The Experts below are selected from a list of 56148 Experts worldwide ranked by ideXlab platform

Maria Kritsepikonstantinou - One of the best experts on this subject based on the ideXlab platform.

  • capillary Hemoglobin electrophoresis of healthy and anemic dogs quantification validation and reference intervals of Hemoglobin fractions
    PLOS ONE, 2019
    Co-Authors: Ioannis L Oikonomidis, Theodora K Tsouloufi, Mathios E Mylonakis, Maria Kritsepikonstantinou
    Abstract:

    : Despite the advances in canine medicine and the rapid gaining of attention of canine models in biomedical field and particularly in Hemoglobin genes research, the studies on canine Hemoglobin composition are sparse with ambiguous findings. Our aim was: i) to investigate the electrophoretic pattern of canine Hemoglobin and the possible effects of age, sex, and anemia using a capillary electrophoresis assay, and ii) to validate this assay and calculate reference intervals (RIs) for canine Hemoglobin fractions. Blood samples were collected from 53 healthy and 42 dogs with regenerative and non-regenerative anemias. The Sebia Capillarys 2 flex-piercing was used for Hemoglobin Analysis and it was validated using canine blood samples. R statistical language was employed for the statistical analyses. A major Hemoglobin fraction (named HbA0) and a minor one (named HbA2) were identified in 100% and 47.4% of samples, respectively. The within-run and between-run CV was 0.1% for HbA0, and 9.1% and 11.2% for HbA2, respectively. The extremely narrow range of HbA0 and HbA2 values hampered a linearity study using canine blood samples. The RIs for HbA0 and HbA2 were 98.9-100% and 0-1.1%, respectively. HbA0 and HbA2 values were not significantly correlated with age (P = 0.866) or reticulocyte count (P = 0.731). No differences were observed in the median HbA0 and HbA2 between the two sexes (P = 0.887), and healthy and anemic dogs (P = 0.805). In conclusion, the capillary electrophoresis revealed a major Hemoglobin fraction and an inconsistently present minor fraction. No effect of age, sex, anemia, or regenerative status of anemia was detected. The assay used was validated and RIs were generated, so as to be suitable for use in future investigations.

  • capillary Hemoglobin electrophoresis of healthy and anemic dogs quantification validation and reference intervals of Hemoglobin fractions
    bioRxiv, 2019
    Co-Authors: Ioannis L Oikonomidis, Theodora K Tsouloufi, Mathios E Mylonakis, Maria Kritsepikonstantinou
    Abstract:

    Abstract Despite the advances in canine medicine and the rapid gaining of attention of canine models in biomedical field and particularly in Hemoglobin genes research, the studies on canine Hemoglobin composition are sparse with ambiguous findings. Our aim was: i) to investigate the electrophoretic pattern of canine Hemoglobin and the possible effect of age, sex, and anemia using a capillary electrophoresis assay, and ii) to validate this assay and calculate reference intervals (RIs) for canine Hemoglobin fractions. Blood samples were collected from 53 healthy and 42 dogs with regenerative and non-regenerative anemias. The Sebia Capillarys 2 flex-piercing was used for Hemoglobin Analysis and it was validated using canine blood samples. R statistical language was employed for the statistical analyses. A major Hemoglobin fraction (named HbA0) and a minor one (named HbA2) were identified in 100% and 47.4% of samples, respectively. The within-run and between-run CV was 0.1% for HbA0 and 9.1% and 11.2% for HbA2, respectively. The extremely narrow range of HbA0 and HbA2 values hampered a linearity study using canine blood samples. The RIs for HbA0 and HbA2 were 98.9-100% and 0-1.1%, respectively. HbA0 and HbA2 values were not correlated with age (P=0.866). No differences were observed in the median HbA0 and HbA2 between the two sexes (P=0.823), and healthy and anemic dogs (P=0.805). In conclusion, the capillary electrophoresis revealed a major Hemoglobin fraction and an inconsistently present minor fraction. No effect of age, sex, or anemia was detected. The assay used was validated and RIs were generated, so as to be suitable for use in future investigations.

Melissa S Creary - One of the best experts on this subject based on the ideXlab platform.

  • paper based microchip electrophoresis for point of care Hemoglobin testing
    Analyst, 2020
    Co-Authors: Muhammad Noman Hasan, Arwa Fraiwan, Yunus Alapan, Asya Akkus, Julia Z Xu, Amy J Rezac, Nicholas Kocmich, Ran An, Melissa S Creary
    Abstract:

    Nearly 7% of the world's population live with a Hemoglobin variant. Hemoglobins S, C, and E are the most common and significant Hemoglobin variants worldwide. Sickle cell disease, caused by Hemoglobin S, is highly prevalent in sub-Saharan Africa and in tribal populations of Central India. Hemoglobin C is common in West Africa, and Hemoglobin E is common in Southeast Asia. Screening for significant Hemoglobin disorders is not currently feasible in many low-income countries with the high disease burden. Lack of early diagnosis leads to preventable high morbidity and mortality in children born with Hemoglobin variants in low-resource settings. Here, we describe HemeChip, the first miniaturized, paper-based, microchip electrophoresis platform for identifying the most common Hemoglobin variants easily and affordably at the point-of-care in low-resource settings. HemeChip test works with a drop of blood. HemeChip system guides the user step-by-step through the test procedure with animated on-screen instructions. Hemoglobin identification and quantification is automatically performed, and Hemoglobin types and percentages are displayed in an easily understandable, objective way. We show the feasibility and high accuracy of HemeChip via testing 768 subjects by clinical sites in the United States, Central India, sub-Saharan Africa, and Southeast Asia. Validation studies include Hemoglobin E testing in Bangkok, Thailand, and Hemoglobin S testing in Chhattisgarh, India, and in Kano, Nigeria, where the sickle cell disease burden is the highest in the world. Tests were performed by local users, including healthcare workers and clinical laboratory personnel. Study design, methods, and results are presented according to the Standards for Reporting Diagnostic Accuracy (STARD). HemeChip correctly identified all subjects with Hemoglobin S, C, and E variants with 100% sensitivity, and displayed an overall diagnostic accuracy of 98.4% in comparison to reference standard methods. HemeChip is a versatile, mass-producible microchip electrophoresis platform that addresses a major unmet need of decentralized Hemoglobin Analysis in resource-limited settings.

Yunus Alapan - One of the best experts on this subject based on the ideXlab platform.

  • paper based microchip electrophoresis for point of care Hemoglobin testing
    Analyst, 2020
    Co-Authors: Muhammad Noman Hasan, Arwa Fraiwan, Yunus Alapan, Asya Akkus, Julia Z Xu, Amy J Rezac, Nicholas Kocmich, Ran An, Melissa S Creary
    Abstract:

    Nearly 7% of the world's population live with a Hemoglobin variant. Hemoglobins S, C, and E are the most common and significant Hemoglobin variants worldwide. Sickle cell disease, caused by Hemoglobin S, is highly prevalent in sub-Saharan Africa and in tribal populations of Central India. Hemoglobin C is common in West Africa, and Hemoglobin E is common in Southeast Asia. Screening for significant Hemoglobin disorders is not currently feasible in many low-income countries with the high disease burden. Lack of early diagnosis leads to preventable high morbidity and mortality in children born with Hemoglobin variants in low-resource settings. Here, we describe HemeChip, the first miniaturized, paper-based, microchip electrophoresis platform for identifying the most common Hemoglobin variants easily and affordably at the point-of-care in low-resource settings. HemeChip test works with a drop of blood. HemeChip system guides the user step-by-step through the test procedure with animated on-screen instructions. Hemoglobin identification and quantification is automatically performed, and Hemoglobin types and percentages are displayed in an easily understandable, objective way. We show the feasibility and high accuracy of HemeChip via testing 768 subjects by clinical sites in the United States, Central India, sub-Saharan Africa, and Southeast Asia. Validation studies include Hemoglobin E testing in Bangkok, Thailand, and Hemoglobin S testing in Chhattisgarh, India, and in Kano, Nigeria, where the sickle cell disease burden is the highest in the world. Tests were performed by local users, including healthcare workers and clinical laboratory personnel. Study design, methods, and results are presented according to the Standards for Reporting Diagnostic Accuracy (STARD). HemeChip correctly identified all subjects with Hemoglobin S, C, and E variants with 100% sensitivity, and displayed an overall diagnostic accuracy of 98.4% in comparison to reference standard methods. HemeChip is a versatile, mass-producible microchip electrophoresis platform that addresses a major unmet need of decentralized Hemoglobin Analysis in resource-limited settings.

Ioannis L Oikonomidis - One of the best experts on this subject based on the ideXlab platform.

  • capillary Hemoglobin electrophoresis of healthy and anemic dogs quantification validation and reference intervals of Hemoglobin fractions
    PLOS ONE, 2019
    Co-Authors: Ioannis L Oikonomidis, Theodora K Tsouloufi, Mathios E Mylonakis, Maria Kritsepikonstantinou
    Abstract:

    : Despite the advances in canine medicine and the rapid gaining of attention of canine models in biomedical field and particularly in Hemoglobin genes research, the studies on canine Hemoglobin composition are sparse with ambiguous findings. Our aim was: i) to investigate the electrophoretic pattern of canine Hemoglobin and the possible effects of age, sex, and anemia using a capillary electrophoresis assay, and ii) to validate this assay and calculate reference intervals (RIs) for canine Hemoglobin fractions. Blood samples were collected from 53 healthy and 42 dogs with regenerative and non-regenerative anemias. The Sebia Capillarys 2 flex-piercing was used for Hemoglobin Analysis and it was validated using canine blood samples. R statistical language was employed for the statistical analyses. A major Hemoglobin fraction (named HbA0) and a minor one (named HbA2) were identified in 100% and 47.4% of samples, respectively. The within-run and between-run CV was 0.1% for HbA0, and 9.1% and 11.2% for HbA2, respectively. The extremely narrow range of HbA0 and HbA2 values hampered a linearity study using canine blood samples. The RIs for HbA0 and HbA2 were 98.9-100% and 0-1.1%, respectively. HbA0 and HbA2 values were not significantly correlated with age (P = 0.866) or reticulocyte count (P = 0.731). No differences were observed in the median HbA0 and HbA2 between the two sexes (P = 0.887), and healthy and anemic dogs (P = 0.805). In conclusion, the capillary electrophoresis revealed a major Hemoglobin fraction and an inconsistently present minor fraction. No effect of age, sex, anemia, or regenerative status of anemia was detected. The assay used was validated and RIs were generated, so as to be suitable for use in future investigations.

