The Experts below are selected from a list of 234 Experts worldwide ranked by ideXlab platform

Maria Kritsepikonstantinou - One of the best experts on this subject based on the ideXlab platform.

  • capillary Hemoglobin Electrophoresis of healthy and anemic dogs quantification validation and reference intervals of Hemoglobin fractions
    PLOS ONE, 2019
    Co-Authors: Ioannis L Oikonomidis, Theodora K Tsouloufi, Mathios E Mylonakis, Maria Kritsepikonstantinou
    Abstract:

    : Despite the advances in canine medicine and the rapid gaining of attention of canine models in biomedical field and particularly in Hemoglobin genes research, the studies on canine Hemoglobin composition are sparse with ambiguous findings. Our aim was: i) to investigate the electrophoretic pattern of canine Hemoglobin and the possible effects of age, sex, and anemia using a capillary Electrophoresis assay, and ii) to validate this assay and calculate reference intervals (RIs) for canine Hemoglobin fractions. Blood samples were collected from 53 healthy and 42 dogs with regenerative and non-regenerative anemias. The Sebia Capillarys 2 flex-piercing was used for Hemoglobin analysis and it was validated using canine blood samples. R statistical language was employed for the statistical analyses. A major Hemoglobin fraction (named HbA0) and a minor one (named HbA2) were identified in 100% and 47.4% of samples, respectively. The within-run and between-run CV was 0.1% for HbA0, and 9.1% and 11.2% for HbA2, respectively. The extremely narrow range of HbA0 and HbA2 values hampered a linearity study using canine blood samples. The RIs for HbA0 and HbA2 were 98.9-100% and 0-1.1%, respectively. HbA0 and HbA2 values were not significantly correlated with age (P = 0.866) or reticulocyte count (P = 0.731). No differences were observed in the median HbA0 and HbA2 between the two sexes (P = 0.887), and healthy and anemic dogs (P = 0.805). In conclusion, the capillary Electrophoresis revealed a major Hemoglobin fraction and an inconsistently present minor fraction. No effect of age, sex, anemia, or regenerative status of anemia was detected. The assay used was validated and RIs were generated, so as to be suitable for use in future investigations.

  • capillary Hemoglobin Electrophoresis of healthy and anemic dogs quantification validation and reference intervals of Hemoglobin fractions
    bioRxiv, 2019
    Co-Authors: Ioannis L Oikonomidis, Theodora K Tsouloufi, Mathios E Mylonakis, Maria Kritsepikonstantinou
    Abstract:

    Abstract Despite the advances in canine medicine and the rapid gaining of attention of canine models in biomedical field and particularly in Hemoglobin genes research, the studies on canine Hemoglobin composition are sparse with ambiguous findings. Our aim was: i) to investigate the electrophoretic pattern of canine Hemoglobin and the possible effect of age, sex, and anemia using a capillary Electrophoresis assay, and ii) to validate this assay and calculate reference intervals (RIs) for canine Hemoglobin fractions. Blood samples were collected from 53 healthy and 42 dogs with regenerative and non-regenerative anemias. The Sebia Capillarys 2 flex-piercing was used for Hemoglobin analysis and it was validated using canine blood samples. R statistical language was employed for the statistical analyses. A major Hemoglobin fraction (named HbA0) and a minor one (named HbA2) were identified in 100% and 47.4% of samples, respectively. The within-run and between-run CV was 0.1% for HbA0 and 9.1% and 11.2% for HbA2, respectively. The extremely narrow range of HbA0 and HbA2 values hampered a linearity study using canine blood samples. The RIs for HbA0 and HbA2 were 98.9-100% and 0-1.1%, respectively. HbA0 and HbA2 values were not correlated with age (P=0.866). No differences were observed in the median HbA0 and HbA2 between the two sexes (P=0.823), and healthy and anemic dogs (P=0.805). In conclusion, the capillary Electrophoresis revealed a major Hemoglobin fraction and an inconsistently present minor fraction. No effect of age, sex, or anemia was detected. The assay used was validated and RIs were generated, so as to be suitable for use in future investigations.

Ioannis L Oikonomidis - One of the best experts on this subject based on the ideXlab platform.

