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Adil Denizli - One of the best experts on this subject based on the ideXlab platform.

  • moleculAr imprinting bAsed composite cryogel membrAnes for purificAtion of Anti HepAtitis b surfAce Antibody by fAst protein liquid chromAtogrAphy
    Journal of Chromatography B, 2012
    Co-Authors: Sevgi Asliyuce, Lokman Uzun, Serhat Unal, Adil Denizli
    Abstract:

    AbstrAct In the present study, we hAve focused our Attention to prepAre moleculAr imprinted composite cryogel membrAnes for purificAtion of HepAtitis B surfAce Antibody (Anti-HBs) by fAst protein liquid chromAtogrAphy. Before the prepArAtion of the moleculAr imprinted composite cryogel membrAnes (MI-CMs) by free rAdicAl polymerizAtion At sub-zero temperAture, we hAve synthesized And chArActerized the Anti-HBs imprinted pArticles. Then, the cryogel membrAnes (CMs) were chArActerized by swelling test, scAnning electron microscopy And Fourier trAnsform infrAred spectroscopy. Prior to chromAtogrAphic purificAtion studies, the effective pArAmeters on the Anti-HBs Adsorption process were evAluAted by investigAting the dependency of the Adsorption cApAcity on flow-rAte, Anti-HBs concentrAtion, contAct time And ionic strength. The mAximum Anti-HBs Adsorption cApAcity wAs cAlculAted As 701.4 mIU/g CM. The selectivity of the MI-CMs wAs shown by competitive Adsorption of Anti-HBs, totAl Anti-HepAtitis A Antibody (Anti-HAV) And totAl immunoglobulin E (IgE) Adsorption studies. The MI-CMs hAve relAtive selectivity coefficients As 5.45 for Anti-HBs/totAl Anti-HAV And 9.05 for Anti-HBs/totAl IgE, respectively. The phosphAte buffer solution (pH 7.4) contAining 1.0 M NACl wAs used for elution, Almost completely, of Adsorbed Anti-HBs molecules. The MI-CMs could be used mAny times without Any significAnt decreAse in the Adsorption cApAcity. The chromAtogrAphic purificAtion performAnces of the MI-CMs were Also investigAted. The chromAtogrAphic pArAmeters such As cApAcity And sepArAtion fActors, the theoreticAl plAte number And resolution of the MI-CMs were cAlculAted As 5.48, 6.02, 1153.9, And 1.72 for Anti-HBs molecules, respectively. As A conclusion, we cAn sAy thAt the MI-CMs could be used for specific purificAtion of Anti-HBs from Anti-HBs positive humAn plAsmA.

  • HepAtitis b surfAce Antibody purificAtion with HepAtitis b surfAce Antibody imprinted poly hydroxyethyl methAcrylAte n methAcryloyl l tyrosine methyl ester pArticles
    Journal of Chromatography B, 2009
    Co-Authors: Lokman Uzun, Serhat Unal, Adil Denizli
    Abstract:

    AbstrAct HepAtitis B surfAce Antibody imprinted poly(hydroxyethyl methAcrylAte-N-methAcryloyl- l -tyrosine methyl ester) pArticles were prepAred for the purificAtion of HepAtitis B surfAce Antibody from humAn plAsmA. N-methAcryloyl- l -tyrosine methyl ester wAs chosen As A complexing Agent for HepAtitis B surfAce Antibodies. HepAtitis B surfAce Antibody imprinted poly(hydroxyethyl methAcrylAte-N-methAcryloyl- l -tyrosine methyl ester) pArticles were chArActerized by surfAce AreA meAsurements, swelling test, scAnning electron microscopy, elementAl AnAlysis, And Fourier trAnsform infrAred spectroscopy. Ethylene glycol (1.0 M) wAs used As desorption Agent. Adsorption studies were performed from HepAtitis B surfAce Antibody And Anti-HepAtitis A Antibody positive humAn plAsmA. Effects of Antibody concentrAtion, contAct time, N-methAcryloyl- l -tyrosine methyl ester content And temperAture on the Adsorption cApAcity were investigAted. The Amount of HepAtitis B surfAce Antibody Adsorbed per unit mAss increAsed with increAsing HepAtitis B surfAce Antibody concentrAtion, then reAched sAturAtion. MAximum HepAtitis B surfAce Antibody Adsorption Amount wAs 21.4 mIU/mg. Adsorption process reAched the equilibrium in 60 min. Competitive Adsorption of HepAtitis B surfAce Antibody, totAl Anti-HepAtitis A Antibody And totAl immunoglobulin E wAs investigAted for showing the selectivity. HepAtitis B surfAce Antibody-imprinted pArticles could Adsorb HepAtitis B surfAce Antibody 18.3 times more thAn Anti-HepAtitis A Antibody And 2.2 times more thAn immunoglobulin E. It cAn be concluded thAt HepAtitis B surfAce Antibody-imprinted pArticles hAve significAnt selectivity for HepAtitis B surfAce Antibody.

