The Experts below are selected from a list of 447 Experts worldwide ranked by ideXlab platform

Hideo Honjo - One of the best experts on this subject based on the ideXlab platform.

  • quantitative analysis of cellular fetal Hemoglobin Gamma Chain messenger rna hbf γ mrna in maternal peripheral blood
    Prenatal Diagnosis, 2004
    Co-Authors: Tomohiro Okuda, Yoshiyuki Kinoshita, Takaya Tamura, C. Kato, H. Kojima, Ai Watanabe, Hideo Honjo
    Abstract:

    Objective Fetal cells cross the feto-maternal barrier and circulate in maternal peripheral blood; thus, this study aimed to show the relationship between clinical evidence in pregnancy and qualitative feto-maternal barrier changes. Methods The expression of fetal Hemoglobin Gamma Chain messenger RNA (HbF-γ mRNA) was measured by quantitative reverse transcriptase-polymerase Chain reaction (RT-PCR) in maternal peripheral blood. Results HbF-γ mRNA was detected in all pregnant women after 5 weeks of gestation. In normal pregnancy, there were two peaks at 10 and 40 weeks, and a significant increase 4 weeks prior to the onset of labor. In intrauterine growth restriction (IUGR), preterm delivery and placenta previa, the HbF-γ mRNA expression was significantly higher than in normal pregnancies. Conclusion The expression of HbF-γ mRNA relative to that of β-actin mRNA is thought to reflect the real-time leakage of fetal cells into maternal blood. Copyright © 2004 John Wiley & Sons, Ltd.

  • Expression of IL‐4, IL‐8 and IL‐18 Messenger RNAs in Maternal Peripheral Blood and Relationships with the HbF‐γ Chain mRNA in it
    American journal of reproductive immunology (New York N.Y. : 1989), 2004
    Co-Authors: C. Kato, Tomohiro Okuda, Yoshiyuki Kinoshita, Takaya Tamura, H. Kojima, Hideo Honjo
    Abstract:

    Problem:  This study was designed to examine immunological changes in maternal peripheral blood and the relationship of these changes with the amount of fetal cells in the blood. Method of study:  The expression of interleukin-4 (IL-4), IL-8, IL-18 and fetal Hemoglobin Gamma Chain (HbF-γ Chain) messenger RNAs (mRNAs) in maternal peripheral blood was measured by a quantitative reverse transcription-polymerase Chain reaction method. Results:  In maternal peripheral blood, the expression of IL-4 mRNA was up-regulated from the second gestational month (GM) to delivery. The expression of IL-8 and IL-18 mRNAs was down-regulated from the third or fourth GM until the eighth or ninth GM, respectively, and both increased before the onset of labor, though IL-4 mRNA decreased. The expression of IL-8 and IL-18, but not IL-4, mRNAs was correlated with that of HbF-γ Chain mRNA. Conclusions:  Immunological interactions between maternal peripheral immune cells and fetal cells appear to be related to the onset of labor.

  • Quantitative analysis of cellular fetal Hemoglobin Gamma Chain messenger RNA (HbF‐γ mRNA) in maternal peripheral blood
    Prenatal diagnosis, 2004
    Co-Authors: Tomohiro Okuda, Yoshiyuki Kinoshita, Takaya Tamura, C. Kato, H. Kojima, Ai Watanabe, Hideo Honjo
    Abstract:

    Objective Fetal cells cross the feto-maternal barrier and circulate in maternal peripheral blood; thus, this study aimed to show the relationship between clinical evidence in pregnancy and qualitative feto-maternal barrier changes. Methods The expression of fetal Hemoglobin Gamma Chain messenger RNA (HbF-γ mRNA) was measured by quantitative reverse transcriptase-polymerase Chain reaction (RT-PCR) in maternal peripheral blood. Results HbF-γ mRNA was detected in all pregnant women after 5 weeks of gestation. In normal pregnancy, there were two peaks at 10 and 40 weeks, and a significant increase 4 weeks prior to the onset of labor. In intrauterine growth restriction (IUGR), preterm delivery and placenta previa, the HbF-γ mRNA expression was significantly higher than in normal pregnancies. Conclusion The expression of HbF-γ mRNA relative to that of β-actin mRNA is thought to reflect the real-time leakage of fetal cells into maternal blood. Copyright © 2004 John Wiley & Sons, Ltd.

