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Anders Kiessling - One of the best experts on this subject based on the ideXlab platform.

  • Validation of the Hepatic Portal Vein cannulation technique using Atlantic salmon Salmo salar L.
    Journal of Fish Biology, 2007
    Co-Authors: Erika J. Eliason, Anders Karlsson, Anders Kiessling, B. Djordjevic, Anthony P. Farrell
    Abstract:

    This study assessed the Hepatic Portal Vein cannulation technique and surgical recovery in Atlantic salmon Salmo salar. Haematocrit levels were maintained and blood variables, including cortisol, returned to baseline levels within 1–3 days post-surgery, indicating that this technique is a viable, useful method to study the digestive physiology of fishes.

  • Postprandial changes in plasma free amino acid levels obtained simultaneously from the Hepatic Portal Vein and the dorsal aorta in rainbow trout (Oncorhynchus mykiss).
    The Journal of Experimental Biology, 2006
    Co-Authors: Anders Karlsson, Erika J. Eliason, Anthony P. Farrell, Liv Torunn Mydland, Anders Kiessling
    Abstract:

    SUMMARY For the first time, changes in plasma concentrations of free amino acid (AA) and their metabolites were followed simultaneously in pre- and post-Hepatic blood following a single meal in non-anaesthetized and free-swimming fish. Rainbow trout ( Oncorhynchus mykiss ), kept in 10°C water and fitted with cannulae in the Hepatic Portal Vein (HPV) and the dorsal aorta (DA), were force-fed 1% of their body mass and blood samples were taken from both cannulae at 0, 3, 6, 12, 24 and 48 h postprandially to follow the free AA profile. Almost all free AAs increased rapidly within the first 3 h and only a few free AAs did not change significantly over time. By 6 h, the total free AA concentration had peaked in blood taken from both the DA (7107±369 nmol ml -1 ) and HPV (9999±572 nmol ml -1 ). However, individual free AAs showed three main profiles beyond this time: for type I, a peak concentration occurred only at 6 h; for type II, there was a more gradual rise in concentration to a peak at 24 h; and for type III there were two peaks, at 6 h and 24 h. All free AAs returned to or were lower than baseline levels within 48 h, with the exception of threonine and proline. The total free AA concentrations were consistently higher ( P

  • postprandial changes in plasma free amino acid levels obtained simultaneously from the Hepatic Portal Vein and the dorsal aorta in rainbow trout oncorhynchus mykiss
    The Journal of Experimental Biology, 2006
    Co-Authors: Anders Karlsson, Erika J. Eliason, Anthony P. Farrell, Liv Torunn Mydland, Anders Kiessling
    Abstract:

    SUMMARY For the first time, changes in plasma concentrations of free amino acid (AA) and their metabolites were followed simultaneously in pre- and post-Hepatic blood following a single meal in non-anaesthetized and free-swimming fish. Rainbow trout ( Oncorhynchus mykiss ), kept in 10°C water and fitted with cannulae in the Hepatic Portal Vein (HPV) and the dorsal aorta (DA), were force-fed 1% of their body mass and blood samples were taken from both cannulae at 0, 3, 6, 12, 24 and 48 h postprandially to follow the free AA profile. Almost all free AAs increased rapidly within the first 3 h and only a few free AAs did not change significantly over time. By 6 h, the total free AA concentration had peaked in blood taken from both the DA (7107±369 nmol ml -1 ) and HPV (9999±572 nmol ml -1 ). However, individual free AAs showed three main profiles beyond this time: for type I, a peak concentration occurred only at 6 h; for type II, there was a more gradual rise in concentration to a peak at 24 h; and for type III there were two peaks, at 6 h and 24 h. All free AAs returned to or were lower than baseline levels within 48 h, with the exception of threonine and proline. The total free AA concentrations were consistently higher ( P <0.05) in the HPV than in the DA at 3 h, 6 h, 12 h and 24 h. Our data provide clear evidence that, during the first pass through the liver, Hepatic modification altered individual free AA concentrations as indicated by variable ratios among the simultaneous blood samples. Furthermore, the elevation of ammonium and urea in the HPV indicates intestinal catabolism of ingested free AA before release into the HPV. In conclusion, the dual HPV and DA cannulation shows promise as a useful technique for qualitative and quantitative investigations of absorption and turnover of nutrients, especially if the measurements can be combined with reliable estimates of blood flow and labelled substances.

