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Mark E Rupp - One of the best experts on this subject based on the ideXlab platform.
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characterization of the relationship between polysaccharide intercellular adhesin and Hemagglutination in staphylococcus epidermidis
The Journal of Infectious Diseases, 1999Co-Authors: Paul D Fey, Joseph S Ulphani, Friedrich Gotz, Christine Heilmann, Dietrich Mack, Mark E RuppAbstract:To determine whether a relationship exists between biofilm formation and Hemagglutination in Staphylococcus epidermidis, 20 skin isolates and 19 prosthetic valve endocarditis isolates were characterized for biofilm formation, Hemagglutination, and the presence of a 357-bp polymerase chain reaction product within icaA. A strong association existed between biofilm formation, which has been linked to strains that produce polysaccharide intercellular adhesin (PIA), and Hemagglutination. Strains that produced biofilm were significantly (P <.001) more likely to mediate Hemagglutination (16 biofilm-positive/hemaggiutination-positive strains and 19 biofilm-hegative/hemaggiutination-negative strains) within the 39 clinical strains tested. In addition, Staphylococcus carnosus TM300, a biofilm-negative, Hemagglutination-negative strain, carrying the ica operon-containing plasmid pCN27, produced significant biofilm on glass and mediated Hemagglutination (≥1/128). It was concluded that production of PIA and Hemagglutination are strongly associated and that PIA, at least in part, mediates Hemagglutination in S. epidermidis.
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Essential functional role of the polysaccharide intercellular adhesin of Staphylococcus epidermidis in Hemagglutination.
Infection and immunity, 1999Co-Authors: Dietrich Mack, Joachim Riedewald, Holger Rohde, Tim Magnus, Hubert Feucht, Holger A. Elsner, Rainer Laufs, Mark E RuppAbstract:Hemagglutination of erythrocytes is a common property of Staphylococcus epidermidis strains, which is related to adherence and biofilm formation and may be essential for the pathogenesis of biomaterial-associated infections caused by S. epidermidis. In three independent biofilm-producing, Hemagglutination-positive S. epidermidis isolates, interruption of the icaADBC operon essential for polysaccharide intercellular adhesin (PIA) synthesis by Tn917 insertions led to a Hemagglutination-negative phenotype. An immunoglobulin G fraction of antiserum to PIA greatly reduced Hemagglutination. Purified PIA led to a 64-fold decrease of Hemagglutination titers of these strains; however, it did not mediate Hemagglutination by itself. These observations define PIA as the hemagglutinin of S. epidermidis or at least as its major functional component.
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Characterization of the Relationship between Polysaccharide Intercellular Adhesin and Hemagglutination in Staphylococcus epidermidis
The Journal of infectious diseases, 1999Co-Authors: Paul D Fey, Joseph S Ulphani, Friedrich Gotz, Christine Heilmann, Dietrich Mack, Mark E RuppAbstract:To determine whether a relationship exists between biofilm formation and Hemagglutination in Staphylococcus epidermidis, 20 skin isolates and 19 prosthetic valve endocarditis isolates were characterized for biofilm formation, Hemagglutination, and the presence of a 357-bp polymerase chain reaction product within icaA. A strong association existed between biofilm formation, which has been linked to strains that produce polysaccharide intercellular adhesin (PIA), and Hemagglutination. Strains that produced biofilm were significantly (P
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Characterization of the Hemagglutinin of Staphylococcus epidermidis
The Journal of Infectious Diseases, 1995Co-Authors: Mark E Rupp, N. Sloot, H.-g. W. Meyer, Soren GatermannAbstract:: Adherence to biomaterials and production of biofilm is thought to be pivotal in the pathogenesis of prosthetic device infection by Staphylococcus epidermidis. In this study a strong association (P < .001) of Hemagglutination with adherence and biofilm production was observed. Hemagglutination was not associated with cell surface hydrophobicity (P = .906). Hemagglutination inhibition studies revealed that Hemagglutination was not affected by heat, pH, cation concentration, proteolytic enzymes, biologic detergent, serum proteins, or subinhibitory antibiotics. Hemagglutination was abolished by periodate oxidation and digestion with glycosidases. It was markedly inhibited by beta-lactose and its monosaccharide constituents in a concentration-dependent fashion. Hemagglutinin expression depended on the presence of glucose. Chemical analysis of a partially purified hemagglutinin preparation and cell-free hemagglutinating supernatants revealed little or no protein and small quantities of reducing sugars, pentose, ketose, hexosamine, uronic acid, and phosphate. Hemagglutinin of S. epidermidis appears to be a polysaccharide distinct from other known adhesins of S. epidermidis.
Dietrich Mack - One of the best experts on this subject based on the ideXlab platform.
