The Experts below are selected from a list of 120 Experts worldwide ranked by ideXlab platform
Sangwoo Lee - One of the best experts on this subject based on the ideXlab platform.
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Sound Packing DNA: packing open circular DNA with low-intensity ultrasound
Scientific Reports, 2015Co-Authors: Donghee Park, Bong-kwang Jung, Hyunjin Park, Hyungbeen Lee, Gyudo Lee, Jingam Park, Unchul Shin, Jong Ho Won, Jin Woo Chang, Sangwoo LeeAbstract:Supercoiling DNA (folding DNA into a more compact molecule) from open circular Forms requires significant bending energy. The double Helix is coiled into a higher order Helix Form; thus it occupies a smaller footprint. Compact packing of DNA is essential to improve the efficiency of gene delivery, which has broad implications in biology and pharmaceutical research. Here we show that low-intensity pulsed ultrasound can pack open circular DNA into supercoil Form. Plasmid DNA subjected to 5.4 mW/cm^2 intensity ultrasound showed significant (p-values
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sound packing dna packing open circular dna with low intensity ultrasound
Scientific Reports, 2015Co-Authors: Donghee Park, Bong-kwang Jung, Hyunjin Park, Hyungbeen Lee, Gyudo Lee, Jingam Park, Unchul Shin, Jong Ho Won, Jin Woo Chang, Sangwoo LeeAbstract:Supercoiling DNA (folding DNA into a more compact molecule) from open circular Forms requires significant bending energy. The double Helix is coiled into a higher order Helix Form; thus it occupies a smaller footprint. Compact packing of DNA is essential to improve the efficiency of gene delivery, which has broad implications in biology and pharmaceutical research. Here we show that low-intensity pulsed ultrasound can pack open circular DNA into supercoil Form. Plasmid DNA subjected to 5.4 mW/cm2 intensity ultrasound showed significant (p-values <0.001) supercoiling compared to DNA without exposure to ultrasound. Radiation force induced from ultrasound and dragging force from the fluid are believed to be the main factors that cause supercoiling. This study provides the first evidence to show that low-intensity ultrasound can directly alter DNA topology. We anticipate our results to be a starting point for improved non-viral gene delivery.
Susumu Okazaki - One of the best experts on this subject based on the ideXlab platform.
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all atom molecular dynamics study of a spherical micelle composed of n acetylated poly ethylene glycol poly gamma benzyl l glutamate block copolymers a potential carrier of drug delivery systems for cancer
Journal of Physical Chemistry B, 2009Co-Authors: Hiroshi Kuramochi, Yoshimichi Andoh, Noriyuki Yoshii, Susumu OkazakiAbstract:An all-atom molecular dynamics simulation of a spherical micelle composed of amphiphilic N-acetylated poly(ethylene glycol)−poly(γ-benzyl l-glutamate) (PEG−PBLG-Ac) block copolymers was perFormed in aqueous solution at 298.15 K and 1 atm. Such copolymers have received considerable attention as carriers in drug delivery systems. In this study, we used copolymers consisting of 11 EG units and 9 BLG units as models. Starting from the copolymers arranged spherically, the calculation predicted an equilibrium state consisting of a slightly elliptical micelle structure with a hydrophobic PBLG inner core and a hydrophilic PEG outer shell. The micelle structure was dynamically stable during the simulation, with the PEG blocks showing a compact helical conFormation and the PBLG blocks an α-Helix Form. Multiple hydrogen bonds with solvent water molecules stabilized the helical conFormation of the PEG blocks, leading to their hydration as shown by longer residence times of water molecules near the PEG ether oxygen at...
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All-atom molecular dynamics study of a spherical micelle composed of N-acetylated poly(ethylene glycol)-poly(gamma-benzyl L-glutamate) block copolymers: a potential carrier of drug delivery systems for cancer.