Tomohiro Okuda - One of the best experts on this subject based on the ideXlab platform.

  • quantitative analysis of cellular fetal Hemoglobin Gamma Chain messenger rna hbf γ mrna in maternal peripheral blood
    Prenatal Diagnosis, 2004
    Co-Authors: Tomohiro Okuda, Yoshiyuki Kinoshita, Takaya Tamura, C. Kato, H. Kojima, Ai Watanabe, Hideo Honjo
    Abstract:

    Objective Fetal cells cross the feto-maternal barrier and circulate in maternal peripheral blood; thus, this study aimed to show the relationship between clinical evidence in pregnancy and qualitative feto-maternal barrier changes. Methods The expression of fetal Hemoglobin Gamma Chain messenger RNA (HbF-γ mRNA) was measured by quantitative reverse transcriptase-polymerase Chain reaction (RT-PCR) in maternal peripheral blood. Results HbF-γ mRNA was detected in all pregnant women after 5 weeks of gestation. In normal pregnancy, there were two peaks at 10 and 40 weeks, and a significant increase 4 weeks prior to the onset of labor. In intrauterine growth restriction (IUGR), preterm delivery and placenta previa, the HbF-γ mRNA expression was significantly higher than in normal pregnancies. Conclusion The expression of HbF-γ mRNA relative to that of β-actin mRNA is thought to reflect the real-time leakage of fetal cells into maternal blood. Copyright © 2004 John Wiley & Sons, Ltd.

  • Expression of IL‐4, IL‐8 and IL‐18 Messenger RNAs in Maternal Peripheral Blood and Relationships with the HbF‐γ Chain mRNA in it
    American journal of reproductive immunology (New York N.Y. : 1989), 2004
    Co-Authors: C. Kato, Tomohiro Okuda, Yoshiyuki Kinoshita, Takaya Tamura, H. Kojima, Hideo Honjo
    Abstract:

    Problem:  This study was designed to examine immunological changes in maternal peripheral blood and the relationship of these changes with the amount of fetal cells in the blood. Method of study:  The expression of interleukin-4 (IL-4), IL-8, IL-18 and fetal Hemoglobin Gamma Chain (HbF-γ Chain) messenger RNAs (mRNAs) in maternal peripheral blood was measured by a quantitative reverse transcription-polymerase Chain reaction method. Results:  In maternal peripheral blood, the expression of IL-4 mRNA was up-regulated from the second gestational month (GM) to delivery. The expression of IL-8 and IL-18 mRNAs was down-regulated from the third or fourth GM until the eighth or ninth GM, respectively, and both increased before the onset of labor, though IL-4 mRNA decreased. The expression of IL-8 and IL-18, but not IL-4, mRNAs was correlated with that of HbF-γ Chain mRNA. Conclusions:  Immunological interactions between maternal peripheral immune cells and fetal cells appear to be related to the onset of labor.

  • Quantitative analysis of cellular fetal Hemoglobin Gamma Chain messenger RNA (HbF‐γ mRNA) in maternal peripheral blood
    Prenatal diagnosis, 2004
    Co-Authors: Tomohiro Okuda, Yoshiyuki Kinoshita, Takaya Tamura, C. Kato, H. Kojima, Ai Watanabe, Hideo Honjo
    Abstract:

    Objective Fetal cells cross the feto-maternal barrier and circulate in maternal peripheral blood; thus, this study aimed to show the relationship between clinical evidence in pregnancy and qualitative feto-maternal barrier changes. Methods The expression of fetal Hemoglobin Gamma Chain messenger RNA (HbF-γ mRNA) was measured by quantitative reverse transcriptase-polymerase Chain reaction (RT-PCR) in maternal peripheral blood. Results HbF-γ mRNA was detected in all pregnant women after 5 weeks of gestation. In normal pregnancy, there were two peaks at 10 and 40 weeks, and a significant increase 4 weeks prior to the onset of labor. In intrauterine growth restriction (IUGR), preterm delivery and placenta previa, the HbF-γ mRNA expression was significantly higher than in normal pregnancies. Conclusion The expression of HbF-γ mRNA relative to that of β-actin mRNA is thought to reflect the real-time leakage of fetal cells into maternal blood. Copyright © 2004 John Wiley & Sons, Ltd.

