The Experts below are selected from a list of 567 Experts worldwide ranked by ideXlab platform

Albert Wingnang Leung - One of the best experts on this subject based on the ideXlab platform.

  • cellular uptake subcellular localization and photodamaging effect of temoporfin mthpc in nasopharyngeal carcinoma cells comparison with Hematoporphyrin Derivative
    Cancer Letters, 2000
    Co-Authors: Jie Chen, N H Cheung, Albert Wingnang Leung
    Abstract:

    Abstract Temoporfin (meta-tetra (hydroxyphenyl)chlorin; mTHPC) potentiated a 100-fold higher cytotoxic effect than Hematoporphyrin Derivative (HPD) on two nasopharyngeal carcinoma cell lines (HK1 and CNE2) in terms of the overall photodynamic therapy (PDT) dose. The cellular uptake, evaluated by flow cytometry and spectrophotometry demonstrated that mTHPC exhibited higher uptake ability than HPD. Confocal laser scanning microscopy detection for both the sensitizer and mitochondria probe on the same cell images revealed that both drugs accumulated diffusely in the cytoplasm and that mitochrondria is a target organelle. Photo-activation ruptured the mitochrondria, with more pronounced mitochondrial damage being observed in mTHPC-PDT course. This correlated well with the cell photokilling efficiency of mTHPC.

  • cellular uptake subcellular localization and photodamaging effect of temoporfin mthpc in nasopharyngeal carcinoma cells comparison with Hematoporphyrin Derivative
    Cancer Letters, 2000
    Co-Authors: Christine M N Yow, N H Cheung, Jiyao Chen, Nai Ki Mak, Albert Wingnang Leung
    Abstract:

    Temoporfin (meta-tetra (hydroxyphenyl)chlorin; mTHPC) potentiated a 100-fold higher cytotoxic effect than Hematoporphyrin Derivative (HPD) on two nasopharyngeal carcinoma cell lines (HK1 and CNE2) in terms of the overall photodynamic therapy (PDT) dose. The cellular uptake, evaluated by flow cytometry and spectrophotometry demonstrated that mTHPC exhibited higher uptake ability than HPD. Confocal laser scanning microscopy detection for both the sensitizer and mitochondria probe on the same cell images revealed that both drugs accumulated diffusely in the cytoplasm and that mitochrondria is a target organelle. Photo-activation ruptured the mitochrondria, with more pronounced mitochondrial damage being observed in mTHPC-PDT course. This correlated well with the cell photokilling efficiency of mTHPC.

N H Cheung - One of the best experts on this subject based on the ideXlab platform.

  • cellular uptake subcellular localization and photodamaging effect of temoporfin mthpc in nasopharyngeal carcinoma cells comparison with Hematoporphyrin Derivative
    Cancer Letters, 2000
    Co-Authors: Jie Chen, N H Cheung, Albert Wingnang Leung
    Abstract:

    Abstract Temoporfin (meta-tetra (hydroxyphenyl)chlorin; mTHPC) potentiated a 100-fold higher cytotoxic effect than Hematoporphyrin Derivative (HPD) on two nasopharyngeal carcinoma cell lines (HK1 and CNE2) in terms of the overall photodynamic therapy (PDT) dose. The cellular uptake, evaluated by flow cytometry and spectrophotometry demonstrated that mTHPC exhibited higher uptake ability than HPD. Confocal laser scanning microscopy detection for both the sensitizer and mitochondria probe on the same cell images revealed that both drugs accumulated diffusely in the cytoplasm and that mitochrondria is a target organelle. Photo-activation ruptured the mitochrondria, with more pronounced mitochondrial damage being observed in mTHPC-PDT course. This correlated well with the cell photokilling efficiency of mTHPC.

  • cellular uptake subcellular localization and photodamaging effect of temoporfin mthpc in nasopharyngeal carcinoma cells comparison with Hematoporphyrin Derivative
    Cancer Letters, 2000
    Co-Authors: Christine M N Yow, N H Cheung, Jiyao Chen, Nai Ki Mak, Albert Wingnang Leung
    Abstract:

    Temoporfin (meta-tetra (hydroxyphenyl)chlorin; mTHPC) potentiated a 100-fold higher cytotoxic effect than Hematoporphyrin Derivative (HPD) on two nasopharyngeal carcinoma cell lines (HK1 and CNE2) in terms of the overall photodynamic therapy (PDT) dose. The cellular uptake, evaluated by flow cytometry and spectrophotometry demonstrated that mTHPC exhibited higher uptake ability than HPD. Confocal laser scanning microscopy detection for both the sensitizer and mitochondria probe on the same cell images revealed that both drugs accumulated diffusely in the cytoplasm and that mitochrondria is a target organelle. Photo-activation ruptured the mitochrondria, with more pronounced mitochondrial damage being observed in mTHPC-PDT course. This correlated well with the cell photokilling efficiency of mTHPC.