  • capillary Hemoglobin Electrophoresis of healthy and anemic dogs quantification validation and reference intervals of Hemoglobin fractions
    PLOS ONE, 2019
    Co-Authors: Ioannis L Oikonomidis, Theodora K Tsouloufi, Mathios E Mylonakis, Maria Kritsepikonstantinou
    Abstract:

    : Despite the advances in canine medicine and the rapid gaining of attention of canine models in biomedical field and particularly in Hemoglobin genes research, the studies on canine Hemoglobin composition are sparse with ambiguous findings. Our aim was: i) to investigate the electrophoretic pattern of canine Hemoglobin and the possible effects of age, sex, and anemia using a capillary Electrophoresis assay, and ii) to validate this assay and calculate reference intervals (RIs) for canine Hemoglobin fractions. Blood samples were collected from 53 healthy and 42 dogs with regenerative and non-regenerative anemias. The Sebia Capillarys 2 flex-piercing was used for Hemoglobin analysis and it was validated using canine blood samples. R statistical language was employed for the statistical analyses. A major Hemoglobin fraction (named HbA0) and a minor one (named HbA2) were identified in 100% and 47.4% of samples, respectively. The within-run and between-run CV was 0.1% for HbA0, and 9.1% and 11.2% for HbA2, respectively. The extremely narrow range of HbA0 and HbA2 values hampered a linearity study using canine blood samples. The RIs for HbA0 and HbA2 were 98.9-100% and 0-1.1%, respectively. HbA0 and HbA2 values were not significantly correlated with age (P = 0.866) or reticulocyte count (P = 0.731). No differences were observed in the median HbA0 and HbA2 between the two sexes (P = 0.887), and healthy and anemic dogs (P = 0.805). In conclusion, the capillary Electrophoresis revealed a major Hemoglobin fraction and an inconsistently present minor fraction. No effect of age, sex, anemia, or regenerative status of anemia was detected. The assay used was validated and RIs were generated, so as to be suitable for use in future investigations.

  • capillary Hemoglobin Electrophoresis of healthy and anemic dogs quantification validation and reference intervals of Hemoglobin fractions
    bioRxiv, 2019
    Co-Authors: Ioannis L Oikonomidis, Theodora K Tsouloufi, Mathios E Mylonakis, Maria Kritsepikonstantinou
    Abstract:

    Abstract Despite the advances in canine medicine and the rapid gaining of attention of canine models in biomedical field and particularly in Hemoglobin genes research, the studies on canine Hemoglobin composition are sparse with ambiguous findings. Our aim was: i) to investigate the electrophoretic pattern of canine Hemoglobin and the possible effect of age, sex, and anemia using a capillary Electrophoresis assay, and ii) to validate this assay and calculate reference intervals (RIs) for canine Hemoglobin fractions. Blood samples were collected from 53 healthy and 42 dogs with regenerative and non-regenerative anemias. The Sebia Capillarys 2 flex-piercing was used for Hemoglobin analysis and it was validated using canine blood samples. R statistical language was employed for the statistical analyses. A major Hemoglobin fraction (named HbA0) and a minor one (named HbA2) were identified in 100% and 47.4% of samples, respectively. The within-run and between-run CV was 0.1% for HbA0 and 9.1% and 11.2% for HbA2, respectively. The extremely narrow range of HbA0 and HbA2 values hampered a linearity study using canine blood samples. The RIs for HbA0 and HbA2 were 98.9-100% and 0-1.1%, respectively. HbA0 and HbA2 values were not correlated with age (P=0.866). No differences were observed in the median HbA0 and HbA2 between the two sexes (P=0.823), and healthy and anemic dogs (P=0.805). In conclusion, the capillary Electrophoresis revealed a major Hemoglobin fraction and an inconsistently present minor fraction. No effect of age, sex, or anemia was detected. The assay used was validated and RIs were generated, so as to be suitable for use in future investigations.

Mathios E Mylonakis - One of the best experts on this subject based on the ideXlab platform.

  • capillary Hemoglobin Electrophoresis of healthy and anemic dogs quantification validation and reference intervals of Hemoglobin fractions
    PLOS ONE, 2019
    Co-Authors: Ioannis L Oikonomidis, Theodora K Tsouloufi, Mathios E Mylonakis, Maria Kritsepikonstantinou
    Abstract:

    : Despite the advances in canine medicine and the rapid gaining of attention of canine models in biomedical field and particularly in Hemoglobin genes research, the studies on canine Hemoglobin composition are sparse with ambiguous findings. Our aim was: i) to investigate the electrophoretic pattern of canine Hemoglobin and the possible effects of age, sex, and anemia using a capillary Electrophoresis assay, and ii) to validate this assay and calculate reference intervals (RIs) for canine Hemoglobin fractions. Blood samples were collected from 53 healthy and 42 dogs with regenerative and non-regenerative anemias. The Sebia Capillarys 2 flex-piercing was used for Hemoglobin analysis and it was validated using canine blood samples. R statistical language was employed for the statistical analyses. A major Hemoglobin fraction (named HbA0) and a minor one (named HbA2) were identified in 100% and 47.4% of samples, respectively. The within-run and between-run CV was 0.1% for HbA0, and 9.1% and 11.2% for HbA2, respectively. The extremely narrow range of HbA0 and HbA2 values hampered a linearity study using canine blood samples. The RIs for HbA0 and HbA2 were 98.9-100% and 0-1.1%, respectively. HbA0 and HbA2 values were not significantly correlated with age (P = 0.866) or reticulocyte count (P = 0.731). No differences were observed in the median HbA0 and HbA2 between the two sexes (P = 0.887), and healthy and anemic dogs (P = 0.805). In conclusion, the capillary Electrophoresis revealed a major Hemoglobin fraction and an inconsistently present minor fraction. No effect of age, sex, anemia, or regenerative status of anemia was detected. The assay used was validated and RIs were generated, so as to be suitable for use in future investigations.

  • capillary Hemoglobin Electrophoresis of healthy and anemic dogs quantification validation and reference intervals of Hemoglobin fractions
    bioRxiv, 2019
    Co-Authors: Ioannis L Oikonomidis, Theodora K Tsouloufi, Mathios E Mylonakis, Maria Kritsepikonstantinou
    Abstract:

    Abstract Despite the advances in canine medicine and the rapid gaining of attention of canine models in biomedical field and particularly in Hemoglobin genes research, the studies on canine Hemoglobin composition are sparse with ambiguous findings. Our aim was: i) to investigate the electrophoretic pattern of canine Hemoglobin and the possible effect of age, sex, and anemia using a capillary Electrophoresis assay, and ii) to validate this assay and calculate reference intervals (RIs) for canine Hemoglobin fractions. Blood samples were collected from 53 healthy and 42 dogs with regenerative and non-regenerative anemias. The Sebia Capillarys 2 flex-piercing was used for Hemoglobin analysis and it was validated using canine blood samples. R statistical language was employed for the statistical analyses. A major Hemoglobin fraction (named HbA0) and a minor one (named HbA2) were identified in 100% and 47.4% of samples, respectively. The within-run and between-run CV was 0.1% for HbA0 and 9.1% and 11.2% for HbA2, respectively. The extremely narrow range of HbA0 and HbA2 values hampered a linearity study using canine blood samples. The RIs for HbA0 and HbA2 were 98.9-100% and 0-1.1%, respectively. HbA0 and HbA2 values were not correlated with age (P=0.866). No differences were observed in the median HbA0 and HbA2 between the two sexes (P=0.823), and healthy and anemic dogs (P=0.805). In conclusion, the capillary Electrophoresis revealed a major Hemoglobin fraction and an inconsistently present minor fraction. No effect of age, sex, or anemia was detected. The assay used was validated and RIs were generated, so as to be suitable for use in future investigations.

Theodora K Tsouloufi - One of the best experts on this subject based on the ideXlab platform.

  • capillary Hemoglobin Electrophoresis of healthy and anemic dogs quantification validation and reference intervals of Hemoglobin fractions
    PLOS ONE, 2019
    Co-Authors: Ioannis L Oikonomidis, Theodora K Tsouloufi, Mathios E Mylonakis, Maria Kritsepikonstantinou
    Abstract:

    : Despite the advances in canine medicine and the rapid gaining of attention of canine models in biomedical field and particularly in Hemoglobin genes research, the studies on canine Hemoglobin composition are sparse with ambiguous findings. Our aim was: i) to investigate the electrophoretic pattern of canine Hemoglobin and the possible effects of age, sex, and anemia using a capillary Electrophoresis assay, and ii) to validate this assay and calculate reference intervals (RIs) for canine Hemoglobin fractions. Blood samples were collected from 53 healthy and 42 dogs with regenerative and non-regenerative anemias. The Sebia Capillarys 2 flex-piercing was used for Hemoglobin analysis and it was validated using canine blood samples. R statistical language was employed for the statistical analyses. A major Hemoglobin fraction (named HbA0) and a minor one (named HbA2) were identified in 100% and 47.4% of samples, respectively. The within-run and between-run CV was 0.1% for HbA0, and 9.1% and 11.2% for HbA2, respectively. The extremely narrow range of HbA0 and HbA2 values hampered a linearity study using canine blood samples. The RIs for HbA0 and HbA2 were 98.9-100% and 0-1.1%, respectively. HbA0 and HbA2 values were not significantly correlated with age (P = 0.866) or reticulocyte count (P = 0.731). No differences were observed in the median HbA0 and HbA2 between the two sexes (P = 0.887), and healthy and anemic dogs (P = 0.805). In conclusion, the capillary Electrophoresis revealed a major Hemoglobin fraction and an inconsistently present minor fraction. No effect of age, sex, anemia, or regenerative status of anemia was detected. The assay used was validated and RIs were generated, so as to be suitable for use in future investigations.