C. Kato - One of the best experts on this subject based on the ideXlab platform.

  • quantitative analysis of cellular fetal Hemoglobin Gamma Chain messenger rna hbf γ mrna in maternal peripheral blood
    Prenatal Diagnosis, 2004
    Co-Authors: Tomohiro Okuda, Yoshiyuki Kinoshita, Takaya Tamura, C. Kato, H. Kojima, Ai Watanabe, Hideo Honjo
    Abstract:

    Objective Fetal cells cross the feto-maternal barrier and circulate in maternal peripheral blood; thus, this study aimed to show the relationship between clinical evidence in pregnancy and qualitative feto-maternal barrier changes. Methods The expression of fetal Hemoglobin Gamma Chain messenger RNA (HbF-γ mRNA) was measured by quantitative reverse transcriptase-polymerase Chain reaction (RT-PCR) in maternal peripheral blood. Results HbF-γ mRNA was detected in all pregnant women after 5 weeks of gestation. In normal pregnancy, there were two peaks at 10 and 40 weeks, and a significant increase 4 weeks prior to the onset of labor. In intrauterine growth restriction (IUGR), preterm delivery and placenta previa, the HbF-γ mRNA expression was significantly higher than in normal pregnancies. Conclusion The expression of HbF-γ mRNA relative to that of β-actin mRNA is thought to reflect the real-time leakage of fetal cells into maternal blood. Copyright © 2004 John Wiley & Sons, Ltd.

  • Expression of IL‐4, IL‐8 and IL‐18 Messenger RNAs in Maternal Peripheral Blood and Relationships with the HbF‐γ Chain mRNA in it
    American journal of reproductive immunology (New York N.Y. : 1989), 2004
    Co-Authors: C. Kato, Tomohiro Okuda, Yoshiyuki Kinoshita, Takaya Tamura, H. Kojima, Hideo Honjo
    Abstract:

    Problem:  This study was designed to examine immunological changes in maternal peripheral blood and the relationship of these changes with the amount of fetal cells in the blood. Method of study:  The expression of interleukin-4 (IL-4), IL-8, IL-18 and fetal Hemoglobin Gamma Chain (HbF-γ Chain) messenger RNAs (mRNAs) in maternal peripheral blood was measured by a quantitative reverse transcription-polymerase Chain reaction method. Results:  In maternal peripheral blood, the expression of IL-4 mRNA was up-regulated from the second gestational month (GM) to delivery. The expression of IL-8 and IL-18 mRNAs was down-regulated from the third or fourth GM until the eighth or ninth GM, respectively, and both increased before the onset of labor, though IL-4 mRNA decreased. The expression of IL-8 and IL-18, but not IL-4, mRNAs was correlated with that of HbF-γ Chain mRNA. Conclusions:  Immunological interactions between maternal peripheral immune cells and fetal cells appear to be related to the onset of labor.

  • Quantitative analysis of cellular fetal Hemoglobin Gamma Chain messenger RNA (HbF‐γ mRNA) in maternal peripheral blood
    Prenatal diagnosis, 2004
    Co-Authors: Tomohiro Okuda, Yoshiyuki Kinoshita, Takaya Tamura, C. Kato, H. Kojima, Ai Watanabe, Hideo Honjo
    Abstract:

    Objective Fetal cells cross the feto-maternal barrier and circulate in maternal peripheral blood; thus, this study aimed to show the relationship between clinical evidence in pregnancy and qualitative feto-maternal barrier changes. Methods The expression of fetal Hemoglobin Gamma Chain messenger RNA (HbF-γ mRNA) was measured by quantitative reverse transcriptase-polymerase Chain reaction (RT-PCR) in maternal peripheral blood. Results HbF-γ mRNA was detected in all pregnant women after 5 weeks of gestation. In normal pregnancy, there were two peaks at 10 and 40 weeks, and a significant increase 4 weeks prior to the onset of labor. In intrauterine growth restriction (IUGR), preterm delivery and placenta previa, the HbF-γ mRNA expression was significantly higher than in normal pregnancies. Conclusion The expression of HbF-γ mRNA relative to that of β-actin mRNA is thought to reflect the real-time leakage of fetal cells into maternal blood. Copyright © 2004 John Wiley & Sons, Ltd.