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characterization of the relationship between polysaccharide intercellular adhesin and Hemagglutination in staphylococcus epidermidis
The Journal of Infectious Diseases, 1999Co-Authors: Paul D Fey, Joseph S Ulphani, Friedrich Gotz, Christine Heilmann, Dietrich Mack, Mark E RuppAbstract:To determine whether a relationship exists between biofilm formation and Hemagglutination in Staphylococcus epidermidis, 20 skin isolates and 19 prosthetic valve endocarditis isolates were characterized for biofilm formation, Hemagglutination, and the presence of a 357-bp polymerase chain reaction product within icaA. A strong association existed between biofilm formation, which has been linked to strains that produce polysaccharide intercellular adhesin (PIA), and Hemagglutination. Strains that produced biofilm were significantly (P <.001) more likely to mediate Hemagglutination (16 biofilm-positive/hemaggiutination-positive strains and 19 biofilm-hegative/hemaggiutination-negative strains) within the 39 clinical strains tested. In addition, Staphylococcus carnosus TM300, a biofilm-negative, Hemagglutination-negative strain, carrying the ica operon-containing plasmid pCN27, produced significant biofilm on glass and mediated Hemagglutination (≥1/128). It was concluded that production of PIA and Hemagglutination are strongly associated and that PIA, at least in part, mediates Hemagglutination in S. epidermidis.
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Essential functional role of the polysaccharide intercellular adhesin of Staphylococcus epidermidis in Hemagglutination.
Infection and immunity, 1999Co-Authors: Dietrich Mack, Joachim Riedewald, Holger Rohde, Tim Magnus, Hubert Feucht, Holger A. Elsner, Rainer Laufs, Mark E RuppAbstract:Hemagglutination of erythrocytes is a common property of Staphylococcus epidermidis strains, which is related to adherence and biofilm formation and may be essential for the pathogenesis of biomaterial-associated infections caused by S. epidermidis. In three independent biofilm-producing, Hemagglutination-positive S. epidermidis isolates, interruption of the icaADBC operon essential for polysaccharide intercellular adhesin (PIA) synthesis by Tn917 insertions led to a Hemagglutination-negative phenotype. An immunoglobulin G fraction of antiserum to PIA greatly reduced Hemagglutination. Purified PIA led to a 64-fold decrease of Hemagglutination titers of these strains; however, it did not mediate Hemagglutination by itself. These observations define PIA as the hemagglutinin of S. epidermidis or at least as its major functional component.
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Characterization of the Relationship between Polysaccharide Intercellular Adhesin and Hemagglutination in Staphylococcus epidermidis
The Journal of infectious diseases, 1999Co-Authors: Paul D Fey, Joseph S Ulphani, Friedrich Gotz, Christine Heilmann, Dietrich Mack, Mark E RuppAbstract:To determine whether a relationship exists between biofilm formation and Hemagglutination in Staphylococcus epidermidis, 20 skin isolates and 19 prosthetic valve endocarditis isolates were characterized for biofilm formation, Hemagglutination, and the presence of a 357-bp polymerase chain reaction product within icaA. A strong association existed between biofilm formation, which has been linked to strains that produce polysaccharide intercellular adhesin (PIA), and Hemagglutination. Strains that produced biofilm were significantly (P
Paul D Fey - One of the best experts on this subject based on the ideXlab platform.
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characterization of the relationship between polysaccharide intercellular adhesin and Hemagglutination in staphylococcus epidermidis
The Journal of Infectious Diseases, 1999Co-Authors: Paul D Fey, Joseph S Ulphani, Friedrich Gotz, Christine Heilmann, Dietrich Mack, Mark E RuppAbstract:To determine whether a relationship exists between biofilm formation and Hemagglutination in Staphylococcus epidermidis, 20 skin isolates and 19 prosthetic valve endocarditis isolates were characterized for biofilm formation, Hemagglutination, and the presence of a 357-bp polymerase chain reaction product within icaA. A strong association existed between biofilm formation, which has been linked to strains that produce polysaccharide intercellular adhesin (PIA), and Hemagglutination. Strains that produced biofilm were significantly (P <.001) more likely to mediate Hemagglutination (16 biofilm-positive/hemaggiutination-positive strains and 19 biofilm-hegative/hemaggiutination-negative strains) within the 39 clinical strains tested. In addition, Staphylococcus carnosus TM300, a biofilm-negative, Hemagglutination-negative strain, carrying the ica operon-containing plasmid pCN27, produced significant biofilm on glass and mediated Hemagglutination (≥1/128). It was concluded that production of PIA and Hemagglutination are strongly associated and that PIA, at least in part, mediates Hemagglutination in S. epidermidis.