Lokman Uzun - One of the best experts on this subject based on the ideXlab platform.

  • moleculAr imprinting bAsed composite cryogel membrAnes for purificAtion of Anti HepAtitis b surfAce Antibody by fAst protein liquid chromAtogrAphy
    Journal of Chromatography B, 2012
    Co-Authors: Sevgi Asliyuce, Lokman Uzun, Serhat Unal, Adil Denizli
    Abstract:

    AbstrAct In the present study, we hAve focused our Attention to prepAre moleculAr imprinted composite cryogel membrAnes for purificAtion of HepAtitis B surfAce Antibody (Anti-HBs) by fAst protein liquid chromAtogrAphy. Before the prepArAtion of the moleculAr imprinted composite cryogel membrAnes (MI-CMs) by free rAdicAl polymerizAtion At sub-zero temperAture, we hAve synthesized And chArActerized the Anti-HBs imprinted pArticles. Then, the cryogel membrAnes (CMs) were chArActerized by swelling test, scAnning electron microscopy And Fourier trAnsform infrAred spectroscopy. Prior to chromAtogrAphic purificAtion studies, the effective pArAmeters on the Anti-HBs Adsorption process were evAluAted by investigAting the dependency of the Adsorption cApAcity on flow-rAte, Anti-HBs concentrAtion, contAct time And ionic strength. The mAximum Anti-HBs Adsorption cApAcity wAs cAlculAted As 701.4 mIU/g CM. The selectivity of the MI-CMs wAs shown by competitive Adsorption of Anti-HBs, totAl Anti-HepAtitis A Antibody (Anti-HAV) And totAl immunoglobulin E (IgE) Adsorption studies. The MI-CMs hAve relAtive selectivity coefficients As 5.45 for Anti-HBs/totAl Anti-HAV And 9.05 for Anti-HBs/totAl IgE, respectively. The phosphAte buffer solution (pH 7.4) contAining 1.0 M NACl wAs used for elution, Almost completely, of Adsorbed Anti-HBs molecules. The MI-CMs could be used mAny times without Any significAnt decreAse in the Adsorption cApAcity. The chromAtogrAphic purificAtion performAnces of the MI-CMs were Also investigAted. The chromAtogrAphic pArAmeters such As cApAcity And sepArAtion fActors, the theoreticAl plAte number And resolution of the MI-CMs were cAlculAted As 5.48, 6.02, 1153.9, And 1.72 for Anti-HBs molecules, respectively. As A conclusion, we cAn sAy thAt the MI-CMs could be used for specific purificAtion of Anti-HBs from Anti-HBs positive humAn plAsmA.

  • HepAtitis b surfAce Antibody purificAtion with HepAtitis b surfAce Antibody imprinted poly hydroxyethyl methAcrylAte n methAcryloyl l tyrosine methyl ester pArticles
    Journal of Chromatography B, 2009
    Co-Authors: Lokman Uzun, Serhat Unal, Adil Denizli
    Abstract:

    AbstrAct HepAtitis B surfAce Antibody imprinted poly(hydroxyethyl methAcrylAte-N-methAcryloyl- l -tyrosine methyl ester) pArticles were prepAred for the purificAtion of HepAtitis B surfAce Antibody from humAn plAsmA. N-methAcryloyl- l -tyrosine methyl ester wAs chosen As A complexing Agent for HepAtitis B surfAce Antibodies. HepAtitis B surfAce Antibody imprinted poly(hydroxyethyl methAcrylAte-N-methAcryloyl- l -tyrosine methyl ester) pArticles were chArActerized by surfAce AreA meAsurements, swelling test, scAnning electron microscopy, elementAl AnAlysis, And Fourier trAnsform infrAred spectroscopy. Ethylene glycol (1.0 M) wAs used As desorption Agent. Adsorption studies were performed from HepAtitis B surfAce Antibody And Anti-HepAtitis A Antibody positive humAn plAsmA. Effects of Antibody concentrAtion, contAct time, N-methAcryloyl- l -tyrosine methyl ester content And temperAture on the Adsorption cApAcity were investigAted. The Amount of HepAtitis B surfAce Antibody Adsorbed per unit mAss increAsed with increAsing HepAtitis B surfAce Antibody concentrAtion, then reAched sAturAtion. MAximum HepAtitis B surfAce Antibody Adsorption Amount wAs 21.4 mIU/mg. Adsorption process reAched the equilibrium in 60 min. Competitive Adsorption of HepAtitis B surfAce Antibody, totAl Anti-HepAtitis A Antibody And totAl immunoglobulin E wAs investigAted for showing the selectivity. HepAtitis B surfAce Antibody-imprinted pArticles could Adsorb HepAtitis B surfAce Antibody 18.3 times more thAn Anti-HepAtitis A Antibody And 2.2 times more thAn immunoglobulin E. It cAn be concluded thAt HepAtitis B surfAce Antibody-imprinted pArticles hAve significAnt selectivity for HepAtitis B surfAce Antibody.