  • capillary Hemoglobin electrophoresis of healthy and anemic dogs quantification validation and reference intervals of Hemoglobin fractions
    bioRxiv, 2019
    Co-Authors: Ioannis L Oikonomidis, Theodora K Tsouloufi, Mathios E Mylonakis, Maria Kritsepikonstantinou
    Abstract:

    Abstract Despite the advances in canine medicine and the rapid gaining of attention of canine models in biomedical field and particularly in Hemoglobin genes research, the studies on canine Hemoglobin composition are sparse with ambiguous findings. Our aim was: i) to investigate the electrophoretic pattern of canine Hemoglobin and the possible effect of age, sex, and anemia using a capillary electrophoresis assay, and ii) to validate this assay and calculate reference intervals (RIs) for canine Hemoglobin fractions. Blood samples were collected from 53 healthy and 42 dogs with regenerative and non-regenerative anemias. The Sebia Capillarys 2 flex-piercing was used for Hemoglobin Analysis and it was validated using canine blood samples. R statistical language was employed for the statistical analyses. A major Hemoglobin fraction (named HbA0) and a minor one (named HbA2) were identified in 100% and 47.4% of samples, respectively. The within-run and between-run CV was 0.1% for HbA0 and 9.1% and 11.2% for HbA2, respectively. The extremely narrow range of HbA0 and HbA2 values hampered a linearity study using canine blood samples. The RIs for HbA0 and HbA2 were 98.9-100% and 0-1.1%, respectively. HbA0 and HbA2 values were not correlated with age (P=0.866). No differences were observed in the median HbA0 and HbA2 between the two sexes (P=0.823), and healthy and anemic dogs (P=0.805). In conclusion, the capillary electrophoresis revealed a major Hemoglobin fraction and an inconsistently present minor fraction. No effect of age, sex, or anemia was detected. The assay used was validated and RIs were generated, so as to be suitable for use in future investigations.

Muhammad Noman Hasan - One of the best experts on this subject based on the ideXlab platform.

  • paper based microchip electrophoresis for point of care Hemoglobin testing
    Analyst, 2020
    Co-Authors: Muhammad Noman Hasan, Arwa Fraiwan, Yunus Alapan, Asya Akkus, Julia Z Xu, Amy J Rezac, Nicholas Kocmich, Ran An, Melissa S Creary
    Abstract:

    Nearly 7% of the world's population live with a Hemoglobin variant. Hemoglobins S, C, and E are the most common and significant Hemoglobin variants worldwide. Sickle cell disease, caused by Hemoglobin S, is highly prevalent in sub-Saharan Africa and in tribal populations of Central India. Hemoglobin C is common in West Africa, and Hemoglobin E is common in Southeast Asia. Screening for significant Hemoglobin disorders is not currently feasible in many low-income countries with the high disease burden. Lack of early diagnosis leads to preventable high morbidity and mortality in children born with Hemoglobin variants in low-resource settings. Here, we describe HemeChip, the first miniaturized, paper-based, microchip electrophoresis platform for identifying the most common Hemoglobin variants easily and affordably at the point-of-care in low-resource settings. HemeChip test works with a drop of blood. HemeChip system guides the user step-by-step through the test procedure with animated on-screen instructions. Hemoglobin identification and quantification is automatically performed, and Hemoglobin types and percentages are displayed in an easily understandable, objective way. We show the feasibility and high accuracy of HemeChip via testing 768 subjects by clinical sites in the United States, Central India, sub-Saharan Africa, and Southeast Asia. Validation studies include Hemoglobin E testing in Bangkok, Thailand, and Hemoglobin S testing in Chhattisgarh, India, and in Kano, Nigeria, where the sickle cell disease burden is the highest in the world. Tests were performed by local users, including healthcare workers and clinical laboratory personnel. Study design, methods, and results are presented according to the Standards for Reporting Diagnostic Accuracy (STARD). HemeChip correctly identified all subjects with Hemoglobin S, C, and E variants with 100% sensitivity, and displayed an overall diagnostic accuracy of 98.4% in comparison to reference standard methods. HemeChip is a versatile, mass-producible microchip electrophoresis platform that addresses a major unmet need of decentralized Hemoglobin Analysis in resource-limited settings.