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Characterization of the Relationship between Polysaccharide Intercellular Adhesin and Hemagglutination in Staphylococcus epidermidis
The Journal of infectious diseases, 1999Co-Authors: Paul D Fey, Joseph S Ulphani, Friedrich Gotz, Christine Heilmann, Dietrich Mack, Mark E RuppAbstract:To determine whether a relationship exists between biofilm formation and Hemagglutination in Staphylococcus epidermidis, 20 skin isolates and 19 prosthetic valve endocarditis isolates were characterized for biofilm formation, Hemagglutination, and the presence of a 357-bp polymerase chain reaction product within icaA. A strong association existed between biofilm formation, which has been linked to strains that produce polysaccharide intercellular adhesin (PIA), and Hemagglutination. Strains that produced biofilm were significantly (P
Friedrich Gotz - One of the best experts on this subject based on the ideXlab platform.
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characterization of the relationship between polysaccharide intercellular adhesin and Hemagglutination in staphylococcus epidermidis
The Journal of Infectious Diseases, 1999Co-Authors: Paul D Fey, Joseph S Ulphani, Friedrich Gotz, Christine Heilmann, Dietrich Mack, Mark E RuppAbstract:To determine whether a relationship exists between biofilm formation and Hemagglutination in Staphylococcus epidermidis, 20 skin isolates and 19 prosthetic valve endocarditis isolates were characterized for biofilm formation, Hemagglutination, and the presence of a 357-bp polymerase chain reaction product within icaA. A strong association existed between biofilm formation, which has been linked to strains that produce polysaccharide intercellular adhesin (PIA), and Hemagglutination. Strains that produced biofilm were significantly (P <.001) more likely to mediate Hemagglutination (16 biofilm-positive/hemaggiutination-positive strains and 19 biofilm-hegative/hemaggiutination-negative strains) within the 39 clinical strains tested. In addition, Staphylococcus carnosus TM300, a biofilm-negative, Hemagglutination-negative strain, carrying the ica operon-containing plasmid pCN27, produced significant biofilm on glass and mediated Hemagglutination (≥1/128). It was concluded that production of PIA and Hemagglutination are strongly associated and that PIA, at least in part, mediates Hemagglutination in S. epidermidis.
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Characterization of the Relationship between Polysaccharide Intercellular Adhesin and Hemagglutination in Staphylococcus epidermidis
The Journal of infectious diseases, 1999Co-Authors: Paul D Fey, Joseph S Ulphani, Friedrich Gotz, Christine Heilmann, Dietrich Mack, Mark E RuppAbstract:To determine whether a relationship exists between biofilm formation and Hemagglutination in Staphylococcus epidermidis, 20 skin isolates and 19 prosthetic valve endocarditis isolates were characterized for biofilm formation, Hemagglutination, and the presence of a 357-bp polymerase chain reaction product within icaA. A strong association existed between biofilm formation, which has been linked to strains that produce polysaccharide intercellular adhesin (PIA), and Hemagglutination. Strains that produced biofilm were significantly (P
Joseph S Ulphani - One of the best experts on this subject based on the ideXlab platform.
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characterization of the relationship between polysaccharide intercellular adhesin and Hemagglutination in staphylococcus epidermidis
The Journal of Infectious Diseases, 1999Co-Authors: Paul D Fey, Joseph S Ulphani, Friedrich Gotz, Christine Heilmann, Dietrich Mack, Mark E RuppAbstract:To determine whether a relationship exists between biofilm formation and Hemagglutination in Staphylococcus epidermidis, 20 skin isolates and 19 prosthetic valve endocarditis isolates were characterized for biofilm formation, Hemagglutination, and the presence of a 357-bp polymerase chain reaction product within icaA. A strong association existed between biofilm formation, which has been linked to strains that produce polysaccharide intercellular adhesin (PIA), and Hemagglutination. Strains that produced biofilm were significantly (P <.001) more likely to mediate Hemagglutination (16 biofilm-positive/hemaggiutination-positive strains and 19 biofilm-hegative/hemaggiutination-negative strains) within the 39 clinical strains tested. In addition, Staphylococcus carnosus TM300, a biofilm-negative, Hemagglutination-negative strain, carrying the ica operon-containing plasmid pCN27, produced significant biofilm on glass and mediated Hemagglutination (≥1/128). It was concluded that production of PIA and Hemagglutination are strongly associated and that PIA, at least in part, mediates Hemagglutination in S. epidermidis.
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Characterization of the Relationship between Polysaccharide Intercellular Adhesin and Hemagglutination in Staphylococcus epidermidis
The Journal of infectious diseases, 1999Co-Authors: Paul D Fey, Joseph S Ulphani, Friedrich Gotz, Christine Heilmann, Dietrich Mack, Mark E RuppAbstract:To determine whether a relationship exists between biofilm formation and Hemagglutination in Staphylococcus epidermidis, 20 skin isolates and 19 prosthetic valve endocarditis isolates were characterized for biofilm formation, Hemagglutination, and the presence of a 357-bp polymerase chain reaction product within icaA. A strong association existed between biofilm formation, which has been linked to strains that produce polysaccharide intercellular adhesin (PIA), and Hemagglutination. Strains that produced biofilm were significantly (P