Anders Karlsson - One of the best experts on this subject based on the ideXlab platform.

  • Validation of the Hepatic Portal Vein cannulation technique using Atlantic salmon Salmo salar L.
    Journal of Fish Biology, 2007
    Co-Authors: Erika J. Eliason, Anders Karlsson, Anders Kiessling, B. Djordjevic, Anthony P. Farrell
    Abstract:

    This study assessed the Hepatic Portal Vein cannulation technique and surgical recovery in Atlantic salmon Salmo salar. Haematocrit levels were maintained and blood variables, including cortisol, returned to baseline levels within 1–3 days post-surgery, indicating that this technique is a viable, useful method to study the digestive physiology of fishes.

  • Postprandial changes in plasma free amino acid levels obtained simultaneously from the Hepatic Portal Vein and the dorsal aorta in rainbow trout (Oncorhynchus mykiss).
    The Journal of Experimental Biology, 2006
    Co-Authors: Anders Karlsson, Erika J. Eliason, Anthony P. Farrell, Liv Torunn Mydland, Anders Kiessling
    Abstract:

    SUMMARY For the first time, changes in plasma concentrations of free amino acid (AA) and their metabolites were followed simultaneously in pre- and post-Hepatic blood following a single meal in non-anaesthetized and free-swimming fish. Rainbow trout ( Oncorhynchus mykiss ), kept in 10°C water and fitted with cannulae in the Hepatic Portal Vein (HPV) and the dorsal aorta (DA), were force-fed 1% of their body mass and blood samples were taken from both cannulae at 0, 3, 6, 12, 24 and 48 h postprandially to follow the free AA profile. Almost all free AAs increased rapidly within the first 3 h and only a few free AAs did not change significantly over time. By 6 h, the total free AA concentration had peaked in blood taken from both the DA (7107±369 nmol ml -1 ) and HPV (9999±572 nmol ml -1 ). However, individual free AAs showed three main profiles beyond this time: for type I, a peak concentration occurred only at 6 h; for type II, there was a more gradual rise in concentration to a peak at 24 h; and for type III there were two peaks, at 6 h and 24 h. All free AAs returned to or were lower than baseline levels within 48 h, with the exception of threonine and proline. The total free AA concentrations were consistently higher ( P

  • postprandial changes in plasma free amino acid levels obtained simultaneously from the Hepatic Portal Vein and the dorsal aorta in rainbow trout oncorhynchus mykiss
    The Journal of Experimental Biology, 2006
    Co-Authors: Anders Karlsson, Erika J. Eliason, Anthony P. Farrell, Liv Torunn Mydland, Anders Kiessling
    Abstract:

    SUMMARY For the first time, changes in plasma concentrations of free amino acid (AA) and their metabolites were followed simultaneously in pre- and post-Hepatic blood following a single meal in non-anaesthetized and free-swimming fish. Rainbow trout ( Oncorhynchus mykiss ), kept in 10°C water and fitted with cannulae in the Hepatic Portal Vein (HPV) and the dorsal aorta (DA), were force-fed 1% of their body mass and blood samples were taken from both cannulae at 0, 3, 6, 12, 24 and 48 h postprandially to follow the free AA profile. Almost all free AAs increased rapidly within the first 3 h and only a few free AAs did not change significantly over time. By 6 h, the total free AA concentration had peaked in blood taken from both the DA (7107±369 nmol ml -1 ) and HPV (9999±572 nmol ml -1 ). However, individual free AAs showed three main profiles beyond this time: for type I, a peak concentration occurred only at 6 h; for type II, there was a more gradual rise in concentration to a peak at 24 h; and for type III there were two peaks, at 6 h and 24 h. All free AAs returned to or were lower than baseline levels within 48 h, with the exception of threonine and proline. The total free AA concentrations were consistently higher ( P <0.05) in the HPV than in the DA at 3 h, 6 h, 12 h and 24 h. Our data provide clear evidence that, during the first pass through the liver, Hepatic modification altered individual free AA concentrations as indicated by variable ratios among the simultaneous blood samples. Furthermore, the elevation of ammonium and urea in the HPV indicates intestinal catabolism of ingested free AA before release into the HPV. In conclusion, the dual HPV and DA cannulation shows promise as a useful technique for qualitative and quantitative investigations of absorption and turnover of nutrients, especially if the measurements can be combined with reliable estimates of blood flow and labelled substances.