The journal of physical chemistry. B, 2009Co-Authors: Hiroshi Kuramochi, Yoshimichi Andoh, Noriyuki Yoshii, Susumu OkazakiAbstract:An all-atom molecular dynamics simulation of a spherical micelle composed of amphiphilic N-acetylated poly(ethylene glycol)-poly(gamma-benzyl L-glutamate) (PEG-PBLG-Ac) block copolymers was perFormed in aqueous solution at 298.15 K and 1 atm. Such copolymers have received considerable attention as carriers in drug delivery systems. In this study, we used copolymers consisting of 11 EG units and 9 BLG units as models. Starting from the copolymers arranged spherically, the calculation predicted an equilibrium state consisting of a slightly elliptical micelle structure with a hydrophobic PBLG inner core and a hydrophilic PEG outer shell. The micelle structure was dynamically stable during the simulation, with the PEG blocks showing a compact helical conFormation and the PBLG blocks an alpha-Helix Form. Multiple hydrogen bonds with solvent water molecules stabilized the helical conFormation of the PEG blocks, leading to their hydration as shown by longer residence times of water molecules near the PEG ether oxygen atoms compared with that of bulk water. Some water molecules have also been found distributed within the hydrophobic core; they showed continuous exchange with bulk water during the simulation. Those molecules existed mostly as a cluster in spaces between the copolymers, Forming hydrogen bonds among themselves as well as with the hydrophobic core through hydrophilic groups such as esters and amides. The water molecules Forming hydrogen bonds with the micelle may play an important role in the stabilization of the micelle structure.
Donghee Park - One of the best experts on this subject based on the ideXlab platform.
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Sound Packing DNA: packing open circular DNA with low-intensity ultrasound
Scientific Reports, 2015Co-Authors: Donghee Park, Bong-kwang Jung, Hyunjin Park, Hyungbeen Lee, Gyudo Lee, Jingam Park, Unchul Shin, Jong Ho Won, Jin Woo Chang, Sangwoo LeeAbstract:Supercoiling DNA (folding DNA into a more compact molecule) from open circular Forms requires significant bending energy. The double Helix is coiled into a higher order Helix Form; thus it occupies a smaller footprint. Compact packing of DNA is essential to improve the efficiency of gene delivery, which has broad implications in biology and pharmaceutical research. Here we show that low-intensity pulsed ultrasound can pack open circular DNA into supercoil Form. Plasmid DNA subjected to 5.4 mW/cm^2 intensity ultrasound showed significant (p-values
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sound packing dna packing open circular dna with low intensity ultrasound
Scientific Reports, 2015Co-Authors: Donghee Park, Bong-kwang Jung, Hyunjin Park, Hyungbeen Lee, Gyudo Lee, Jingam Park, Unchul Shin, Jong Ho Won, Jin Woo Chang, Sangwoo LeeAbstract:Supercoiling DNA (folding DNA into a more compact molecule) from open circular Forms requires significant bending energy. The double Helix is coiled into a higher order Helix Form; thus it occupies a smaller footprint. Compact packing of DNA is essential to improve the efficiency of gene delivery, which has broad implications in biology and pharmaceutical research. Here we show that low-intensity pulsed ultrasound can pack open circular DNA into supercoil Form. Plasmid DNA subjected to 5.4 mW/cm2 intensity ultrasound showed significant (p-values <0.001) supercoiling compared to DNA without exposure to ultrasound. Radiation force induced from ultrasound and dragging force from the fluid are believed to be the main factors that cause supercoiling. This study provides the first evidence to show that low-intensity ultrasound can directly alter DNA topology. We anticipate our results to be a starting point for improved non-viral gene delivery.
Hiroshi Kuramochi - One of the best experts on this subject based on the ideXlab platform.
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all atom molecular dynamics study of a spherical micelle composed of n acetylated poly ethylene glycol poly gamma benzyl l glutamate block copolymers a potential carrier of drug delivery systems for cancer
Journal of Physical Chemistry B, 2009Co-Authors: Hiroshi Kuramochi, Yoshimichi Andoh, Noriyuki Yoshii, Susumu OkazakiAbstract:An all-atom molecular dynamics simulation of a spherical micelle composed of amphiphilic N-acetylated poly(ethylene glycol)−poly(γ-benzyl l-glutamate) (PEG−PBLG-Ac) block copolymers was perFormed in aqueous solution at 298.15 K and 1 atm. Such copolymers have received considerable attention as carriers in drug delivery systems. In this study, we used copolymers consisting of 11 EG units and 9 BLG units as models. Starting from the copolymers arranged spherically, the calculation predicted an equilibrium state consisting of a slightly elliptical micelle structure with a hydrophobic PBLG inner core and a hydrophilic PEG outer shell. The micelle structure was dynamically stable during the simulation, with the PEG blocks showing a compact helical conFormation and the PBLG blocks an α-Helix Form. Multiple hydrogen bonds with solvent water molecules stabilized the helical conFormation of the PEG blocks, leading to their hydration as shown by longer residence times of water molecules near the PEG ether oxygen at...