  • capillary Hemoglobin Electrophoresis of healthy and anemic dogs quantification validation and reference intervals of Hemoglobin fractions
    bioRxiv, 2019
    Co-Authors: Ioannis L Oikonomidis, Theodora K Tsouloufi, Mathios E Mylonakis, Maria Kritsepikonstantinou
    Abstract:

    Abstract Despite the advances in canine medicine and the rapid gaining of attention of canine models in biomedical field and particularly in Hemoglobin genes research, the studies on canine Hemoglobin composition are sparse with ambiguous findings. Our aim was: i) to investigate the electrophoretic pattern of canine Hemoglobin and the possible effect of age, sex, and anemia using a capillary Electrophoresis assay, and ii) to validate this assay and calculate reference intervals (RIs) for canine Hemoglobin fractions. Blood samples were collected from 53 healthy and 42 dogs with regenerative and non-regenerative anemias. The Sebia Capillarys 2 flex-piercing was used for Hemoglobin analysis and it was validated using canine blood samples. R statistical language was employed for the statistical analyses. A major Hemoglobin fraction (named HbA0) and a minor one (named HbA2) were identified in 100% and 47.4% of samples, respectively. The within-run and between-run CV was 0.1% for HbA0 and 9.1% and 11.2% for HbA2, respectively. The extremely narrow range of HbA0 and HbA2 values hampered a linearity study using canine blood samples. The RIs for HbA0 and HbA2 were 98.9-100% and 0-1.1%, respectively. HbA0 and HbA2 values were not correlated with age (P=0.866). No differences were observed in the median HbA0 and HbA2 between the two sexes (P=0.823), and healthy and anemic dogs (P=0.805). In conclusion, the capillary Electrophoresis revealed a major Hemoglobin fraction and an inconsistently present minor fraction. No effect of age, sex, or anemia was detected. The assay used was validated and RIs were generated, so as to be suitable for use in future investigations.

Pongsak Charnngam - One of the best experts on this subject based on the ideXlab platform.

  • The prevalence and risk factors of anemia in pregnant women.
    Journal of the Medical Association of Thailand Chotmaihet thangphaet, 2003
    Co-Authors: Pichai Chotnopparatpattara, Sompop Limpongsanurak, Pongsak Charnngam
    Abstract:

    Objective: To determine the prevalence and risk factors of anemia in pregnant women. Method: The pregnant women were screened with complete blood count, Hemoglobin Electrophoresis and serology tests for hepatitis B, syphilis and HIV. In cases of anemia, serum for iron and ferritin were investigated. Anemia was defined as a Hemoglobin level less than 11.0 g/dl in the first and third trimester of pregnancy or less than 10.5 g/dl in the second trimester. Factors associated with anemia were analyzed by using student's t-test and chi-square. The statistically significant factors were tested with the use of multiple logistic regression. Results: A total of 1,304 pregnant women were recruited. The prevalence of anemia was 19.2 per cent (251 cases). Classified in each trimester, the prevalence was 14.8 per cent, 20.5 per cent and 38.6 per cent in the first, second and third trimester, respectively. One hundred and sixty-one cases of anemia were available for serum iron and ferritin levels. Iron deficiency anemia, by means of serum ferritin, was detected in 32 cases (19.9%). An abnormal pattern of Hemoglobin Electrophoresis was detected in 367 cases (28.1%). Gestational age at first prenatal visit, abnormal Hemoglobin Electrophoresis and educational status were associated with anemia during pregnancy. Conclusion: The prevalence of anemia in pregnant women who first attended the prenatal visit was 19.2 per cent. Factors associated with anemia during pregnancy were gestational age at first prenatal visit, abnormal Hemoglobin Electrophoresis and educational status.