Wolfgang Langhans - One of the best experts on this subject based on the ideXlab platform.

  • Hepatic-Portal Vein Infusions of Glucagon-Like Peptide-1 Reduce Meal Size and Increase c-Fos Expression in the Nucleus Tractus Solitarii, Area Postrema and Central Nucleus of the Amygdala in Rats
    Journal of neuroendocrinology, 2010
    Co-Authors: Isabelle Baumgartner, Myrtha Arnold, Wolfgang Langhans, Gustavo Pacheco-lópez, Elisabeth B. Rüttimann, Lori Asarian, Nori Geary, J.j.g. Hillebrand
    Abstract:

    We recently reported that brief, remotely controlled intrameal Hepatic-Portal Vein infusions of glucagon-like peptide-1 (GLP-1) reduced spontaneous meal size in rats. To investigate the neurobehavioural correlates of this effect, we equipped male Sprague-Dawley rats with Hepatic-Portal Vein catheters and assessed (i) the effect on eating of remotely triggered infusions of GLP-1 (1 nmol/kg, 5 min) or vehicle during the first nocturnal meal after 3 h of food deprivation and (ii) the effect of identical infusions performed at dark onset on c-Fos expression in several brain areas involved in the control of eating. GLP-1 reduced (P < 0.05) the size of the first nocturnal meal and increased its satiety ratio. Also, GLP-1 increased (P < 0.05) the number of c-Fos-expressing cells in the nucleus tractus solitarii, the area postrema and the central nucleus of the amygdala, but not in the arcuate or paraventricular hypothalamic nuclei. These data suggest that the nucleus tractus solitarii, the area postrema and the central nucleus of the amygdala play a role in the eating-inhibitory actions of GLP-1 infused into the Hepatic-Portal Vein; it remains to be established whether activation of these brain nuclei reflect satiation, aversion, or both.

  • Lipopolysaccharide-induced anorexia following Hepatic Portal Vein and vena cava administration
    Physiology & behavior, 1998
    Co-Authors: M. H. Porter, Myrtha Arnold, Wolfgang Langhans
    Abstract:

    To examine the role of the liver in lipopolysaccharide (LPS)-induced hypophagia, we investigated the effect of Hepatic Portal and vena cava infusions (1 mL/30 min) of LPS (100 microg/kg of body weight) on feeding in rats. LPS infusion significantly reduced food intake when administered via either the Hepatic Portal Vein or the vena cava. Both the magnitude and time course of this hypophagia were similar regardless of the infusion route. As in previous experiments of ours in which LPS was administered by intraperitoneal (i.p.) injection, LPS reduced food intake by decreasing meal frequency, without affecting meal size or duration. The results suggest that peripherally administered LPS does not act primarily in the liver to reduce food intake.

Erika J. Eliason - One of the best experts on this subject based on the ideXlab platform.

  • Validation of the Hepatic Portal Vein cannulation technique using Atlantic salmon Salmo salar L.
    Journal of Fish Biology, 2007
    Co-Authors: Erika J. Eliason, Anders Karlsson, Anders Kiessling, B. Djordjevic, Anthony P. Farrell
    Abstract:

    This study assessed the Hepatic Portal Vein cannulation technique and surgical recovery in Atlantic salmon Salmo salar. Haematocrit levels were maintained and blood variables, including cortisol, returned to baseline levels within 1–3 days post-surgery, indicating that this technique is a viable, useful method to study the digestive physiology of fishes.

