The Experts below are selected from a list of 384 Experts worldwide ranked by ideXlab platform
Michael R. Hamblin - One of the best experts on this subject based on the ideXlab platform.
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Chitosan Acetate Bandage as a Topical Antimicrobial Dressing for Infected Burns
Antimicrobial agents and chemotherapy, 2008Co-Authors: Tianhong Dai, George P. Tegos, Marina Burkatovskaya, Ana P. Castano, Michael R. HamblinAbstract:An engineered chitosan acetate bandage preparation (HemCon) is used as a Hemostatic Dressing, and its chemical structure suggests that it should also be antimicrobial. We previously showed that when a chitosan acetate bandage was applied to full-thickness excisional wounds in mice that had been infected with pathogenic bioluminescent bacteria (Pseudomonas aeruginosa, Proteus mirabilis, and Staphylococcus aureus), it was able to rapidly kill the bacteria and save the mice from developing fatal infections. Wound healing was also stimulated. In the present study, we asked whether a chitosan acetate bandage could act as a topical antimicrobial Dressing when it was applied to third-degree burns in mice contaminated with two of these bacterial species (P. aeruginosa and P. mirabilis). Preliminary experiments established the length of burn time and the number of bacteria needed to produce fatal infections in untreated mice and established that the chitosan acetate bandage could adhere to the infected burn for up to 21 days. In the case of P. aeruginosa infections, the survival rate of mice treated with the chitosan acetate bandage was 73.3% (whereas the survival rate of mice treated with a nanocrystalline silver Dressing was 27.3% [P = 0.0055] and that of untreated mice was 13.3% [P < 0.0002]). For P. mirabilis infections, the comparable survival rates were 66.7%, 62.5%, and 23.1% respectively. Quantitative bioluminescent signals showed that the chitosan acetate bandage effectively controlled the growth of bacteria in the burn and prevented the development of systemic sepsis, as shown by blood culture. These data suggest that chitosan acetate bandage is efficacious in preventing fatal burn infections.
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effect of chitosan acetate bandage on wound healing in infected and noninfected wounds in mice
Wound Repair and Regeneration, 2008Co-Authors: Marina Burkatovskaya, George P. Tegos, Ana P. Castano, Michael R. Hamblin, Tatiana N DemidovariceAbstract:HemCon s bandage is an engineered chitosan acetate preparation designed as a Hemostatic Dressing, and is under investigation as a topical antimicrobial Dressing. We studied its effects on healing of excisional wounds that were or were not infected with Staphylococcus aureus, in normal mice or mice previously pretreated with cyclophosphamide (CY). CY significantly suppressed wound healing in both the early and later stages, while S. aureus alone significantly stimulated wound healing in the early stages by preventing the initial wound expansion. CY plus S. aureus showed an advantage in early stages by preventing expansion, but a significant slowing of wound healing in later stages. In order to study the conflicting clamping and stimulating effects of chitosan acetate bandage on normal wounds, we removed the bandage from wounds at times after application ranging from 1 hour to 9 days. Three days application gave the earliest wound closure, and all application times gave a faster healing slope after removal compared with control wounds. Chitosan acetate bandage reduced the number of inflammatory cells in the wound at days 2 and 4, and had an overall beneficial effect on wound healing especially during the early period where its antimicrobial effect is most important.
Jong Chul Park - One of the best experts on this subject based on the ideXlab platform.
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recombinant batroxobin coated nonwoven chitosan as Hemostatic Dressing for initial hemorrhage control
International Journal of Biological Macromolecules, 2018Co-Authors: Gyeung Mi Seon, Mi Hee Lee, Byeong Ju Kwon, Min Sung Kim, Min Ah Koo, Young Seomun, Jong Tak Kim, Tae Hee Kim, Jong Chul ParkAbstract:The choice of hemostat is determined by the situation and the degree of hemorrhage. One common hemostat, the nonwoven Dressing, is easy to handled and controls severe bleeding on wider wounds. In this study, chitosan-based nonwoven Dressings with recombinant batroxobin (rBat) were used as efficacious Hemostatic Dressing agents. Hemostatic agents need to absorb blood quickly in the early stages of blood coagulation cascade to rapidly and effectively control of excessive hemorrhages. To date, most studies of Hemostatic agents focused on a single material and hemostats composed of multiple materials have not been studied sufficiently. Thus, we made a chitosan Dressing coated with rBat and investigated the microstructure, mechanical properties, Hemostatic efficacy, and clotting properties of the coated Dressing. Our results showed that the rBat had a synergetic effect on chitosan that improved blood coagulation. Furthermore, the Dressing had excellent bleeding control in an Sprague-Dawley (SD) rat femoral artery hemorrhage model. In conclusion, hemostasis can be improved by combining a chitosan-based nonwoven Dressing with other agents, and rBat-coated chitosan-based nonwoven Dressings have enormous potential to improve blood coagulation.