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All-atom molecular dynamics study of a spherical micelle composed of N-acetylated poly(ethylene glycol)-poly(gamma-benzyl L-glutamate) block copolymers: a potential carrier of drug delivery systems for cancer.
The journal of physical chemistry. B, 2009Co-Authors: Hiroshi Kuramochi, Yoshimichi Andoh, Noriyuki Yoshii, Susumu OkazakiAbstract:An all-atom molecular dynamics simulation of a spherical micelle composed of amphiphilic N-acetylated poly(ethylene glycol)-poly(gamma-benzyl L-glutamate) (PEG-PBLG-Ac) block copolymers was perFormed in aqueous solution at 298.15 K and 1 atm. Such copolymers have received considerable attention as carriers in drug delivery systems. In this study, we used copolymers consisting of 11 EG units and 9 BLG units as models. Starting from the copolymers arranged spherically, the calculation predicted an equilibrium state consisting of a slightly elliptical micelle structure with a hydrophobic PBLG inner core and a hydrophilic PEG outer shell. The micelle structure was dynamically stable during the simulation, with the PEG blocks showing a compact helical conFormation and the PBLG blocks an alpha-Helix Form. Multiple hydrogen bonds with solvent water molecules stabilized the helical conFormation of the PEG blocks, leading to their hydration as shown by longer residence times of water molecules near the PEG ether oxygen atoms compared with that of bulk water. Some water molecules have also been found distributed within the hydrophobic core; they showed continuous exchange with bulk water during the simulation. Those molecules existed mostly as a cluster in spaces between the copolymers, Forming hydrogen bonds among themselves as well as with the hydrophobic core through hydrophilic groups such as esters and amides. The water molecules Forming hydrogen bonds with the micelle may play an important role in the stabilization of the micelle structure.
Gyudo Lee - One of the best experts on this subject based on the ideXlab platform.
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Sound Packing DNA: packing open circular DNA with low-intensity ultrasound
Scientific Reports, 2015Co-Authors: Donghee Park, Bong-kwang Jung, Hyunjin Park, Hyungbeen Lee, Gyudo Lee, Jingam Park, Unchul Shin, Jong Ho Won, Jin Woo Chang, Sangwoo LeeAbstract:Supercoiling DNA (folding DNA into a more compact molecule) from open circular Forms requires significant bending energy. The double Helix is coiled into a higher order Helix Form; thus it occupies a smaller footprint. Compact packing of DNA is essential to improve the efficiency of gene delivery, which has broad implications in biology and pharmaceutical research. Here we show that low-intensity pulsed ultrasound can pack open circular DNA into supercoil Form. Plasmid DNA subjected to 5.4 mW/cm^2 intensity ultrasound showed significant (p-values
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sound packing dna packing open circular dna with low intensity ultrasound
Scientific Reports, 2015Co-Authors: Donghee Park, Bong-kwang Jung, Hyunjin Park, Hyungbeen Lee, Gyudo Lee, Jingam Park, Unchul Shin, Jong Ho Won, Jin Woo Chang, Sangwoo LeeAbstract:Supercoiling DNA (folding DNA into a more compact molecule) from open circular Forms requires significant bending energy. The double Helix is coiled into a higher order Helix Form; thus it occupies a smaller footprint. Compact packing of DNA is essential to improve the efficiency of gene delivery, which has broad implications in biology and pharmaceutical research. Here we show that low-intensity pulsed ultrasound can pack open circular DNA into supercoil Form. Plasmid DNA subjected to 5.4 mW/cm2 intensity ultrasound showed significant (p-values <0.001) supercoiling compared to DNA without exposure to ultrasound. Radiation force induced from ultrasound and dragging force from the fluid are believed to be the main factors that cause supercoiling. This study provides the first evidence to show that low-intensity ultrasound can directly alter DNA topology. We anticipate our results to be a starting point for improved non-viral gene delivery.