  • Postprandial changes in plasma free amino acid levels obtained simultaneously from the Hepatic Portal Vein and the dorsal aorta in rainbow trout (Oncorhynchus mykiss).
    The Journal of Experimental Biology, 2006
    Co-Authors: Anders Karlsson, Erika J. Eliason, Anthony P. Farrell, Liv Torunn Mydland, Anders Kiessling
    Abstract:

    SUMMARY For the first time, changes in plasma concentrations of free amino acid (AA) and their metabolites were followed simultaneously in pre- and post-Hepatic blood following a single meal in non-anaesthetized and free-swimming fish. Rainbow trout ( Oncorhynchus mykiss ), kept in 10°C water and fitted with cannulae in the Hepatic Portal Vein (HPV) and the dorsal aorta (DA), were force-fed 1% of their body mass and blood samples were taken from both cannulae at 0, 3, 6, 12, 24 and 48 h postprandially to follow the free AA profile. Almost all free AAs increased rapidly within the first 3 h and only a few free AAs did not change significantly over time. By 6 h, the total free AA concentration had peaked in blood taken from both the DA (7107±369 nmol ml -1 ) and HPV (9999±572 nmol ml -1 ). However, individual free AAs showed three main profiles beyond this time: for type I, a peak concentration occurred only at 6 h; for type II, there was a more gradual rise in concentration to a peak at 24 h; and for type III there were two peaks, at 6 h and 24 h. All free AAs returned to or were lower than baseline levels within 48 h, with the exception of threonine and proline. The total free AA concentrations were consistently higher ( P

  • postprandial changes in plasma free amino acid levels obtained simultaneously from the Hepatic Portal Vein and the dorsal aorta in rainbow trout oncorhynchus mykiss
    The Journal of Experimental Biology, 2006
    Co-Authors: Anders Karlsson, Erika J. Eliason, Anthony P. Farrell, Liv Torunn Mydland, Anders Kiessling
    Abstract:

    SUMMARY For the first time, changes in plasma concentrations of free amino acid (AA) and their metabolites were followed simultaneously in pre- and post-Hepatic blood following a single meal in non-anaesthetized and free-swimming fish. Rainbow trout ( Oncorhynchus mykiss ), kept in 10°C water and fitted with cannulae in the Hepatic Portal Vein (HPV) and the dorsal aorta (DA), were force-fed 1% of their body mass and blood samples were taken from both cannulae at 0, 3, 6, 12, 24 and 48 h postprandially to follow the free AA profile. Almost all free AAs increased rapidly within the first 3 h and only a few free AAs did not change significantly over time. By 6 h, the total free AA concentration had peaked in blood taken from both the DA (7107±369 nmol ml -1 ) and HPV (9999±572 nmol ml -1 ). However, individual free AAs showed three main profiles beyond this time: for type I, a peak concentration occurred only at 6 h; for type II, there was a more gradual rise in concentration to a peak at 24 h; and for type III there were two peaks, at 6 h and 24 h. All free AAs returned to or were lower than baseline levels within 48 h, with the exception of threonine and proline. The total free AA concentrations were consistently higher ( P <0.05) in the HPV than in the DA at 3 h, 6 h, 12 h and 24 h. Our data provide clear evidence that, during the first pass through the liver, Hepatic modification altered individual free AA concentrations as indicated by variable ratios among the simultaneous blood samples. Furthermore, the elevation of ammonium and urea in the HPV indicates intestinal catabolism of ingested free AA before release into the HPV. In conclusion, the dual HPV and DA cannulation shows promise as a useful technique for qualitative and quantitative investigations of absorption and turnover of nutrients, especially if the measurements can be combined with reliable estimates of blood flow and labelled substances.

Anthony P. Farrell - One of the best experts on this subject based on the ideXlab platform.