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functional improvement of Hemostatic Dressing by addition of recombinant batroxobin
Acta Biomaterialia, 2017Co-Authors: Gyeung Mi Seon, Mi Hee Lee, Byeong Ju Kwon, Min Sung Kim, Min Ah Koo, Young Seomun, Jong Tak Kim, Do Hyun Kim, Jong Chul ParkAbstract:Abstract Although a number of natural materials have been used as Hemostatic agents, many substances do not act quickly enough. Here, we created a novel Dressings using collagen and chitosan with recombinant batroxobin (r-Bat) to promote faster and more effective hemostasis. We hypothesized that r-Bat would promote synergetic blood coagulation because it contains a blood coagulation active site different than those of collagen and chitosan. Our results suggest that each substances can maintain Hemostatic properties while in the mixed Dressings and that our novel Hemostatic Dressings promotes potent control of bleeding, as demonstrated by a whole blood assay and rat hemorrhage model. In a rat femoral artery model, the scaffold with a high r-Bat concentration more rapidly controlled excessive bleeding. This novel Dressings has enormous possible for rapidly controlling bleeding and it improves upon the effect of collagen and chitosan used alone. Our novel r-Bat Dressings is a possible candidate for improving preoperative care and displays promising properties as an absorbable agent in hemostasis. Statement of Significance Despite the excellent Hemostatic properties of collagen and chitosan pads, they reported to brittle behavior and lack sufficient Hemostatic effect within relevant time. Therefore, we created a novel pad using collagen and chitosan with recombinant batroxobin (r-Bat). r-Bat acts as a thrombin-like enzyme in the coagulation cascade. Specifically, r-Bat, in contrast to thrombin, only splits fibrinopeptide A off and does not influence other Hemostatic factors or cells, which makes it clinically useful as a stable Hemostatic agent. Also the materials in the pad have synergetic effect because they have different Hemostatic mechanisms in the coagulation cascade. This report propose the novel Hemostatic pad isreasonable that a great potential for excessive bleeding injury and improve effects of natural substance Hemostatic pad.
Marina Burkatovskaya - One of the best experts on this subject based on the ideXlab platform.
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Chitosan Acetate Bandage as a Topical Antimicrobial Dressing for Infected Burns
Antimicrobial agents and chemotherapy, 2008Co-Authors: Tianhong Dai, George P. Tegos, Marina Burkatovskaya, Ana P. Castano, Michael R. HamblinAbstract:An engineered chitosan acetate bandage preparation (HemCon) is used as a Hemostatic Dressing, and its chemical structure suggests that it should also be antimicrobial. We previously showed that when a chitosan acetate bandage was applied to full-thickness excisional wounds in mice that had been infected with pathogenic bioluminescent bacteria (Pseudomonas aeruginosa, Proteus mirabilis, and Staphylococcus aureus), it was able to rapidly kill the bacteria and save the mice from developing fatal infections. Wound healing was also stimulated. In the present study, we asked whether a chitosan acetate bandage could act as a topical antimicrobial Dressing when it was applied to third-degree burns in mice contaminated with two of these bacterial species (P. aeruginosa and P. mirabilis). Preliminary experiments established the length of burn time and the number of bacteria needed to produce fatal infections in untreated mice and established that the chitosan acetate bandage could adhere to the infected burn for up to 21 days. In the case of P. aeruginosa infections, the survival rate of mice treated with the chitosan acetate bandage was 73.3% (whereas the survival rate of mice treated with a nanocrystalline silver Dressing was 27.3% [P = 0.0055] and that of untreated mice was 13.3% [P < 0.0002]). For P. mirabilis infections, the comparable survival rates were 66.7%, 62.5%, and 23.1% respectively. Quantitative bioluminescent signals showed that the chitosan acetate bandage effectively controlled the growth of bacteria in the burn and prevented the development of systemic sepsis, as shown by blood culture. These data suggest that chitosan acetate bandage is efficacious in preventing fatal burn infections.