Takaya Tamura - One of the best experts on this subject based on the ideXlab platform.

  • quantitative analysis of cellular fetal Hemoglobin Gamma Chain messenger rna hbf γ mrna in maternal peripheral blood
    Prenatal Diagnosis, 2004
    Co-Authors: Tomohiro Okuda, Yoshiyuki Kinoshita, Takaya Tamura, C. Kato, H. Kojima, Ai Watanabe, Hideo Honjo
    Abstract:

    Objective Fetal cells cross the feto-maternal barrier and circulate in maternal peripheral blood; thus, this study aimed to show the relationship between clinical evidence in pregnancy and qualitative feto-maternal barrier changes. Methods The expression of fetal Hemoglobin Gamma Chain messenger RNA (HbF-γ mRNA) was measured by quantitative reverse transcriptase-polymerase Chain reaction (RT-PCR) in maternal peripheral blood. Results HbF-γ mRNA was detected in all pregnant women after 5 weeks of gestation. In normal pregnancy, there were two peaks at 10 and 40 weeks, and a significant increase 4 weeks prior to the onset of labor. In intrauterine growth restriction (IUGR), preterm delivery and placenta previa, the HbF-γ mRNA expression was significantly higher than in normal pregnancies. Conclusion The expression of HbF-γ mRNA relative to that of β-actin mRNA is thought to reflect the real-time leakage of fetal cells into maternal blood. Copyright © 2004 John Wiley & Sons, Ltd.

  • Expression of IL‐4, IL‐8 and IL‐18 Messenger RNAs in Maternal Peripheral Blood and Relationships with the HbF‐γ Chain mRNA in it
    American journal of reproductive immunology (New York N.Y. : 1989), 2004
    Co-Authors: C. Kato, Tomohiro Okuda, Yoshiyuki Kinoshita, Takaya Tamura, H. Kojima, Hideo Honjo
    Abstract:

    Problem:  This study was designed to examine immunological changes in maternal peripheral blood and the relationship of these changes with the amount of fetal cells in the blood. Method of study:  The expression of interleukin-4 (IL-4), IL-8, IL-18 and fetal Hemoglobin Gamma Chain (HbF-γ Chain) messenger RNAs (mRNAs) in maternal peripheral blood was measured by a quantitative reverse transcription-polymerase Chain reaction method. Results:  In maternal peripheral blood, the expression of IL-4 mRNA was up-regulated from the second gestational month (GM) to delivery. The expression of IL-8 and IL-18 mRNAs was down-regulated from the third or fourth GM until the eighth or ninth GM, respectively, and both increased before the onset of labor, though IL-4 mRNA decreased. The expression of IL-8 and IL-18, but not IL-4, mRNAs was correlated with that of HbF-γ Chain mRNA. Conclusions:  Immunological interactions between maternal peripheral immune cells and fetal cells appear to be related to the onset of labor.

  • Quantitative analysis of cellular fetal Hemoglobin Gamma Chain messenger RNA (HbF‐γ mRNA) in maternal peripheral blood
    Prenatal diagnosis, 2004
    Co-Authors: Tomohiro Okuda, Yoshiyuki Kinoshita, Takaya Tamura, C. Kato, H. Kojima, Ai Watanabe, Hideo Honjo
    Abstract:

    Objective Fetal cells cross the feto-maternal barrier and circulate in maternal peripheral blood; thus, this study aimed to show the relationship between clinical evidence in pregnancy and qualitative feto-maternal barrier changes. Methods The expression of fetal Hemoglobin Gamma Chain messenger RNA (HbF-γ mRNA) was measured by quantitative reverse transcriptase-polymerase Chain reaction (RT-PCR) in maternal peripheral blood. Results HbF-γ mRNA was detected in all pregnant women after 5 weeks of gestation. In normal pregnancy, there were two peaks at 10 and 40 weeks, and a significant increase 4 weeks prior to the onset of labor. In intrauterine growth restriction (IUGR), preterm delivery and placenta previa, the HbF-γ mRNA expression was significantly higher than in normal pregnancies. Conclusion The expression of HbF-γ mRNA relative to that of β-actin mRNA is thought to reflect the real-time leakage of fetal cells into maternal blood. Copyright © 2004 John Wiley & Sons, Ltd.