  • Validation of the Hepatic Portal Vein cannulation technique using Atlantic salmon Salmo salar L.
    Journal of Fish Biology, 2007
    Co-Authors: Erika J. Eliason, Anders Karlsson, Anders Kiessling, B. Djordjevic, Anthony P. Farrell
    Abstract:

    This study assessed the Hepatic Portal Vein cannulation technique and surgical recovery in Atlantic salmon Salmo salar. Haematocrit levels were maintained and blood variables, including cortisol, returned to baseline levels within 1–3 days post-surgery, indicating that this technique is a viable, useful method to study the digestive physiology of fishes.

  • Postprandial changes in plasma free amino acid levels obtained simultaneously from the Hepatic Portal Vein and the dorsal aorta in rainbow trout (Oncorhynchus mykiss).
    The Journal of Experimental Biology, 2006
    Co-Authors: Anders Karlsson, Erika J. Eliason, Anthony P. Farrell, Liv Torunn Mydland, Anders Kiessling
    Abstract:

    SUMMARY For the first time, changes in plasma concentrations of free amino acid (AA) and their metabolites were followed simultaneously in pre- and post-Hepatic blood following a single meal in non-anaesthetized and free-swimming fish. Rainbow trout ( Oncorhynchus mykiss ), kept in 10°C water and fitted with cannulae in the Hepatic Portal Vein (HPV) and the dorsal aorta (DA), were force-fed 1% of their body mass and blood samples were taken from both cannulae at 0, 3, 6, 12, 24 and 48 h postprandially to follow the free AA profile. Almost all free AAs increased rapidly within the first 3 h and only a few free AAs did not change significantly over time. By 6 h, the total free AA concentration had peaked in blood taken from both the DA (7107±369 nmol ml -1 ) and HPV (9999±572 nmol ml -1 ). However, individual free AAs showed three main profiles beyond this time: for type I, a peak concentration occurred only at 6 h; for type II, there was a more gradual rise in concentration to a peak at 24 h; and for type III there were two peaks, at 6 h and 24 h. All free AAs returned to or were lower than baseline levels within 48 h, with the exception of threonine and proline. The total free AA concentrations were consistently higher ( P

  • postprandial changes in plasma free amino acid levels obtained simultaneously from the Hepatic Portal Vein and the dorsal aorta in rainbow trout oncorhynchus mykiss
    The Journal of Experimental Biology, 2006
    Co-Authors: Anders Karlsson, Erika J. Eliason, Anthony P. Farrell, Liv Torunn Mydland, Anders Kiessling
    Abstract:

    SUMMARY For the first time, changes in plasma concentrations of free amino acid (AA) and their metabolites were followed simultaneously in pre- and post-Hepatic blood following a single meal in non-anaesthetized and free-swimming fish. Rainbow trout ( Oncorhynchus mykiss ), kept in 10°C water and fitted with cannulae in the Hepatic Portal Vein (HPV) and the dorsal aorta (DA), were force-fed 1% of their body mass and blood samples were taken from both cannulae at 0, 3, 6, 12, 24 and 48 h postprandially to follow the free AA profile. Almost all free AAs increased rapidly within the first 3 h and only a few free AAs did not change significantly over time. By 6 h, the total free AA concentration had peaked in blood taken from both the DA (7107±369 nmol ml -1 ) and HPV (9999±572 nmol ml -1 ). However, individual free AAs showed three main profiles beyond this time: for type I, a peak concentration occurred only at 6 h; for type II, there was a more gradual rise in concentration to a peak at 24 h; and for type III there were two peaks, at 6 h and 24 h. All free AAs returned to or were lower than baseline levels within 48 h, with the exception of threonine and proline. The total free AA concentrations were consistently higher ( P <0.05) in the HPV than in the DA at 3 h, 6 h, 12 h and 24 h. Our data provide clear evidence that, during the first pass through the liver, Hepatic modification altered individual free AA concentrations as indicated by variable ratios among the simultaneous blood samples. Furthermore, the elevation of ammonium and urea in the HPV indicates intestinal catabolism of ingested free AA before release into the HPV. In conclusion, the dual HPV and DA cannulation shows promise as a useful technique for qualitative and quantitative investigations of absorption and turnover of nutrients, especially if the measurements can be combined with reliable estimates of blood flow and labelled substances.