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effect of chitosan acetate bandage on wound healing in infected and noninfected wounds in mice
Wound Repair and Regeneration, 2008Co-Authors: Marina Burkatovskaya, George P. Tegos, Ana P. Castano, Michael R. Hamblin, Tatiana N DemidovariceAbstract:HemCon s bandage is an engineered chitosan acetate preparation designed as a Hemostatic Dressing, and is under investigation as a topical antimicrobial Dressing. We studied its effects on healing of excisional wounds that were or were not infected with Staphylococcus aureus, in normal mice or mice previously pretreated with cyclophosphamide (CY). CY significantly suppressed wound healing in both the early and later stages, while S. aureus alone significantly stimulated wound healing in the early stages by preventing the initial wound expansion. CY plus S. aureus showed an advantage in early stages by preventing expansion, but a significant slowing of wound healing in later stages. In order to study the conflicting clamping and stimulating effects of chitosan acetate bandage on normal wounds, we removed the bandage from wounds at times after application ranging from 1 hour to 9 days. Three days application gave the earliest wound closure, and all application times gave a faster healing slope after removal compared with control wounds. Chitosan acetate bandage reduced the number of inflammatory cells in the wound at days 2 and 4, and had an overall beneficial effect on wound healing especially during the early period where its antimicrobial effect is most important.
Gyeung Mi Seon - One of the best experts on this subject based on the ideXlab platform.
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recombinant batroxobin coated nonwoven chitosan as Hemostatic Dressing for initial hemorrhage control
International Journal of Biological Macromolecules, 2018Co-Authors: Gyeung Mi Seon, Mi Hee Lee, Byeong Ju Kwon, Min Sung Kim, Min Ah Koo, Young Seomun, Jong Tak Kim, Tae Hee Kim, Jong Chul ParkAbstract:The choice of hemostat is determined by the situation and the degree of hemorrhage. One common hemostat, the nonwoven Dressing, is easy to handled and controls severe bleeding on wider wounds. In this study, chitosan-based nonwoven Dressings with recombinant batroxobin (rBat) were used as efficacious Hemostatic Dressing agents. Hemostatic agents need to absorb blood quickly in the early stages of blood coagulation cascade to rapidly and effectively control of excessive hemorrhages. To date, most studies of Hemostatic agents focused on a single material and hemostats composed of multiple materials have not been studied sufficiently. Thus, we made a chitosan Dressing coated with rBat and investigated the microstructure, mechanical properties, Hemostatic efficacy, and clotting properties of the coated Dressing. Our results showed that the rBat had a synergetic effect on chitosan that improved blood coagulation. Furthermore, the Dressing had excellent bleeding control in an Sprague-Dawley (SD) rat femoral artery hemorrhage model. In conclusion, hemostasis can be improved by combining a chitosan-based nonwoven Dressing with other agents, and rBat-coated chitosan-based nonwoven Dressings have enormous potential to improve blood coagulation.
