The Experts below are selected from a list of 3525 Experts worldwide ranked by ideXlab platform
Kenneth E. Blick - One of the best experts on this subject based on the ideXlab platform.
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passive Hemagglutination Inhibition Test for diagnosis of brown recluse spider bite envenomation
Clinical Chemistry, 1993Co-Authors: Steven M. Barrett, M Rominejenkins, Kenneth E. BlickAbstract:Our goal was to recreate a passive Hemagglutination Inhibition (PHAI) Test to diagnose brown recluse spider (BRS; Loxosceles reclusa) bite envenomation for treatment trials. Guinea pigs received intradermal injections of concentrated spider venom from the following species: Loxosceles reclusa, Argiope aurantia, Argiope trifasciata, Phidippus audax, and Lycosa frondicola. Skin lesion exudate was collected and Tested with the BRS venom PHAI assay. From 51 separate collections of exudate, Test sensitivity was 90% as long as 3 days after venom injection. Specificity was 100% with venom from the other spider species listed above in vivo (7 Test samples) and in vitro (5 Test samples), as well as with random bacterial exudate with and without added serial dilutions of BRS venom (10 Test samples). The Test was reproducible over repetitive assays to within one 10-fold dilution. A positive PHAI Test result could function as an entry criterion for BRS bite victims in human treatment trials.
Wang Dong-li - One of the best experts on this subject based on the ideXlab platform.
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Improvement of Reverse Passive Hemagglutination and Hemagglutination Inhibition Test for Xinjiang Hemorrhagic Fever Virus
Endemic Disease Bulletin, 2004Co-Authors: Wang Dong-liAbstract:A method of reverse passive Hemagglutination and Hemagglutination Inhibition Test (RPHA and RPHI) for Xinjiang hemorrhagic fever virus has been developed by using glutaraldehyde fixed and tannic acid treated sheep red cells, sensitized with IgG which was abstracted from high titer (1∶1 024 in CFT) of immune sera of rabbits immunized with virus suspension through intra-lymphanodes injection. It is suitable for use in virus antigen and antibody detection for the samples of any animal origin. The freezing dried preparation conserved under 4℃ may sustain the activity over 1 year at least. The author recommends that the RPHA and RPHI can be used for clinical diagnosis and epidemiological survey of Xinjiang hemorrhagic fever virus infection.
Jerzy Romanowski - One of the best experts on this subject based on the ideXlab platform.
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antibodies to alphavirus flavivirus and bunyavirus arboviruses in house sparrows passer domesticus and tree sparrows p montanus in poland
Avian Diseases, 1998Co-Authors: Zina Juricova, J. Pinowski, Kyuhwang Hahm, Ivan Literak, Jerzy RomanowskiAbstract:SUMMARY. Sparrows from central Poland were examined by a Hemagglutination-Inhibition Test (titer > 20) for the presence of antibodies to arboviruses, between 1995 and 1996. In house sparrows (Passer domesticus) (n = 179), antibodies to Sindbis, West Nile, tick-borne encephalitis, Tahyna, and eialovo viruses were detected at seroprevalences of 1.1%, 2.8%, 1.1%, 2.8%, and 1.1%, respectively. In tree sparrows (P montanus) (n = 33), antibodies to the Sindbis, West Nile, and Tahynia viruses were detected at seroprevalences of 9.1%, 12.1%, and 3.0%, respectively.
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antibodies to alphavirus flavivirus and bunyavirus arboviruses in house sparrows passer domesticus and tree sparrows p montanus in poland
Avian Diseases, 1998Co-Authors: Zina Juricova, J. Pinowski, Kyuhwang Hahm, Ivan Literak, Jerzy RomanowskiAbstract:Sparrows from central Poland were examined by a Hemagglutination-Inhibition Test (titer > or = 20) for the presence of antibodies to arboviruses, between 1995 and 1996. In house sparrows (Passer domesticus) (n = 179), antibodies to Sindbis, West Nile, tick-borne encephalitis, Tahyna, and Calovo viruses were detected at seroprevalences of 1.1%, 2.8%, 1.1%, 2.8%, and 1.1%, respectively. In tree sparrows (P. montanus) (n = 33), antibodies to the Sindbis, West Nile, and Tahyna viruses were detected at seroprevalences of 9.1%, 12.1%, and 3.0%, respectively.
Steven M. Barrett - One of the best experts on this subject based on the ideXlab platform.
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passive Hemagglutination Inhibition Test for diagnosis of brown recluse spider bite envenomation
Clinical Chemistry, 1993Co-Authors: Steven M. Barrett, M Rominejenkins, Kenneth E. BlickAbstract:Our goal was to recreate a passive Hemagglutination Inhibition (PHAI) Test to diagnose brown recluse spider (BRS; Loxosceles reclusa) bite envenomation for treatment trials. Guinea pigs received intradermal injections of concentrated spider venom from the following species: Loxosceles reclusa, Argiope aurantia, Argiope trifasciata, Phidippus audax, and Lycosa frondicola. Skin lesion exudate was collected and Tested with the BRS venom PHAI assay. From 51 separate collections of exudate, Test sensitivity was 90% as long as 3 days after venom injection. Specificity was 100% with venom from the other spider species listed above in vivo (7 Test samples) and in vitro (5 Test samples), as well as with random bacterial exudate with and without added serial dilutions of BRS venom (10 Test samples). The Test was reproducible over repetitive assays to within one 10-fold dilution. A positive PHAI Test result could function as an entry criterion for BRS bite victims in human treatment trials.
Huanchun Chen - One of the best experts on this subject based on the ideXlab platform.
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latex agglutination Test for monitoring antibodies to avian influenza virus subtype h5n1
Journal of Clinical Microbiology, 2005Co-Authors: Xiaojuan Xu, Hongchao Li, Zhengjun Yu, Huanchun ChenAbstract:A latex agglutination Test (LAT) based on polystyrene beads sensitized with inactivated avian influenza virus H5N1 particles was developed. Compared with the Hemagglutination Inhibition Test, the sensitivity and specificity of the LAT were 88.8 and 97.6%, respectively, in detecting 830 serum samples from vaccinated chickens. The Test has application potential in field practice.