Gianluca Valentini - One of the best experts on this subject based on the ideXlab platform.

  • fluorescence lifetime imaging of experimental tumors in Hematoporphyrin Derivative sensitized mice
    Photochemistry and Photobiology, 1997
    Co-Authors: Rinaldo Cubeddu, G Canti, Antonio Pifferi, Paola Taroni, Gianluca Valentini
    Abstract:

    : Tumor detection has been carried out in mice sensitized with Hematoporphyrin Derivative (HpD) by measuring the spatial distribution of the fluorescence lifetime of the exogenous compound. This result has been achieved using a time-gated video camera and a suitable mathematical processing that led to the so-called "lifetime images." Extensive experimental tests have been performed on mice bearing the MS-2 fibrosarcoma or the L1210 leukemia. Lifetime images of mice show that the fluorescence decay of HpD is appreciably slower in the tumor than in healthy tissues nearby, allowing a reliable detection of the neoplasia. The lengthening of the lifetime in tumors depends little on the drug dose, which in our experiments could be lowered down to 0.1 mg/kg body weight, still allowing a definite tumor detection. In order to ascertain the results achieved with the imaging apparatus, high-resolution spectroscopy, based on a time-correlated single photon counting system, has also been performed to measure the fluorescence lifetime of the drug inside the tumor and outside. The outcomes obtained with two techniques are in good agreement.

  • absorption spectrum of Hematoporphyrin Derivative in vivo in a murine tumor model
    Photochemistry and Photobiology, 1994
    Co-Authors: Rinaldo Cubeddu, G Canti, Mario Musolino, Antonio Pifferi, Paola Taroni, Gianluca Valentini
    Abstract:

    Time-resolved reflectance was used to measure the absorption spectrum of Hematoporphyrin Derivative (HpD) in vivo in a murine tumor model. Reflectance measurements were performed in the 600-640 nm range on mice bearing the L1210 leukemia. Then the animals were administered 25 mg/kg body weight of HpD intraperitoneally. One hour later the reflectance measurements were repeated. Fitting of the data using the diffusion theory allowed assessment of the absorption coefficient before and after the administration. As a difference between the latter and the former data, the in vivo absorption spectrum of HpD was evaluated. Maximum absorption was measured at 620-625 nm. Similar spectral behavior was obtained for HpD in solution in the presence of low-density lipoproteins.

Christine M N Yow - One of the best experts on this subject based on the ideXlab platform.

  • cellular uptake subcellular localization and photodamaging effect of temoporfin mthpc in nasopharyngeal carcinoma cells comparison with Hematoporphyrin Derivative
    Cancer Letters, 2000
    Co-Authors: Christine M N Yow, N H Cheung, Jiyao Chen, Nai Ki Mak, Albert Wingnang Leung
    Abstract:

    Temoporfin (meta-tetra (hydroxyphenyl)chlorin; mTHPC) potentiated a 100-fold higher cytotoxic effect than Hematoporphyrin Derivative (HPD) on two nasopharyngeal carcinoma cell lines (HK1 and CNE2) in terms of the overall photodynamic therapy (PDT) dose. The cellular uptake, evaluated by flow cytometry and spectrophotometry demonstrated that mTHPC exhibited higher uptake ability than HPD. Confocal laser scanning microscopy detection for both the sensitizer and mitochondria probe on the same cell images revealed that both drugs accumulated diffusely in the cytoplasm and that mitochrondria is a target organelle. Photo-activation ruptured the mitochrondria, with more pronounced mitochondrial damage being observed in mTHPC-PDT course. This correlated well with the cell photokilling efficiency of mTHPC.

Jie Chen - One of the best experts on this subject based on the ideXlab platform.

  • cellular uptake subcellular localization and photodamaging effect of temoporfin mthpc in nasopharyngeal carcinoma cells comparison with Hematoporphyrin Derivative
    Cancer Letters, 2000
    Co-Authors: Jie Chen, N H Cheung, Albert Wingnang Leung
    Abstract:

    Abstract Temoporfin (meta-tetra (hydroxyphenyl)chlorin; mTHPC) potentiated a 100-fold higher cytotoxic effect than Hematoporphyrin Derivative (HPD) on two nasopharyngeal carcinoma cell lines (HK1 and CNE2) in terms of the overall photodynamic therapy (PDT) dose. The cellular uptake, evaluated by flow cytometry and spectrophotometry demonstrated that mTHPC exhibited higher uptake ability than HPD. Confocal laser scanning microscopy detection for both the sensitizer and mitochondria probe on the same cell images revealed that both drugs accumulated diffusely in the cytoplasm and that mitochrondria is a target organelle. Photo-activation ruptured the mitochrondria, with more pronounced mitochondrial damage being observed in mTHPC-PDT course. This correlated well with the cell photokilling efficiency of mTHPC.