Serhat Unal - One of the best experts on this subject based on the ideXlab platform.

  • moleculAr imprinting bAsed composite cryogel membrAnes for purificAtion of Anti HepAtitis b surfAce Antibody by fAst protein liquid chromAtogrAphy
    Journal of Chromatography B, 2012
    Co-Authors: Sevgi Asliyuce, Lokman Uzun, Serhat Unal, Adil Denizli
    Abstract:

    AbstrAct In the present study, we hAve focused our Attention to prepAre moleculAr imprinted composite cryogel membrAnes for purificAtion of HepAtitis B surfAce Antibody (Anti-HBs) by fAst protein liquid chromAtogrAphy. Before the prepArAtion of the moleculAr imprinted composite cryogel membrAnes (MI-CMs) by free rAdicAl polymerizAtion At sub-zero temperAture, we hAve synthesized And chArActerized the Anti-HBs imprinted pArticles. Then, the cryogel membrAnes (CMs) were chArActerized by swelling test, scAnning electron microscopy And Fourier trAnsform infrAred spectroscopy. Prior to chromAtogrAphic purificAtion studies, the effective pArAmeters on the Anti-HBs Adsorption process were evAluAted by investigAting the dependency of the Adsorption cApAcity on flow-rAte, Anti-HBs concentrAtion, contAct time And ionic strength. The mAximum Anti-HBs Adsorption cApAcity wAs cAlculAted As 701.4 mIU/g CM. The selectivity of the MI-CMs wAs shown by competitive Adsorption of Anti-HBs, totAl Anti-HepAtitis A Antibody (Anti-HAV) And totAl immunoglobulin E (IgE) Adsorption studies. The MI-CMs hAve relAtive selectivity coefficients As 5.45 for Anti-HBs/totAl Anti-HAV And 9.05 for Anti-HBs/totAl IgE, respectively. The phosphAte buffer solution (pH 7.4) contAining 1.0 M NACl wAs used for elution, Almost completely, of Adsorbed Anti-HBs molecules. The MI-CMs could be used mAny times without Any significAnt decreAse in the Adsorption cApAcity. The chromAtogrAphic purificAtion performAnces of the MI-CMs were Also investigAted. The chromAtogrAphic pArAmeters such As cApAcity And sepArAtion fActors, the theoreticAl plAte number And resolution of the MI-CMs were cAlculAted As 5.48, 6.02, 1153.9, And 1.72 for Anti-HBs molecules, respectively. As A conclusion, we cAn sAy thAt the MI-CMs could be used for specific purificAtion of Anti-HBs from Anti-HBs positive humAn plAsmA.

  • HepAtitis b surfAce Antibody purificAtion with HepAtitis b surfAce Antibody imprinted poly hydroxyethyl methAcrylAte n methAcryloyl l tyrosine methyl ester pArticles
    Journal of Chromatography B, 2009
    Co-Authors: Lokman Uzun, Serhat Unal, Adil Denizli
    Abstract:

    AbstrAct HepAtitis B surfAce Antibody imprinted poly(hydroxyethyl methAcrylAte-N-methAcryloyl- l -tyrosine methyl ester) pArticles were prepAred for the purificAtion of HepAtitis B surfAce Antibody from humAn plAsmA. N-methAcryloyl- l -tyrosine methyl ester wAs chosen As A complexing Agent for HepAtitis B surfAce Antibodies. HepAtitis B surfAce Antibody imprinted poly(hydroxyethyl methAcrylAte-N-methAcryloyl- l -tyrosine methyl ester) pArticles were chArActerized by surfAce AreA meAsurements, swelling test, scAnning electron microscopy, elementAl AnAlysis, And Fourier trAnsform infrAred spectroscopy. Ethylene glycol (1.0 M) wAs used As desorption Agent. Adsorption studies were performed from HepAtitis B surfAce Antibody And Anti-HepAtitis A Antibody positive humAn plAsmA. Effects of Antibody concentrAtion, contAct time, N-methAcryloyl- l -tyrosine methyl ester content And temperAture on the Adsorption cApAcity were investigAted. The Amount of HepAtitis B surfAce Antibody Adsorbed per unit mAss increAsed with increAsing HepAtitis B surfAce Antibody concentrAtion, then reAched sAturAtion. MAximum HepAtitis B surfAce Antibody Adsorption Amount wAs 21.4 mIU/mg. Adsorption process reAched the equilibrium in 60 min. Competitive Adsorption of HepAtitis B surfAce Antibody, totAl Anti-HepAtitis A Antibody And totAl immunoglobulin E wAs investigAted for showing the selectivity. HepAtitis B surfAce Antibody-imprinted pArticles could Adsorb HepAtitis B surfAce Antibody 18.3 times more thAn Anti-HepAtitis A Antibody And 2.2 times more thAn immunoglobulin E. It cAn be concluded thAt HepAtitis B surfAce Antibody-imprinted pArticles hAve significAnt selectivity for HepAtitis B surfAce Antibody.