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functional improvement of Hemostatic Dressing by addition of recombinant batroxobin
Acta Biomaterialia, 2017Co-Authors: Gyeung Mi Seon, Mi Hee Lee, Byeong Ju Kwon, Min Sung Kim, Min Ah Koo, Young Seomun, Jong Tak Kim, Do Hyun Kim, Jong Chul ParkAbstract:Abstract Although a number of natural materials have been used as Hemostatic agents, many substances do not act quickly enough. Here, we created a novel Dressings using collagen and chitosan with recombinant batroxobin (r-Bat) to promote faster and more effective hemostasis. We hypothesized that r-Bat would promote synergetic blood coagulation because it contains a blood coagulation active site different than those of collagen and chitosan. Our results suggest that each substances can maintain Hemostatic properties while in the mixed Dressings and that our novel Hemostatic Dressings promotes potent control of bleeding, as demonstrated by a whole blood assay and rat hemorrhage model. In a rat femoral artery model, the scaffold with a high r-Bat concentration more rapidly controlled excessive bleeding. This novel Dressings has enormous possible for rapidly controlling bleeding and it improves upon the effect of collagen and chitosan used alone. Our novel r-Bat Dressings is a possible candidate for improving preoperative care and displays promising properties as an absorbable agent in hemostasis. Statement of Significance Despite the excellent Hemostatic properties of collagen and chitosan pads, they reported to brittle behavior and lack sufficient Hemostatic effect within relevant time. Therefore, we created a novel pad using collagen and chitosan with recombinant batroxobin (r-Bat). r-Bat acts as a thrombin-like enzyme in the coagulation cascade. Specifically, r-Bat, in contrast to thrombin, only splits fibrinopeptide A off and does not influence other Hemostatic factors or cells, which makes it clinically useful as a stable Hemostatic agent. Also the materials in the pad have synergetic effect because they have different Hemostatic mechanisms in the coagulation cascade. This report propose the novel Hemostatic pad isreasonable that a great potential for excessive bleeding injury and improve effects of natural substance Hemostatic pad.
Ana P. Castano - One of the best experts on this subject based on the ideXlab platform.
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Chitosan Acetate Bandage as a Topical Antimicrobial Dressing for Infected Burns
Antimicrobial agents and chemotherapy, 2008Co-Authors: Tianhong Dai, George P. Tegos, Marina Burkatovskaya, Ana P. Castano, Michael R. HamblinAbstract:An engineered chitosan acetate bandage preparation (HemCon) is used as a Hemostatic Dressing, and its chemical structure suggests that it should also be antimicrobial. We previously showed that when a chitosan acetate bandage was applied to full-thickness excisional wounds in mice that had been infected with pathogenic bioluminescent bacteria (Pseudomonas aeruginosa, Proteus mirabilis, and Staphylococcus aureus), it was able to rapidly kill the bacteria and save the mice from developing fatal infections. Wound healing was also stimulated. In the present study, we asked whether a chitosan acetate bandage could act as a topical antimicrobial Dressing when it was applied to third-degree burns in mice contaminated with two of these bacterial species (P. aeruginosa and P. mirabilis). Preliminary experiments established the length of burn time and the number of bacteria needed to produce fatal infections in untreated mice and established that the chitosan acetate bandage could adhere to the infected burn for up to 21 days. In the case of P. aeruginosa infections, the survival rate of mice treated with the chitosan acetate bandage was 73.3% (whereas the survival rate of mice treated with a nanocrystalline silver Dressing was 27.3% [P = 0.0055] and that of untreated mice was 13.3% [P < 0.0002]). For P. mirabilis infections, the comparable survival rates were 66.7%, 62.5%, and 23.1% respectively. Quantitative bioluminescent signals showed that the chitosan acetate bandage effectively controlled the growth of bacteria in the burn and prevented the development of systemic sepsis, as shown by blood culture. These data suggest that chitosan acetate bandage is efficacious in preventing fatal burn infections.
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effect of chitosan acetate bandage on wound healing in infected and noninfected wounds in mice
Wound Repair and Regeneration, 2008Co-Authors: Marina Burkatovskaya, George P. Tegos, Ana P. Castano, Michael R. Hamblin, Tatiana N DemidovariceAbstract:HemCon s bandage is an engineered chitosan acetate preparation designed as a Hemostatic Dressing, and is under investigation as a topical antimicrobial Dressing. We studied its effects on healing of excisional wounds that were or were not infected with Staphylococcus aureus, in normal mice or mice previously pretreated with cyclophosphamide (CY). CY significantly suppressed wound healing in both the early and later stages, while S. aureus alone significantly stimulated wound healing in the early stages by preventing the initial wound expansion. CY plus S. aureus showed an advantage in early stages by preventing expansion, but a significant slowing of wound healing in later stages. In order to study the conflicting clamping and stimulating effects of chitosan acetate bandage on normal wounds, we removed the bandage from wounds at times after application ranging from 1 hour to 9 days. Three days application gave the earliest wound closure, and all application times gave a faster healing slope after removal compared with control wounds. Chitosan acetate bandage reduced the number of inflammatory cells in the wound at days 2 and 4, and had an overall beneficial effect on wound healing especially during the early period where its antimicrobial effect is most important.