that are important regulators for Heart development and postnatal Heart Function. Furthermore, RHAU was found to associate with both the 5' and 3' UTRs of these genes to destabilize mRNA and enhance translation. Thus, we have demonstrated the important Functions of RHAU in the dual regulation of mRNA translation and stability, which is vital for Heart physiology.
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The G4 resolvase RHAU modulates mRNA translation and stability to sustain postnatal Heart Function and regeneration.
The Journal of biological chemistry, 2020Co-Authors: Mingyang Jiang, Ke Zhao, Xinyi Huang, Yingchao Shi, Yunyun Yue, Junwei Nie, Wengong WangAbstract:Post-transcriptional regulation of mRNA translation and stability is primarily achieved by RNA binding proteins (RBPs), which is of increasing importance for Heart Function. Furthermore, G-quadruplex (G4) and G4 resolvase activity are involved in a variety of biological processes. However, the role of G4 resolvase activity in Heart Function remains unknown. The present study aims to investigate the role of RHAU, an RBP with G4 resolvase activity in postnatal Heart Function through deletion of Rhau in the cardiomyocytes of postnatal mice. RHAU-deficient mice displayed progressive pathological remodeling leading to Heart failure and mortality, and impaired neonatal Heart regeneration. RHAU ablation reduced the protein levels but enhanced mRNA levels of Yap1 and Hexim1 that are important regulators for Heart development and postnatal Heart Function. Furthermore, RHAU was found to associate with both the 5'- and 3'- UTRs of these genes to destabilize mRNA but to enhance translation. Thus, we have demonstrated the important Functions of RHAU in the dual regulation of mRNA translation and stability, which is vital for Heart physiology.
Mingyang Jiang - One of the best experts on this subject based on the ideXlab platform.
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the g4 resolvase rhau modulates mrna translation and stability to sustain postnatal Heart Function and regeneration
Journal of Biological Chemistry, 2021Co-Authors: Mingyang Jiang, Ke Zhao, Xinyi Huang, Yingchao Shi, Yunyun Yue, Junwei Nie, Wengong Wang, Zhongzhou YangAbstract:Post-transcriptional regulation of mRNA translation and stability is primarily achieved by RNA-binding proteins, which are of increasing importance for Heart Function. Furthermore, G-quadruplex (G4) and G4 resolvase activity are involved in a variety of biological processes. However, the role of G4 resolvase activity in Heart Function remains unknown. The present study aims to investigate the role of RNA helicase associated with adenylate- and uridylate-rich element (RHAU), an RNA-binding protein with G4 resolvase activity in postnatal Heart Function through deletion of Rhau in the cardiomyocytes of postnatal mice. RHAU-deficient mice displayed progressive pathological remodeling leading to Heart failure and mortality and impaired neonatal Heart regeneration. RHAU ablation reduced the protein levels but enhanced mRNA levels of Yap1 and Hexim1 that are important regulators for Heart development and postnatal Heart Function. Furthermore, RHAU was found to associate with both the 5' and 3' UTRs of these genes to destabilize mRNA and enhance translation. Thus, we have demonstrated the important Functions of RHAU in the dual regulation of mRNA translation and stability, which is vital for Heart physiology.
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The G4 resolvase RHAU modulates mRNA translation and stability to sustain postnatal Heart Function and regeneration.
The Journal of biological chemistry, 2020Co-Authors: Mingyang Jiang, Ke Zhao, Xinyi Huang, Yingchao Shi, Yunyun Yue, Junwei Nie, Wengong WangAbstract:Post-transcriptional regulation of mRNA translation and stability is primarily achieved by RNA binding proteins (RBPs), which is of increasing importance for Heart Function. Furthermore, G-quadruplex (G4) and G4 resolvase activity are involved in a variety of biological processes. However, the role of G4 resolvase activity in Heart Function remains unknown. The present study aims to investigate the role of RHAU, an RBP with G4 resolvase activity in postnatal Heart Function through deletion of Rhau in the cardiomyocytes of postnatal mice. RHAU-deficient mice displayed progressive pathological remodeling leading to Heart failure and mortality, and impaired neonatal Heart regeneration. RHAU ablation reduced the protein levels but enhanced mRNA levels of Yap1 and Hexim1 that are important regulators for Heart development and postnatal Heart Function. Furthermore, RHAU was found to associate with both the 5'- and 3'- UTRs of these genes to destabilize mRNA but to enhance translation. Thus, we have demonstrated the important Functions of RHAU in the dual regulation of mRNA translation and stability, which is vital for Heart physiology.