H. Dele Davies - One of the best experts on this subject based on the ideXlab platform.

  • HepAtitis A: A preventAble threAt
    Advances in Therapy, 2005
    Co-Authors: Alexander K. C. Leung, James D. Kellner, H. Dele Davies
    Abstract:

    HepAtitis A is A mAjor public heAlth problem, pArticulArly in the pediAtric populAtion. Although HepAtitis A infection does not cAuse chronic liver diseAse, it is AssociAted with significAnt morbidity. The virus is trAnsmitted primArily by person-to-person contAct viA the fecAl-orAl route. The infection cAn be inAppArent, subclinicAl, Anicteric, or icteric. In generAl, the severity of the diseAse is inversely correlAted with the Age of the child. OccAsionAlly, fulminAnt HepAtitis, which is AssociAted with A high mortAlity rAte, mAy result. The diAgnosis of Acute HepAtitis A is most commonly mAde through the detection of immunoglobulin M (IgM) Anti-HepAtitis A Antibody. TreAtment is generAlly supportive. GenerAl preventive meAsures include improved stAndArds of hygiene And sAnitAtion. UniversAl childhood vAccinAtion is the most effective method for erAdicAting HepAtitis A And preventing its trAnsmission.

Sevgi Asliyuce - One of the best experts on this subject based on the ideXlab platform.

  • moleculAr imprinting bAsed composite cryogel membrAnes for purificAtion of Anti HepAtitis b surfAce Antibody by fAst protein liquid chromAtogrAphy
    Journal of Chromatography B, 2012
    Co-Authors: Sevgi Asliyuce, Lokman Uzun, Serhat Unal, Adil Denizli
    Abstract:

    AbstrAct In the present study, we hAve focused our Attention to prepAre moleculAr imprinted composite cryogel membrAnes for purificAtion of HepAtitis B surfAce Antibody (Anti-HBs) by fAst protein liquid chromAtogrAphy. Before the prepArAtion of the moleculAr imprinted composite cryogel membrAnes (MI-CMs) by free rAdicAl polymerizAtion At sub-zero temperAture, we hAve synthesized And chArActerized the Anti-HBs imprinted pArticles. Then, the cryogel membrAnes (CMs) were chArActerized by swelling test, scAnning electron microscopy And Fourier trAnsform infrAred spectroscopy. Prior to chromAtogrAphic purificAtion studies, the effective pArAmeters on the Anti-HBs Adsorption process were evAluAted by investigAting the dependency of the Adsorption cApAcity on flow-rAte, Anti-HBs concentrAtion, contAct time And ionic strength. The mAximum Anti-HBs Adsorption cApAcity wAs cAlculAted As 701.4 mIU/g CM. The selectivity of the MI-CMs wAs shown by competitive Adsorption of Anti-HBs, totAl Anti-HepAtitis A Antibody (Anti-HAV) And totAl immunoglobulin E (IgE) Adsorption studies. The MI-CMs hAve relAtive selectivity coefficients As 5.45 for Anti-HBs/totAl Anti-HAV And 9.05 for Anti-HBs/totAl IgE, respectively. The phosphAte buffer solution (pH 7.4) contAining 1.0 M NACl wAs used for elution, Almost completely, of Adsorbed Anti-HBs molecules. The MI-CMs could be used mAny times without Any significAnt decreAse in the Adsorption cApAcity. The chromAtogrAphic purificAtion performAnces of the MI-CMs were Also investigAted. The chromAtogrAphic pArAmeters such As cApAcity And sepArAtion fActors, the theoreticAl plAte number And resolution of the MI-CMs were cAlculAted As 5.48, 6.02, 1153.9, And 1.72 for Anti-HBs molecules, respectively. As A conclusion, we cAn sAy thAt the MI-CMs could be used for specific purificAtion of Anti-HBs from Anti-HBs positive humAn plAsmA.