H. Kojima - One of the best experts on this subject based on the ideXlab platform.

  • quantitative analysis of cellular fetal Hemoglobin Gamma Chain messenger rna hbf γ mrna in maternal peripheral blood
    Prenatal Diagnosis, 2004
    Co-Authors: Tomohiro Okuda, Yoshiyuki Kinoshita, Takaya Tamura, C. Kato, H. Kojima, Ai Watanabe, Hideo Honjo
    Abstract:

    Objective Fetal cells cross the feto-maternal barrier and circulate in maternal peripheral blood; thus, this study aimed to show the relationship between clinical evidence in pregnancy and qualitative feto-maternal barrier changes. Methods The expression of fetal Hemoglobin Gamma Chain messenger RNA (HbF-γ mRNA) was measured by quantitative reverse transcriptase-polymerase Chain reaction (RT-PCR) in maternal peripheral blood. Results HbF-γ mRNA was detected in all pregnant women after 5 weeks of gestation. In normal pregnancy, there were two peaks at 10 and 40 weeks, and a significant increase 4 weeks prior to the onset of labor. In intrauterine growth restriction (IUGR), preterm delivery and placenta previa, the HbF-γ mRNA expression was significantly higher than in normal pregnancies. Conclusion The expression of HbF-γ mRNA relative to that of β-actin mRNA is thought to reflect the real-time leakage of fetal cells into maternal blood. Copyright © 2004 John Wiley & Sons, Ltd.

  • Expression of IL‐4, IL‐8 and IL‐18 Messenger RNAs in Maternal Peripheral Blood and Relationships with the HbF‐γ Chain mRNA in it
    American journal of reproductive immunology (New York N.Y. : 1989), 2004
    Co-Authors: C. Kato, Tomohiro Okuda, Yoshiyuki Kinoshita, Takaya Tamura, H. Kojima, Hideo Honjo
    Abstract:

    Problem:  This study was designed to examine immunological changes in maternal peripheral blood and the relationship of these changes with the amount of fetal cells in the blood. Method of study:  The expression of interleukin-4 (IL-4), IL-8, IL-18 and fetal Hemoglobin Gamma Chain (HbF-γ Chain) messenger RNAs (mRNAs) in maternal peripheral blood was measured by a quantitative reverse transcription-polymerase Chain reaction method. Results:  In maternal peripheral blood, the expression of IL-4 mRNA was up-regulated from the second gestational month (GM) to delivery. The expression of IL-8 and IL-18 mRNAs was down-regulated from the third or fourth GM until the eighth or ninth GM, respectively, and both increased before the onset of labor, though IL-4 mRNA decreased. The expression of IL-8 and IL-18, but not IL-4, mRNAs was correlated with that of HbF-γ Chain mRNA. Conclusions:  Immunological interactions between maternal peripheral immune cells and fetal cells appear to be related to the onset of labor.

  • Quantitative analysis of cellular fetal Hemoglobin Gamma Chain messenger RNA (HbF‐γ mRNA) in maternal peripheral blood
    Prenatal diagnosis, 2004
    Co-Authors: Tomohiro Okuda, Yoshiyuki Kinoshita, Takaya Tamura, C. Kato, H. Kojima, Ai Watanabe, Hideo Honjo
    Abstract:

    Objective Fetal cells cross the feto-maternal barrier and circulate in maternal peripheral blood; thus, this study aimed to show the relationship between clinical evidence in pregnancy and qualitative feto-maternal barrier changes. Methods The expression of fetal Hemoglobin Gamma Chain messenger RNA (HbF-γ mRNA) was measured by quantitative reverse transcriptase-polymerase Chain reaction (RT-PCR) in maternal peripheral blood. Results HbF-γ mRNA was detected in all pregnant women after 5 weeks of gestation. In normal pregnancy, there were two peaks at 10 and 40 weeks, and a significant increase 4 weeks prior to the onset of labor. In intrauterine growth restriction (IUGR), preterm delivery and placenta previa, the HbF-γ mRNA expression was significantly higher than in normal pregnancies. Conclusion The expression of HbF-γ mRNA relative to that of β-actin mRNA is thought to reflect the real-time leakage of fetal cells into maternal blood. Copyright © 2004 John Wiley & Sons, Ltd.