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  • phytochemical investigation of labdane diterpenes from the rhizomes of Hedychium spicatum and their cytotoxic activity
    Bioorganic & Medicinal Chemistry Letters, 2009
    Co-Authors: Prabhakar P Reddy, B S Sastry, Madhusudana J Rao, Ranga R Rao, J Shashidhar, Suresh K Babu
    Abstract:

    A comprehensive reinvestigation of chemical constituents from the rhizomes of Hedychium spicatum led to the isolation of two new labdane-type diterpene (1, 2), together with six known compounds (3-8). Their structures were established on the basis of extensive spectroscopic (IR, MS, 2D NMR) data analysis and by comparison with the spectroscopic data reported in the literature. In addition, all the isolates were tested for their cytotoxicity against the THP-1 (human acute monocytic leukemia), HL-60 (human promyelocytic leukemia), A-375 (human malignant melanoma) and A-549 (human lung carcinoma) cancerous cell lines.

  • new labdane diterpenes as intestinal α glucosidase inhibitor from antihyperglycemic extract of Hedychium spicatum ham ex smith rhizomes
    Bioorganic & Medicinal Chemistry Letters, 2009
    Co-Authors: Prabhakar P Reddy, Ashok K Tiwari, Ranga R Rao, K Madhusudhana, Rama Subba V Rao, Amtul Z Ali, Suresh K Babu, Madhusudana J Rao
    Abstract:

    Abstract Phytochemical investigation of antihyperglycemic extract of rhizomes of Hedychium spicatum led to the isolation of two new labdane type diterpenes 2, 3 along with seven known compounds (1, 4–9). Their structures were established on the basis of NMR (1D and 2D) and mass spectroscopic analysis. The new compound 2 displayed strong intestinal α-glucosidase inhibitory activity. Other compounds also displayed varying degree of intestinal α-glucosidase inhibitory potential.

  • two new cytotoxic diterpenes from the rhizomes of Hedychium spicatum
    Bioorganic & Medicinal Chemistry Letters, 2009
    Co-Authors: Prabhakar P Reddy, Ranga R Rao, Suresh K Babu, K Rekha, J Shashidhar, G Shashikiran, Vijaya V Lakshmi, Madhusudana J Rao
    Abstract:

    Phytochemical investigation of CHCl3 extract of the rhizomes of Hedychium spicatum led to the isolation of two new labdane-type diterpenes, compounds 1 and 2 along with five known compounds (3–7). Their structures were established on the basis of NMR (1D and 2D) and mass spectroscopic analysis. In addition, all the isolates were tested for their cytotoxicity against the Colo-205 (Colo-cancer), A-431 (skin cancer), MCF-7 (breast cancer), A-549 (lung cancer) and Chinese hamster ovary cells (CHO). Two new compounds 1 and 2 were shown good cytotoxic activity.

  • a new convenient method for quantitative analysis of hedychenone an anti inflammatory compound in the rhizomes of Hedychium spicatum buch hem
    Jpc-journal of Planar Chromatography-modern Tlc, 2007
    Co-Authors: P V Srinivas, Sekar Anubala, V U M Sarma, B S Sastry, Madhusudana J Rao
    Abstract:

    Plants of the Hedychium genus are perennial rhizomatus plants belonging to the Zingiberaceae family. Extracts of Zingiberacaea have long been used in traditional medicine. A simple, precise, and convenient HPTLC method has been established for analysis of hedychenone, the major marker compound extracted from the rhizomes of Hedychium spicatum (Buch-Hem). Chromatography was performed on silica gel 60F 254 plates with ethyl acetate-hexane, 20 + 80 ( v/v ), as mobile phase. Detection and quantification were performed densitometrically at λ max = 254 nm with hedychenone as external standard. The method is characterized by high sensitivity and linearity over a wide range of concentrations.